RESUMEN
Nitrogen availability in the rhizosphere relies on root-microorganism interactions, where root exudates trigger soil organic matter (SOM) decomposition through the rhizosphere priming effect (RPE). Though microbial necromass contribute significantly to organically bound soil nitrogen (N), the role of RPEs in regulating necromass recycling and plant nitrogen acquisition has received limited attention. We used 15N natural abundance as a proxy for necromass-N since necromass is enriched in 15N compared to other soil-N forms. We combined studies using the same experimental design for continuous 13CO2 labelling of various plant species and the same soil type, but considering top- and subsoil. RPE were quantified as difference in SOM-decomposition between planted and unplanted soils. Results showed higher plant N uptake as RPEs increased. The positive relationship between 15N-enrichment of shoots and roots and RPEs indicated an enhanced necromass-N turnover by RPE. Moreover, our data revealed that RPEs were saturated with increasing carbon (C) input via rhizodeposition in topsoil. In subsoil, RPEs increased linearly within a small range of C input indicating a strong effect of root-released C on decomposition rates in deeper soil horizons. Overall, this study confirmed the functional importance of rhizosphere C input for plant N acquisition through enhanced necromass turnover by RPEs.
Asunto(s)
Nitrógeno , Raíces de Plantas , Rizosfera , Microbiología del Suelo , Carbono/metabolismo , Nitrógeno/metabolismo , Isótopos de Nitrógeno , Raíces de Plantas/microbiología , Raíces de Plantas/metabolismo , Brotes de la Planta/metabolismo , Brotes de la Planta/microbiología , Plantas/metabolismo , Plantas/microbiología , Suelo/químicaRESUMEN
Consequences of interactions between ectomycorrhizal fungi (EcMF) and non-mycorrhizal rhizosphere fungi (NMRF) for plant carbon (C) allocation belowground and nutrient cycling in soil remain unknown. To address this topic, we performed a mesocosm study with Norway spruce seedlings [Picea abies (L.) H. Karst] inoculated with EcMF, NMRF, or a mixture of both (MIX). 14 CO2 pulse labelling of spruce was applied to trace and visualize the 14 C incorporation into roots, rhizohyphosphere and hyphosphere. Activities and localization of enzymes involved in the C, nitrogen (N) and phosphorus (P) cycling were visualized using zymography. Spruce seedlings inoculated with EcMF and NMRF allocated more C to soils (EcMF: 10.7%; NMRF: 3.5% of total recovered C) compared to uninoculated control seedlings. The 14 C activity in the hyphosphere was highest for EcMF and lowest for NMRF. In the presence of both, NMRF and EcMF (MIX), the 14 C activity was 64% lower compared with EcMF inoculation alone. This suggests a suppressed C allocation via EcMF likely due to the competition between EcMF and NMRF for N and P. Furthermore, we observed 57% and 49% higher chitinase and leucine-aminopeptidase activities in the rhizohyphosphere of EcMF compared to the uninoculated control, respectively. In contrast, ß-glucosidase activity (14.3 nmol cm-2 h-1 ) was highest in NMRF likely because NMRF consumed rhizodeposits efficiently. This was further supported by that enzyme stoichiometry in soil with EcMF shifted to a higher investment of nutrient acquisition enzymes (e.g., chitinase, leucine-aminopeptidase, acid phosphatase) compared to NMRF inoculation, where investment in ß-glucosidase increased. In conclusion, the alleviation of EcMF from C limitation promotes higher activities of enzymes involved in the N and P cycle to cover the nutrient demand of EcMF and host seedlings. In contrast, C limitation of NMRF probably led to a shift in investment towards higher activities of enzymes involved in the C cycle.
Asunto(s)
Abies , Celulasas , Quitinasas , Micorrizas , Picea , Pinus , Aminopeptidasas/metabolismo , Quitinasas/metabolismo , Hongos , Leucina/metabolismo , Micorrizas/metabolismo , Picea/metabolismo , Raíces de Plantas/microbiología , Rizosfera , Plantones/metabolismo , SueloRESUMEN
Background and Aims: Although it is commonly accepted that root exudation enhances plant-microbial interactions in the rhizosphere, experimental data on the spatial distribution of exudates are scarce. Our hypothesis was that root hairs exude organic substances to enlarge the rhizosphere farther from the root surface. Methods: Barley (Hordeum vulgare 'Pallas' - wild type) and its root-hairless mutant (brb) were grown in rhizoboxes and labelled with 14CO2. A filter paper was placed on the soil surface to capture, image and quantify root exudates. Key Results: Plants with root hairs allocated more carbon (C) to roots (wild type: 13 %; brb: 8 % of assimilated 14C) and to rhizosheaths (wild type: 1.2 %; brb: 0.2 %), while hairless plants allocated more C to shoots (wild type: 65 %; brb: 75 %). Root hairs increased the radial rhizosphere extension three-fold, from 0.5 to 1.5 mm. Total exudation on filter paper was three times greater for wild type plants compared to the hairless mutant. Conclusion: Root hairs increase exudation and spatial rhizosphere extension, which probably enhance rhizosphere interactions and nutrient cycling in larger soil volumes. Root hairs may therefore be beneficial to plants under nutrient-limiting conditions. The greater C allocation below ground in the presence of root hairs may additionally foster C sequestration.
Asunto(s)
Carbono/metabolismo , Raíces de Plantas/metabolismo , Rizosfera , Hordeum/metabolismo , Suelo/químicaRESUMEN
Modern agriculture's goal of improving crop resource acquisition efficiency relies on the intricate relationship between the root system and the soil. Root and rhizosphere traits play a critical role in the efficient use of nutrients and water, especially under dynamic environments. This review emphasizes a holistic perspective, challenging the conventional separation of nutrient and water uptake processes and the necessity for an integrated approach. Anticipating climate change-induced increase in the likelihood of extreme weather events that result in fluctuations in soil moisture and nutrient availability, the study explores the adaptive potential of root and rhizosphere traits to mitigate stress. We emphasize the significance of root and rhizosphere characteristics that enable crops to rapidly respond to varying resource availabilities (i.e. the presence of water and mobile nutrients in the root zone) and their accessibility (i.e. the possibility to transport resources to the root surface). These traits encompass for example root hairs, mucilage and extracellular polymeric substance (EPS) exudation, rhizosheath formation and the expression of nutrient and water transporters. Moreover, we recognize the challenge of balancing carbon investments, especially under stress, where optimized traits must consider carbon-efficient strategies. To advance our understanding, the review calls for well-designed field experiments, recognizing the limitations of controlled environments. Non-destructive methods such as mini rhizotron assessments and in-situ stable isotope techniques, in combination with destructive approaches such as root exudation analysis, are proposed for assessing root and rhizosphere traits. The integration of modeling, experimentation, and plant breeding is essential for developing resilient crop genotypes capable of adapting to evolving resource limitation.
RESUMEN
Erosion-induced topsoil dilution strongly affects cropland biogeochemistry and is associated with a negative effect on soil health and crop productivity. While its impact on soil C cycling has been widely recognized, there is little information about its impact on soil N cycling and N fertilizer dynamics. Here, we studied three factors potentially influencing N cycling and N fertilizer dynamics in cropping systems, namely: 1.) soil type, 2.) erosion-induced topsoil dilution and 3.) N fertilizer form, in a full-factorial pot experiment using canola plants. We studied three erosion affected soil types (Luvisol, eroded Luvisol, calcaric Regosol) and performed topsoil dilution in all three soils by admixing 20 % of the respective subsoil into its topsoil. N fertilizer dynamics were investigated using either mineral (calcium ammonium nitrate) or organic (biogas digestate) fertilizer, labeled with 15N. The fertilizer 15N recovery and the distribution of the fertilizer N in different soil fractions was quantified after plant maturity. Fertilizer N dynamics and utilization were influenced by all three factors investigated. 15N recovery in the plant-soil system was higher and fertilizer N utilization was lower in the treatments with diluted topsoil than in the non-diluted controls. Similarly, plants of the organic fertilizer N treatments took up significantly less fertilizer N in comparison to mineral fertilizer treatments. Both topsoil dilution and organic fertilizer application promoted 15N recovery and N accumulation in the soil fractions, with strong differences between soil types. Our study reveals an innovative insight: topsoil dilution due to soil erosion has a negligible impact on N cycling and dynamics in the plant-soil system. The crucial factors influencing these processes are found to be the choice of fertilizer form and the specific soil type. Recognizing these aspects is essential for a precise and comprehensive assessment of the environmental continuum, emphasizing the novelty of our findings.
RESUMEN
Phosphorus (P) availability severely limits plant growth due to its immobility and inaccessibility in soils. Yet, visualization and measurements of P uptake from different root types or regions in soil are methodologically challenging. Here, we explored the potential of phosphor imaging combined with local injection of radioactive 33P to quantitatively visualize P uptake and translocation along roots of maize grown in soils. Rhizoboxes (20 × 40 × 1 cm) were filled with sandy field soil or quartz sand, with one maize plant per box. Soil compartments were created using a gravel layer to restrict P transfer. After 2 weeks, a compartment with the tip region of a seminal root was labeled with a NaH2 33PO4 solution containing 12 MBq of 33P. Phosphor imaging captured root P distribution at 45 min, 90 min, 135 min, 180 min, and 24 h post-labeling. After harvest, 33P levels in roots and shoots were quantified. 33P uptake exhibited a 50% increase in quartz sand compared to sandy soil, likely attributed to higher P adsorption to the sandy soil matrix than to quartz sand. Notably, only 60% of the absorbed 33P was translocated to the shoot, with the remaining 40% directed to growing root tips of lateral or seminal roots. Phosphor imaging unveiled a continuous rise in 33P signal in the labeled seminal root from immediate post-labeling until 24 h after labeling. The highest 33P activities were concentrated just above the labeled compartment, diminishing in locations farther away. Emerging laterals from the labeled root served as strong sinks for 33P, while a portion was also transported to other seminal roots. Our study quantitatively visualized 33P uptake and translocation dynamics, facilitating future investigations into diverse root regions/types and varying plant growth conditions. This improves our understanding of the significance of different P sources for plant nutrition and potentially enhances models of plant P uptake.
RESUMEN
As plant roots take up water and the soil dries, water depletion is expected to occur in the rhizosphere. However, recent experiments showed that the rhizosphere was wetter than the bulk soil during root water uptake. We hypothesise that the increased water content in the rhizosphere was caused by mucilage exuded by roots. It is probably that the higher water content in the rhizosphere results in higher hydraulic conductivity of the root-soil interface. In this case, mucilage exudation would favour the uptake of water in dry soils. To test this hypothesis, we covered a suction cup, referred to as an artificial root, with mucilage. We placed it in soil with a water content of 0.03cm3cm-3, and used the root pressure probe technique to measure the hydraulic conductivity of the root-soil continuum. The results were compared with measurements with roots not covered with mucilage. The root pressure relaxation curves were fitted with a model of root water uptake including rhizosphere dynamics. The results demonstrated that when mucilage is added to the root surface, it keeps the soil near the roots wet and hydraulically well conductive, facilitating the water flow from dry soils towards the root surface. Mucilage exudation seems to be an optimal plant trait that favours the capture of water when water is scarce.