RESUMEN
Simian foamy viruses (SFVs) are thought to infect virtually any adult nonhuman primate (NHP). While many data have accumulated about patterns of codivergence with their hosts and cross-species transmission events, little is known about the modalities of SFV transmission within NHP species, especially in the wild. Here we provide a detailed investigation of the dynamics of SFV circulation in a wild community of Western chimpanzees (Pan troglodytes verus). We demonstrate that mother-offspring (vertical) SFV transmission is common and hypothesize that it accounts for a number of primary infections. We also show that multiple infections with several chimpanzee-specific SFV strains (i.e., superinfection) commonly happen in adult chimpanzees, which might point to adult-specific aggressive behaviors as a lifelong source of SFV infection. Our data give evidence for complex SFV dynamics in wild chimpanzees, even at a single community scale, and show that linking wild NHP social interactions and their microorganisms' dynamics is feasible.
Asunto(s)
Enfermedades del Simio Antropoideo/transmisión , Transmisión Vertical de Enfermedad Infecciosa/veterinaria , Pan troglodytes/virología , Virus Espumoso de los Simios/fisiología , Factores de Edad , Animales , Animales Salvajes/virología , Enfermedades del Simio Antropoideo/virología , Femenino , Masculino , Linaje , Infecciones por Retroviridae/transmisión , Infecciones por Retroviridae/virologíaRESUMEN
Data are missing on the diversity of Plasmodium spp. infecting apes that live in their natural habitat, with limited possibility of human-mosquito-ape exchange. We surveyed Plasmodium spp. diversity in wild chimpanzees living in an undisturbed tropical rainforest habitat and found 5 species: P. malariae, P. vivax, P. ovale, P. reichenowi, and P. gaboni.
Asunto(s)
Enfermedades del Simio Antropoideo/epidemiología , Reservorios de Enfermedades/veterinaria , Monitoreo del Ambiente , Variación Genética , Malaria/veterinaria , Epidemiología Molecular , Pan troglodytes/parasitología , Plasmodium/clasificación , Animales , Enfermedades del Simio Antropoideo/parasitología , Secuencia de Bases , Côte d'Ivoire/epidemiología , Reservorios de Enfermedades/parasitología , Monitoreo Epidemiológico , Malaria/epidemiología , Datos de Secuencia Molecular , Filogenia , Plasmodium/genética , Plasmodium/aislamiento & purificación , Proteínas Protozoarias/genética , Árboles , Clima TropicalRESUMEN
One key question in social evolution is the identification of factors that promote the formation and maintenance of stable bonds between females and males beyond the mating context. Baboons lend themselves to examine this question, as they vary in social organisation and male-female association patterns. We report the results from the first systematic observations of individually identified wild female Guinea baboons. Guinea baboons live in a multilevel society with female-biased dispersal. Although several males could be found within 5 m of females, each female chiefly associated with one "primary" male at the 2 m distance. Social interactions occurred predominantly with the primary male, and female reproductive state had little influence on interaction patterns. The number of females per primary male varied from 1 to 4. During the 17-month study period, half of the females transferred between different males one or multiple times. A subset of females maintained weaker affiliative nonsexual relationships with other "secondary" males. Units composed of primary males with females, and occasional secondary males, apparently form the core of the Guinea baboon society. The social organisation and mating patterns of Guinea and hamadryas baboons may have a common evolutionary origin, despite notable differences in relationship quality. Specifically, Guinea baboon females appear to have greater leverage in their association patterns than hamadryas baboon females. Although we cannot yet explain the lack of overt male control over females, results generally support the notion that phylogenetic descent may play an important role in shaping social systems.
RESUMEN
While much attention has been focused on the molecular epidemiology of retroviruses in wild primate populations, the correlated question of the frequency and nature of super-infection events, i.e., the simultaneous infection of the same individual host with several strains of the same virus, has remained largely neglected. In particular, methods possibly allowing the investigation of super-infection from samples collected non-invasively (such as faeces) have never been properly compared. Here, we fill in this gap by assessing the costs and benefits of end-point dilution PCR (EPD-PCR) and multiple bulk-PCR cloning, as applied to a case study focusing on simian foamy virus super-infection in wild chimpanzees (Pan troglodytes). We show that, although considered to be the gold standard, EPD-PCR can lead to massive consumption of biological material when only low copy numbers of the target are expected. This constitutes a serious drawback in a field in which rarity of biological material is a fundamental constraint. In addition, we demonstrate that EPD-PCR results (single/multiple infection; founder strains) can be well predicted from multiple bulk-PCR clone experiments, by applying simple statistical and network analyses to sequence alignments. We therefore recommend the implementation of the latter method when the focus is put on retroviral super-infection and only low retroviral loads are encountered.