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Ocean acidification is predicted to have a wide range of impacts on fish, but there has been little focus on broad-ranging pelagic fish species. Early life stages of fish are thought to be particularly susceptible to CO2 exposure, since acid-base regulatory faculties may not be fully developed. We obtained yellowfin tuna (Thunnus albacares) from a captive spawning broodstock population and exposed them to control or 1900 µatm CO2 through the first three days of development as embryos transitioned into yolk sac larvae. Metabolic rate, yolk sac depletion, and oil globule depletion were measured to assess overall energy usage. To determine if CO2 altered protein catabolism, tissue nitrogen content and nitrogenous waste excretion were quantified. CO2 exposure did not significantly impact embryonic metabolic rate, yolk sac depletion, or oil globule depletion, however, there was a significant decrease in metabolic rate at the latest measured yolk sac larval stage (36 h post fertilization). CO2-exposure led to a significant increase in nitrogenous waste excretion in larvae, but there were no differences in nitrogen tissue accumulation. Nitrogenous waste accumulated in embryos as they developed but decreased after hatch, coinciding with a large increase in nitrogenous waste excretion and increased metabolic rate in newly hatched larvae. Our results provide insight into how yellowfin tuna are impacted by increases in CO2 in early development, but more research with higher levels of replication is needed to better understand long-term impacts and acid-base regulatory mechanisms in this important pelagic fish.
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Dióxido de Carbono , Atún , Animales , Atún/metabolismo , Dióxido de Carbono/metabolismo , Concentración de Iones de Hidrógeno , Agua de Mar , LarvaRESUMEN
The Deepwater Horizon (DWH) disaster released 3.19 million barrels of crude oil into the Gulf of Mexico (GOM) in 2010, overlapping the habitat of pelagic fish populations. Using mahi-mahi (Coryphaena hippurus)âa highly migratory marine teleost present in the GOM during the spillâas a model species, laboratory experiments demonstrate injuries to physiology and behavior following oil exposure. However, more than a decade postspill, impacts on wild populations remain unknown. To address this gap, we exposed wild mahi-mahi to crude oil or control conditions onboard a research vessel, collected fin clip samples, and tagged them with electronic tags prior to release into the GOM. We demonstrate profound effects on survival and reproduction in the wild. In addition to significant changes in gene expression profiles and predation mortality, we documented altered acceleration and habitat use in the first 8 days oil-exposed individuals were at liberty as well as a cessation of apparent spawning activity for at least 37 days. These data reveal that even a brief and low-dose exposure to crude oil impairs fitness in wild mahi-mahi. These findings offer new perspectives on the lasting impacts of the DWH blowout and provide insight about the impacts of future deep-sea oil spills.
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Perciformes , Contaminación por Petróleo , Petróleo , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Animales , Golfo de México , Petróleo/metabolismo , Hidrocarburos Policíclicos Aromáticos/análisis , Contaminantes Químicos del Agua/metabolismoRESUMEN
Teleost fishes are diverse and successful, comprising almost half of all extant vertebrate species. It has been suggested that their success as a group is related, in part, to their unique O2 transport system, which includes pH-sensitive hemoglobin, a red blood cell ß-adrenergic Na+/H+ exchanger (RBC ß-NHE) that protects red blood cell pH, and plasma accessible carbonic anhydrase which is absent at the gills but present in some tissues, that short-circuits the ß-NHE to enhance O2 unloading during periods of stress. However, direct support for this has only been examined in a few species of salmonids. Here, we expand the knowledge of this system to two warm-water, highly active marine percomorph fish, cobia (Rachycentron canadum) and mahi-mahi (Coryphaena hippurus). We show evidence for RBC ß-NHE activity in both species, and characterize the Hb-O2 transport system in one of those species, cobia. We found significant RBC swelling following ß-adrenergic stimulation in both species, providing evidence for the presence of a rapid, active RBC ß-NHE in both cobia and mahi-mahi, with a time-course similar to that of salmonids. We generated oxygen equilibrium curves (OECs) for cobia blood and determined the P50, Hill, and Bohr coefficients, and used these data to model the potential for enhanced O2 unloading. We determined that there was potential for up to a 61% increase in O2 unloading associated with RBC ß-NHE short-circuiting, assuming a - 0.2 ∆pHa-v in the blood. Thus, despite phylogenetic and life history differences between cobia and the salmonids, we found few differences between their Hb-O2 transport systems, suggesting conservation of this physiological trait across diverse teleost taxa.
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Peces/fisiología , Oxígeno/fisiología , Perciformes/fisiología , Animales , Eritrocitos/metabolismo , Proteínas de Peces/metabolismo , Peces/sangre , Hemoglobinas/metabolismo , Cinética , Oxígeno/sangre , Perciformes/sangre , Salmonidae/sangre , Salmonidae/fisiología , Especificidad de la EspecieRESUMEN
Estuarine crocodiles, Crocodylus porosus, inhabit freshwater, estuarine and marine environments. Despite being known to undertake extensive movements throughout and between hypo-osmotic and hyperosmotic environments, little is known about the role of the cloaca in coping with changes in salinity. We report here that, in addition to the well-documented functional plasticity of the lingual salt glands, the middle of the three cloacal segments (i.e. the urodaeum) responds to increased ambient salinity to enhance solute-coupled water absorption. This post-renal modification of urine serves to conserve water when exposed to hyperosmotic environments and, in conjunction with lingual salt gland secretions, enables C. porosus to maintain salt and water balance and thereby thrive in hyperosmotic environments. Isolated epithelia from the urodaeum of 70% seawater-acclimated C. porosus had a strongly enhanced short-circuit current (an indicator of active ion transport) compared with freshwater-acclimated crocodiles. This enhanced active ion absorption was driven by increased Na+/K+-ATPase activity, and possibly enhanced proton pump activity, and was facilitated by the apical epithelial Na+ channel (ENaC) and/or the apical Na+/H+ exchanger (NHE2), both of which are expressed in the urodaeum. NHE3 was expressed at very low levels in the urodaeum and probably does not contribute to solute-coupled water absorption in this cloacal segment. As C. porosus does not appear to drink water of salinities above 18 ppt, observations of elevated short-circuit current in the rectum as well as a trend for increased NHE2 expression in the oesophagus, the anterior intestine and the rectum suggest that dietary salt intake may stimulate salt and possibly water absorption by the gastrointestinal tract of C. porosus living in hyperosmotic environments.
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Caimanes y Cocodrilos/fisiología , Cloaca/metabolismo , Recto/metabolismo , Salinidad , Aclimatación/fisiología , Caimanes y Cocodrilos/metabolismo , Animales , Canales Epiteliales de Sodio/genética , Canales Epiteliales de Sodio/metabolismo , Transporte Iónico/fisiología , Masculino , Intercambiadores de Sodio-Hidrógeno/genética , Intercambiadores de Sodio-Hidrógeno/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/genética , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Orina/químicaRESUMEN
Publicly available toxicological studies on wastewaters associated with unconventional oil and gas (UOG) activities in offshore regions are nonexistent. The current study investigated the impact of hydraulic fracturing-generated flowback water (HF-FW) on whole organism swimming performance/respiration and cardiomyocyte contractility dynamics in mahi-mahi (Coryphaena hippurus-hereafter referred to as "mahi"), an organism which inhabits marine ecosystems where offshore hydraulic fracturing activity is intensifying. Following exposure to 2.75% HF-FW for 24 h, mahi displayed significantly reduced critical swimming speeds (Ucrit) and aerobic scopes (reductions of â¼40 and 61%, respectively) compared to control fish. Additionally, cardiomyocyte exposures to the same HF-FW sample at 2% dilutions reduced a multitude of mahi sarcomere contraction properties at various stimulation frequencies compared to all other treatment groups, including an approximate 40% decrease in sarcomere contraction size and a nearly 50% reduction in sarcomere relaxation velocity compared to controls. An approximate 8-fold change in expression of the cardiac contractile regulatory gene cmlc2 was also seen in ventricles from 2.75% HF-FW-exposed mahi. These results collectively identify cardiac function as a target for HF-FW toxicity and provide some of the first published data on UOG toxicity in a marine species.
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Fracking Hidráulico , Perciformes , Contaminación por Petróleo , Petróleo , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Animales , Ecosistema , Miocitos Cardíacos , Contaminación por Petróleo/análisis , Natación , Aguas Residuales , Agua , Contaminantes Químicos del Agua/toxicidadRESUMEN
Deepwater Horizon crude oil is comprised of polycyclic aromatic hydrocarbons that cause a number of cardiotoxic effects in marine fishes across all levels of biological organization and at different life stages. Although cardiotoxic impacts have been widely reported, the mechanisms underlying these impairments in adult fish remain understudied. In this study, we examined the impacts of crude oil on cardiomyocyte contractility and electrophysiological parameters in freshly isolated ventricular cardiomyocytes from adult mahi-mahi (Coryphaena hippurus). Cardiomyocytes directly exposed to oil exhibited reduced contractility over a range of environmentally relevant concentrations (2.8-12.9 µg l-1∑PAH). This reduction in contractility was most pronounced at higher stimulation frequencies, corresponding to the upper limits of previously measured in situ mahi heart rates. To better understand the mechanisms underlying impaired contractile function, electrophysiological studies were performed, which revealed oil exposure prolonged cardiomyocyte action potentials and disrupted potassium cycling (9.9-30.4 µg l-1∑PAH). This study is the first to measure cellular contractility in oil-exposed cardiomyocytes from a pelagic fish. Results from this study contribute to previously observed impairments to heart function and whole-animal exercise performance in mahi, underscoring the advantages of using an integrative approach in examining mechanisms of oil-induced cardiotoxicity in marine fish.
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Perciformes , Contaminación por Petróleo , Petróleo , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , AnimalesRESUMEN
Aqueous crude oil spills expose fish to varying concentrations of dissolved polycyclic aromatic hydrocarbons (PAHs), which can have lethal and sublethal effects. The heart is particularly vulnerable in early life stages, as PAH toxicity causes developmental cardiac abnormalities and impaired cardiovascular function. However, cardiac responses of juvenile and adult fish to acute oil exposure remain poorly understood. We sought to assess cardiac function in a pelagic fish species, the cobia (Rachycentron canadum), following acute (24 h) exposure to two ecologically relevant levels of dissolved PAHs. Cardiac power output (CPO) was used to quantify cardiovascular performance using an in situ heart preparation. Cardiovascular performance was varied using multiple concentrations of the ß-adrenoceptor agonist isoproterenol (ISO) and by varying afterload pressures. Oil exposure adversely affected CPO with control fish achieving maximum CPO's (4 mW g-1 Mv) greater than that of oil-exposed fish (1 mW g-1 Mv) at ISO concentrations of 1 × 10-6 M. However, the highest concentration of ISO (1 × 10-5 M) rescued cardiac function. This indicates an interactive effect between oil-exposure and ß-adrenergic stimulation and suggests if animals achieve very large increases in ß-adrenergic stimulation it could play a compensatory role that may mitigate some adverse effects of oil-exposure in vivo.
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Adrenérgicos , Corazón/efectos de los fármacos , Contaminación por Petróleo , Hidrocarburos Policíclicos Aromáticos , Animales , Peces , Perciformes , Petróleo , Contaminantes Químicos del AguaRESUMEN
Most fish studied to date efficiently compensate for a hypercapnic acid-base disturbance; however, many recent studies examining the effects of ocean acidification on fish have documented impacts at CO2 levels predicted to occur before the end of this century. Notable impacts on neurosensory and behavioral endpoints, otolith growth, mitochondrial function, and metabolic rate demonstrate an unexpected sensitivity to current-day and near-future CO2 levels. Most explanations for these effects seem to center on increases in Pco2 and HCO3- that occur in the body during pH compensation for acid-base balance; however, few studies have measured these parameters at environmentally relevant CO2 levels or directly related them to reported negative endpoints. This compensatory response is well documented, but noted variation in dynamic regulation of acid-base transport pathways across species, exposure levels, and exposure duration suggests that multiple strategies may be utilized to cope with hypercapnia. Understanding this regulation and changes in ion gradients in extracellular and intracellular compartments during CO2 exposure could provide a basis for predicting sensitivity and explaining interspecies variation. Based on analysis of the existing literature, the present review presents a clear message that ocean acidification may cause significant effects on fish across multiple physiological systems, suggesting that pH compensation does not necessarily confer tolerance as downstream consequences and tradeoffs occur. It remains difficult to assess if acclimation responses during abrupt CO2 exposures will translate to fitness impacts over longer timescales. Nonetheless, identifying mechanisms and processes that may be subject to selective pressure could be one of many important components of assessing adaptive capacity.
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Dióxido de Carbono/química , Peces/fisiología , Agua de Mar/química , Animales , Concentración de Iones de Hidrógeno , Océanos y MaresRESUMEN
Rising CO2 emissions have heightened the necessity for increased understanding of Earth's carbon cycle to predict future climates. The involvement of marine planktonic species in the global carbon cycle has been extensively studied, but contributions by marine fish remain poorly characterized. Marine teleost fishes produce carbonate minerals ('ichthyocarbonates') within the lumen of their intestines which are excreted at significant rates on a global scale. However, we have limited understanding of the fate of excreted ichthyocarbonate. We analyzed ichthyocarbonate produced by three different marine teleosts for mol%MgCO3 content, size, specific gravity, and dissolution rate to gain a better understanding of ichthyocarbonate fate. Based on the species examined here, we report that 75 % of ichthyocarbonates are ≤0.91 mm in diameter. Analyses indicate high Mg2+ content across species (22.3 to 32.3 % mol%MgCO3), consistent with previous findings. Furthermore, ichthyocarbonate specific gravity ranged from 1.23 to 1.33 g/cm3, and ichthyocarbonate dissolution rates varied among species as a function of aragonite saturation state. Ichthyocarbonate sinking rates and dissolution depth were estimated for the Atlantic, Pacific, and Indian ocean basins for the three species examined. In the North Atlantic, for example, ~33 % of examined ichthyocarbonates are expected to reach depths exceeding 200 m prior to complete dissolution. The remaining ~66 % of ichthyocarbonate is estimated to dissolve and contribute to shallow water alkalinity budgets. Considering fish biomass and ichthyocarbonate production rates, our results support that marine fishes are critical to the global carbon cycle, contributing to oceanic alkalinity budgets and thereby influencing the ability of the oceans to neutralize atmospheric CO2.
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Dióxido de Carbono , Ecosistema , Animales , Dióxido de Carbono/análisis , Gravedad Específica , Océanos y Mares , Carbonatos , Peces , Ciclo del Carbono , Océano Índico , Agua de Mar , CarbonoRESUMEN
Cardiovascular diseases are a rampant public health threat. Environmental contaminants, such as Cadmium (Cd), a toxic metal, have been linked to increased risk for cardiovascular diseases. Given that human exposure to Cd is increasing overtime, there is a need to develop new therapies to ameliorate Cd toxicity. Selenium (Se), an essential trace element, has been proposed to rescue the effects of Cd toxicity, with mixed effects. Se's narrow therapeutic window necessitates precise dosing to avoid toxicity. Here, we assessed the effects of various waterborne Cd and Se concentrations and sequences on cardiac function using zebrafish (Danio rerio). We showed that Cd induced pericardial edemas and modified heart rates in a concentration-dependent manner. To identify the therapeutic range of Se for Cd-induced cardiotoxicity, zebrafish embryos were treated with 0, 10, 50, 100, 150, or 200 µg/L Se for 1-4 days prior to exposure to Cd at 2.5, and 5 µg/L. We found that a 50 µg/L Se pre-treatment prior to Cd at 2.5 µg/L, but not at 5 µg/L, reduced the prevalence of pericardial edemas and ameliorated Cd-induced bradycardia in zebrafish. Embryos exposed to 10 and 50 µg/L of Se showed typical heart morphology, whereas other Se-exposed and Se-deficient fish presented pericardial edemas. Longer Se pre-treatment durations led to fewer incidences of pericardial edemas. Overall, this study highlights the importance of optimizing Se concentration and pre-treatment periods to harness its protective effects against Cd-induced cardiotoxicity. These findings provide insights into potential therapeutic strategies for reducing Cd-related cardiovascular damage in humans.
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Marine bony fish are important participants in Earth's carbon cycle through their contributions to the biological pump and the marine inorganic carbon cycle. However, uncertainties in the composition and magnitude of fish contributions preclude their integration into fully coupled carbon-climate models. Here, we consider recent upwards revisions to global fish biomass estimates (2.7-9.5×) and provide new stable carbon isotope measurements that show marine fish are prodigious producers of carbonate with unique composition. Assuming the median increase (4.17×) in fish biomass estimates is linearly reflected in fish carbonate (ichthyocarbonate) production rate, marine fish are estimated to produce between 1.43 and 3.99 Pg CaCO3 yr-1, but potentially as much as 9.03 Pg CaCO3 yr-1. Thus, marine fish carbonate production is equivalent to or potentially higher than contributions by coccolithophores or pelagic foraminifera. New stable carbon isotope analyses indicate that a significant proportion of ichthyocarbonate is derived from dietary carbon, rather than seawater dissolved inorganic carbon. Using a statistical mixing model to derive source contributions, we estimate ichthyocarbonate contains up to 81 % dietary carbon, with average compositions of 28-56 %, standing in contrast to contents <10 % in other biogenic carbonate minerals. Results also indicate ichthyocarbonate contains 5.5-40.4 % total organic carbon. When scaled to the median revised global production of ichthyocarbonate, an additional 0.08 to 1.61 Pg C yr-1 can potentially be added to estimates of fish contributions to the biological pump, significantly increasing marine fish contributions to total surface carbon export. Our integration of geochemical and physiological analyses identifies an overlooked link between carbonate production and the biological pump. Since ichthyocarbonate production is anticipated to increase with climate change scenarios, due to ocean warming and acidification, these results emphasize the importance of quantitative understanding of the multifaceted role of marine fish in the global carbon cycle.
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Carbono , Carbonatos , Animales , Humanos , Carbono/metabolismo , Carbonatos/química , Agua de Mar/química , Isótopos de Carbono/metabolismo , Dióxido de Carbono/metabolismo , Peces/metabolismo , Ciclo del Carbono , Proteínas de Transporte de Membrana/metabolismo , Océanos y MaresRESUMEN
Pelagic fish embryos are thought to float in or near surface waters for the majority of their development and are presumed to have little to no control over their mobility, rendering these embryos at high risk for damages associated with surface stressors such as ultraviolet radiation (UVR). We recently challenged these long-standing paradigms by characterizing a potential mechanism of stressor avoidance in early-life stage mahi-mahi (Coryphaena hippurus) in which embryos sense external cues, such as UVR, and modify their buoyancy to reduce further exposure. It is unknown whether embryos of other marine fish with pelagic spawning strategies have similar capabilities. To fill this knowledge gap, we investigated buoyancy change in response to UVR in three additional species of marine fish that utilize a pelagic spawning strategy: yellowfin tuna (Thunnus albacares), red snapper (Lutjanus campechanus), and cobia (Rachycentron canadum). Embryos of all three species displayed increased specific gravity and loss of buoyancy after exposures to environmentally relevant doses of UVR, a response that may be ubiquitous to fish with pelagic embryos. To gain further insight into this response, we investigated recovery of buoyancy, oxygen consumption, energy depletion, and photolyase induction in response to UVR exposures in at least one of the three species listed above.
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Perciformes , Contaminación por Petróleo , Petróleo , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Animales , Embrión no Mamífero/química , Hidrocarburos Policíclicos Aromáticos/análisis , Rayos UltravioletaRESUMEN
Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive Mg2+ load from the environment with an equal excretion. However, the molecular transporters involved in Mg2+ secretion are poorly understood. We investigated whether environmental MgCl2 alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal Mg2+ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the Na+/K+-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol L-1 ambient [Mg2+], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [Mg2+] was explored by exposing toadfish to control (50 mmol L-1 Mg2+), or elevated [Mg2+] (100 mmol L-1) at a constant salinity for 7 days. Mg2+ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In Mg2+-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [Mg2+], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal Mg2+ transporters is induced by elevated [Mg2+] in combination with salinity rather than elevated ambient [Mg2+] alone.
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Batrachoidiformes , Animales , Batrachoidiformes/metabolismo , Branquias/metabolismo , Riñón/metabolismo , Magnesio/metabolismo , Osmorregulación , Salinidad , Sodio/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/genética , ATPasa Intercambiadora de Sodio-Potasio/metabolismoRESUMEN
Increases in ambient salinity demand parallel increases in intestinal base secretion for maintenance of osmoregulatory status, which is likely the cause of a transient acidosis following transfer of euryhaline fish from freshwater to seawater. It was predicted that transfer of the marine Gulf toadfish (Opsanus beta) from seawater (35 ppt) to hypersaline (60 ppt) seawater (HSW) would lead to a transient acidosis that would be compensated by increases in branchial acid excretion to offset the acid-base disturbance. Toadfish exposed to HSW showed a significant decrease in blood pH and [HCO3-] but no increase in pCO2, followed by a full recovery after 48-96 h. A similar metabolic acidosis and recovery was found when fish were exposed to 60-ppt HCO3--free seawater (HEPES-buffered), which may suggest that compensation for intestinal base loss during hypersaline treatment is from gill H+ excretion rather than gill HCO3- uptake. However, we cannot rule out that reduced branchial HCO3- excretion contributed to an increase in net acid excretion. Since colchicine prevents full compensation, translocation of H+ and/or HCO3- transporters between cytosolic compartments and plasma membrane fractions might be involved in compensating for the hypersalinity-induced acidosis. Translocation of transporters rather than de novo synthesis may represent a faster and less energetically demanding response to rapidly fluctuating and high salinities encountered by toadfish in their natural environment.
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Acidosis , Batrachoidiformes , Acidosis/veterinaria , Animales , Transporte Biológico , Branquias , Salinidad , Agua de Mar , AguaRESUMEN
An understudied consequence of coastal urbanization on marine environments is sound pollution. While underwater anthropogenic sounds are recognized as a threat to aquatic organisms, little is known about the effects of above-surface coastal sound pollution on adjacent underwater soundscapes and the organisms inhabiting them. Here, the impact of noise from the 2019 Ultra Music Festival® in Miami, FL, USA was assessed at the University of Miami Experimental Hatchery (UMEH) located directly adjacent to the music festival and on underwater sound levels in Bear Cut, a nearby water channel. In addition, stress hormone levels in fish held at UMEH were measured before and during the festival. Air sound levels recorded at UMEH during the Ultra Music Festival did not exceed 72 dBA and 98 dBC. The subsurface sound intensity levels in the low frequency band increased by 2-3 dB re 1 µPa in the adjacent waterway, Bear Cut, and by 7-9 dB re 1 µPa in the fish tanks at UMEH. Gulf toadfish (Opsanus beta) housed in the UMEH tanks experienced a 4-5 fold increase in plasma cortisol, their main stress hormone, during the first night of the Ultra Music Festival compared to two baseline samples taken 3 weeks and 4 days before Ultra. While this study offers preliminary insights into this type of sound pollution, more research is needed to conclude if Ultra caused a stress response in wild organisms and to fully understand the implications of this type of sound pollution.
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Música , Animales , Peces , Vacaciones y Feriados , Ruido , SonidoRESUMEN
Behavioural impairment following exposure to ocean acidification-relevant CO2 levels has been noted in a broad array of taxa. The underlying cause of these disruptions is thought to stem from alterations of ion gradients ( HC O 3 - / C l - ) across neuronal cell membranes that occur as a consequence of maintaining pH homeostasis via the accumulation of HC O 3 - . While behavioural impacts are widely documented, few studies have measured acid-base parameters in species showing behavioural disruptions. In addition, current studies examining mechanisms lack resolution in targeting specific neural pathways corresponding to a given behaviour. With these considerations in mind, acid-base parameters and behaviour were measured in a model organism used for decades as a research model to study learning, the California sea hare (Aplysia californica). Aplysia exposed to elevated CO2 increased haemolymph HC O 3 - , achieving full and partial pH compensation at 1200 and 3000 µatm CO2, respectively. Increased CO2 did not affect self-righting behaviour. In contrast, both levels of elevated CO2 reduced the time of the tail-withdrawal reflex, suggesting a reduction in antipredator response. Overall, these results confirm that Aplysia are promising models to examine mechanisms underlying CO2-induced behavioural disruptions since they regulate HC O 3 - and have behaviours linked to neural networks amenable to electrophysiological testing.
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Mitochondrial function is critical to support aerobic metabolism through the production of ATP, and deficiencies in mitochondrial bioenergetics will directly impact the performance capacity of highly aerobic tissues such as the myocardium. Cardiac function in fish has been shown to be negatively affected by crude oil exposure, however, the mechanism for this adverse response is largely unexplored. We hypothesized that lipophilic polycyclic aromatic hydrocarbons (PAHs) found in crude oil disrupt the electron transport system (ETS) ultimately leading to mitochondrial dysfunction. In this study, mitochondrial respiration and ADP affinity we measured using high resolution respirometery in permeabilized cardiac muscle fibers of young adult Mahi-mahi (Coryphaena hippurus) after an acute (24â¯h) whole animal crude oil exposure. Oil exposure reduced both complex I-fueled ADP stimulated respiration (OXPHOSCI) and complex I,II-fueled ADP stimulated respiration (OXPHOSCI, CII) by 33%,while complex II-fueled ADP stimulated respiration (OXPHOSCII) was reduced by 25%. These changes were found without changes in enzyme activity or mitochondrial density between control and oil exposed Mahi. Additionally, mitochondrial affinity for ADP was decreased three-fold after acute exposure to crude oil. We purpose that acute crude oil exposure selectively impairs mitochondrial complexes of the electron transport system and ATP supply to the cell. This limited ATP supply could present several challenges to a predatory animal like the mahi; including a reliance on anaerobic metabolism and ultimately cell or tissue death as metabolic substrates are rapidly depleted. However, the impact of this impairment may only be evident under periods of increased aerobic metabolic demand.
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Adenosina Difosfato/metabolismo , Mitocondrias/efectos de los fármacos , Miocitos Cardíacos/efectos de los fármacos , Perciformes , Petróleo/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Mitocondrias/enzimología , Miocitos Cardíacos/metabolismo , Consumo de Oxígeno/efectos de los fármacosRESUMEN
Aerobic exercise capacity is dependent on the cardiorespiratory system's ability to supply oxygen at a rate that meets energetic demands. In teleost fish crude oil exposure, with the associated polycyclic aromatic hydrocarbons (PAH's), reduces exercise performance and this has been hypothesized to be due to compromised cardiovascular function. In this study, we test this hypothesis by simultaneously measuring cardiovascular performance, oxygen consumption, and swim performance in a pelagic teleost, the cobia (Rachycentron canadum). Metabolic rate increased over 300% in both groups during the swim trial but as the fish approached the critical swim speed (Ucrit) MO2 was 12% lower in the oil exposed fish. Further, stroke volume was initially 35% lower while heart rate was 15% higher in the oil exposed compared to control fish. Our findings suggested, while aspects of cardiovascular and metabolic function are altered by oil exposure, additional studies are needed to further understand the homeostatic mechanisms that may sustain cardiovascular function at higher exercise intensities in cobia.
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Peces/fisiología , Consumo de Oxígeno/efectos de los fármacos , Petróleo/toxicidad , Condicionamiento Físico Animal , AnimalesRESUMEN
Energetic costs associated with ion and acid-base regulation in response to ocean acidification have been predicted to decrease the energy available to fish for basic life processes. However, the low cost of ion regulation (6-15% of standard metabolic rate) and inherent variation associated with whole-animal metabolic rate measurements have made it difficult to consistently demonstrate such a cost. Here we aimed to gain resolution in assessing the energetic demand associated with acid-base regulation by examining ion movement and O2 consumption rates of isolated intestinal tissue from Gulf toadfish acclimated to control or 1900 µatm CO2 (projected for year 2300). The active marine fish intestine absorbs ions from ingested seawater in exchange for HCO3- to maintain water balance. We demonstrate that CO2 exposure causes a 13% increase of intestinal HCO3- secretion that the animal does not appear to regulate. Isolated tissue from CO2-exposed toadfish also exhibited an 8% higher O2 consumption rate than tissue from controls. These findings show that compensation for CO2 leads to a seemingly maladaptive persistent base (HCO3-) loss that incurs an energetic expense at the tissue level. Sustained increases to baseline metabolic rate could lead to energetic reallocations away from other life processes at the whole-animal level.