The Influence of Packaging on Palatability and Shelf Life Stability of Horse Treats.

Horse treat packaging may be composed of materials including plastic and paper which protect the product from the environment to improve shelf life. Objectives of this research were to 1) assess the impact of packaging on shelf life of horse treats and 2) evaluate the impact of packaging on horse preferences. Three packaging treatments (control, poly, and paper) were examined at five time points over a 12 month period. Treatments were analyzed for moisture, water activity, mold, yeast, pH, and volatile organic acids. Horse preference testing evaluated first treatment sniffed, consumed, and finished as well as number of treats consumed. Significance was set at P < .05 and trends at P < .10. Moisture content and water activity increased in all treatments (P < .01) from month 0 to month 12, with paper packaging providing a greater fluctuation and containing visible mold at month 12 (P < .01). No difference was observed for first treatment sniffed, consumed, or finished during preference testing. However a trend (P = .09) for the period∗treatment interaction was observed for number of treats consumed, with poly increasing while paper decreased. These data indicate that packaging impacts shelf life and horse preference of treats.

Palatability of Horse Treats: Comparing the Preferences of Horses and Humans.

Despite its importance to product development, few data compare preferences for horses with human consumers. The objectives of this research were to compare treat preferences of horses against horse owners. Product A was a disk-shaped cinnamon-flavored flax-based treat, and product B was a textured apple-flavored oat-based treat. Horses were presented with two treat products in a paired preference test which comprised separate olfaction and consumption periods. Consumers evaluated the treats separately for purchase intent as well as hedonic testing of sensory attributes. No difference was observed for first product sniffed, consumed, or finished during the horse preference test. However, moderate positive correlations were observed between first product sniffed and consumed (P = .01, ф = 0.40) as well as first product consumed and finished (P < .01, ф = 0.48). Horse owners rated product A lower in appearance, texture, size, and purchase intent (P < .01) than product B. These results indicate that consumer testing for animal food should be considered during product development.

The Influence of Topically Applied Oil-Based Palatants on Eating Behavior in Horses.

Palatants may be added to equine feed and medication either during or after manufacturing to enhance product acceptance. Prior studies have examined a variety of palatants but results have been limited and inconsistent. Therefore, the objective of this research was to evaluate topically applied oil-based palatants on feeding preferences in horses. Stock-type horses (n = 10) were used in this paired preference test across a two-phase study. Phase one compared six palatants (banana, anise, peppermint, apple, spearmint, and orange) to a control (corn oil), whereas phase two compared preference among palatants (anise, apple, and peppermint). Feeding stocks were utilized and horses were allowed 15 seconds for olfaction followed by 3 minutes for consumption. Variables recorded included first diet sniffed and consumed, first action, aversive behaviors, excessive salivation, and consumption. Each trial was also video-recorded and number of chews were counted. Data were analyzed using chi-square and t tests in SAS version 9.4 with P < .05 established as significant. Findings from phase one reveal excessive salivation was observed less frequently (P < .05) for most palatants with the exception of peppermint and orange. Orange negatively impacted palatability indicated by less consumption when compared with the control (P = .02), although there was no impact on chews per gram. No difference between control and treatment diets for first sniff or first consumed was observed when analyzed individually in either phase one or two, although there was a moderate positive correlation (ф = 0.39, P = .04) between olfaction and consumption during the peppermint and anise comparison. Consumption as the first action was consistent across all trials (P < .05). Anise was preferred over apple and peppermint as indicated by higher total consumption (P < .05) in phase two. These data indicate that oil-based palatants can affect feed preferences in horses with increased palatability from anise and decreased palatability from orange flavors.

An Assessment of Decontamination Strategies for Materials Commonly Used in Canine Equipment.

Working canines are frequently exposed to hazardous environments with a high potential for contamination. Environmental contamination may occur in many ways. Contamination may be chemical, biological, radiological, or nuclear. Examples may include a pipeline rupture following an earthquake, microbiological contamination of floodwaters, or exposure to toxic industrial chemical such as hydrogen chloride, ammonia, or toluene. Evidence to support effective methods for decontamination of equipment commonly used by working canines is lacking. Recent work has identified decontamination protocols for working canines, but little data are available to guide the decontamination of equipment used during tactical operations. The objective of our work was to investigate the effects of cleanser, cleaning method, and material type on contaminant reduction for tactical canine equipment materials using an oil-based contaminant as a surrogate for toxic industrial chemical exposure. A contaminant was applied, and effectiveness was represented as either success (= 50% contaminant reduction) or failure (< 50% contaminant reduction). A two-phase study was used to investigate cleanser, method of cleaning, and material types for effective contaminant reduction. In phase 1, Simple Green® cleanser had a higher frequency (P = .0075) of failure, but method and material did not affect contamination reduction (P > .05). In phase 2, Dawn® (P = .0004) and Johnson's® (P = .0414) successfully reduced contamination. High-pressure cleaning (HPC) resulted in successful decontamination (P < .0001). These novel data demonstrate potential techniques for reduction of contaminants on tactical canine equipment.

Effect of Road Transport on the Equine Cecal Microbiota.

The effects of travel stress on the equine cecal microbiota are poorly understood. We hypothesized that travel would affect the equine cecal microbiota. Cecally-cannulated horses (n = 6) were randomly assigned to one of two groups, travel (n = 3) and control (n = 3). Horses received a basal diet (Strategy, Purina Animal Nutrition) with 1.2% body weight mixed grass/alfalfa. Travel horses were transported to an unfamiliar location, stalled to simulate weekend horse show conditions, and then returned to the Southern Illinois University Equine Center. Control horses remained at the equine center for the entire study. Cecal fluid was collected on a 6-hour rotating schedule, four times daily throughout the 6-day study. Data were analyzed using mixed models in SAS with P < .05. Cecal bacterial DNA was extracted, followed by 16S RNA sequencing and then analyzed using QIIME 1.8.0. Averages of sequence data were reported by phase (baseline, transportation, post-travel). Although there were no effects of travel associated with ß-diversity (P > .05), analysis of α-diversity measures indicated an effect within the travel group during the transportation phase as compared with baseline (P < .05). Interestingly, α-diversity was also affected for control horses in the return phase when compared to baseline. This may be due to the disruption of the return of the travel group. In addition, we identified multiple taxa affected by travel at both the genus and phylum level. Continued profiling of equine gastrointestinal microbiota is necessary to improve our understanding of equine microbial dysbiosis.