RESUMEN
Neurotrophic natural products hold potential as privileged structures for the development of therapeutic agents against neurodegeneration. However, only a few studies have been conducted to investigate a common pharmacophoric motif and structure-activity relationships (SARs). Here, an investigation of structurally more simple analogues of neurotrophic sesquiterpenes of the illicium family is presented. A concise synthetic route enables preparation of the carbon framework of (±)-Merrilactoneâ A and (±)-Anislactoneâ A/B on a gram scale. This has allowed access to a series of structural analogues by modification of the core structure, including variation of oxidation levels and alteration of functional groups. In total, 15 derivatives of the natural products have been synthesized and tested for their neurite outgrowth activities. Our studies indicate that the promising biological activity can be retained by structurally simpler natural product analogues, which are accessible by a straightforward synthetic route.
Asunto(s)
Illicium/química , Neurogénesis/efectos de los fármacos , Neuronas/efectos de los fármacos , Sesquiterpenos/química , Sesquiterpenos/farmacología , Animales , Productos Biológicos/síntesis química , Productos Biológicos/química , Productos Biológicos/farmacología , Línea Celular , Proliferación Celular/efectos de los fármacos , Ratones , Modelos Moleculares , Neuritas/efectos de los fármacos , Neuritas/metabolismo , Neuronas/citología , Sesquiterpenos/síntesis química , EstereoisomerismoRESUMEN
The synthesis of all 20 common natural proteinogenic and 4 otherα-amino acid-isosteric α-amino tetrazoles has been accomplished, whereby the carboxyl group is replaced by the isosteric 5-tetrazolyl group. The short process involves the use of the key Ugi tetrazole reaction followed by deprotection chemistries. The tetrazole group is bioisosteric to the carboxylic acid and is widely used in medicinal chemistry and drug design. Surprisingly, several of the common α-amino acid-isosteric α-amino tetrazoles are unknown up to now. Therefore a rapid synthetic access to this compound class and non-natural derivatives is of high interest to advance the field.
Asunto(s)
Aminoácidos/síntesis química , Ácidos Carboxílicos/química , Tetrazoles/síntesis química , Aminoácidos/química , Diseño de Fármacos , Estructura Molecular , Tetrazoles/químicaRESUMEN
A simple Ugi tetrazole multicomponent reaction allows the synthesis of a novel macrocyclic cyclen derivative with four appendant tetrazole arms in just two steps in excellent yields. This ligand, called TEMDO, turns out to have a high complexation affinity with lanthanoid metals. Here we describe the design, synthesis, solid-state structure, binding constant, and some MRI applications of the Gd-TEMDO complex as the first example of a congeneric family of oligo-amino tetrazoles.
Asunto(s)
Gadolinio/química , Compuestos Heterocíclicos/síntesis química , Compuestos Organometálicos/síntesis química , Tetrazoles/síntesis química , Medios de Contraste/química , Ciclamas , Compuestos Heterocíclicos/química , Ligandos , Imagen por Resonancia Magnética , Estructura Molecular , Compuestos Organometálicos/química , Tetrazoles/químicaRESUMEN
The binding properties of neutral halogen-bond donors (XB donors) bearing two multidentate Lewis acidic motifs toward halides were investigated. Employing polyfluorinated and polyiodinated terphenyl and quaterphenyl derivatives as anion receptors, we obtained X-ray crystallographic data of the adducts of three structurally related XB donors with tetraalkylammonium chloride, bromide, and iodide. The stability of these XB complexes in solution was determined by isothermal titration calorimetry (ITC), and the results were compared to X-ray analyses as well as to calculated binding patterns in the gas phase. Density functional theory (DFT) calculations on the gas-phase complexes indicated that the experimentally observed distortion of the XB donors during multiple multidentate binding can be reproduced in 1:1 complexes with halides, whereas adducts with two halides show a symmetric binding pattern in the gas phase that is markedly different from the solid state structures. Overall, this study demonstrates the limitations in the transferability of binding data between solid state, solution, and gas phase in the study of complex multidentate XB donors.
RESUMEN
The dinuclear zinc complex reported by us is to date the most active zinc catalyst for the co-polymerization of cyclohexene oxide (CHO) and carbon dioxide. However, co-polymerization experiments with propylene oxide (PO) and CO2 revealed surprisingly low conversions. Within this work, we focused on clarification of this behavior through experimental results and quantum chemical studies. The combination of both results indicated the formation of an energetically highly stable intermediate in the presence of propylene oxide and carbon dioxide. A similar species in the case of cyclohexene oxide/CO2 co-polymerization was not stable enough to deactivate the catalyst due to steric repulsion.
RESUMEN
Poly(3-hydroxybutyrate) (PHB) is produced by numerous bacteria as carbon and energy reserve storage material. Whereas nature only produces PHB in its strictly isotactic (R) form, homogeneous catalysis, when starting from racemic (rac) ß-butyrolactone (BL) as monomer, can in fact produce a wide variety of tacticities. The variation of the metal center and the surrounding ligand structure enable activity as well as tacticity tuning. However, no homogeneous catalyst exists to date that is easy to modify, highly active, and able to produce PHB with high isotacticities from rac-ß-BL. Therefore, in this work, the reaction kinetics of various 2-methoxyethylamino-bis(phenolate) lanthanide (Ln=Sm, Tb, Y, Lu) catalysts are examined in detail. The order in monomer and catalyst are determined to elucidate the reaction mechanism and the results are correlated with DFT calculations of the catalytic cycle. Furthermore, the enthalpies and entropies of the rate-determining steps are determined through temperature-dependent in situ IR measurements. Experimental and computational results converge in one specific mechanism for the ring-opening polymerization of BL and even allow us to rationalize the preference for syndiotactic PHB.
RESUMEN
Functionalization of the PNP pincer ligand backbone allows for a comparison of the dialkyl amido, vinyl alkyl amido, and divinyl amido ruthenium(II) pincer complex series [RuCl{N(CH2 CH2 PtBu2 )2 }], [RuCl{N(CHCHPtBu2 )(CH2 CH2 PtBu2 )}], and [RuCl{N(CHCHPtBu2 )2 }], in which the ruthenium(II) ions are in the extremely rare square-planar coordination geometry. Whereas the dialkylamido complex adopts an electronic singlet (S=0) ground state and energetically low-lying triplet (S=1) state, the vinyl alkyl amido and the divinyl amido complexes exhibit unusual triplet (S=1) ground states as confirmed by experimental and computational examination. However, essentially non-magnetic ground states arise for the two intermediate-spin complexes owing to unusually large zero-field splitting (D>+200â cm(-1) ). The change in ground state electronic configuration is attributed to tailored pincer ligand-to-metal π-donation within the PNP ligand series.
Asunto(s)
Complejos de Coordinación/química , Rutenio/química , Alquilación , Amidas/química , Butadienos/química , Espectroscopía de Resonancia Magnética , Modelos Moleculares , Compuestos de Vinilo/químicaRESUMEN
The proteinprotein interaction of p53 and MDM2/X is a promising non genotoxic anticancer target. A rapid and efficient methodology was developed to synthesize the 2,30-bis(10H-indole) heterocyclic scaffold 2 as ester, acid and amide derivatives. Their binding affinity with MDM2 was evaluated using both fluorescence polarization (FP) assay and HSQC experiments, indicating good inhibition and a perfect starting point for further optimizations.
Asunto(s)
Compuestos Heterocíclicos/química , Proteínas Proto-Oncogénicas c-mdm2/antagonistas & inhibidores , Proteína p53 Supresora de Tumor/antagonistas & inhibidores , Sitios de Unión , Polarización de Fluorescencia , Compuestos Heterocíclicos/síntesis química , Compuestos Heterocíclicos/metabolismo , Indoles/química , Simulación del Acoplamiento Molecular , Dominios y Motivos de Interacción de Proteínas , Estructura Terciaria de Proteína , Proteínas Proto-Oncogénicas c-mdm2/metabolismo , Proteína p53 Supresora de Tumor/metabolismoRESUMEN
In this article, we report the synthesis of a tridentate carbene-centered bisphosphine ligand precursor and its complexes. The developed four-step synthetic strategy of a new PC(BIm)P pincer ligand represents the derivatization of benzimidazole in the first and third positions by (diphenylphosphoryl)methylene synthone, followed by phosphine deprotection and subsequent insertion of a noncoordinating anion. The obtained ligand precursor undergoes complexation, with PdCl2 and [µ-OCH3Rh(COD)]2 smoothly forming the target organometallics [PC(BIm)PPdCl][PF6] and [PC(BIm)PRh(L)][PF6] under mild hydrogenation conditions. A more detailed study of the rhodium complexes [PC(BIm)PRh(L)][PF6] reveals significant thermal stability of the PC(BIm)PRh moiety in the solid state as well as in solution. The chemical behavior of 1,3-bis(diphenylphosphinomethylene)benzimidazol-2-ylrhodium acetonitrile hexafluorophosphate has been screened under decarbonylation, hydrogenation, and hydroboration reaction conditions. Thus, the PC(BIm)PRh(I) complex is a sufficiently stable compound, with the potential to be applied in catalysis.
RESUMEN
Three novel cis-to-trans-converted dimolybdenum(II) complexes, trans-[Mo2(O2C-Fc)2(DPPX)2][BF4]2 {2a-2c; DPPX = DPPA [N,N-bis(diphenylphosphino)amine], DPPM [1,1-bis(diphenylphosphino)methane], and DPPE [1,2-bis(diphenylphosphino)ethane], respectively}, were synthesized through the insertion of bulky diphosphine ligands, which force a permanent trans arrangement, as evidenced by X-ray crystallography and density functional theory calculations. All compounds were characterized by means of NMR, UV-vis, and IR spectroscopy as well as thermogravimetry-mass spectrometry measurements. Interestingly, uncommon UV-vis transitions and oxidation sequences were observed compared to previously reported ones. As verified by electrochemical measurements, all synthesized complexes show two separate one-electron-redox processes assigned to subsequent oxidations of the two redox-active ferrocenecarboxylate ligands, with a split of ca. 70 mV. This behavior reveals electronic interaction between the two equatorially trans-positioned ferrocenyl units. The presented work provides new insights into the rational synthesis of electronically coupled trans-coordinated Mo2 systems, paving the way toward the design of linear multicenter redox-active oligomers.
RESUMEN
Scaffold diversity is key in the ongoing exercise of discovery of novel bioactive compounds using high throughput screening (HTS). Based on the Ugi tetrazole synthesis we have designed novel bi- and tri-cyclic scaffolds featuring interesting pharmacophore properties. The compounds of the scaffold (B) are synthesizable in large diversity and numbers in two steps using (hetero)phenylethylamines, HN3, oxo components and iscyanoacetaldehyde(dimethylacetale). The chemistry is amenable to parallel synthesis and is used to enhance and fill the screening decks of the European Lead factory (ELF). Here, we are reporting full experimental details, scope and limitations of the reaction, cheminformatic analysis and the 3D structures of selected compounds.
Asunto(s)
Descubrimiento de Drogas , Compuestos Policíclicos/síntesis química , Tetrazoles/síntesis química , Modelos Moleculares , Estructura MolecularRESUMEN
A well-defined three-point interaction based solely on halogen bonding is presented. X-ray structural analyses of tridentate halogen bond donors (halogen-based Lewis acids) with a carefully chosen triamine illustrate the ideal geometric fit of the Lewis acidic axes of the former with the Lewis basic centers of the latter. Titration experiments reveal that the corresponding binding constant is about 3 orders of magnitude higher than that with a comparable monodentate amine. Other, less perfectly fitting multidentate amines also bind markedly weaker. Multipoint interactions like the one presented herein are the basis of molecular recognition, and we expect this principle to further establish halogen bonding as a reliable tool for solution-phase applications.
RESUMEN
The X-ray crystal and NMR spectroscopic structures of the peptide drug candidate Cilengitide (cyclo(RGDf(NMe)Val)) in various solvents are obtained and compared in addition to the integrin receptor bound conformation. The NMR-based solution structures exhibit conformations closely resembling the X-ray structure of Cilengitide bound to the head group of integrin αvß3. In contrast, the structure of pure Cilengitide recrystallized from methanol reveals a different conformation controlled by the lattice forces of the crystal packing. Molecular modeling studies of the various ligand structures docked to the αvß3 integrin revealed that utilization of the solid-state conformation of Cilengitide leads-unlike the solution-based structures-to a mismatch of the ligand-receptor interactions compared with the experimentally determined structure of the protein-ligand complex. Such discrepancies between solution and crystal conformations of ligands can be misleading during the structure-based lead optimization process and should thus be taken carefully into account in ligand orientated drug design.
Asunto(s)
Integrina alfaVbeta3/química , Venenos de Serpiente/química , Cristalografía por Rayos X , Diseño de Fármacos , Modelos Moleculares , Conformación Molecular , Resonancia Magnética Nuclear Biomolecular , Conformación Proteica , SolucionesRESUMEN
Reaction of [RuCl(CNN)(dppb)] (1-Cl) (HCNN=2-aminomethyl-6-(4-methylphenyl)pyridine; dppb=Ph2 P(CH2 )4 PPh2 ) with NaOCH2 CF3 leads to the amine-alkoxide [Ru(CNN)(OCH2 CF3 )(dppb)] (1-OCH2 CF3 ), whose neutron diffraction study reveals a short RuOâ â â HN bond length. Treatment of 1-Cl with NaOEt and EtOH affords the alkoxide [Ru(CNN)(OEt)(dppb)]â (EtOH)n (1-OEtâ n EtOH), which equilibrates with the hydride [RuH(CNN)(dppb)] (1-H) and acetaldehyde. Compound 1-OEtâ n EtOH reacts reversibly with H2 leading to 1-H and EtOH through dihydrogen splitting. NMR spectroscopic studies on 1-OEtâ n EtOH and 1-H reveal hydrogen bond interactions and exchange processes. The chloride 1-Cl catalyzes the hydrogenation (5 atm of H2 ) of ketones to alcohols (turnover frequency (TOF) up to 6.5×10(4) h(-1) , 40 °C). DFT calculations were performed on the reaction of [RuH(CNN')(dmpb)] (2-H) (HCNN'=2-aminomethyl-6-(phenyl)pyridine; dmpb=Me2 P(CH2 )4 PMe2 ) with acetone and with one molecule of 2-propanol, in alcohol, with the alkoxide complex being the most stable species. In the first step, the Ru-hydride transfers one hydrogen atom to the carbon of the ketone, whereas the second hydrogen transfer from NH2 is mediated by the alcohol and leads to the key "amide" intermediate. Regeneration of the hydride complex may occur by reaction with 2-propanol or with H2 ; both pathways have low barriers and are alcohol assisted.
Asunto(s)
Cetonas/química , Piridinas/química , Rutenio/química , Catálisis , Hidrogenación , Modelos MolecularesRESUMEN
Initiation of rare earth metal-mediated vinylphosphonate polymerization with unbridged rare earth metallocenes (Cp2LnX) follows a complex reaction pathway. Depending on the nature of X, initiation can proceed either via abstraction of the acidic α-CH of the vinylphosphonate (e.g., for X = Me, CH2TMS), via nucleophilic transfer of X to a coordinated monomer (e.g., for X = Cp, SR) or via a monomer (i.e., donor)-induced ligand-exchange reaction forming Cp3Ln in equilibrium (e.g., for X = Cl, OR), which serves as the active initiating species. As determined by mass spectrometric end group analysis, different initiations may also occur simultaneously (e.g., for X = N(SiMe2H)2). A general differential approach for the kinetic analysis of living polymerizations with fast propagation and comparatively slow initiation is presented. Time-resolved analysis of monomer conversion and molecular weights of the formed polymers allow the determination of the initiator efficiency throughout the whole reaction. Using this normalization method, rare earth metal-mediated vinylphosphonate GTP is shown to follow a Yasuda-type monometallic propagation mechanism, with an SN2-type associative displacement of the polymer phosphonate ester by a monomer as the rate-determining step. The propagation rate of vinylphosphonate GTP is mainly determined by the activation entropy, i.e. the change of rotational and vibrational restrictions within the eight-membered metallacycle in the rate-determining step as a function of the steric demand of the metallacycle side chains and the steric crowding at the metal center.
Asunto(s)
Metales de Tierras Raras/química , Organofosfonatos/síntesis química , Compuestos de Vinilo/síntesis química , Estructura Molecular , Organofosfonatos/química , Polimerizacion , Compuestos de Vinilo/químicaRESUMEN
Tricks of the trade: Because intramolecular Cu-catalyzed access to bicyclo[4.2.0]octanes is not feasible, an oxygen bridge was introduced to facilitate the [2+2] photocycloaddition. Starting from compounds similar to 1, products such as 2 could be obtained enantioselectiviely in three steps after ring-opening metathesis (see scheme).
RESUMEN
Subsequent mild cyclization of aromatic substrates by Pictet-Spengler condensation stereoselectively gave new tricyclic compounds. Examples are described in decent yields over two steps in one pot, and a crystal structure is also presented to support the proposed structures.
Asunto(s)
Compuestos Heterocíclicos con 3 Anillos/síntesis química , Ciclización , Isoquinolinas/síntesis química , Modelos Moleculares , Estructura Molecular , EstereoisomerismoRESUMEN
Yttrocene-carboxylate complex [Cp*2Y(OOCAr(Me))] (Cp*=C5Me5, Ar(Me) =C6H2Me3-2,4,6) was synthesized as a spectroscopically versatile model system for investigating the reactivity of alkylaluminum hydrides towards rare-earth-metal carboxylates. Equimolar reactions with bis-neosilylaluminum hydride and dimethylaluminum hydride gave adduct complexes of the general formula [Cp*2Y(µ-OOCAr(Me))(µ-H)AlR2] (R=CH2SiMe3, Me). The use of an excess of the respective aluminum hydride led to the formation of product mixtures, from which the yttrium-aluminum-hydride complex [{Cp*2Y(µ-H)AlMe2(µ-H)AlMe2(µ-CH3)}2] could be isolated, which features a 12-membered-ring structure. The adduct complexes [Cp*2Y(µ-OOCAr(Me))(µ-H)AlR2] display identical (1)J(Y,H) coupling constants of 24.5 Hz for the bridging hydrido ligands and similar (89)Y NMR shifts of δ=-88.1 ppm (R=CH2SiMe3) and δ=-86.3 ppm (R=Me) in the (89)Y DEPT45 NMR experiments.
RESUMEN
(Acetoxymethyl)silanes 2, 7a-c, and 10a-c with at least one alkoxy group, of the general formula (AcOCH2 )Si(OR)3-n(CH3)n (R: Me, Et, iPr; n=0, 1, 2), were synthesized from the corresponding (chloromethyl)silanes 1, 6a-c, and 9a-c by treatment with potassium acetate under phase-transfer-catalysis conditions. These compounds were found to provide 2,2,5,5-organo-substituted 1,4-dioxa-2,5-disilacyclohexanes 3, 8a-c, and 11a-c if treated with organotin(IV) catalysts such as dioctyltin oxide. The reaction proceeds through transesterification of the acetoxy and alkoxy units followed by ring-closure to form a dimeric six-membered ring. The corresponding alkyl acetates are formed as the reaction by-products. With these mild conditions, the method overcomes the drawbacks of previously reported synthetic routes to furnish 2,2,5,5-tetramethyl-1,4-dioxa-2,5-disilacyclohexane (3) and even allows the synthesis of 1,4-dioxa-2,5-disilacyclohexanes bearing hydrolytically labile alkoxy substituents at the silicon atom in good yields and high purity. These new materials were fully characterized by NMR spectroscopy, elemental analysis, mass spectrometry, and X-ray analysis (trans-8a).
RESUMEN
The cyclen-based tetraphosphinate chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis[methylene(2-carboxyethyl)phosphinic acid] (DOTPI) comprises four additional carboxylic acid moieties for bioconjugation. The thermodynamic stability constants (logK(ML)) of metal complexes, as determined by potentiometry, were 23.11 for Cu(II), 20.0 for Lu(III), 19.6 for Y(III), and 21.0 for Gd(III). DOTPI was functionalized with four cyclo(Arg-Gly-Asp-D-Phe-Lys) (RGD) peptides through polyethylene glycol (PEG4) linkers. The resulting tetrameric conjugate DOTPI(RGD)4 was radiolabeled with (177)Lu and (64)Cu and showed improved labeling efficiency compared with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). The labeled compounds were fully stable in transchelation challenges against trisodium diethylenetriaminepentaacetate (DTPA) and disodium ethylenediaminetetraacetic acid (ETDA), in phosphate buffered saline (PBS), and human plasma. Integrin αvß3 affinities of the non-radioactive Lu(III) and Cu(II) complexes of DOTPI(RGD)4 were 18 times higher (both IC50 about 70 picomolar) than that of the c(RGDfK) peptide (IC50 = 1.3 nanomolar). Facile access to tetrameric conjugates and the possibility of radiolabeling with therapeutic and diagnostic radionuclides render DOTPI suitable for application in peptide receptor radionuclide imaging (PRRI) and therapy (PRRT).