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1.
Nature ; 627(8005): 754-758, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38093004

RESUMEN

Shock-breakout emission is light that arises when a shockwave, generated by the core-collapse explosion of a massive star, passes through its outer envelope. Hitherto, the earliest detection of such a signal was at several hours after the explosion1, although a few others had been reported2-7. The temporal evolution of early light curves should provide insights into the shock propagation, including explosion asymmetry and environment in the vicinity, but this has been hampered by the lack of multiwavelength observations. Here we report the instant multiband observations of a type II supernova (SN 2023ixf) in the galaxy M101 (at a distance of 6.85 ± 0.15 Mpc; ref. 8), beginning at about 1.4 h after the explosion. The exploding star was a red supergiant with a radius of about 440 solar radii. The light curves evolved rapidly, on timescales of 1-2 h, and appeared unusually fainter and redder than predicted by the models9-11 within the first few hours, which we attribute to an optically thick dust shell before it was disrupted by the shockwave. We infer that the breakout and perhaps the distribution of the surrounding dust were not spherically symmetric.

2.
J Exp Bot ; 73(22): 7285-7297, 2022 12 08.
Artículo en Inglés | MEDLINE | ID: mdl-36112496

RESUMEN

The majority of plant protein in the world's food supply is derived from soybean (Glycine max). Soybean is a key protein source for global animal feed and is incorporated into plant-based foods for people, including meat alternatives. Soybean protein content is genetically variable and is usually inversely related to seed oil content. ABI3-interacting protein 2 (AIP2) is an E3-RING ubiquitin ligase that targets the seed-specific transcription factor ABI3. Silencing both soybean AIP2 genes (AIP2a and AIP2b) by RNAi enhanced seed protein content by up to seven percentage points, with no significant decrease in seed oil content. The protein content enhancement did not alter the composition of the seed storage proteins. Inactivation of either AIP2a or AIP2b by a CRISPR-Cas9-mediated mutation increased seed protein content, and this effect was greater when both genes were inactivated. Transactivation assays in transfected soybean hypocotyl protoplasts indicated that ABI3 changes the expression of glycinin, conglycinin, 2S albumin, and oleosin genes, indicating that AIP2 depletion increased seed protein content by regulating activity of the ABI3 transcription factor protein. These results provide an example of a gene-editing prototype directed to improve global food security and protein availability in soybean that may also be applicable to other protein-source crops.


Asunto(s)
Sistemas CRISPR-Cas , Proteínas de Soja , Proteínas de Soja/genética , Semillas/genética , Factores de Transcripción , Aceites de Plantas , Ubiquitina , Ligasas
3.
BMC Plant Biol ; 18(1): 354, 2018 Dec 13.
Artículo en Inglés | MEDLINE | ID: mdl-30545296

RESUMEN

BACKGROUND: Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean's genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. RESULTS: In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. CONCLUSION: The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.


Asunto(s)
Glycine max/metabolismo , Proteínas de Plantas/metabolismo , Cotiledón/metabolismo , Cotiledón/fisiología , Cotiledón/ultraestructura , Electroforesis en Gel de Poliacrilamida , Endospermo/metabolismo , Endospermo/fisiología , Endospermo/ultraestructura , Regulación de la Expresión Génica de las Plantas , Focalización Isoeléctrica , Metaboloma , Microscopía Electrónica de Transmisión , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/fisiología , Glycine max/fisiología , Glycine max/ultraestructura , Estrés Fisiológico
4.
Plant Biotechnol J ; 13(4): 590-600, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-25400247

RESUMEN

Transgenic soya bean (Glycine max) plants overexpressing a seed-specific bacterial phytoene synthase gene from Pantoea ananatis modified to target to plastids accumulated 845 µg ß carotene g(-1) dry seed weight with a desirable 12:1 ratio of ß to α. The ß carotene accumulating seeds exhibited a shift in oil composition increasing oleic acid with a concomitant decrease in linoleic acid and an increase in seed protein content by at least 4% (w/w). Elevated ß-carotene accumulating soya bean cotyledons contain 40% the amount of abscisic acid compared to nontransgenic cotyledons. Proteomic and nontargeted metabolomic analysis of the mid-maturation ß-carotene cotyledons compared to the nontransgenic did not reveal any significant differences that would account for the altered phenotypes of both elevated oleate and protein content. Transcriptomic analysis, confirmed by RT-PCR, revealed a number of significant differences in ABA-responsive transcripton factor gene expression in the crtB transgenics compared to nontransgenic cotyledons of the same maturation stage. The altered seed composition traits seem to be attributed to altered ABA hormone levels varying transcription factor expression. The elevated ß-carotene, oleic acid and protein traits in the ß-carotene soya beans confer a substantial additive nutritional quality to soya beans.


Asunto(s)
Glycine max/metabolismo , Ácido Oléico/metabolismo , Proteínas de Plantas/metabolismo , Semillas/metabolismo , beta Caroteno/metabolismo , Ácido Abscísico/metabolismo , Carotenoides/biosíntesis , Ácido Graso Desaturasas/genética , Perfilación de la Expresión Génica , Plantas Modificadas Genéticamente , Glycine max/embriología , Glycine max/genética
6.
Plant Physiol ; 156(1): 330-45, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21398260

RESUMEN

The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP-) seeds are overtly identical to the wild type, maturing to similar size and weight, and in developmental ontogeny. The SP- seeds rebalance the proteome, maintaining wild-type levels of protein and storage triglycerides. The SP- soybeans were evaluated with systems biology techniques of proteomics, metabolomics, and transcriptomics using both microarray and next-generation sequencing transcript sequencing (RNA-Seq). Proteomic analysis shows that rebalancing of protein content largely results from the selective increase in the accumulation of only a few proteins. The rebalancing of protein composition occurs with small alterations to the seed's transcriptome and metabolome. The selectivity of the rebalancing was further tested by introgressing into the SP- line a green fluorescent protein (GFP) glycinin allele mimic and quantifying the resulting accumulation of GFP. The GFP accumulation was similar to the parental GFP-expressing line, showing that the GFP glycinin gene mimic does not participate in proteome rebalancing. The results show that soybeans make large adjustments to the proteome during seed filling and compensate for the shortage of major proteins with the increased selective accumulation of other proteins that maintains a normal protein content.


Asunto(s)
Glycine max/metabolismo , Metaboloma , Proteoma , Proteínas de Almacenamiento de Semillas/metabolismo , Transcriptoma , Aminoácidos/metabolismo , Cromatografía de Gases , Electroforesis en Gel Bidimensional , Perfilación de la Expresión Génica , Técnicas de Silenciamiento del Gen , Secuenciación de Nucleótidos de Alto Rendimiento , Espectrometría de Masas , Análisis de Secuencia por Matrices de Oligonucleótidos , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Proteínas de Almacenamiento de Semillas/genética , Semillas/genética , Semillas/metabolismo , Glycine max/genética , Glycine max/ultraestructura
8.
Plant Biotechnol J ; 6(8): 832-42, 2008 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-18694455

RESUMEN

Seeds possess a high intrinsic capacity for protein production that makes them a desirable bioreactor platform for the manufacture of transgenic products. One strategy to enhance foreign protein production involves exchanging the capacity to produce intrinsic proteins for the capacity to produce a high level of foreign proteins. Suppression of the alpha/alpha' subunit of beta-conglycinin storage protein synthesis in soybean has been shown previously to result in an increase in the accumulation of the glycinin storage protein, some of which is sequestered as proglycinin into de novo endoplasmic reticulum (ER)-derived protein bodies. The exchange of glycinin for conglycinin is quantitative, with the remodelled soybeans possessing a normal protein content with an altered proteome. The green fluorescent protein (GFP)-kdel reporter was transferred in a construct using the glycinin promoter and terminator to mimic glycinin gene expression. When expressed in soybean seeds, GFP-kdel accreted to form ER-derived protein bodies. The introgression of GFP-kdel into the alpha/alpha' subunit of the beta-conglycinin suppression background resulted in a fourfold enhancement of GFP-kdel accumulation to > 7% (w/w) of the total protein in soybean seeds. The resulting seeds accumulated a single population of ER membrane-bound protein bodies that contained both GFP-kdel and glycinin. Thus, the collateral proteome rebalancing that occurs with the suppression of intrinsic proteins in soybean can be exploited to produce an enhanced level of foreign proteins.


Asunto(s)
Globulinas/genética , Glycine max/genética , Semillas/genética , Proteínas de Soja/genética , Antígenos de Plantas , Electroforesis en Gel Bidimensional , Retículo Endoplásmico/metabolismo , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Genes Reporteros , Proteínas Fluorescentes Verdes/genética , Focalización Isoeléctrica , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/metabolismo , Regiones Promotoras Genéticas , Proteoma/genética , Proteoma/metabolismo , Proteínas de Almacenamiento de Semillas , Semillas/metabolismo , Semillas/ultraestructura , Glycine max/metabolismo
9.
J Pediatr Surg ; 53(6): 1203-1207, 2018 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-29636182

RESUMEN

BACKGROUND: Epidermal Growth Factor (EGF) reduces necrotizing enterocolitis (NEC). However, its high cost virtually prohibits clinical use. To reduce cost, soybean expressing human EGF was developed. Here we report effectiveness of soybean-derived EGF in experimental NEC. METHODS: Newborn rats were subjected to the NEC-inducing regimen of formula feeding and hypoxia. Formula was supplemented with extract from EGF-expressing or empty soybeans. NEC pathology was determined microscopically. Localization of tight junction proteins JAM-A and ZO-1 was examined by immunofluorescence and levels of mucosal COX-2 and iNOS mRNAs by real time PCR. RESULTS: Soybean extract amounts corresponding to 150µg/kg/day EGF caused considerable mortality, whereas those corresponding to 75µg/kg/day EGF were well tolerated. There was no significant difference in NEC scores between animals fed plain formula and formula supplemented with empty soybean extract. Soybean-EGF-supplemented formula at 75µg/kg/day EGF significantly decreased NEC, attenuated dissociation of JAM-A and ZO-1 proteins from tight junctions, and reduced intestinal expression of COX-2 and iNOS mRNAs. CONCLUSION: Supplementation with soybean-expressed EGF significantly decreased NEC in the rat model. Soybean-expressed EGF may provide an economical solution for EGF administration and prophylaxis of clinical NEC.


Asunto(s)
Enterocolitis Necrotizante/prevención & control , Factor de Crecimiento Epidérmico/uso terapéutico , Glycine max , Extractos Vegetales/uso terapéutico , Sustancias Protectoras/uso terapéutico , Animales , Animales Recién Nacidos , Ciclooxigenasa 2/metabolismo , Modelos Animales de Enfermedad , Enterocolitis Necrotizante/patología , Humanos , Fórmulas Infantiles , Recién Nacido , Recien Nacido Prematuro , Enfermedades del Prematuro/patología , Enfermedades del Prematuro/prevención & control , Mucosa Intestinal/metabolismo , Intestinos/patología , Moléculas de Adhesión de Unión/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , ARN Mensajero/metabolismo , Ratas Sprague-Dawley , Proteínas Recombinantes/uso terapéutico , Proteínas de la Zonula Occludens/metabolismo
10.
Front Plant Sci ; 7: 440, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27092158

RESUMEN

Aquaculture is the most rapidly growing segment of global animal production that now surpasses wild-capture fisheries production and is continuing to grow 10% annually. Sustainable aquaculture needs to diminish, and progressively eliminate, its dependence on fishmeal-sourced feed from over-harvested fisheries. Sustainable aquafeed sources will need to be primarily of plant-origin. Soybean is currently the primary global vegetable-origin protein source for aquaculture. Direct exchange of soybean meal for fishmeal in aquafeed has resulted in reduced growth rates due in part to soybean's anti-nutritional proteins. To produce soybeans for use in aquaculture feeds a new conventional line has been bred termed Triple Null by stacking null alleles for the feed-relevant proteins Kunitz Trypsin Inhibitor, lectin, and P34 allergen. Triple Null is now being further enhanced as a platform to build additional transgene traits for vaccines, altered protein composition, and to produce high levels of ß-carotene an intrinsic orange-colored aquafeed marker to distinguish the seeds from commodity beans and as the metabolic feedstock precursor of highly valued astaxanthin.

11.
PLoS One ; 11(6): e0157034, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27314851

RESUMEN

Necrotizing enterocolitis (NEC) is a devastating condition of premature infants that results from the gut microbiome invading immature intestinal tissues. This results in a life-threatening disease that is frequently treated with the surgical removal of diseased and dead tissues. Epidermal growth factor (EGF), typically found in bodily fluids, such as amniotic fluid, salvia and mother's breast milk, is an intestinotrophic growth factor and may reduce the onset of NEC in premature infants. We have produced human EGF in soybean seeds to levels biologically relevant and demonstrated its comparable activity to commercially available EGF. Transgenic soybean seeds expressing a seed-specific codon optimized gene encoding of the human EGF protein with an added ER signal tag at the N' terminal were produced. Seven independent lines were grown to homozygous and found to accumulate a range of 6.7 +/- 3.1 to 129.0 +/- 36.7 µg EGF/g of dry soybean seed. Proteomic and immunoblot analysis indicates that the inserted EGF is the same as the human EGF protein. Phosphorylation and immunohistochemical assays on the EGF receptor in HeLa cells indicate the EGF protein produced in soybean seed is bioactive and comparable to commercially available human EGF. This work demonstrates the feasibility of using soybean seeds as a biofactory to produce therapeutic agents in a soymilk delivery platform.


Asunto(s)
Enterocolitis Necrotizante/genética , Factor de Crecimiento Epidérmico/biosíntesis , Glycine max/genética , Plantas Modificadas Genéticamente/genética , Enterocolitis Necrotizante/patología , Factor de Crecimiento Epidérmico/administración & dosificación , Receptores ErbB/genética , Receptores ErbB/metabolismo , Microbioma Gastrointestinal/genética , Células HeLa , Humanos , Fosforilación , Semillas/genética
12.
Mol Plant Microbe Interact ; 15(12): 1213-8, 2002 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-12481993

RESUMEN

The syringolide receptor P34 mediates avrD-Rpg4 gene-for-gene complementarity in soybean. However, the mechanism underlying P34 signal transmission after syringolide binding is unknown. In an effort to identify a second messenger for P34, soybean leaf proteins were run though a P34-affinity column. A 42-kDa protein which specifically bound to the column was identified as a putative plant NADH-dependent hydroxypyruvate reductase (HPR) by N-terminal peptide sequencing. HPR is an important enzyme involved in the plant photorespiration system. Screening of a soybean cDNA library yielded two distinct HPR clones that encoded proteins with 97% identity (P42-1 and P42-2). Surprisingly, only P42-2 displayed good binding with P34 in a yeast two-hybrid assay, indicating that P42-2, but not P42-1, is a potential second messenger for P34. Glycerate and its analogs, which are utilized in the photorespiration system, were tested for their inhibitory effect on syringolide-induced hypersensitive response (HR) to evaluate the biological significance of P42-2. Interestingly, the downstream products of HPR (glycerate and 3-phosphoglycerate) inhibited HR but the upstream compounds (hydroxypyruvate or serine) did not have a significant effect on HR. These results suggest that P42-2 is a primary target for a P34/syringolide complex and that P42-2 binding with the complex probably induces HR by inhibiting one or more HPR functions in soybean.


Asunto(s)
Deshidrogenasas de Carbohidratos/metabolismo , Glycine max/genética , Proteínas de Plantas/metabolismo , Alérgenos , Secuencia de Aminoácidos , Antígenos de Plantas , Sitios de Unión/genética , Deshidrogenasas de Carbohidratos/genética , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , Luz , Datos de Secuencia Molecular , Consumo de Oxígeno/fisiología , Consumo de Oxígeno/efectos de la radiación , Proteínas de Plantas/genética , Unión Proteica , Saccharomyces cerevisiae/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Proteínas de Soja , Glycine max/enzimología , Técnicas del Sistema de Dos Híbridos
13.
Protist ; 155(1): 65-78, 2004 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15144059

RESUMEN

The peridinin-pigmented plastids of dinoflagellates are very poorly understood, in part because of the paucity of molecular data available from these endosymbiotic organelles. To identify additional gene sequences that would carry information about the biology of the peridinin-type dinoflagellate plastid and its evolutionary history, an analysis was undertaken of arbitrarily selected sequences from cDNA libraries constructed from Lingulodinium polyedrum (1012 non-redundant sequences) and Amphidinium carterae (2143). Among the two libraries 118 unique plastid-associated sequences were identified, including 30 (most from A. carterae) that are encoded in the plastid genome of the red alga Porphyra. These sequences probably represent bona fide nuclear genes, and suggest that there has been massive transfer of genes from the plastid to the nuclear genome in dinoflagellates. These data support the hypothesis that the peridinin-type plastid has a minimal genome, and provide data that contradict the hypothesis that there is an unidentified canonical genome in the peridinin-type plastid. Sequences were also identified that were probably transferred directly from the nuclear genome of the red algal endosymbiont, as well as others that are distinctive to the Alveolata. A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI.


Asunto(s)
Núcleo Celular/genética , Cloroplastos/genética , Dinoflagelados/genética , Etiquetas de Secuencia Expresada , Genes Protozoarios , Animales , ADN Complementario/química , ADN Complementario/aislamiento & purificación , ADN Protozoario/química , ADN Protozoario/aislamiento & purificación , Evolución Molecular , Biblioteca de Genes , Genómica , Datos de Secuencia Molecular , Recombinación Genética , Análisis de Secuencia de ADN
14.
Front Plant Sci ; 5: 437, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25232359

RESUMEN

The soybean seed's protein content and composition are regulated by both genetics and physiology. Overt seed protein content is specified by the genotype's genetic framework and is selectable as a breeding trait. Within the genotype-specified protein content phenotype soybeans have the capacity to rebalance protein composition to create differing proteomes. Soybeans possess a relatively standardized proteome, but mutation or targeted engineering can induce large-scale proteome rebalancing. Proteome rebalancing shows that the output traits of seed content and composition result from two major types of regulation: genotype and post-transcriptional control of the proteome composition. Understanding the underlying mechanisms that specifies the seed proteome can enable engineering new phenotypes for the production of a high-quality plant protein source for food, feed, and industrial proteins.

16.
Curr Opin Biotechnol ; 22(2): 224-30, 2011 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-21129947

RESUMEN

Concerns about food allergy and its societal growth are intertwined with the growing advances in plant biotechnology. The knowledge of plant genes and protein structures provides the key foundation to understanding biochemical processes that produce food allergy. Biotechnology offers the prospect of producing low-allergen or allergen null plants that could mitigate the allergic response. Modified low-IgE binding variants of allergens could be used as a vaccine to build immunotolerance in sensitive individuals. The potential to introduce new allergens into the food supply by biotechnology products is a regulatory concern.


Asunto(s)
Biotecnología/métodos , Hipersensibilidad a los Alimentos/prevención & control , Plantas Modificadas Genéticamente/metabolismo , Plantas/metabolismo , Alérgenos/genética , Alérgenos/metabolismo , Alimentos Modificados Genéticamente , Desarrollo de la Planta , Plantas/genética , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/crecimiento & desarrollo
17.
GM Crops ; 1(1): 2-7, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-21912205

RESUMEN

There are many diverse uses for industrial proteins with new opportunities for novel uses frequently emerging. Prominent among these uses are enzymes catalyzing the processing of food/feed and for the production of cellulosic biofuels. Other significant industrial protein uses include antibodies and other binding proteins for purification and/or clean-up of industrial product streams. Enabling technology is needed to produce these now expensive industrial proteins could be produced cost-effectively. Plant-based production of industrial enzymes offers the prospect of massive, scalable production, coupled with low production cost especially if a co-product, such as seed oil or starch, subsidizes the primary crop production costs. High-protein seeds whose composition is remodeled to produce industrial proteins can be a cost-effective means to produce industrial proteins. There are both technical and regulatory issues to resolve in order to deploy plants and seeds as industrial protein production platforms and many of these issues may be more easily resolved by developing nonfood crops specifically for use as industrial production platforms. An emerging industrial plant, Camelina, has potential as a protein-production platform subsidized by the seed oil co-product.


Asunto(s)
Brassicaceae/genética , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente/genética , Semillas/metabolismo , Biotecnología/tendencias , Brassicaceae/crecimiento & desarrollo , Brassicaceae/metabolismo , Productos Agrícolas/genética , Productos Agrícolas/crecimiento & desarrollo , Productos Agrícolas/metabolismo , Fuentes Generadoras de Energía , Aceites de Plantas/metabolismo , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente/crecimiento & desarrollo , Plantas Modificadas Genéticamente/metabolismo , Ingeniería de Proteínas/métodos , Ingeniería de Proteínas/tendencias , Semillas/genética , Semillas/crecimiento & desarrollo , Transformación Genética
18.
Curr Opin Plant Biol ; 11(6): 672-9, 2008 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-18824401

RESUMEN

Plant cells produce and accumulate insoluble triglycerides, proteins, and rubber that are assembled into inert, ER-derived organelles broadly termed as ER bodies. ER bodies appear to originate from tubular ER domains that are maintained by cytoskeletal interactions and integral ER proteins. ER bodies sequestering insoluble substances usually are transferred to the vacuole but sometimes remain as cytoplasmic organelles. Some otherwise soluble ER-synthesized proteins are converted to insoluble aggregates to produce ER bodies for transfer to the vacuole. This process constitutes an alternate secretory system to assemble and traffic transport-incompetent insoluble materials.


Asunto(s)
Retículo Endoplásmico/metabolismo , Cuerpos de Inclusión/metabolismo , Citoesqueleto/metabolismo , Aceites de Plantas/metabolismo , Proteínas de Plantas/metabolismo , Solubilidad
19.
Mol Plant ; 1(6): 910-24, 2008 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19825592

RESUMEN

Using RNAi, the seed oil body protein 24-kDa oleosin has been suppressed in transgenic soybeans. The endoplasmic reticulum (ER) forms micro-oil bodies about 50 nm in diameter that coalesce with adjacent oil bodies forming a hierarchy of oil body sizes. The oil bodies in the oleosin knockdown form large oil body-ER complexes with the interior dominated by micro-oil bodies and intermediate-sized oil bodies, while the peripheral areas of the complex are dominated by large oil bodies. The complex merges to form giant oil bodies with onset of seed dormancy that disrupts cell structure. The transcriptome of the oleosin knockdown shows few changes compared to wild-type. Proteomic analysis of the isolated oil bodies of the 24-kDa oleosin knockdown shows the absence of the 24-kDa oleosin and the presence of abundant caleosin and lipoxygenase. The formation of the micro-oil bodies in the oleosin knockdown is interpreted to indicate a function of the oleosin as a surfactant.


Asunto(s)
Retículo Endoplásmico/metabolismo , Glycine max/metabolismo , Cuerpos de Inclusión/metabolismo , Proteínas de la Membrana/metabolismo , Aceites de Plantas/metabolismo , Proteínas de Plantas/metabolismo , Supervivencia Celular , Cotiledón/citología , Cotiledón/metabolismo , Cotiledón/ultraestructura , Desecación , Regulación hacia Abajo/genética , Electroforesis en Gel Bidimensional , Electroforesis en Gel de Poliacrilamida , Retículo Endoplásmico/ultraestructura , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Técnicas de Silenciamiento del Gen , Germinación/fisiología , Cuerpos de Inclusión/ultraestructura , Espectrometría de Masas , Fenotipo , Proteoma , ARN Mensajero/genética , ARN Mensajero/metabolismo , Semillas/citología , Semillas/metabolismo , Glycine max/citología , Glycine max/genética , Glycine max/ultraestructura , Regulación hacia Arriba/genética
20.
Cryobiology ; 54(2): 154-63, 2007 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-17316598

RESUMEN

The suitability of using Arabidopsis as a model plant to investigate freezing tolerance was evaluated by observing similarities to winter cereals in tissue damage following controlled freezing and determining the extent to which Arabidopsis undergoes subzero-acclimation. Plants were grown and frozen under controlled conditions and percent survival was evaluated by observing re-growth after freezing. Paraffin embedded sections of plants were triple stained and observed under light microscopy. Histological observations of plants taken 1 week after freezing showed damage analogous to winter cereals in the vascular tissue of roots and leaf axels but no damage to meristematic regions. The LT(50) of non-acclimated Arabidopsis decreased from about -6 degrees C to a minimum of about -13 degrees C after 7 days of cold-acclimation at 3 degrees C. After exposing cold-acclimated plants to -3 degrees C for 3 days (subzero-acclimation) the LT(50) was lowered an additional 3 degrees C. Defining the underlying mechanisms of subzero-acclimation in Arabidopsis may provide an experimental platform to help understand winter hardiness in economically important crop species. However, distinctive histological differences in crown anatomy between Arabidopsis and winter cereals must be taken into account to avoid misleading conclusions on the nature of winter hardiness in winter cereals.


Asunto(s)
Aclimatación/fisiología , Arabidopsis/fisiología , Avena/fisiología , Frío , Arabidopsis/anatomía & histología , Avena/anatomía & histología
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