Trophic interactions modify the temperature dependence of community biomass and ecosystem function.

Aquatic ecosystems worldwide continue to experience unprecedented warming and ecological change. Warming increases metabolic rates of animals, plants, and microbes, accelerating their use of energy and materials, their population growth, and interaction rates. At a much larger biological scale, warming accelerates ecosystem-level processes, elevating fluxes of carbon and oxygen between biota and the atmosphere. Although these general effects of temperature at finer and broader biological scales are widely observed, they can lead to contradictory predictions for how warming affects the structure and function of ecological communities at the intermediate scale of biological organization. We experimentally tested the hypothesis that the presence of predators and their associated species interactions modify the temperature dependence of net ecosystem oxygen production and respiration. We tracked a series of independent freshwater ecosystems (370 L) over 9 weeks, and we found that at higher temperatures, cascading effects of predators on zooplankton prey and algae were stronger than at lower temperatures. When grazing was weak or absent, standing phytoplankton biomass declined by 85%-95% (<1-fold) over the temperature gradient (19-30 °C), and by 3-fold when grazers were present and lacked predators. These temperature-dependent species interactions and consequent community biomass shifts occurred without signs of species loss or community collapse, and only modestly affected the temperature dependence of net ecosystem oxygen fluxes. The exponential increases in net ecosystem oxygen production and consumption were relatively insensitive to differences in trophic interactions among ecosystems. Furthermore, monotonic declines in phytoplankton standing stock suggested no threshold effects of warming across systems. We conclude that local changes in community structure, including temperature-dependent trophic cascades, may be compatible with prevailing and predictable effects of temperature on ecosystem functions related to fundamental effects of temperature on metabolism.

Climate change affects low trophic level marine consumers: warming decreases copepod size and abundance.

Concern about climate change has re-ignited interest in universal ecological responses to temperature variations: (1) biogeographical shifts, (2) phenology changes, and (3) size shifts. In this study we used copepods as model organisms to study size responses to temperature because of their central role in the pelagic food web and because of the ontogenetic length constancy between molts, which facilitates the definition of size of distinct developmental stages. In order to test the expected temperature-induced shifts towards smaller body size and lower abundances under warming conditions, a mesocosm experiment using plankton from the Baltic Sea at three temperature levels (ambient, ambient +4 °C, ambient -4 °C) was performed in summer 2010. Overall copepod and copepodit abundances, copepod size at all life stages, and adult copepod size in particular, showed significant temperature effects. As expected, zooplankton peak abundance was lower in warm than in ambient treatments. Copepod size-at-immature stage significantly increased in cold treatments, while adult size significantly decreased in warm treatments.

CREATE'ing improvements in first-year students' science efficacy via an online introductory course experience.

With a primary objective to engage students in the process of science online, we transformed a long-standing laboratory course for first-year science students into a more accessible, immersive experience of current biological research using a narrow and focused set of primary literature and the Consider, Read, Elucidate a hypothesis, Analyze and interpret data, Think of the next Experiment (CREATE) pedagogy. The efficacy of the CREATE approach has been demonstrated in a diversity of higher education settings and courses. It is, however, not yet known if CREATE can be successfully implemented online with a large, diverse team of faculty untrained in the CREATE pedagogy. Here, we present the transformation of a large-enrollment, multi-section, multi-instructor course for first-year students in which the instructors follow different biological research questions but work together to reach shared goals and outcomes. We assessed students' (i) science self-efficacy and (ii) epistemological beliefs about science throughout an academic year of instruction fully administered online as a result of ongoing threats posed by COVID-19. Our findings demonstrate that novice CREATE instructors with varying levels of teaching experience and ranks can achieve comparable outcomes and improvements in students' science efficacy in the virtual classroom as a teaching team. This study extends the use of the CREATE pedagogy to large, team-taught, multi-section courses and shows its utility in the online teaching and learning environment.

Future climate change-related decreases in food quality may affect juvenile Chinook salmon growth and survival.

Global climate change is projected to raise global temperatures by 3.3-5.7 °C by 2100, resulting in changes in species composition, abundance, and nutritional quality of organisms at the base of the marine food web. Predicted increases in prey availability and reductions in prey nutritional quality under climate warming in certain marine systems are expected to impact higher trophic levels, such as fish and humans. There is limited knowledge of the interplay between food quantity and quality under warming, specifically when food availability is high, but quality is low. Here, we conducted an experiment assessing the effects of food quality (fatty acid composition and ratios) on juvenile Chinook salmon's (Oncorhynchus tshawytscha) body and nutritional condition, specifically focusing on RNA:DNA ratio, Fulton's K, growth, mortality and their fatty acid composition. Experimental diets represented three different climate change scenarios with 1) a present-day diet (Euphausia pacifica), 2) a control diet (commercial aquaculture diet), and 3) a predicted Intergovernmental Panel on Climate Change (IPCC) worst-case scenario diet with low essential fatty acid concentrations (IPCC SSP5-8.5). We tested how growth rates, RNA:DNA ratio, Fulton's K index, fatty acid composition and mortality rates in juvenile Chinook salmon compared across diet treatments. Fatty acids were incorporated into the salmon muscle at varying rates but, on average, reflected dietary concentrations. High dietary concentrations of DHA, EPA and high DHA:EPA ratios, under the control and present-day diets, increased fish growth and condition. In contrast, low concentrations of DHA and EPA and low DHA:EPA ratios in the diets under climate change scenario were not compensated for by increased food quantity. This result highlights the importance of considering food quality when assessing fish response to changing ocean conditions.

Combined Effects of Ocean Warming and Acidification on Copepod Abundance, Body Size and Fatty Acid Content.

Concerns about increasing atmospheric CO2 concentrations and global warming have initiated studies on the consequences of multiple-stressor interactions on marine organisms and ecosystems. We present a fully-crossed factorial mesocosm study and assess how warming and acidification affect the abundance, body size, and fatty acid composition of copepods as a measure of nutritional quality. The experimental set-up allowed us to determine whether the effects of warming and acidification act additively, synergistically, or antagonistically on the abundance, body size, and fatty acid content of copepods, a major group of lower level consumers in marine food webs. Copepodite (developmental stages 1-5) and nauplii abundance were antagonistically affected by warming and acidification. Higher temperature decreased copepodite and nauplii abundance, while acidification partially compensated for the temperature effect. The abundance of adult copepods was negatively affected by warming. The prosome length of copepods was significantly reduced by warming, and the interaction of warming and CO2 antagonistically affected prosome length. Fatty acid composition was also significantly affected by warming. The content of saturated fatty acids increased, and the ratios of the polyunsaturated essential fatty acids docosahexaenoic- (DHA) and arachidonic acid (ARA) to total fatty acid content increased with higher temperatures. Additionally, here was a significant additive interaction effect of both parameters on arachidonic acid. Our results indicate that in a future ocean scenario, acidification might partially counteract some observed effects of increased temperature on zooplankton, while adding to others. These may be results of a fertilizing effect on phytoplankton as a copepod food source. In summary, copepod populations will be more strongly affected by warming rather than by acidifying oceans, but ocean acidification effects can modify some temperature impacts.