Validation of cell-based fluorescence assays: practice guidelines from the ICSH and ICCS - part III - analytical issues.

Clinical diagnostic assays, may be classified as quantitative, quasi-quantitative or qualitative. The assay's description should state what the assay needs to accomplish (intended use or purpose) and what it is not intended to achieve. The type(s) of samples (whole blood, peripheral blood mononuclear cells (PBMC), bone marrow, bone marrow mononuclear cells (BMMC), tissue, fine needle aspirate, fluid, etc.), instrument platform for use and anticoagulant restrictions should be fully validated for stability requirements and specified. When applicable, assay sensitivity and specificity should be fully validated and reported; these performance criteria will dictate the number and complexity of specimen samples required for validation. Assay processing and staining conditions (lyse/wash/fix/perm, stain pre or post, time and temperature, sample stability, etc.) should be described in detail and fully validated.

Use of temporal and spatial information in estimating event completion time.

In this research, we investigated how students use temporal and spatial information to estimate the time to fill a tank. In Experiment 1, we evaluated the usefulness of a digital clock to calibrate temporal information and of an overflow tank to calibrate spatial information and investigated how component skills, such as judging volume, judging time, and performing mental calculations, correlate with estimation accuracy. In Experiment 2, we compared the usefulness of static and animated displays in order to test the prediction that static displays would be more useful when the fill rate remained constant but animated displays would be more useful when the fill rate changed. The findings, when combined with verbal reports, provide evidence regarding the use of four strategies that differ in their perceptual and arithmetic demands.