Metabolic regulation of homologous recombination repair by MRE11 lactylation.

Lactylation is a lactate-induced post-translational modification best known for its roles in epigenetic regulation. Herein, we demonstrate that MRE11, a crucial homologous recombination (HR) protein, is lactylated at K673 by the CBP acetyltransferase in response to DNA damage and dependent on ATM phosphorylation of the latter. MRE11 lactylation promotes its binding to DNA, facilitating DNA end resection and HR. Inhibition of CBP or LDH downregulated MRE11 lactylation, impaired HR, and enhanced chemosensitivity of tumor cells in patient-derived xenograft and organoid models. A cell-penetrating peptide that specifically blocks MRE11 lactylation inhibited HR and sensitized cancer cells to cisplatin and PARPi. These findings unveil lactylation as a key regulator of HR, providing fresh insights into the ways in which cellular metabolism is linked to DSB repair. They also imply that the Warburg effect can confer chemoresistance through enhancing HR and suggest a potential therapeutic strategy of targeting MRE11 lactylation to mitigate the effects.

Epithelial Nlrp10 inflammasome mediates protection against intestinal autoinflammation.

Unlike other nucleotide oligomerization domain-like receptors, Nlrp10 lacks a canonical leucine-rich repeat domain, suggesting that it is incapable of signal sensing and inflammasome formation. Here we show that mouse Nlrp10 is expressed in distal colonic intestinal epithelial cells (IECs) and modulated by the intestinal microbiome. In vitro, Nlrp10 forms an Apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC)-dependent, m-3M3FBS-activated, polyinosinic:polycytidylic acid-modulated inflammasome driving interleukin-1ß and interleukin-18 secretion. In vivo, Nlrp10 signaling is dispensable during steady state but becomes functional during autoinflammation in antagonizing mucosal damage. Importantly, whole-body or conditional IEC Nlrp10 depletion leads to reduced IEC caspase-1 activation, coupled with enhanced susceptibility to dextran sodium sulfate-induced colitis, mediated by altered inflammatory and healing programs. Collectively, understanding Nlrp10 inflammasome-dependent and independent activity, regulation and possible human relevance might facilitate the development of new innate immune anti-inflammatory interventions.

Lung dendritic-cell metabolism underlies susceptibility to viral infection in diabetes.

People with diabetes feature a life-risking susceptibility to respiratory viral infection, including influenza and SARS-CoV-2 (ref. 1), whose mechanism remains unknown. In acquired and genetic mouse models of diabetes, induced with an acute pulmonary viral infection, we demonstrate that hyperglycaemia leads to impaired costimulatory molecule expression, antigen transport and T cell priming in distinct lung dendritic cell (DC) subsets, driving a defective antiviral adaptive immune response, delayed viral clearance and enhanced mortality. Mechanistically, hyperglycaemia induces an altered metabolic DC circuitry characterized by increased glucose-to-acetyl-CoA shunting and downstream histone acetylation, leading to global chromatin alterations. These, in turn, drive impaired expression of key DC effectors including central antigen presentation-related genes. Either glucose-lowering treatment or pharmacological modulation of histone acetylation rescues DC function and antiviral immunity. Collectively, we highlight a hyperglycaemia-driven metabolic-immune axis orchestrating DC dysfunction during pulmonary viral infection and identify metabolic checkpoints that may be therapeutically exploited in mitigating exacerbated disease in infected diabetics.

Synthesis of Efficient S-Scheme Heterostructures Composed of BiPO4 and KNbO3 for Photocatalytic N2 Fixation and Water Purification.

The preparation of catalysts with heterojunction structures is a strategy to achieve efficient charge separation and high photocatalytic activity of photocatalysts. In this work, BiPO4/KNbO3 heterostructure photocatalysts were fabricated by a combination of hydrothermal and precipitation methods and subsequently employed in catalyzing N2-to-NH3 conversion and RhB degradation under light illumination. Morphological analysis revealed the effective dispersion of BiPO4 on KNbO3 nanocubes. Band structure analysis suggests that KNbO3 and BiPO4 exhibit suitable band potentials to form an S-scheme heterojunction. Under the joint action of the built-in electric field at the interface, energy band bending, and Coulomb attraction force, photogenerated electrons and holes with low redox performance are consumed, while those with high redox performance are effectively spatially separated. Consequently, the BiPO4/KNbO3 shows enhanced photocatalytic activity. The NH3 production rate of the optimal sample is 2.6 and 5.8 times higher than that of KNbO3 and BiPO4, respectively. The enhanced photoactivity of BiPO4/KNbO3 is also observed in the photocatalytic degradation of RhB. This study offers valuable insights for the design and preparation of S-scheme heterojunction photocatalysts.

Effect of the pipeline embolization device placement on branching vessels in anterior circulation: a systematic review and meta-analysis.

BACKGROUND AND PURPOSE: Pipeline embolization device (PED) is widely used in intracranial aneurysms, and the scope of applications for the PED, which is frequently used to treat cerebral aneurysms, is also growing. It has some effect on branching vessels as a result of its inherent properties. The effects of PED on the complications rate and branching vessels blockage have not yet been thoroughly investigated. OBJECTIVE: We conducted a systematic review searching reports from multiple databases on PED use for intracranial aneurysms, and analyzed the influence of PED on the occlusion rate of different branching vessels, and the influence of the amount of PED on the occlusion rate of branching vessels by meta-analysis. METHODS: We searched the literature using PUBMED, Web of Science, and OVID databases until August 2023. Inclusion criteria were that the study used only PED, included at least 10 patients, and recorded branching vessels occlusion rates, mortality, and neurological complications. RESULTS: Nine studies were analyzed consisting of 706 patients with 986 side branches. The results of the meta-analysis showed that application of more than one PED did not significantly elevate the rate of branching vessels occlusion compared to application of one PED (OR = 0.70; 95% CI: 0.34 to 1.43; P = 0.33). In the comparison of branching vessels occlusion rates in the anterior circulation, the anterior cerebral artery (ACA) had a significantly higher occlusion rate compared to the ophthalmic artery (OphA) (OR = 6.54; 95% CI: 3.05 to 14.01; P < 0.01), ACA also had a higher occlusion rate compared to the anterior choroidal artery (AchA) (OR = 15.44; 95% CI: 4.11 to 57.94 P < 0.01), ACA versus posterior communicating artery (PcomA) occlusion rate difference was not statistically significant (OR = 2.58; 95% CI: 0.63 to 12.82; P = 0.17), OphA versus AchA occlusion rate difference was not statistically significant (OR = 2.56; 95% CI: 0.89 to 7.38; P = 0.08), and the occlusion rate was significantly higher for PcomA compared to AchA (OR = 7.22; 95% CI: 2.49 to 20.95; P < 0.01) and lower for OphA compared to PcomA (OR = 0.33; 95% CI: 0.19 to 0.55; P < 0.01). CONCLUSION: The meta-analysis shows that use of multiple PEDs did not significantly increase the occlusion rate of branching vessels, and the larger the diameter of branching vessels covered by PED, the higher the occlusion rate of branching vessels. However, the incidence of complications is low after branching vessels occlusion in anterior circulation, which is related to the collateral circulation compensation of the branching vessels.

Wall-associated kinase GhWAK13 mediates arbuscular mycorrhizal symbiosis and Verticillium wilt resistance in cotton.

The cell wall is the major interface for arbuscular mycorrhizal (AM) symbiosis. However, the roles of cell wall proteins and cell wall synthesis in AM symbiosis remain unclear. We reported that a novel wall-associated kinase 13 (GhWAK13) positively regulates AM symbiosis and negatively regulates Verticillium wilt resistance in cotton. GhWAK13 transcription was induced by AM symbiosis and Verticillium dahliae (VD) infection. GhWAK13 is located in the plasma membrane and expressed in the arbuscule-containing cortical cells of mycorrhizal cotton roots. GhWAK13 silencing inhibited AM colonization and repressed gene expression of the mycorrhizal pathway. Moreover, GhWAK13 silencing improved Verticillium wilt resistance and triggered the expression of immunity genes. Therefore, GhWAK13 is considered an immune suppressor required for AM symbiosis and disease resistance. GhWAK7A, a positive regulator of Verticillium wilt resistance, was upregulated in GhWAK13-silenced cotton plants. Silencing GhWAK7A improved AM symbiosis. Oligogalacturonides application also suppressed AM symbiosis. Finally, GhWAK13 negatively affected the cellulose content by regulating the transcription of cellulose synthase genes. The results of this study suggest that immunity suppresses AM symbiosis in cotton. GhWAK13 affects AM symbiosis by suppressing immune responses.

In Situ Fabrication of an S-Scheme NaNbO3/Bi2O2CO3 Heterojunction for Enhanced Performance in Photocatalytic Nitrogen Fixation.

In this study, NaNbO3 microcubes were introduced during the preparation of Bi2O2CO3 nanosheets to construct a series of NaNbO3/Bi2O2CO3 heterojunctions with varying NaNbO3 content. Their photoactivities for N2 fixation were examined and compared. Results demonstrated that 7.5% NaNbO3/Bi2O2CO3 had the highest photoactivity. The NH3 production rate under simulated solar light is 453.1 µmol L-1 g-1 h-1, representing 2.0 and 3.8-fold increases compared to those of Bi2O2CO3 and NaNbO3, respectively. A comprehensive investigation encompassing the physical and chemical properties of the NaNbO3/Bi2O2CO3 photocatalyst was conducted. Bi2O2CO3 nanosheets were discovered to be distributed on the NaNbO3 microcubes surface. The addition of NaNbO3 exhibited nearly no effect on the photoabsorption performance and specific surface area of the Bi2O2CO3. However, the tight contact between NaNbO3 and Bi2O2CO3 and their appropriate band positions led to the formation of a heterojunction structure between them. The electron drift occurring in the interface region induces the creation of an internal electric field and energy band bending. This facilitates the transfer of photogenerated electrons and holes through an S-scheme mechanism, achieving efficient separation without compromising the redox performance. As a result, the NaNbO3/Bi2O2CO3 composite exhibits exceptional performance in the photocatalytic nitrogen fixation reaction. This study expands the application of S-scheme photocatalysts in the field of N2 reduction and provides insights into the preparation of efficient S-scheme photocatalysts.

Triptycene-Based Polymer-Incorporated CdxZn1-xS Nanorod with Enhanced Interfacial Charge Transfer for Stable Photocatalytic Hydrogen Production in Seawater.

Solar-driven hydrogen (H2) generation from seawater exhibits great economic value in addressing the urgent energy shortage yet faces challenges from the severe salt-deactivation effect, which could result in the consumption of photoinduced charges and decomposition of catalysts. Herein, a triptycene-based polymer was coated on the surface of a CdxZn1-xS nanorod to form a core-shell heterojunction (TCP@CZS) by using the in situ Suzuki reaction for photocatalytic H2 production from water/seawater splitting. The introduction of TCP can provide a large surface area, enrich the active site, and boost charge transfer for the proton reduction reaction. Benefiting from it, optimal TCP@CZS indicated a H2 evolution rate of 93.88 mmol h-1 g-1 with Na2S/Na2SO3 in natural seawater under simulated solar light irradiation, which was 2.2 and 1.1 times higher than that of pure Cd0.6Zn0.4S and that in pure water, respectively. Besides, the apparent quantum efficiency (AQE) of TCP@CZS-3 under 420 nm light irradiation was 22.6% in seawater. This work highlights the feasibility of the triptycene-based porous organic polymer as an efficient catalyst for solar energy conversion in seawater.

One-step preparation of MoOx/ZnS/ZnO composite and its excellent performance in piezocatalytic degradation of Rhodamine B under ultrasonic vibration.

This paper synthesized a new type of ternary piezoelectric catalyst MoOx/ZnS/ZnO (MZZ) by a one-step method. The catalytic degradation of Rhodamine B (RhB) solution (10 µg/g, pH = 7.0) shows that the composite catalyst has excellent piezoelectric catalytic activity under ultrasonic vibration (40 kHz). The piezoelectric degradation rate of the optimal sample reached 0.054 min-1, which was about 2.5 times that of pure ZnO. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Raman, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) technologies were used to analyze the structure, morphology, and interface charge transfer properties of the MZZ piezocatalysts. The results showed that the composite catalyst may have a core-shell structure. ZnS is coated on the surface of ZnO, while MoOx adheres to the surface of ZnS. This structure endowed MZZ larger specific surface area than ZnO, which benefits the RhB adsorption. More importantly, the formed heterojunction structure between ZnS and ZnO promotes the separation of positive and negative charges induced by the piezoelectric effect. MoOx species may act as a charge trap to further promote more carriers to participate in the reaction. In addition, MoOx may also be beneficial in adsorbing dyes. Active species capture experiments show that superoxide radicals and holes are the main active species in piezoelectric catalytic reactions on MZZ catalysts.

Circ_0009910 Serves as miR-361-3p Sponge to Promote the Proliferation, Metastasis, and Glycolysis of Gastric Cancer via Regulating SNRPA.

Circular RNA (circRNA) has been proved to be a key regulator of gastric cancer (GC) progression. Circ_0009910 was found to be highly expressed in GC and related to GC progression, but its role and mechanism in GC progression need to be further improved. Our study aims to further reveal circ_0009910 roles in GC progression and elucidate its potential molecular mechanism. The expression of circ_0009910, microRNA (miR)-361-3p, and small nuclear ribonucleoprotein polypeptide A (SNRPA) mRNA was measured by quantitative real-time PCR. Protein expression was determined using western blot analysis. Cell proliferation, migration, invasion, and apoptosis were evaluated using EDU staining, transwell assay, and flow cytometry. Cell glycolysis were assessed by detecting glucose consumption, lactate production, and glycolysis-related markers protein expression. The relationship between miR-361-3p and circ_0009910 or SNRPA was confirmed by RNA pull-down assay and dual-luciferase reporter assay. In vivo experiments were performed to explore the effect of circ_0009910 silencing on GC tumorigenesis. Circ_0009910 and SNRPA were upregulated in GC tumor tissues and cells. Knockdown of circ_0009910 or SNRPA could inhibit GC cell proliferation, migration, invasion, glycolysis, and promote apoptosis. Circ_0009910 could sponge miR-361-3p, and miR-361-3p could target SNRPA. Further experiments confirmed that circ_0009910 positively regulated SNRPA by sponging miR-361-3p. Additionally, SNRPA overexpression abolished the negative regulation of circ_0009910 silencing on GC progression. Furthermore, silenced circ_0009910 also reduced GC tumorigenesis in vivo. Our data showed that circ_0009910 might be a target for GC treatment, which could promote GC proliferation, metastasis, and glycolysis by the miR-361-3p/SNRPA axis.

The chloroplast genome of Amygdalus L. (Rosaceae) reveals the phylogenetic relationship and divergence time.

BACKGROUND: Limited access to genetic information has greatly hindered our understanding of the molecular evolution, phylogeny, and differentiation time of subg. Amygdalus. This study reported complete chloroplast (cp) genome sequences of subg. Amygdalus, which further enriched the available valuable resources of complete cp genomes of higher plants and deepened our understanding of the divergence time and phylogenetic relationships of subg. Amygdalus. RESULTS: The results showed that subg. Amygdalus species exhibited a tetrad structure with sizes ranging from 157,736 bp (P. kansuensis) to 158,971 bp (P. davidiana), a pair of inverted repeat regions (IRa/IRb) that ranged from 26,137-26,467 bp, a large single-copy region that ranged from 85,757-86,608 bp, and a small single-copy region that ranged from 19,020-19,133 bp. The average GC content of the complete cp genomes in the 12 species was 36.80%. We found that the structure of the subg. Amygdalus complete cp genomes was highly conserved, and the 12 subg. Amygdalus species had an rps19 pseudogene. There was not rearrangement of the complete cp genome in the 12 subg. Amygdalus species. All 12 subg. Amygdalus species clustered into one clade based on both Bayesian inference and maximum likelihood. The divergence time analyses based on the complete cp genome sequences showed that subg. Amygdalus species diverged approximately 15.65 Mya. CONCLUSION: Our results provide data on the genomic structure of subg. Amygdalus and elucidates their phylogenetic relationships and divergence time.

High-Throughput Screen Identifies Host and Microbiota Regulators of Intestinal Barrier Function.

BACKGROUND & AIMS: The intestinal barrier protects intestinal cells from microbes and antigens in the lumen-breaches can alter the composition of the intestinal microbiota, the enteric immune system, and metabolism. We performed a screen to identify molecules that disrupt and support the intestinal epithelial barrier and tested their effects in mice. METHODS: We performed an imaging-based, quantitative, high-throughput screen (using CaCo-2 and T84 cells incubated with lipopolysaccharide; tumor necrosis factor; histamine; receptor antagonists; and libraries of secreted proteins, microbial metabolites, and drugs) to identify molecules that altered epithelial tight junction (TJ) and focal adhesion morphology. We then tested the effects of TJ stabilizers on these changes. Molecules we found to disrupt or stabilize TJs were administered mice with dextran sodium sulfate-induced colitis or Citrobacter rodentium-induced intestinal inflammation. Colon tissues were collected and analyzed by histology, fluorescence microscopy, and RNA sequencing. RESULTS: The screen identified numerous compounds that disrupted or stabilized (after disruption) TJs and monolayers of epithelial cells. We associated distinct morphologic alterations with changes in barrier function, and identified a variety of cytokines, metabolites, and drugs (including inhibitors of actomyosin contractility) that prevent disruption of TJs and restore TJ integrity. One of these disruptors (putrescine) disrupted TJ integrity in ex vivo mouse colon tissues; administration to mice exacerbated colon inflammation, increased gut permeability, reduced colon transepithelial electrical resistance, increased pattern recognition receptor ligands in mesenteric lymph nodes, and decreased colon length and survival times. Putrescine also increased intestine levels and fecal shedding of viable C rodentium, increased bacterial attachment to the colonic epithelium, and increased levels of inflammatory cytokines in colon tissues. Colonic epithelial cells from mice given putrescine increased expression of genes that regulate metal binding, oxidative stress, and cytoskeletal organization and contractility. Co-administration of taurine with putrescine blocked disruption of TJs and the exacerbated inflammation. CONCLUSIONS: We identified molecules that disrupt and stabilize intestinal epithelial TJs and barrier function and affect development of colon inflammation in mice. These agents might be developed for treatment of barrier intestinal impairment-associated and inflammatory disorders in patients, or avoided to prevent inflammation.

PEGylated Nanoscale Metal-Organic Frameworks for Targeted Cancer Imaging and Drug Delivery.

Nanoscale metal-organic frameworks (nMOFs) are a unique type of hybrid materials, which are broadly applicable as cargo delivery systems. However, the relatively low material stability and insufficient cancer cell interacting capacity have limited nMOFs' applications in cancer theranostics. Herein, a zirconium-based nMOF UiO-66-N3 was synthesized, and its surface was covalently functionalized with alkyne-containing polyethylene glycol (PEG) via the azide-alkyne click chemistry. After that, F3 peptide was attached for targeting of cancer cells (the material was denoted as UiO-66-PEG-F3). Doxorubicin (DOX) served as a therapeutic drug and a fluorescent label in this study, and it was transported into UiO-66-PEG conjugates with sufficient drug loading efficiency. pH-responsive release of DOX from UiO-66 conjugates was witnessed. The structural integrity of UiO-66-N3 was maintained post the surface modification process. Flow cytometry and confocal fluorescence microscopy revealed that DOX/UiO-66-PEG-F3 had stronger accumulation in MDA-MB-231 cells (nucleolin+) compared with DOX/UiO-66-PEG. In order to track the pharmacokinetic behavior (organ distribution profile) in vivo, the positron-emitting zirconium-89 (89Zr) was incorporated into UiO-66-N3. Similar PEGylation and F3 peptide conjugation resulted in the formation of 89Zr-UiO-66-PEG-F3. Serial positron emission tomography (PET) imaging demonstrated that the preferential accumulation of 89Zr-UiO-66-PEG-F3 in MDA-MB-231 tumors, and their liver clearance was faster than PEGylated UiO-66 using noncovalent methods. Thus, the PEGylated nMOFs using covalent strategies may find broad application in future cancer theranostics.

Continuous Resonance Tuning without Blindness by Applying Nonlinear Properties of PIN Diodes.

Metamaterial antennas consisting of periodical units are suitable for achieving tunable properties by employing active elements to each unit. However, for compact metamaterials with a very limited number of periodical units, resonance blindness exists. In this paper, we introduce a method to achieve continuous tuning without resonance blindness by exploring hence, taking advantage of nonlinear properties of PIN diodes. First, we obtain the equivalent impedance of the PIN diode through measurements, then fit these nonlinear curves with mathematical expressions. Afterwards, we build the PIN diode model with these mathematical equations, making it compatible with implementing co-simulation between the passive electromagnetic model and the active element of PIN diodes and, particularly, the nonlinear effects can be considered. Next, we design a compact two-unit metamaterial antenna as an example to illustrate the electromagnetic co-simulation. Finally, we implement the experiments with a micro-control unit to validate this method. In addition, the nonlinear stability and the supplying voltage tolerance of nonlinear states for both two kinds of PIN diodes are investigated as well. This method of obtaining smooth tuning with nonlinear properties of PIN diodes can be applied to other active devices, if only PIN diodes are utilized.

In situ preparation of g-C3N4/Bi4O5I2 complex and its elevated photoactivity in Methyl Orange degradation under visible light.

A graphite carbon nitride (g-C3N4) modified Bi4O5I2 composite was successfully prepared in-situ via the thermal treatment of a g-C3N4/BiOI precursor at 400°C for 3 hr. The as-prepared g-C3N4/Bi4O5I2 showed high photocatalytic performance in Methyl Orange (MO) degradation under visible light. The best sample presented a degradation rate of 0.164 min-1, which is 3.2 and 82 times as high as that of Bi4O5I2 and g-C3N4, respectively. The g-C3N4/Bi4O5I2 was characterized by X-ray powder diffractometer (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman, X-ray photoelectron spectroscopy (XPS), ultraviolet-visible diffuse reflectance spectra (DRS), electrochemical impedance spectroscopy (EIS) and transient photocurrent response in order to explain the enhanced photoactivity. Results indicated that the decoration with a small amount of g-C3N4 influenced the specific surface area only slightly. Nevertheless, the capability for absorbing visible light was improved measurably, which was beneficial to the MO degradation. On top of that, a strong interaction between g-C3N4 and Bi4O5I2 was detected. This interplay promoted the formation of a favorable heterojunction structure and thereby enhanced the charge separation. Thus, the g-C3N4/Bi4O5I2 composite presented greater charge separation efficiency and much better photocatalytic performance than Bi4O5I2. Additionally, g-C3N4/Bi4O5I2 also presented high stability. •O2- and holes were verified to be the main reactive species.

Reflection Airy distribution of a Fabry-Pérot resonator and its application in waveguide loss measurement.

The reflection Airy distribution (RAD) of a Fabry-Pérot (F-P) resonator is deduced with the consideration of the mode coupling between the cavity resonance field and the initial back-reflected field at the input F-P resonator facet, as well as the influence of the loss/gain factor. A waveguide loss/gain measurement method is proposed based on the measurement of the finesse of the RAD, which is intrinsically free from the influence of the coupling loss and the substrate scattering noise. The waveguide loss can be measured with a simple single-facet coupling setup, considerably reducing the coupling difficulty and the complexity of the measurement system while achieving the same or better measurement accuracy as that of the transmission F-P method.

Highway investment in deindustrialization: A territorial analysis of office property transactions in Hong Kong, 2002-2013.

Transportation investment continues to grow in aid of economic competitiveness and environmental sustainability worldwide, but limited empirical research has been conducted on the changing value of highway investment in deindustrializing economies. This study examines the influences of highway proximity and traffic on office values in Hong Kong using hedonic price analysis on 13,670 transactions around highway interchanges for 2002-2013. Our hedonic regressions, controlling for unobservable district effects and incorporating instrumental variables, reveal that the associations of highway proximity and traffic with office prices appear to exhibit mixed results across Hong Kong's three territorial divisions. The negative externalities generated by excess traffic cancel out the accessibility benefits of highway proximity on Hong Kong Island, where densely built-up office districts are suited for amenity-sensitive knowledge businesses that tend to create value-added services through face-to-face communications. By contrast, highway proximity uplifts the value of office properties with lower transportation costs and higher market accessibility in Kowloon and the New Territories, where spacious workplaces near hub-port and logistics facilities are advantageous for mobility-driven trade and transportation businesses that tend to increase value-added throughput in cross-border relations with mainland China. These territorial findings are of particular importance for progressive policymakers to deploy the strategic applications of underground bypasses, greenway creation, interchange improvement, congestion charges, and smart technology to manage mobility and alleviate disamenity, accompanied by supportive public transit services and adaptive land-use rezoning, in the dynamic and complex process of deindustrialization.

Application of Ag/AgBr/GdVO4 composite photocatalyst in wastewater treatment.

Ag/AgBr/GdVO4 composite photocatalysts were designed and synthesized in this paper. The physical and chemical structures, as well as optical properties of the synthesized composite were investigated via XRD, XPS, TEM, and UV-vis. It is found that the composite showed a ternary heterojunction structure of Ag, AgBr and GdVO4. Meanwhile, it has a high intensity of light current, indicating its high separation efficiency of electron and hole. Photocatalytic oxidation of rhodamine B (RhB) under visible light irradiation was performed to investigate the activity of the Ag/AgBr/GdVO4 composite. Result indicates that it shows excellent photocatalytic activity. Under visible light irradiation for 12min, about 80% of RhB (30µmol/L) was degraded. The degradation rate is estimated to be 0.253 min-1, which is three times higher than that of pure AgBr. The high photoactivity can be ascribed to the synergetic effect of AgBr, GdVO4, and Ag nanoparticle in separation of electron-hole pairs.

LncRNA-SNHG1 contributes to gastric cancer cell proliferation by regulating DNMT1.

OBJECTIVE: The objective of this study was to determine the expression of long-chain non-coding RNA SNHG1 (lncRNA-SNHG1) in gastric carcinoma and explore its function on cancer cell proliferation. METHODS: The expression of lncRNA-SNHG1 in tumor tissues and corresponding adjacent tissues from 50 patients with gastric cancer was detected with realtime-PCR. The relationships between the expression of lncRNA-SNHG1 and clinicopathological features of gastric cancer patients were analyzed. Survival analysis was performed to study the correlation between lncRNA SNHG1 expression and patient prognosis. To assess the effect of LncRNA SNHG1 on proliferation in cancer cells, cell viability and colony formation assays were conducted when lncRNA SNHG1 was upregulated or downregulated by Lentivirus or plasmid in gastric cancer cells. Furthermore, in vivo tumor assay was performed to confirm the impact of lncRNA SNHG1 on proliferation of gastric cancer. RESULTS: The expression of lncRNA SNHG1 in gastric cancer tissues was significantly higher than that in adjacent tissues and was correlated with TNM stage, T stage, and lymph node metastasis. The survival time of patients with higher expression level of lncRNA-SNHG1 was significantly lower than that of the lower expression level. LncRNA-SNHG1 accelerated the proliferation of gastric cancer cells obviously and increased the expression of DNMT1. CONCLUSION: LncRNA SNHG1 promotes DNMT1 expression, which facilitates the gastric cancer proliferation.

PART-1 functions as a competitive endogenous RNA for promoting tumor progression by sponging miR-143 in colorectal cancer.

LncRNAs were altered in several cancers and played a crucial role in various biological activities and progressions of different diseases, including proliferation, chemical resistance, and metastasis. In the present study, we revealed that prostrate androgen-regulated transcript-1 (PART-1) was highly expressed in colorectal cancer cells and tissues, and knockdown of PART-1 suppressed cell proliferation and metastasis, both in vitro and in vivo. In addition, PART-1 functioned as a ceRNA of DNMT3A, by sponging miR-143. Finally,PART-1 induced tumor progression by regulating DNMT3A.