RESUMEN
The introduced work represents an implementation of the automatic benchtop electrochemical station (BES) as an effective tool for the possibilities of high-throughput preparation of modified sensor/biosensors, speeding up the development of the analytical method, and automation of the analytical procedure for the determination of paracetamol (PAR) and dopamine (DOP) as target analytes. Within the preparation of gold nanoparticles modified screen-printed carbon electrode (AuNPs-SPCE) by electrodeposition, the deposition potential EDEP, the deposition time tDEP, and the concentration of HAuCl4 were optimized and their influence was monitored on 1 mM [Ru(NH3)6]3+/2+ redox probe and 50 µM DOP. The morphology of the AuNPs-SPCE prepared at various modification conditions was observed by SEM. The analytical performance of the AuNPs-SPCE prepared at different modification conditions was evaluated by a construction of the calibration curves of DOP and PAR. SPCE and AuNPs-SPCE at modification condition providing the best sensitivity to PAR and DOP, were successfully used to determine PAR and DOP in tap water by "spike-recovery" approach. The BES yields better reproducibility of the preparation of AuNPs-SPCE (RSD = 3.0%) in comparison with the case when AuNPs-SPCE was prepared manually by highly skilled laboratory operator (RSD = 7.0%).
Asunto(s)
Acetaminofén , Dopamina , Técnicas Electroquímicas , Oro , Nanopartículas del Metal , Acetaminofén/análisis , Dopamina/análisis , Oro/química , Nanopartículas del Metal/química , Técnicas Electroquímicas/métodos , Técnicas Electroquímicas/instrumentación , Electrodos , Técnicas Biosensibles/métodos , Límite de Detección , Carbono/químicaRESUMEN
The purpose of the proposed review is to refer the contemporary capability of automated analytical systems, in particular batch injection analysis (BIA) in connection with electrochemical detection, for widespread applications in analytical chemistry. This combination recently represents an efficient tool for improvement of method parameters, such as speed, selectivity, and sampling rate for sensing of miscellaneous organic and inorganic substances. The review is focused on conception and usage of BIA in tandem with electrochemical detection utilizing various techniques, namely amperometry, voltammetry, and multiple pulse amperometry, as well as design of electrochemical cells constructed for BIA systems is discussed. Finally, this paper also summarizes the comprehensive overview of works published from 2015 to 2020 dealing with the electrochemical determination of different analytes by BIA in various matrices.