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1.
Anal Chem ; 96(17): 6659-6665, 2024 04 30.
Artículo en Inglés | MEDLINE | ID: mdl-38635916

RESUMEN

The enhancement of sensitivity in biological analysis detection can reduce the probability of false positives of the biosensor. In this work, a novel self-on controlled-release electrochemiluminescence (CRE) biosensor was designed by multiple signal amplification and framework-enhanced stability strategies. As a result, the changes of the ECL signal were enhanced before and after the controlled-release process, achieving sensitive detection of prostate-specific antigen (PSA). Specifically, for one thing, Fe3O4@CeO2-NH2 with two paths for enhancing the generation of coreactant radicals was used as the coreaction accelerator to boost ECL performance. For another, due to the framework stability, zeolitic imidazolate framework-8-NH2 (ZIF-8-NH2) was combined with luminol to make the ECL signal more stable. Based on these strategies, the constructed CRE biosensor showed a strong self-on effect in the presence of PSA and high sensitivity in a series of tests. The detection range and limit of detection (LOD) were 5 fg/mL to 10 ng/mL and 2.8 fg/mL (S/N = 3), respectively, providing a feasible approach for clinical detection of PSA.


Asunto(s)
Técnicas Biosensibles , Técnicas Electroquímicas , Mediciones Luminiscentes , Antígeno Prostático Específico , Antígeno Prostático Específico/análisis , Antígeno Prostático Específico/sangre , Técnicas Biosensibles/métodos , Técnicas Electroquímicas/métodos , Humanos , Límite de Detección , Masculino , Cerio/química , Luminol/química
2.
Scand J Clin Lab Invest ; 81(7): 579-584, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34581638

RESUMEN

OBJECTIVE: To detect the concentration of high-sensitivity cardiac troponin T (hs-cTnT) in healthy children aged 0-14 years by electrochemiluminescence immunoassay (ECLIA), so as to explore the differences in different ages and genders. The aim of this study is to establish the reference interval for hs-cTnT in healthy children aged 0-14 years. METHODS: After screening, 3463 healthy children, including 1924 boys and 1539 girls, were selected from 4617 children aged 0-14 years. They were divided into nine groups: one day (umbilical cord blood; 'UCB'), one day (venous blood; 'VB'), 2-28 days, 29 days-<3 months, 3-<6 months, 6 months-<1 year old, 1-< 3 years old, 3-< 6 years old and 6-14 years old. A nonparametric test was used to detect the hs-cTnT concentration. The upper limit of the reference interval is the mean of the 99th percentile after bootstrap sampling. RESULTS: Hs-cTnT levels conformed to a non-Gaussian distribution. There was no significant difference in the concentration of hs-cTnT between boys and girls in the general data, but there were differences between boys and girls in the 3-<6 years old and 6-14 years old age groups. Except for UCB and 2-28 days, the concentration of hs-cTnT was significantly different in other age groups. The level of hs-cTnT in neonatal serum (2-28 days) was the highest. In other groups, it decreased gradually with age and dropped to the reference range of adults (0-14ng/L) at one-year old. The upper limit of reference interval of hs-cTnT concentration in each group was, respectively, 60.8, 78.8, 96.6, 58.6, 34.2, 16.2, 11.4, 8.0 (7.8 female), and 7.9 (7.3 female) ng/L. CONCLUSIONS: Referring to WS/T 402-2012 establishment of reference intervals for clinical laboratory testing projects and CLSI (Clinical and Laboratory Standards Institute) C28-A3 documents and the joint expert consensus of ESC (European Society of Cardiology) and ACC (American College of Cardiology) in 2007, we established the reference interval of hs-cTnT concentration in children aged 0-14 years in Chongqing Nan'an district of China which can provide certain reference value for clinical diagnosis and treatment of myocarditis and myocardial (micro) injury in children.


Asunto(s)
Miocardio/metabolismo , Troponina T/sangre , Adolescente , Niño , Preescolar , China , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Valores de Referencia , Estadísticas no Paramétricas
3.
Anal Biochem ; 457: 59-64, 2014 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-24769071

RESUMEN

In this study, a novel sensitive electrochemiluminescence (ECL) immunosensor was constructed by carboxyl graphene (GR) for enhancing luminol-O2 system emission. Here, carboxyl GR was used to enhance the ECL intensity of luminol that had excellent electron transfer ability and good solubility. The sensing platform was constructed by depositing carboxyl GR on electrodes and immobilizing antibodies on the surface of carboxyl GR through amidation. The specific immunoreaction between α-fetoprotein (AFP) and antibodies resulted in a decrease of ECL intensity, and the intensity decreased linearly with AFP concentrations in the range of 5 pg ml(-1) to 14 ng ml(-1) with a detection limit of 2.0 pg ml(-1). The proposed immunosensor exhibits high specificity, good reproducibility, and longtime stability. It may become a promising technique for protein detection.


Asunto(s)
Técnicas Electroquímicas/métodos , Grafito/química , Luminiscencia , Luminol/química , alfa-Fetoproteínas/química , Animales , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/inmunología , Biomarcadores de Tumor/análisis , Técnicas Biosensibles/métodos , Bovinos , Electrodos , Humanos , Proteínas Inmovilizadas/química , Proteínas Inmovilizadas/inmunología , Mediciones Luminiscentes/instrumentación , Mediciones Luminiscentes/métodos , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Albúmina Sérica Bovina
4.
J Neurosci Methods ; 406: 110115, 2024 06.
Artículo en Inglés | MEDLINE | ID: mdl-38531478

RESUMEN

BACKGROUND: Parkinson's disease (PD) is a disorder with abnormal changes in brain activity. The lack of objective indicators makes the assessment of PD progression difficult. Assessment of brain activity changes in PD may offer a potential solution. NEW METHOD: Electroencephalogram (EEG) microstates reflect global dynamic changes in the brain. Therefore, we utilized microstates to assess changes in PD brain activity. However, the effect of epoch duration on the reliability of microstate analyses in PD is unclear. Thus, we first assessed the effect of data duration on the reliability of microstate topography and temporal features in PD and older healthy individuals. According to the reliability assessment, EEG epochs with high reliability were selected for microstate analysis in PD. Finally, we investigated the correlation between microstate features and clinical scales to determine whether these features could serve as objective indicators to evaluate PD progression. RESULTS: Microstate analysis features that show high reliability for 3 min and above epoch durations. The topology of microstate D was significantly changed in PD compared to healthy controls, as well as the temporal features of microstates C and D. Additionally, the occurrence of C was negatively correlated with MoCA, and the duration of D was positively correlated with UPDRS. COMPARISON WITH EXISTING METHOD(S): High reliability of PD microstate features obtained by our approach. CONCLUSION: EEG for PD microstate analysis should be at least 3 min. Microstate analysis is expected to provide new ideas and objective indicators for assessing Parkinson's disease progression in the clinical setting.


Asunto(s)
Electroencefalografía , Enfermedad de Parkinson , Enfermedad de Parkinson/fisiopatología , Enfermedad de Parkinson/diagnóstico , Humanos , Masculino , Anciano , Reproducibilidad de los Resultados , Electroencefalografía/métodos , Femenino , Persona de Mediana Edad , Encéfalo/fisiopatología , Progresión de la Enfermedad
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