Effect of conventional and biodegradable microplastics on earthworms during vermicomposting process.

The potential effect of microplastics is an increasingly growing environmental issue. However, very little is known regarding the impact of microplastics on the vermicomposting process. The present study explored the effect of non-biodegradable (low density polyethylene; LDPE) and biodegradable (polybutylene succinate-co-adipate; PBSA) microplastics on earthworm Eisenia fetida during vermicomposting of cow dung. For this, earthworms were exposed to different concentrations (0, 0.5, 1 and 2%) of LDPE and PBSA of 2 mm size. The cow dung supported the growth and hatchlings of earthworms, and the toxicity effect of both LDPE and PBSA microplastics on Eisenia fetida was analyzed. Microplastics decreased the body weight of earthworms and there was no impact on hatchlings. The body weight of earthworm decreased from 0 to 60th day by 18.18% in 0.5% of LDPE treatment, 5.42% in 1% of LDPE, 20.58% in 2% of LDPE, 19.99% in 0.5% of PBSA, 15.09% in 1% of PBSA and 16.36% in 2% of PBSA. The physico-chemical parameters [pH (8.55-8.66), electrical conductivity (0.93-1.02 (S/m), organic matter (77.6-75.8%), total nitrogen (3.95-4.25 mg/kg) and total phosphorus (1.16-1.22 mg/kg)] do not show much significant changes with varying microplastics concentrations. Results of SEM and FTIR-ATR analysis observed the surface damage of earthworms, morphological and biochemical changes at higher concentrations of both LDPE and PBSA. The findings of the present study contribute to a better understanding of microplastics in vermicomposting system.

Psychological effects of meditation at a Buddhist monastery in Myanmar.

BACKGROUND: Meditation is widely used as a therapeutic measure because it can effectively reduce stress, pain, and improve the mental health, but there are many unclear points about the psychological effects of meditation. AIMS: The purpose of this study is to verify the psychological effects of meditation. METHOD: The short version of Profile of Mood States (POMS-SF) and the Acceptance and Action Questionnaire-II (AAQ-II) were conducted to compare 97 Myanmar people practicing Vipassana contemplation training with 81 Myanmar nurses who did not have any experience with meditation. RESULTS: The results of our study revealed that meditation mitigated depressive mood, anger, hostility, and fatigue and increased vigor. The enhancement of psychological flexibility occurred only after practicing meditation for more than a year. CONCLUSIONS: It can be considered that meditation mitigates anger, hostility and fatigue and increases vigor at a relatively early stage after starting meditation practice, and if meditation practice is continued for more than a year, enhancement of psychological flexibility can also be expected.

Effect of plastic mulch residue on plant growth performance and soil properties.

Plastic mulch is widely utilized for weed control, temperature regulation, soil erosion prevention, disease management, and soil structure improvement, ultimately enhancing crop quality and yield. However, a significant issue with conventional plastic mulches is their low recycling rates, which can cause plastic residue to build up, thereby damaging soil quality and reducing crop yield. The emergence of biodegradable films offers a promising solution to mitigate this issue and reduce soil pollution. However, its potential effects on soil properties and plant performance remain unclear. In this study, low-density polyethylene (LDPE) and poly (butylene succinate-co-butylene adipate) (PBSA) were used to observe the effect of plastic mulch residues on soil properties and plant growth performance via potting experiment. Additionally, the interaction effects of compost and biochar as soil amendments with plastic mulch residues were also evaluated. The result of this study revealed that the type of plastic significantly affected the total nitrogen and magnesium uptake; however, the morphological traits of the tested plant (Japanese mustard spinach) were not significantly affected. The addition of compost and biochar led to a significant increase in both shoot and total dry weight of the plant, indicating a positive effect on its growth. The results of the two-way ANOVA indicated a significant influence of plastic type on dissolved phosphate (PO43-) levels and soil dehydrogenase activity (DHA). The interaction effect (plastic type with soil amendment) was statistically significant only for soil DHA. Neither plastic mulch residues nor soil amendments significantly affected other soil chemical properties. However, long-term experiments to systematically investigate the long-term effects of plastic residues are necessary.

Distribution of extracellular and intracellular antibiotic resistance genes in sludge fractionated in terms of settleability.

The widespread proliferation of antibiotic resistance genes (ARGs) is a serious environmental and human health issue. Wastewater treatment facilities (WWTFs) are potential sources to spread ARGs to natural environment, for which, the presence state of ARGs in the sludge, as extracellular ones (eARGs) or intracellular ones (iARGs), along with the sludge settleability, are very important factors. The sludge settleability is closely associated with its floc size and density, bacterial activity, and the proportion of intact/damaged bacterial cells that aggregate together to form flocs for separation in the sedimentation process. It is reasonable to hypothesize that the distribution of eARGs and iARGs may differ with the sludge fractions of different settleability, a topic of great academic and practical significance requiring clarification. In this study, sludge samples from the aerobic contact tank of six household WWTFs were fractionated into fractions with different settling velocities: sludge of low settleability (LS), medium settleability (MS) and high settleability (HS); and the distribution of eARGs and iARGs in the obtained fractions for the widely detected tet G, tet M and sul 1 in water environment was evaluated based on the PMA-qPCR method, together with the evaluation for the well reported mobile genomic element intl 1 and total bacterial 16S rDNA. For the LS fractions, which contained more damaged bacterial cells, the distribution percentages of eARGs were generally higher than those of iARGs. For the HS fractions, which contained flocs with larger sizes formed by both intact and damaged bacterial cells, the relative abundances of ARGs and intl 1 were found apparently lower even if the presence percentages of eARGs were comparatively higher. It is thus inferable that sludge fractions of LS may possess higher transfer potential for ARGs and enhancing their settleability through optimization of the operation conditions is important for mitigating the proliferation of ARGs.