RESUMEN
Viruses that express reporter genes upon infection have been recently used to evaluate neutralizing antibody responses, where a lack of reporter expression indicates specific virus inhibition. The traditional model-based methods using standard outcome of percent neutralization could be applied to the data from the assays to estimate antibody titers. However, the data produced are sometimes irregular, which can yield meaningless outcomes of percent neutralization that do not fit the typical curves for immunoassays, making automated or semi-high throughput antibody titer estimation unreliable. We developed a type of new outcomes model, which is biologically meaningful and fits typical immunoassay curves well. Our simulation study indicates that the new response approach outperforms the traditional response approach regardless of the data variability. The proposed new response approach can be used in similar assays for other disease models.
Asunto(s)
Proteínas Fluorescentes Verdes/química , Pruebas de Neutralización/métodos , Anticuerpos Neutralizantes/análisis , Anticuerpos Antivirales/análisis , Glicoproteínas Hemaglutininas del Virus de la Influenza/análisis , Modelos Estadísticos , Método de MontecarloRESUMEN
Lingual thyroid should be considered in the differential diagnosis of any base of tongue mass. Examination may reveal worrisome obstructive findings. However, a majority of patients are hypothyroid and respond favorably to hormone replacement therapy upfront. Surgery is reserved for patients unresponsive to medical management or those with severe obstruction.
RESUMEN
Se presenta un paciente con Linfoma no Hodgkin linfocítico estadio IV y Retinosis Pigmentaria de base diagnosticado en el Centro de Referencia Nacional de Retinosis Pigmentaria, con manifestaciones oftalmológicas de esta neoplasia, remitido, además, al Instituto Nacional de Oncología y Radiobiología. Se ilustra con imágenes estas lesiones infrecuentes