Formaldehyde Adsorption-Desorption of Poplar Bark.

This study investigated how the Populus × euramericana cv. Pannónia bark behaves in an environment containing formaldehyde. Prism shaped samples were formed from the bark and the prisms were kept in formaldehyde contaminated atmosphere for 1, 2, 5, 10, 18 and 36 days. After the contamination, the amount of the formaldehyde adsorbed and later the desorbed was measured. The formaldehyde content of the uncontaminated poplar bark was 0.0036 mg/g. The amount of bound formaldehyde showed a saturation curve as a function of time. The formaldehyde adsorption reached an equilibrium value of 0.9 mg HCHO/g bark. The emission of formaldehyde from contaminated bark samples showed an exponential curve as a function of time and some residual formaldehyde content was detected after the formaldehyde was released.

Interferon-gamma-release assay prevents unnecessary tuberculosis therapy.

BACKGROUND: The mass influx of immigrants from tuberculosis-endemic countries into Israel was followed by a considerable increase in the incidence of tuberculosis (TB). All contacts of active TB patients are obliged to be screened by tuberculin skin tests (TST) and, if found positive, prophylactic treatment is considered. OBJECTIVES: To assess the utility of interferon-gamma (IFNγ)-release assay with a prolonged follow-up in preventing unnecessary anti-TB therapy in individuals with suspected false positive results. METHODS: Between 2008 and 2012 the QuantiFERON TB gold-in-tube test (QFT-G) was performed in 278 sequential individuals who were mostly TST-positive and/or were in contact with an active TB patient. In all, whole blood was examined by the IFNγ-release assay. We correlated the TST diameter with the QFT-G assay and followed those patients with a negative assay. RESULTS: The QFT-G test was positive in only 72 (42%) of all 171 TST-positive individuals. There was no correlation between the diameter of TST and QFT-G positivity. Follow-up over 5 years was available in 128 (62%) of all QFT-G-negative individuals. All remained well and none developed active TB. CONCLUSIONS: A negative QFT-G test may obviate the need for anti-TB therapy in more than half of those with a positive TST.

Valorization of Larix decidua Mill. bark by functionalizing bioextract onto chitosan films for sustainable active food packaging.

The present study explored the use of chitosan films functionalized with antioxidants extracted from Larix decidua Mill. bark for active packaging. The pristine chitosan and extract-incorporated chitosan films were evaluated for their structural, physico-mechanical, thermal, viscoelastic and antioxidant properties using advanced characterization techniques. The infrared spectroscopy revealed hydrogen bonding between the extract polyphenolic antioxidants and chitosan, whereas the surface microscopy studies indicated good compatibility between them. The addition of bark extract caused a significant increase in color parameters and solubility with reduction in swelling and elongation at break of the films. The thermal analysis indicated a drop in thermal stability of chitosan films modified with the extract. The dynamic mechanical analysis confirmed the extract-polymer interactions and the viscoelastic nature of the films. The incorporation of bark extract caused remarkable enhancement in the antioxidant activity of chitosan films. Overall, larch bark extract-functionalized chitosan films demonstrated promising potential for food packaging.

Properties of ultrasound extracted bicomponent lignocellulose thin films.

Agricultural and forest residues obtained after harvesting are promising renewable sources, suitable as a source of pulp for cellulose nanocrystal manufacturing. Cavitation-assisted softening of the lignin-carbohydrate matrix offers sample opportunity for cellulose fibril liberation and degradation of amorphous cellulose. The present work addresses cavitation assisted cellulose fibril and crystal liberation and film forming properties of the supernatant phase of treated agricultural and forest residues. The effectiveness of this method has been evaluated according to crystallinity indices and hydrogen bond energies, as measured by FT-IR analysis. It has been observed that the use of cavitation increased the crystallinity and caused partial removal and degradation of the lignin matrix. Overall, it appears that considerable improvement of crystallinity can be obtained from agricultural and forest residues through the use of cavitation.

ZnO-modified cellulose fiber sheets for antibody immobilization.

Cellulose fiber sheets impregnated with saccharide capped-ZnO nanoparticles were used as bioactive materials for antibody immobilization. First, ZnO nanoparticles were synthesized in the presence of glucose (monosaccharide), sucrose (disaccharide) as well as alginic acid and starch (polysaccharides). The pine cellulose fibers were then modified by the obtained saccharide capped nanoparticles and further incorporated into the sheets. The presence of ZnO significantly improved the immobilization of the antibodies on the surface of the sheets. After rewetting the alginic acid-ZnO modified sheets with saline solution, the retention of antibodies was about 95%. A high degree of the immobilization of biomolecules is an important feature for possible fabrications of bioactive- or biosensing-papers and we successfully tested the sheets on the detection of blood types using (A, B, and D blood antibodies). The ZnO nanoparticles affected also the other properties of the sheets. The ZnO-modified fiber sheets showed higher values of tensile index (strength), smoothness and opacity, while the value of porosity was substantially lower than that of the unmodified sheet. The presence of ZnO nanoparticles provided also the antimicrobial activity to the sheets. They showed a strong activity against bacteria (Escherichia coli and Staphylococcus aureus) and strong resistance to the attack of cellulase producing fungus Gloeophyllum trabeum.

Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus.

INTRODUCTION: Semaphorin 3A (sema3A) and neuropilin-1 (NP-1) play a regulatory role in immune responses and have a demonstrated effect on the course of collagen induced arthritis. This study was undertaken to evaluate the role of sema3A and NP-1 in the pathogenesis of systemic lupus erythematosus (SLE) and the specific effect of sema3A on the auto-reactive properties of B cells in SLE patients. METHODS: Thirty two SLE and 24 rheumatoid arthritis (RA) patients were assessed and compared with 40 normal individuals. Sema3A serum levels were measured and correlated with SLE disease activity. The in vitro effect of sema3A in reducing Toll-like receptor 9 (TLR-9) expression in B cells of SLE patients was evaluated. RESULTS: Sema3A serum levels in SLE patients were found to be significantly lower than in RA patients (55.04 ± 16.30 ng/ml versus 65.54 ± 14.82 ng/ml, P = 0.018) and lower yet than in normal individuals (55.04 ± 16.30 ng/ml versus 74.41 ± 17.60 ng/ml, P < 0.0001). Altered serum sema3A levels were found to be in inverse correlation with SLE disease activity, mainly with renal damage. The expression of both sema3A and NP-1 on B cells from SLE patients was significantly different in comparison with normal healthy individuals. Finally, when sema3A was co-cultured with cytosine-phosphodiester-guanine oligodeoxynucleotides (CpG-ODN)-stimulated B cells of SLE patients, their TLR-9 expression was significantly reduced, by almost 50% (P = 0.001). CONCLUSIONS: This is the first study in which a reduced serum level of sema3A was found in association with SLE disease activity. It also raises the possibility that sema3A may have a regulatory function in SLE.