Exploring the role of OXTR gene methylation in attachment development: A longitudinal study.

The current study explored longitudinally whether oxytocin receptor gene methylation (OXTRm) changes moderated the association between parental sensitivity changes and children's attachment changes over three waves. Six hundred six Flemish children (10-12 years, 42.8%-44.8% boys) completed attachment measures and provided salivary OXTRm data on seven CpG sites. Their parents reported their sensitive parenting. Results suggest that OXTRm changes hardly link to attachment (in)security changes after the age of 10. Some support was found for interaction effects between parental sensitivity changes and OXTRm changes on attachment changes over time. Effects suggest that for children with increased OXTRm in the promotor region and decreased methylation in the inhibitor region over time, increased parental sensitivity was associated with increased secure attachment and decreased insecure attachment over time.

Cumulative family stress and externalizing problems: Secure base script knowledge as a protective factor.

The current study examined whether secure base script knowledge can buffer against higher concurrent externalizing problems and against relative increases in externalizing problems associated with cumulative family stress. We conducted a one-year longitudinal study with two waves between 2017 and 2019 in which 272 Dutch-speaking Western European children from Flanders (47.8% boys, M age = 10.20 , SD age = 0.60 ) participated. Secure base script knowledge was associated with lower concurrent externalizing problems (f2  = 0.03). High levels of secure base script knowledge also buffered against relative increases in externalizing problems associated with cumulative family risk (f2  = 0.02). These findings suggest that secure base script knowledge can mitigate the negative effects of a stressful family environment on externalizing problems.

Perceived Parental Support and Psychological Control, DNA Methylation, and Loneliness: Longitudinal Associations Across Early Adolescence.

A broad range of factors have been associated with the development of adolescent loneliness. In the family context, a lack of parental support and high levels of parental psychological control have systematically been linked to loneliness. On the biological level, DNA methylation (which is an epigenetic process that suppresses gene expression) is believed to play a role in the development of loneliness. Specifically, high levels of DNA methylation in genes that play an important role in the functioning of the human stress response system are believed to elevate the risk of loneliness. Moreover, DNA methylation levels in these stress-related genes can be influenced by stressful environmental factors, suggesting a potential mediating role of DNA methylation in the association between parenting behaviors and loneliness. The current 3-year longitudinal study is the first study to examine the potential bidirectional longitudinal associations between loneliness, DNA methylation in stress-related genes, and both perceived parental support and psychological control. Furthermore, we explored the potential mediating role of DNA methylation in stress-related genes in the associations between perceived parenting and loneliness. The sample comprised 622 early adolescents (55% girls, Mage T1 = 10.77 years, SDage T1 = 0.48) who were followed from Grade 5 to 7. Parental support, psychological control, and loneliness were assessed annually by adolescent self-report questionnaires and DNA methylation was determined from saliva samples. Cross-Lagged Panel Models (CLPM) revealed that higher levels of loneliness predicted lower perceived parental support and higher perceived psychological control over time, as well as higher DNA methylation in some stress-related genes, that is, the glucocorticoid receptor gene (NR3C1) and the brain-derived neurotrophic factor (BDNF). In addition, higher NR3C1 methylation was predictive of lower perceived parental support and higher psychological control over time. No evidence was found for a mediating role of DNA methylation. Overall, our longitudinal findings challenge the current focus on DNA methylation and parenting behaviors as risk factors for adolescent loneliness. Instead, they suggest that the less considered direction of effects, which implies that loneliness predicts DNA methylation and aspects of parenting such as support and psychological control, should receive greater attention in future research.

Transactional Links Between Social Anxiety Symptoms and Parenting Across Adolescence: Between- and Within-Person Associations.

This 4-year longitudinal multi-informant study examined between- and within-person associations between adolescent social anxiety symptoms and parenting (parental psychological control and autonomy support). A community sample of 819 adolescents (46.1% girls; Mage T1  = 13.4 years) reported annually on social anxiety symptoms and both adolescents and mothers reported on parenting. Between-person associations suggested that adolescent social anxiety symptoms were associated with higher adolescent- and mother-reported psychological control and lower mother-reported autonomy support. At the within-person level, however, mothers reported lower psychological control and higher autonomy support after periods with higher adolescent social anxiety symptoms. Our findings illustrate the importance of distinguishing among between-person and within-person associations and including perceptions of both dyad members in longitudinal research concerning parenting and adolescent mental health.

Investigating the interplay between parenting dimensions and styles, and the association with adolescent outcomes.

Research has indicated that a strictly dimensional or parental style approach does not capture the full complexity of parenting. To better understand this complexity, the current study combined these two approaches using a novel statistical technique, i.e., subspace K-means clustering. Four objectives were addressed. First, the study tried to identify meaningful groups of parents in longitudinal adolescent reports on parenting behaviour. Second, the dimensional structure of every cluster was inspected to uncover differences in parenting between and within clusters. Third, the parenting styles were compared on several adolescent characteristics. Fourth, to examine the impact of change in parenting style over time, we looked at the cluster membership over time. Longitudinal questionnaire data were collected at three annual waves, with 1,116 adolescents (mean age = 13.79 years) at wave 1. Based on five parenting dimensions (support and proactive, punitive, psychological and harsh control), subspace K-means clustering, analysed per wave separately, identified two clusters (authoritative and authoritarian parenting) in which parenting dimensions were interrelated differently. Authoritative parenting seemed to be beneficial for adolescent development (less externalising problem behaviour and higher self-concept). Longitudinal data revealed several parenting group trajectories which showed differential relations with adolescent outcomes. Change in membership from the authoritative cluster to the authoritarian cluster was associated with a decrease in self-concept and an increase in externalising problem behaviour, whereas changes from the authoritarian cluster to the authoritative cluster were associated with an increase in self-concept and a decrease in externalising problem behaviour.

A SNP, Gene, and Polygenic Risk Score Approach of Oxytocin-Vasopressin Genes in Adolescents' Loneliness.

Not much is known regarding underlying biological pathways to adolescents' loneliness. Insight in underlying molecular mechanisms could inform intervention efforts aimed at reducing loneliness. Using latent growth curve modeling, baseline levels and development of loneliness were studied in two longitudinal adolescent samples. Genes (OXTR, OXT, AVPR1A, AVPR1B) were examined using SNP-based, gene-based, and polygenic risk score (PRS) approaches. In both samples, SNP- and gene-based tests showed involvement of the OXTR gene in development of loneliness, though, significance levels did not survive correction for multiple testing. The PRS approach provided no evidence for relations with loneliness. We recommend alternative phenotyping methods, including environmental factors, to consider epigenetic studies, and to examine possible endophenotypes in relation to adolescents' loneliness.

Perspective taking, empathic concern, agreeableness, and parental support: Transactional associations across adolescence.

INTRODUCTION: Empathy consists of a cognitive and an affective component, of which it is thought that there are gender differences. Previous studies also suggest that maternal and paternal support play a more prominent role in the development of an adolescent's affective and cognitive empathy, respectively. Besides the environmental factor, that is parenting, adolescent personality, and more specifically, agreeableness, is closely linked to both empathy and support, but this interplay was not extensively investigated longitudinally. The present study investigated the transactional associations among parental support, adolescent agreeableness, and adolescent empathy. More specifically, we examined (a) whether maternal/paternal support is differentially associated with cognitive/affective empathy, while taking into account adolescent agreeableness and (b) whether adolescent agreeableness still predicts empathy, while taking into account parental support. METHODS: Data from 993 Belgian adolescents (MageT1 = 13.96 years; [12.6-18.4]) and their parents across four time points were used in a random intercept cross-lagged panel model. RESULTS: At the between-person level, maternal support was associated with affective, but not cognitive empathy, whereas agreeableness was associated with maternal and paternal support as well as with both types of empathy. At the within-person level, affective empathy predicted cognitive empathy one wave later. CONCLUSIONS: At a population level, agreeableness and support are both important in adolescent empathy development with limited evidence for the differential roles of mothers and fathers. Within participants, affective empathy, and not parental support or agreeableness, predicted cognitive empathy.

Loneliness, Social Anxiety Symptoms, and Depressive Symptoms in Adolescence: Longitudinal Distinctiveness and Correlated Change.

Loneliness, social anxiety symptoms, and depressive symptoms are internalizing problems that are highly intertwined and often co-occur during adolescence. This overlap and co-occurrence raises the question whether three different labels are used for the same underlying phenomenon. The present study adopts a comprehensive approach to this issue by investigating the development of the three phenomena simultaneously. Specifically, this study aimed to investigate (1) the developmental trend for all three internalizing problems separately, (2) whether they are best described by a single developmental trend, (3) how they co-develop across adolescence, and (4) gender differences in this co-development. The analyses were run in three three-wave longitudinal samples of adolescents with one-year intervals in order to verify the robustness of the findings. Sample 1 (roughly ages 15, 16, and 17) comprised 549 adolescents (63% girls), and Samples 2 and 3 (roughly ages 13, 14, and 15) comprised 811 adolescents (46% girls) and 1101 adolescents (52% girls), respectively. Latent growth curve modeling for the three phenomena separately showed either small increases or stable patterns. A comparison of a Multiple Indicator Latent Growth Model (MILGM) with a Parallel Process Latent Growth Curve Modeling (PPLGCM) showed that the three internalizing problems followed unique, but related, developmental trends across adolescence. The intercepts of the three phenomena were positively correlated with one another in all samples and increases in loneliness were associated consistently with increases in social anxiety symptoms. Only in Sample 3 evidence was found for a similar association between loneliness and depressive symptoms and between social anxiety symptoms and depressive symptoms. Except for differences in initial levels, gender differences in the development of the three problems were limited. Overall, the results of the present study clearly indicate that the three internalizing problems are longitudinally distinct from one another, but co-develop across adolescence.

Manifesto for new directions in developmental science.

Although developmental science has always been evolving, these times of fast-paced and profound social and scientific changes easily lead to disorienting fragmentation rather than coherent scientific advances. What directions should developmental science pursue to meaningfully address real-world problems that impact human development throughout the lifespan? What conceptual or policy shifts are needed to steer the field in these directions? The present manifesto is proposed by a group of scholars from various disciplines and perspectives within developmental science to spark conversations and action plans in response to these questions. After highlighting four critical content domains that merit concentrated and often urgent research efforts, two issues regarding "how" we do developmental science and "what for" are outlined. This manifesto concludes with five proposals, calling for integrative, inclusive, transdisciplinary, transparent, and actionable developmental science. Specific recommendations, prospects, pitfalls, and challenges to reach this goal are discussed.

Developmental Change in Loneliness and Attitudes Toward Aloneness in Adolescence.

Adolescents are particularly vulnerable to experiencing feelings of loneliness. Changes in different social contexts and the inability to cope with these changes can result in different types of loneliness. According to the multidimensional view on loneliness, loneliness can be experienced in relationships with peers and parents and can be placed in a broader perspective by taking into account attitudes toward aloneness (i.e., positive and negative). However, we do not yet know how loneliness and attitudes toward aloneness develop across adolescence. These developmental trends were examined in two samples of Flemish adolescents consisting of 834 adolescents (61.9% girls, M age = 14.84; Sample 1), and 968 adolescents (58.6% girls, M age = 14.82; Sample 2), respectively. Adolescents filled out the Loneliness and Aloneness Scale for Children and Adolescents (LACA) during regular school hours on three (Sample 1) and four (Sample 2) measurement occasions with a 1-year interval. Latent growth curve modeling (LGCM) was applied. In line with theoretical notions, adolescents' parent-related loneliness and positive attitude toward aloneness were expected to increase, and adolescents' peer-related loneliness and negative attitude toward aloneness were expected to decrease. Clear evidence was found for the hypotheses regarding attitudes toward aloneness. The results regarding peer-related loneliness were inconsistent across samples and parent-related loneliness decreased, which was in contrast with theoretical expectations. In general, the two types of loneliness and attitudes toward aloneness changed in different directions during adolescence, suggesting the added value of a multidimensional view on loneliness.

Association between Non-Suicidal Self-Injury, Parents and Peers Related Loneliness, and Attitude Towards Aloneness in Flemish Adolescents: An Empirical Note.

Loneliness and attitude towards aloneness have been shown to be associated to depression, anxiety, and other psychiatric disorders in adolescents and they may also increase the vulnerability to Non-Suicidal Self-Injury (NSSI). Therefore, the present study investigated the association between lifetime prevalence and functions of NSSI, parent- and peer-related loneliness, and attitude towards aloneness (positive and negative). Data regarding NSSI, loneliness, and attitude towards aloneness were collected from a sample of 401 high school students from three different high schools located in the Dutch-speaking part of Belgium. Lifetime prevalence of NSSI was found to be 16.5%. Females reported a higher lifetime prevalence of NSSI than males. Higher mean scores for parent-, peer-related loneliness, and positive attitude (i.e., affinity) towards aloneness was observed in adolescents with lifetime NSSI as compared to adolescents without a history of NSSI. Finally, a positive correlation between self-related (i.e., automatic) functions of NSSI and parent- and peer-related loneliness and a positive attitude towards aloneness was also observed.

Gene-based interaction analysis shows GABAergic genes interacting with parenting in adolescent depressive symptoms.

BACKGROUND: Most gene-environment interaction studies (G × E) have focused on single candidate genes. This approach is criticized for its expectations of large effect sizes and occurrence of spurious results. We describe an approach that accounts for the polygenic nature of most psychiatric phenotypes and reduces the risk of false-positive findings. We apply this method focusing on the role of perceived parental support, psychological control, and harsh punishment in depressive symptoms in adolescence. METHODS: Analyses were conducted on 982 adolescents of Caucasian origin (Mage (SD) = 13.78 (.94) years) genotyped for 4,947 SNPs in 263 genes, selected based on a literature survey. The Leuven Adolescent Perceived Parenting Scale (LAPPS) and the Parental Behavior Scale (PBS) were used to assess perceived parental psychological control, harsh punishment, and support. The Center for Epidemiologic Studies Depression Scale (CES-D) was the outcome. We used gene-based testing taking into account linkage disequilibrium to identify genes containing SNPs exhibiting an interaction with environmental factors yielding a p-value per single gene. Significant results at the corrected p-value of p < 1.90 × 10-4 were examined in an independent replication sample of Dutch adolescents (N = 1354). RESULTS: Two genes showed evidence for interaction with perceived support: GABRR1 (p = 4.62 × 10-5 ) and GABRR2 (p = 9.05 × 10-6 ). No genes interacted significantly with psychological control or harsh punishment. Gene-based analysis was unable to confirm the interaction of GABRR1 or GABRR2 with support in the replication sample. However, for GABRR2, but not GABRR1, the correlation of the estimates between the two datasets was significant (r (46) = .32; p = .027) and a gene-based analysis of the combined datasets supported GABRR2 × support interaction (p = 1.63 × 10-4 ). CONCLUSIONS: We present a gene-based method for gene-environment interactions in a polygenic context and show that genes interact differently with particular aspects of parenting. This accentuates the importance of polygenic approaches and the need to accurately assess environmental exposure in G × E.

Loneliness in Children and Adolescents With Chronic Physical Conditions: A Meta-Analysis.

Objective: This meta-analysis examines loneliness in children and adolescents with chronic physical conditions as compared with their peers. Multilevel meta-analyses were performed on 43 studies (69 samples), published between 1987 and 2015. A total of 2,518 individuals with chronic physical conditions and 1,463 control peers were included in the analyses. Children and adolescents with chronic conditions are, on average, somewhat lonelier than their peers without such conditions. Moreover, the link between chronic conditions and loneliness varied according to the recruitment procedure used for participant selection. Stronger links were found for studies that recruited from patient organizations as compared with clinical registers. Findings support the link between loneliness and chronic conditions. To take into account the heterogeneity within patient groups, we advocate an alternative approach that cuts across diagnostic boundaries and focuses on illness-related variables such as illness duration and visibility of the condition.

Loneliness in Early Adolescence: Friendship Quantity, Friendship Quality, and Dyadic Processes.

Friendship quantity and quality are related to adolescent loneliness, but the exact link between these constructs is not well understood. The present study aimed to examine whether adolescents' perception of friendship quantity and quality, and the perceptions of their peers, were related to loneliness. We examined the relation between loneliness and the number of unilateral and reciprocal friendships and compared the views of best friendship quality. Overall, 1,172 Dutch adolescents (49.1% male, M age = 12.81, SD = .43) nominated their friends and rated their friendship quality. Friendship quantity was measured using sociometrics to distinguish reciprocated and unilateral (i.e., one-sided) friendships. The analyses indicated that loneliness was related to fewer reciprocal and unilateral-received friendships (i.e., the adolescent received a friendship nomination but did not reciprocate that nomination) and a lower quality of best friendship. Actor-partner interdependence analyses revealed that adolescents' loneliness was related to a less positive evaluation of their friendship, as reported by adolescents themselves (i.e., a significant actor effect) but not by their friends (i.e., nonsignificant partner effect). These findings (a) indicate that loneliness is negatively related to the number of friends adolescents have, as perceived by themselves and their peers and (b) suggest that, once a friendship is established, lonely adolescents may interpret the friendship quality less positively compared to their friends. Implications of these findings for our current understanding of adolescent loneliness are discussed, and suggestions for future research are outlined.

Externalizing Problem Behavior in Adolescence: Parenting Interacting With DAT1 and DRD4 Genes.

This study extends previous gene-by-environment (G × E) research through design and methodological advances and examines alternative hypotheses of diathesis stress, vantage sensitivity, and differential susceptibility. In a sample of 984 adolescents and their parents, we examined whether effects of parental support, proactive, punitive, harsh punitive, and psychological control on externalizing problem behavior are moderated by adolescents' genotype for the dopamine transporter (DAT1) or receptor D4 (DRD4) gene. Results provided evidence for main effects of parenting behavior and DRD4, and multiple interaction effects of which one survived Bonferroni correction. Adolescents carrying a long DRD4 variant were more susceptible to the effects of parental proactive control on aggression, for better and for worse. Critical considerations were made regarding the complexity of G × E research.

Parent-Adolescent Discrepancies Regarding Adolescents' Peer-Related Loneliness: Associations with Adolescent Adjustment.

Because loneliness is a subjective experience, it is often examined using self-reports. Yet, researchers have started to use other-reports to examine loneliness. As previous research suggests that discrepancies between self- and other views might have important implications for adolescents' mental health, the current study examines discrepancies in multi-informant reports on adolescents' loneliness in relation with prosocial behavior, aggression, and adolescents' parent-related loneliness. The sample consisted of 374 mother-adolescent dyads and 318 father-adolescent dyads (41.80% male, M age = 15.67 years, SD = 1.25). Results indicated that informants used different reference points to assess adolescents' peer-related loneliness, but were otherwise comparable. Moreover, informant discrepancies were associated with greater adolescents' reported parent-related loneliness. The current study did not provide evidence that discrepancies were related to prosocial or aggressive behavior. The current study adds to the notion that other-reports on loneliness show substantial convergence with self-reports. In addition, this study indicates that the discrepancy between other- and self-reports on loneliness holds valuable information for adolescent socio-emotional adjustment.

Adolescents' Loneliness and Depression Associated with Friendship Experiences and Well-Being: A Person-Centered Approach.

Loneliness and depressive symptoms are distinct, but partly overlapping constructs. The current study examined whether clusters of loneliness and depressive symptoms could be identified through latent profile analysis in two samples of 417 and 1140 adolescents (48.40 and 48.68 % male, respectively), on average 12.47 and 12.81 years old, respectively. Four clusters were identified, (1) low on loneliness and depressive symptoms, (2) low on loneliness and high on depressive symptoms, (3) high on loneliness and low on depressive symptoms, and (4) high on loneliness and depressive symptoms. We found that these four clusters were differentially related to friendship quantity and quality as well as to happiness and self-esteem. The current study stresses the importance of assessing both loneliness and depressive symptoms, as their mutual relation within individuals is differentially related to various aspects of adjustment.

Brief report: Factor structure of parenting behaviour in early adolescence.

Researchers have traditionally relied on a tripartite model of parenting behaviour, consisting of the dimensions parental support, psychological control, and behavioural control. However, some scholars have argued to distinguish two dimensions of behavioural control, namely reactive control and proactive control. In line with earlier work, the current study found empirical evidence for these distinct behavioural control dimensions. In addition, the study showed that the four parenting dimensions of parental support, psychological control, reactive control, and proactive control were differentially related to peer-related loneliness as well as parent-related loneliness. Thereby, the current study does not only provide empirical evidence for the distinction between various parenting dimensions, but also shows the utility of this differentiation.

Loneliness and Attitudes Toward Aloneness in Adolescence: A Person-Centered Approach.

In adolescence, feeling lonely and dealing with time spent alone become particularly salient. The present study examined the co-occurrence of parent- and peer-related loneliness, and positive and negative attitudes toward aloneness, using cluster analysis. In three independent samples, covering about 1800 adolescents (61 % female), six meaningful groups were identified. These groups showed different associations with adolescents' self-esteem and personality, parental responsiveness and psychological control, and peer group functioning and friendships. An adaptive pattern of correlates was found for adolescents in three of the six groups, that is, the Indifference group (with rather low scores on the four constructs), the Moderate group (with moderate scores on the four constructs), and the Negative Attitude Toward Aloneness group. A rather maladaptive pattern of correlates was found for adolescents in the three other groups, that is, the Peer-Related Loneliness group, the Positive Attitude Toward Aloneness group, and the Parent-Related Loneliness group. More specifically, adolescents in the Peer-Related Loneliness and Positive Attitude Toward Aloneness groups may need assistance regarding their relations with their peers, whereas adolescents in the Parent-Related Loneliness group may need assistance regarding their relations with their parents. Implications of these findings for current understanding and optimal measurement of adolescents' loneliness and aloneness are discussed.

Transactional Links Between Teacher-Student Relationships and Adolescent Rule-Breaking Behavior and Behavioral School Engagement: Moderating Role of a Dopaminergic Genetic Profile Score.

Throughout adolescence, there is an increase in rule-breaking behavior and a decrease in behavioral school engagement. The role of teacher-student relationship quality in the development of these adjustment problems remains understudied. This study examined how adolescent-reported teacher-student affiliation and dissatisfaction and parent-reported rule-breaking behavior and behavioral engagement impact one another throughout adolescence. In addition, we examined the moderating effect of genes by means of a Biologically Informed Multilocus genetic Profile Score (BIMPS), a composite score reflecting the cumulative effect of multiple dopaminergic genes, with a higher score indicating higher dopamine signaling in the adolescent brain. We used three-year longitudinal data from 1111 adolescents (51 % boys; M age = 13.79), and their parents. Cross-lagged analyses revealed a transactional process in which adolescents who display more rule-breaking behavior and less behavioral engagement experienced increased subsequent dissatisfaction with their teachers, which in turn further increased their adjustment problems. Also, adolescents with more adjustment problems experienced decreased subsequent affiliation with their teachers. The other way around, adolescents' behavioral engagement also benefitted from positive relationships with teachers. Multi-group analyses revealed genetic moderation for behavioral engagement, but not for rule-breaking. Specifically, adolescents who had a BIMPS score coding for moderate levels of dopamine signaling (instead of high or low signaling) were most affected in their behavioral engagement when they experienced dissatisfaction with their teachers. Our study findings may guide schools in implementing interventions to create a supportive class and school environment including positive, supportive teacher-student relationships and indicate that providing a such a supportive school environment is important for all adolescents.