[Effects of chemical constituent in roots of Achyranthes Bidentata].

The difference of ingredients between the roots from the SP1 plants of Achyranthes Bidentata carried by satellite and the control's were evaluated in this study. The techniques of ultraviolet-visible spectroscopy (UVS), infrared spectroscopy (IR), Xray diffraction (XRD) and SDS-PAGE were used to analyse the chemical constituent in the root of A. bidentata. The results demonstrated that the UVS, IR, XRD and protein fingerprints of the roots from A. bidentata were distinct with special characters. The difference of the IR, XRD and protein fingerprints could be discriminated the satellite plants roots from those of the control, however, there were no difference of the UVS fingerprints between the satellite plants roots and the control. This indicated that the kinds of chemical ingredients were not different between the two groups, but the contents of some chemical ingredients deceased in SP1 plants of A. bidentata carried by satellite.

[Analysis of mannoligosaccharides by liquid chromatography-electrospray ionization mass spectrometry].

Oligosaccharide characterization has been of utmost interest in various areas such as medicine, biochemistry, and food chemistry. These biologically relevant molecules are ideally suited for mass spectrometric investigation, because of the capability of this technique in offering structure and relative molecular mass information. Therefore, liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) was applied to characterize the acetolysis of mannan from Saccharomyces cerevisiae. The electrospray using Na+ as adducts proved to be superior to the LC-MS for the determination of mannoligosaccharides. LC separation was accomplished by the use of NH2 column and the elution by acetonitrile-water (70:30, volume ratio). The results showed that mannoligosaccharides side chain consisted of mannose, mannobiose, mannotriose and mannotetraose. The method developed is accurate, fast and convenient and can be used to characterize the relative molecular mass of the oligosaccharides.