RESUMEN
Aquaporin 2 (AQP2), AQP5 and AQP8 participate in adenomyosis (AM). Ηowever, the roles of these three molecules in AM have not been fully elucidated. In the present study, Institute of Cancer Research female mice were used to establish a model of AM. Subsequently, the endometrial tissues of the mice were observed by hematoxylineosin staining, and AM severity, uterus diameter, uterus index, ovary index and numbers of nodules on the uterine surface were evaluated and counted. In addition, eutopic and ectopic endometrial stromal cells (ESCs) were isolated from eutopic and ectopic endometrial samples derived from patients with AM and were then identified by immunofluorescence. The viability, and migratory and invasive ability of ESCs transfected with small interfering RNA targeting AQP5 (siAQP5) were determined by Cell Counting Κit8, scratch woundhealing and Transwell assays, respectively. Reverse transcriptionquantitative polymerase chain reaction was performed to determine the mRNA expression levels of AQP5, epithelialmesenchymal transition (EMT)related genes (Eand Ncadherin), matrix metalloproteinase (MMP)2 and 9. Protein expression levels of AQP2, AQP5, AQP8, E, Ncadherin, MMP2 and 9 were detected by western blotting. AM severity and uterus index were higher, and there were a greater number of nodules on the uterine surface in the AM group compared with the sham group. AQP2, AQP5 and AQP8 proteins were highly expressed in eutopic and ectopic endometrium of the AM group, and AQP5 was more highly expressed than AQP2 or AQP8. In addition, the data showed that Vimentin was positively expressed in ESCs, and that siAQP5 suppressed the mRNA expression levels of AQP5, cell viability, migration, invasion, EMT and MMP2 and 9 expression in ESCs. In conclusion, AQP2, AQP5 and AQP8 were highly expressed in eutopic and ectopic endometrium. Notably, AQP5 silencing may suppress AM by inhibiting viability, migration, invasion, EMT, and MMP2 and 9 expression in ESCs.
Asunto(s)
Adenomiosis/genética , Acuaporina 2/genética , Acuaporina 5/genética , Acuaporinas/genética , Regulación hacia Arriba , Adenomiosis/etiología , Adenomiosis/metabolismo , Adulto , Animales , Acuaporina 2/metabolismo , Acuaporina 5/metabolismo , Acuaporinas/metabolismo , Movimiento Celular , Proliferación Celular , Supervivencia Celular , Células Cultivadas , Modelos Animales de Enfermedad , Transición Epitelial-Mesenquimal , Femenino , Regulación de la Expresión Génica , Humanos , Metaloproteinasa 2 de la Matriz/genética , Metaloproteinasa 2 de la Matriz/metabolismo , Metaloproteinasa 9 de la Matriz/genética , Metaloproteinasa 9 de la Matriz/metabolismo , Ratones , Persona de Mediana EdadRESUMEN
Uterine corpus endometrial carcinoma (UCEC) is one of the most common cancer in female worldwide. PIK3CA has been proven to be a strong prognostic biomarker in UCEC. Nevertheless, current studies have not investigated what effects PIK3CA had on tumor associated neutrophils (TANss). Kaplan-Meier methods were used to compute the survival time of TCGA UCEC patients. GO and KEGG enrichment analysis unveiled relevant pathways PIK3CA affected using DEGs between PIK3CA high expression group and PIK3CA low expression group in TCGA UCEC, as well as GSEA. immune infiltration status was calculated using TIMER. We found that PIK3CA influenced a number of pathways including immune related pathways. The fraction of TANs was certainly altered by PIK3CA expression in UCEC. Our findings suggest that PIK3CA expression may play an important role in tumor immune microenvironment and could alter fraction of TANs in UCEC.
Asunto(s)
Fosfatidilinositol 3-Quinasa Clase I/inmunología , Neoplasias Endometriales/inmunología , Neutrófilos/inmunología , Neoplasias Uterinas/inmunología , Bases de Datos Genéticas , Neoplasias Endometriales/genética , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Estimación de Kaplan-Meier , Neoplasias Uterinas/genéticaRESUMEN
Professor GAO Yuchun's experience is introduced in insomnia treated with the acupuncture technique for regulating the governor vessel and calming down the mind. GAO's acupuncture technique is in one of the series of character techniques in the acupuncture academic school in the region of Hebei province. Professor GAO believes that insomnia resultes from the disharmony of the nutrient and the defensive and the dysfunction of spleen and stomach. The acupoints are selected on the basis of regulating the governor vessel, calming down the mind, nou-rishing the water and the wood to harmonize the mind, strengthening the spleen and stomach to calm down the mind, in which the acupoints of the governor vessel and the yuan-primary points are used in combination. For regulating the governor vessel, Baihui (GV 20) and Shenting (GV 24) are used, combined with Zhongwan (CV 12) for regulating the nutrient and the defensive and calming down the mind. The yuan-primary points of the heart, liver and kidney meridians are selected to nourish water and wood and harmonize the mind. Tianshu (ST 25), Zhongwan (CV 12) and Yinlingquan (SP 9) are used to strengthen the spleen and stomach and for the treatment of the root cause of disease. Additionally, the sequence of needling, the needling techniques for reinforcing and reducing, as well as needle withdrawing are considered. The idea of regulating the governor vessel and calming down the mind as well as the needling techniques for insomnia are considerably introduced in the paper.