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1.
Nat Methods ; 21(1): 132-141, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-38129618

RESUMEN

Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue and has transformed neural imaging, but applying this technique in vivo can be limited by the mechanical and optical constraints of conventional objectives. Short working distance objectives can collide with compact surgical windows or other instrumentation and preclude imaging. Here we present an ultra-long working distance (20 mm) air objective called the Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a more than 4 mm2 field of view with submicrometer lateral resolution and is compatible with commonly used multiphoton imaging systems. A novel mechanical design, wider than typical microscope objectives, enabled this combination of specifications. We share the full optical prescription, and report performance including in vivo two-photon and three-photon imaging in an array of species and preparations, including nonhuman primates. The Cousa objective can enable a range of experiments in neuroscience and beyond.


Asunto(s)
Colorantes , Microscopía de Fluorescencia por Excitación Multifotónica , Animales , Microscopía de Fluorescencia por Excitación Multifotónica/métodos
2.
Cereb Cortex ; 34(2)2024 01 31.
Artículo en Inglés | MEDLINE | ID: mdl-38365273

RESUMEN

Traumatic brain injury (TBI) is the leading cause of death in young people and can cause cognitive and motor dysfunction and disruptions in functional connectivity between brain regions. In human TBI patients and rodent models of TBI, functional connectivity is decreased after injury. Recovery of connectivity after TBI is associated with improved cognition and memory, suggesting an important link between connectivity and functional outcome. We examined widespread alterations in functional connectivity following TBI using simultaneous widefield mesoscale GCaMP7c calcium imaging and electrocorticography (ECoG) in mice injured using the controlled cortical impact (CCI) model of TBI. Combining CCI with widefield cortical imaging provides us with unprecedented access to characterize network connectivity changes throughout the entire injured cortex over time. Our data demonstrate that CCI profoundly disrupts functional connectivity immediately after injury, followed by partial recovery over 3 weeks. Examining discrete periods of locomotion and stillness reveals that CCI alters functional connectivity and reduces theta power only during periods of behavioral stillness. Together, these findings demonstrate that TBI causes dynamic, behavioral state-dependent changes in functional connectivity and ECoG activity across the cortex.


Asunto(s)
Lesiones Traumáticas del Encéfalo , Lesiones Encefálicas , Humanos , Ratones , Animales , Adolescente , Lesiones Traumáticas del Encéfalo/complicaciones , Lesiones Traumáticas del Encéfalo/diagnóstico por imagen , Modelos Animales de Enfermedad , Corteza Cerebral/diagnóstico por imagen , Cognición
3.
Nat Rev Neurosci ; 20(5): 272-281, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-30837689

RESUMEN

Cellular mechanisms that regulate the interplay of synaptic excitation and inhibition are thought to be central to the functional stability of healthy neuronal circuits. A growing body of literature demonstrates the capacity for inhibitory GABAergic synapses to exhibit long-term plasticity in response to changes in neuronal activity. Here, we review this expanding field of research, focusing on the diversity of mechanisms that link glutamatergic signalling, postsynaptic action potentials and inhibitory synaptic strength. Several lines of evidence indicate that multiple, parallel forms of plasticity serve to regulate activity at both the input and output domains of individual neurons. Overall, these varied phenomena serve to promote both stability and flexibility over the life of the organism.


Asunto(s)
Potenciales de Acción/fisiología , Encéfalo/fisiología , Neuronas GABAérgicas/fisiología , Inhibición Neural/fisiología , Plasticidad Neuronal/fisiología , Sinapsis/fisiología , Animales
4.
Nat Methods ; 17(1): 107-113, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31686040

RESUMEN

Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian cortex, but technical challenges have limited conceptual links between the function of local neuronal circuits and brain-wide network dynamics. We present a method for simultaneous cellular-resolution two-photon calcium imaging of a local microcircuit and mesoscopic widefield calcium imaging of the entire cortical mantle in awake mice. Our multi-scale approach involves a microscope with an orthogonal axis design where the mesoscopic objective is oriented above the brain and the two-photon objective is oriented horizontally, with imaging performed through a microprism. We also introduce a viral transduction method for robust and widespread gene delivery in the mouse brain. These approaches allow us to identify the behavioral state-dependent functional connectivity of pyramidal neurons and vasoactive intestinal peptide-expressing interneurons with long-range cortical networks. Our imaging system provides a powerful strategy for investigating cortical architecture across a wide range of spatial scales.


Asunto(s)
Encéfalo/fisiología , Calcio/metabolismo , Corteza Cerebral/fisiología , Red Nerviosa/fisiología , Neuroimagen/métodos , Neuronas/fisiología , Fotones , Animales , Conducta Animal , Encéfalo/citología , Corteza Cerebral/citología , Interneuronas/citología , Interneuronas/fisiología , Ratones , Neuronas/citología , Células Piramidales/citología , Células Piramidales/fisiología , Péptido Intestinal Vasoactivo/metabolismo
5.
Nat Methods ; 17(12): 1262-1271, 2020 12.
Artículo en Inglés | MEDLINE | ID: mdl-33139894

RESUMEN

Achieving a comprehensive understanding of brain function requires multiple imaging modalities with complementary strengths. We present an approach for concurrent widefield optical and functional magnetic resonance imaging. By merging these modalities, we can simultaneously acquire whole-brain blood-oxygen-level-dependent (BOLD) and whole-cortex calcium-sensitive fluorescent measures of brain activity. In a transgenic murine model, we show that calcium predicts the BOLD signal, using a model that optimizes a gamma-variant transfer function. We find consistent predictions across the cortex, which are best at low frequency (0.009-0.08 Hz). Furthermore, we show that the relationship between modality connectivity strengths varies by region. Our approach links cell-type-specific optical measurements of activity to the most widely used method for assessing human brain function.


Asunto(s)
Mapeo Encefálico/métodos , Proteínas de Unión al Calcio/metabolismo , Corteza Cerebral/diagnóstico por imagen , Imagen por Resonancia Magnética/métodos , Animales , Análisis de los Gases de la Sangre , Calcio/metabolismo , Proteínas de Unión al Calcio/genética , Fluorescencia , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Ratones , Ratones Transgénicos , Oxígeno/análisis
6.
J Neurosci ; 40(4): 720-725, 2020 01 22.
Artículo en Inglés | MEDLINE | ID: mdl-31969490

RESUMEN

ACh is a signaling molecule in the mammalian CNS, with well-documented influence over cognition and behavior. However, the nature of cholinergic signaling in the brain remains controversial, with ongoing debates focused on the spatial and temporal resolution of ACh activity. Generally, opposing views have embraced a dichotomy between transmission as slow and volume-mediated versus fast and synaptic. Here, we provide the perspective that ACh, like most other neurotransmitters, exhibits both fast and slow modes that are strongly determined by the anatomy of cholinergic fibers, the distribution and the signaling mechanisms of receptor subtypes, and the dynamics of ACh hydrolysis. Current methodological approaches remain limited in their ability to provide detailed analyses of these underlying factors. However, we believe that the continued development of novel technologies in combination with a more nuanced view of cholinergic activity will open critical new avenues to a better understanding of ACh in the brain.Dual Perspectives Companion Paper: Forebrain Cholinergic Signaling: Wired and Phasic, Not Tonic, and Causing Behavior, by Martin Sarter and Cindy Lustig.


Asunto(s)
Fibras Colinérgicas/fisiología , Neocórtex/fisiología , Transmisión Sináptica/fisiología , Animales , Transducción de Señal/fisiología
8.
Nat Rev Neurosci ; 15(9): 567-72, 2014 09.
Artículo en Inglés | MEDLINE | ID: mdl-25116141

RESUMEN

Neuronal circuits are defined by synaptic connections between their cellular constituents. In this article, I highlight several recent studies emphasizing the surprising level of precision exhibited by inhibitory GABAergic synapses within the neocortex and hippocampus. Specifically, GABAergic inputs to dendritic shafts and spines of pyramidal cells have a key role in the localized regulation of neuronal Ca(2+) signalling. These findings provide important new insights into the cellular mechanisms underlying the contributions of inhibitory transmission to both normal and abnormal brain activity.


Asunto(s)
Señalización del Calcio/fisiología , Calcio/metabolismo , Dendritas/fisiología , Neuronas GABAérgicas/citología , Inhibición Neural/fisiología , Animales , Red Nerviosa/fisiología
9.
J Physiol ; 596(3): 515-534, 2018 02 01.
Artículo en Inglés | MEDLINE | ID: mdl-29265375

RESUMEN

KEY POINTS: Rodents explore their immediate environment using their whiskers. Such exploration leads to micromotions, which contain many high-frequency (50-200 Hz) components. High-frequency whisker motion is represented faithfully in the temporal structure of the spike trains of trigeminal neurons. However, the representation of high-frequency sensory inputs in cortex is not fully understood. By combining extracellular and intracellular recordings in the rat somatosensory cortex and thalamus, we show that high-frequency sensory inputs, either sinusoidal or white noise, elicit internally generated gamma (20-60 Hz) band oscillations in cortical networks. Gamma oscillations modulate cortical spike probability while preserving sub-millisecond phase relations with high-frequency sensory inputs. Consequently, our results indicate that millisecond precision stimulus-locked spiking activity and sensory-induced gamma oscillation can constitute independent multiplexed coding schemes at the single-cell level. ABSTRACT: In the natural environment, tactile exploration often leads to high-frequency vibrations at the level of the sensory organs. Single-unit recordings of cortical neurons have pointed towards either a rate or a temporal code for representing high-frequency tactile signals. In cortical networks, sensory processing results from the interaction between feedforward inputs relayed from the thalamus and internally generated activity. However, how the emergent activity represents high-frequency sensory input is not fully understood. Using multisite single-unit, local field potential and intracellular recordings in the somatosensory cortex and thalamus of lightly sedated male rats, we measured neuronal responses evoked by sinusoidal and band-pass white noise whisker stimulation at frequencies that encompass those observed during texture exploration (50-200 Hz). We found that high-frequency sensory inputs relayed from the thalamus elicit both sub-millisecond stimulus-locked responses and internally generated gamma (20-60 Hz) band oscillations in cortical networks. Gamma oscillations modulate spike probability while preserving sub-millisecond phase relations with sensory inputs. Therefore, precise stimulus-locked spiking activity and sensory-induced gamma oscillations can constitute independent multiplexed coding schemes at the single-cell level.


Asunto(s)
Potenciales de Acción , Potenciales Evocados Somatosensoriales , Neuronas/fisiología , Ruido , Corteza Somatosensorial/fisiología , Vibrisas/fisiología , Animales , Masculino , Neuronas/citología , Ratas , Ratas Sprague-Dawley , Corteza Somatosensorial/citología , Vibración
10.
J Neurosci ; 36(22): 5914-9, 2016 06 01.
Artículo en Inglés | MEDLINE | ID: mdl-27251614

RESUMEN

UNLABELLED: The role of GABAergic signaling in establishing a critical period for experience in visual cortex is well understood. However, the effects of early experience on GABAergic synapses themselves are less clear. Here, we show that monocular deprivation (MD) during the adolescent critical period produces marked enhancement of GABAergic signaling in layer 2/3 of mouse monocular visual cortex. This enhancement coincides with a weakening of glutamatergic inputs, resulting in a significant reduction in the ratio of excitation to inhibition. The potentiation of GABAergic transmission arises from both an increased number of inhibitory synapses and an enhancement of presynaptic GABA release from parvalbumin- and somatostatin-expressing interneurons. Our results suggest that augmented GABAergic inhibition contributes to the experience-dependent regulation of visual function. SIGNIFICANCE STATEMENT: Visual experience shapes the synaptic organization of cortical circuits in the mouse brain. Here, we show that monocular visual deprivation enhances GABAergic synaptic inhibition in primary visual cortex. This enhancement is mediated by an increase in both the number of postsynaptic GABAergic synapses and the probability of presynaptic GABA release. Our results suggest a contributing mechanism to altered visual responses after deprivation.


Asunto(s)
Neuronas GABAérgicas/fisiología , Inhibición Neural/fisiología , Privación Sensorial/fisiología , Sinapsis/fisiología , Corteza Visual/citología , Vías Visuales/fisiología , Factores de Edad , Animales , Animales Recién Nacidos , Channelrhodopsins , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Femenino , Lateralidad Funcional , Técnicas In Vitro , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Inhibición Neural/genética , Parvalbúminas/genética , Parvalbúminas/metabolismo , Técnicas de Placa-Clamp , Sinapsis/efectos de los fármacos , Sinapsis/genética , Potenciales Sinápticos/efectos de los fármacos , Potenciales Sinápticos/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Corteza Visual/crecimiento & desarrollo
11.
J Neurosci ; 36(25): 6778-91, 2016 06 22.
Artículo en Inglés | MEDLINE | ID: mdl-27335408

RESUMEN

UNLABELLED: Immature glutamatergic synapses in cultured neurons contain high-release probability (Pr) presynaptic sites coupled to postsynaptic sites bearing GluN2B-containing NMDA receptors (NMDARs), which mature into low-Pr, GluN2B-deficient synapses. Whether this coordinated maturation of high-Pr, GluN2B(+) synapses to low-Pr, GluN2B-deficient synapses actually occurs in vivo, and if so, what factors regulate it and what role it might play in long-term synapse function and plasticity are unknown. We report that loss of the integrin-regulated Abl2/Arg kinase in vivo yields a subpopulation of "immature" high-Pr, GluN2B(+) hippocampal synapses that are maintained throughout late postnatal development and early adulthood. These high-Pr, GluN2B(+) synapses are evident in arg(-/-) animals as early as postnatal day 21 (P21), a time that precedes any observable defects in synapse or dendritic spine number or structure in arg(-/-) mice. Using focal glutamate uncaging at individual synapses, we find only a subpopulation of arg(-/-) spines exhibits increased GluN2B-mediated responses at P21. As arg(-/-) mice age, these synapses increase in proportion, and their associated spines enlarge. These changes coincide with an overall loss of spines and synapses in the Arg-deficient mice. We also demonstrate that, although LTP and LTD are normal in P21 arg(-/-) slices, both forms of plasticity are significantly altered by P42. These data demonstrate that the integrin-regulated Arg kinase coordinates the maturation of presynaptic and postsynaptic compartments in a subset of hippocampal synapses in vivo, and this coordination is critical for NMDAR-dependent long-term synaptic stability and plasticity. SIGNIFICANCE STATEMENT: Synapses mature in vitro from high-release probability (Pr) GluN2B(+) to low-Pr, GluN2B(-), but it is unknown why this happens or whether it occurs in vivo High-Pr, GluN2B(+) synapses persist into early adulthood in Arg-deficient mice in vivo and have elevated NMDA receptor currents and increased structural plasticity. The persistence of these high-Pr, GluN2B(+) synapses is associated with a net synapse loss and significant disruption of normal synaptic plasticity by early adulthood. Together, these observations suggest that the maturation of high-Pr, GluN2B(+) synapses to predominantly low-Pr, GluN2B(-) synapses may be essential to preserving a larger dynamic range for plasticity while ensuring that connectivity is distributed among a greater number of synapses for optimal circuit function.


Asunto(s)
Espinas Dendríticas/fisiología , Regulación del Desarrollo de la Expresión Génica/genética , Hipocampo/citología , Plasticidad Neuronal/fisiología , Proteínas Tirosina Quinasas/deficiencia , Sinapsis/fisiología , Factores de Edad , Animales , Animales Recién Nacidos , Espinas Dendríticas/genética , Fármacos actuantes sobre Aminoácidos Excitadores/farmacología , Femenino , Ácido Glutámico/farmacología , Proteínas HSP70 de Choque Térmico/metabolismo , Hipocampo/crecimiento & desarrollo , Técnicas In Vitro , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Plasticidad Neuronal/efectos de los fármacos , Plasticidad Neuronal/genética , Neurotransmisores/farmacología , Proteínas Tirosina Quinasas/genética , Receptores de Glutamato/metabolismo , Receptores de N-Metil-D-Aspartato/genética , Receptores de N-Metil-D-Aspartato/metabolismo , Fracciones Subcelulares/efectos de los fármacos , Fracciones Subcelulares/metabolismo , Sinapsis/efectos de los fármacos , Potenciales Sinápticos/efectos de los fármacos , Potenciales Sinápticos/genética
12.
Cell Rep ; 43(7): 114470, 2024 Jul 23.
Artículo en Inglés | MEDLINE | ID: mdl-38985682

RESUMEN

The importance of visual cues for navigation and goal-directed behavior is well established, although the neural mechanisms supporting sensory representations in navigational circuits are largely unknown. Navigation is fundamentally dependent on the medial entorhinal cortex (MEC), which receives direct projections from neocortical visual areas, including the retrosplenial cortex (RSC). Here, we perform high-density recordings of MEC neurons in awake, head-fixed mice presented with simple visual stimuli and assess the dynamics of sensory-evoked activity. We find that a large fraction of neurons exhibit robust responses to visual input. Visually responsive cells are located primarily in layer 3 of the dorsal MEC and can be separated into subgroups based on functional and molecular properties. Furthermore, optogenetic suppression of RSC afferents within the MEC strongly reduces visual responses. Overall, our results demonstrate that the MEC can encode simple visual cues in the environment that may contribute to neural representations of location necessary for accurate navigation.


Asunto(s)
Corteza Entorrinal , Animales , Corteza Entorrinal/fisiología , Ratones , Neuronas/fisiología , Masculino , Ratones Endogámicos C57BL , Estimulación Luminosa , Optogenética , Señales (Psicología)
13.
bioRxiv ; 2024 Mar 07.
Artículo en Inglés | MEDLINE | ID: mdl-38496673

RESUMEN

GABAergic inhibition is critical to the proper development of neocortical circuits. However, GABAergic interneurons are highly diverse and the developmental roles of distinct inhibitory subpopulations remain largely unclear. Dendrite-targeting, somatostatin-expressing interneurons (SST-INs) in the mature cortex regulate synaptic integration and plasticity in excitatory pyramidal neurons (PNs) and exhibit unique feature selectivity. Relatively little is known about early postnatal SST-IN activity or impact on surrounding local circuits. We examined juvenile SST-INs and PNs in mouse primary visual cortex. PNs exhibited stable visual responses and feature selectivity from eye opening onwards. In contrast, SST-INs developed visual responses and feature selectivity during the third postnatal week in parallel with a rapid increase in excitatory synaptic innervation. SST-INs largely exerted a multiplicative effect on nearby PN visual responses at all ages, but this impact increased over time. Our results identify a developmental window for the emergence of an inhibitory circuit mechanism for normalization.

14.
Nat Neurosci ; 27(1): 148-158, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-38036743

RESUMEN

Experimental work across species has demonstrated that spontaneously generated behaviors are robustly coupled to variations in neural activity within the cerebral cortex. Functional magnetic resonance imaging data suggest that temporal correlations in cortical networks vary across distinct behavioral states, providing for the dynamic reorganization of patterned activity. However, these data generally lack the temporal resolution to establish links between cortical signals and the continuously varying fluctuations in spontaneous behavior observed in awake animals. Here, we used wide-field mesoscopic calcium imaging to monitor cortical dynamics in awake mice and developed an approach to quantify rapidly time-varying functional connectivity. We show that spontaneous behaviors are represented by fast changes in both the magnitude and correlational structure of cortical network activity. Combining mesoscopic imaging with simultaneous cellular-resolution two-photon microscopy demonstrated that correlations among neighboring neurons and between local and large-scale networks also encode behavior. Finally, the dynamic functional connectivity of mesoscale signals revealed subnetworks not predicted by traditional anatomical atlas-based parcellation of the cortex. These results provide new insights into how behavioral information is represented across the neocortex and demonstrate an analytical framework for investigating time-varying functional connectivity in neural networks.


Asunto(s)
Neocórtex , Neuronas , Ratones , Animales , Neuronas/fisiología , Imagen por Resonancia Magnética , Vigilia , Neocórtex/diagnóstico por imagen , Mapeo Encefálico/métodos , Vías Nerviosas/fisiología
15.
J Am Chem Soc ; 135(16): 5954-7, 2013 Apr 24.
Artículo en Inglés | MEDLINE | ID: mdl-23577752

RESUMEN

We have synthesized a 7-diethylaminocoumarin (DEAC) derivative that allows wavelength-selective two-photon uncaging at 900 nm versus 720 nm. This new caging chromophore, called DEAC450, has an extended π-electron moiety at the 3-position that shifts the absorption spectrum maximum of DEAC from 375 to 450 nm. Two-photon excitation at 900 nm was more than 60-fold greater than at 720 nm. Two-photon uncaging of DEAC450-Glu at 900 nm at spine heads on pyramidal neurons in acutely isolated brain slices generated postsynaptic responses that were similar to spontaneous postsynaptic excitatory miniature currents, whereas significantly higher energies at 720 nm evoked no currents. Since many nitroaromatic caged compounds are two-photon active at 720 nm, optically selective uncaging of DEAC450-caged biomolecules at 900 nm may allow facile two-color optical interrogation of bimodal signaling pathways in living tissue with high resolution for the first time.


Asunto(s)
Ácido Glutámico/química , Neuroimagen/métodos , Animales , Química Encefálica , Cumarinas/química , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Indicadores y Reactivos , Ratones , Técnicas de Placa-Clamp , Fotólisis , Células Piramidales/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Espectrofotometría Ultravioleta
16.
Eur J Neurosci ; 37(9): 1541-9, 2013 May.
Artículo en Inglés | MEDLINE | ID: mdl-23387435

RESUMEN

The neuropeptide galanin has been shown to alter the rewarding properties of morphine. To identify potential cellular mechanisms that might be involved in the ability of galanin to modulate opiate reward, we measured excitatory postsynaptic potentials (EPSPs), using both field and whole-cell recordings from striatal brain slices extracted from wild-type mice and mice lacking specific galanin receptor (GalR) subtypes. We found that galanin decreased the amplitude of EPSPs in both the dorsal striatum and nucleus accumbens. We then performed recordings in slices from knockout mice lacking either the GalR1 or GalR2 gene, and found that the ability of galanin to decrease EPSP amplitude was absent from both mouse lines, suggesting that both receptor subtypes are required for this effect. In order to determine whether behavioral responses to opiates were dependent on the same receptor subtypes, we tested GalR1 and GalR2 knockout mice for morphine conditioned place preference (CPP). Morphine CPP was significantly attenuated in both GalR1 and GalR2 knockout mice. These data suggest that mesolimbic excitatory signaling is significantly modulated by galanin in a GalR1-dependent and GalR2-dependent manner, and that morphine CPP is dependent on the same receptor subtypes.


Asunto(s)
Condicionamiento Clásico , Cuerpo Estriado/fisiología , Potenciales Postsinápticos Excitadores , Núcleo Accumbens/fisiología , Receptor de Galanina Tipo 1/metabolismo , Receptor de Galanina Tipo 2/metabolismo , Animales , Cuerpo Estriado/efectos de los fármacos , Cuerpo Estriado/metabolismo , Galanina/farmacología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Morfina/farmacología , Núcleo Accumbens/efectos de los fármacos , Núcleo Accumbens/metabolismo , Receptor de Galanina Tipo 1/genética , Receptor de Galanina Tipo 2/genética
17.
bioRxiv ; 2023 Oct 04.
Artículo en Inglés | MEDLINE | ID: mdl-37873152

RESUMEN

The ability of rodents to use visual cues for successful navigation and goal-directed behavior has been long appreciated, although the neural mechanisms supporting sensory representations in navigational circuits are largely unknown. Navigation is fundamentally dependent on the hippocampus and closely connected entorhinal cortex, whose neurons exhibit characteristic firing patterns corresponding to the animal's location. The medial entorhinal cortex (MEC) receives direct projections from sensory areas in the neocortex, suggesting the ability to encode sensory information. To examine this possibility, we performed high-density recordings of MEC neurons in awake, head-fixed mice presented with simple visual stimuli and assessed the dynamics of sensory-evoked activity. We found a large fraction of neurons exhibited robust responses to visual input that shaped activity relative to ongoing network dynamics. Visually responsive cells could be separated into subgroups based on functional and molecular properties within deep layers of the dorsal MEC, suggesting diverse populations within the MEC contribute to sensory encoding. We then showed that optogenetic suppression of retrosplenial cortex afferents within the MEC strongly reduced visual responses. Overall, our results demonstrate the the MEC can encode simple visual cues in the environment that can contribute to neural representations of location necessary for accurate navigation.

18.
Epilepsia ; 53(8): 1429-35, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22612065

RESUMEN

PURPOSE: Most common forms of human epilepsy result from a complex combination of polygenetic and environmental factors. Quantitative trait locus (QTL) mapping is a first step toward the nonbiased discovery of epilepsy-related candidate genes. QTL studies of susceptibility to induced seizures in mouse strains have consistently converged on a distal region of chromosome 1 as a major phenotypic determinant; however, its influence on spontaneous epilepsy remains unclear. In the present study we characterized the influence of allelic variations within this QTL, termed Szs1, on the occurrence of spontaneous spike-wave discharges (SWDs) characteristic of absence seizures in DBA/2 (D2) mice. METHODS: We analyzed SWD occurrence and patterns in freely behaving D2, C57BL/6 (B6) and the congenic strains D2.B6-Szs1 and B6.D2-Szs1. KEY FINDINGS: We showed that congenic manipulation of the Szs1 locus drastically reduced the number and the duration of SWDs in D2.B6-Szs1 mice, which are homozygous for Szs1 from B6 strain on a D2 strain background. However, it failed to induce the full expression of SWDs in the reverse congenic animals B6.D2-Szs1. SIGNIFICANCE: Our results demonstrate that the occurrence of SWDs in D2 animals is under polygenic control and, therefore, the D2 and B6 strains might be a useful model to dissect the genetic determinants of polygenic SWDs characteristic of typical absence seizures. Furthermore, we point to the existence of epistatic interactions between at least one modifier gene within Szs1 and genes within unlinked QTLs in regulating the occurrence of spontaneous nonconvulsive forms of epilepsies.


Asunto(s)
Mapeo Cromosómico , Epilepsia Tipo Ausencia/genética , Sitios de Carácter Cuantitativo/genética , Animales , Animales Congénicos/genética , Encéfalo/fisiopatología , Electroencefalografía , Epilepsia Tipo Ausencia/fisiopatología , Predisposición Genética a la Enfermedad/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos DBA
19.
Neurophotonics ; 9(3): 032202, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-36159712

RESUMEN

Brain organization is evident across spatiotemporal scales as well as from structural and functional data. Yet, translating from micro- to macroscale (vice versa) as well as between different measures is difficult. Reconciling disparate observations from different modes is challenging because each specializes within a restricted spatiotemporal milieu, usually has bounded organ coverage, and has access to different contrasts. True intersubject biological heterogeneity, variation in experiment implementation (e.g., use of anesthesia), and true moment-to-moment variations in brain activity (maybe attributable to different brain states) also contribute to variability between studies. Ultimately, for a deeper and more actionable understanding of brain organization, an ability to translate across scales, measures, and species is needed. Simultaneous multimodal methods can contribute to bettering this understanding. We consider four modes, three optically based: multiphoton imaging, single-photon (wide-field) imaging, and fiber photometry, as well as magnetic resonance imaging. We discuss each mode as well as their pairwise combinations with regard to the definition and study of brain networks.

20.
Curr Opin Neurobiol ; 77: 102627, 2022 12.
Artículo en Inglés | MEDLINE | ID: mdl-36115252

RESUMEN

Investigating links between nervous system function and behavior requires monitoring neuronal activity at a range of spatial and temporal scales. Here, we summarize recent progress in applying two distinct but complementary approaches to the study of network dynamics in the neocortex. Mesoscopic calcium imaging allows simultaneous monitoring of activity across most of the cortex at moderate spatiotemporal resolution. Electrophysiological recordings provide extremely high temporal resolution of neural signals at multiple targeted locations. A number of recent studies have used these tools to reveal novel patterns of activity across distributed cortical subnetworks. This growing body of work strongly supports the hypothesis that the dynamic coordination of spatially distinct regions is a fundamental aspect of cortical function that supports cognition and behavior.


Asunto(s)
Neocórtex , Neocórtex/fisiología , Neuronas/fisiología , Cognición , Fenómenos Electrofisiológicos , Calcio
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