Dried fish more prone to microplastics contamination over fresh fish - Higher potential of trophic transfer to human body.

Globally, microplastics (MPs) contamination in aquatic organisms is emerging as an alarming phenomenon. In the present study, we investigated MPs in three commercially important fishes (Bombay duck Harpadon nehereus, ribbon fish Trichiurus lepturus and hairfin anchovy Setipinna phasa) in fresh and dried conditions collected from two sites (Chattogram and Kuakata) of the Bay of Bengal. It was evident that fresh T. lepturus ingested highest amount of MPs through the gills (6.41 mps/g) from Chattogram followed by in the gastrointestinal tract, GIT (6.20 mps/g) and in the muscle (1.20 mps/g) from Kuakata. Among the fresh fishes, H. nehereus from Kuakata accumulated highest amount of MPs (0.21 mps/g), while S. phasa from Kuakata contained the least amount of MPs (0.06 mps/g). On the other hand, among the dried fishes, T. lepturus from Kuakata contained highest amount of MPs (46.00 mps/g), while S. phasa from Kuakata retained lowest amount of MPs (2.17 mps/g). Strangely, all the dried fishes showed significantly higher amount of MPs compared to fresh fishes from both the locations. Fiber was the most dominant type of shape of MPs which accounted 66 %, followed by fragment (27.38 %), microbeads (3.59 %), film (1.48 %), foam (1.31 %) and pellet (0.25 %). Size-wise, the major portion (39.66 %) of MPs was present to be in size range less than 0.5 mm followed by 37.67 % in the size range of 0.5-1.0 mm group and rest 22.67 % within 1.0-5.0 mm. Red (41.55 %) colored MPs was the most prominent, followed by brown (22.11 %), blue (16.32 %), pink (11.69 %), purple (5.10 %), and green (2.25 %). Among polymer types, low-density polyethylene (LDPE) was the most common (38 %), followed by polystyrene (PS-22 %), polyvinyl chloride (PVC-16 %), polyamide (PA-13 %) and ethylene-vinyl acetate (EVA-9 %). The present study confirms high occurrence of MPs in the dried fishes over the fresh fishes from the Bay of Bengal, with high potential of trophic transfer to the human body.

Knowledge, practice, and economic impacts of COVID-19 on small-scale coastal fishing communities in Bangladesh: Policy recommendations for improved livelihoods.

COVID-19 has profoundly impacted global livelihoods and disrupted the food supply chain, including the aquaculture and fisheries industries. Little is known about the response to COVID-19 and the impact it has on incomes, livelihoods and knowledge and practice in the coastal artisanal fishers communities of Bangladesh. Therefore, the aim of this study was to determine the socio-demographics of selected coastal fishers, their knowledge about COVID-19 and the preventive practice taken to reduce it. The impact on their fishing habits and income was also examined to determine potential policy areas. Data were collected via a structured questionnaire from 250 respondents from three coastal districts, Cox's Bazar, Patuakhali and Barguna, Bangladesh during April-June 2020. The research shows that the fishers' knowledge about COVID-19 and measures taken to reduce it were significantly higher in Patuakhali and Barguna than in Cox's Bazar. The pandemic caused lower consumer demand, reduced fish prices and created fish transportation issues due to movement restrictions enforced during the lockdown. Irrespective of geographical location, fishing trips were reduced by frequency and duration compared with the pre-COVID-19 period, consequently lowering the income of fishers. Fishers have received little or no support from private, non-governmental or governmental sources. Considering the evidence in this paper of economic hardship, this paper recommends artisanal fishers in Bangladesh should be provided with support to improve their health education, access to professional health facilities and financial services. This will contribute to improved food security and sustainable livelihoods that can better withstand local and/or global crises.

Evaluation of occupational health management status and safety issues of the small-scale fisheries sector in Bangladesh.

BACKGROUND: Small-scale fishing is one of the most precarious occupations, with high rates of threats and hazards. The present study was undertaken to evaluate the health hazards and safety issues of fishers involved in small-scale fisheries (SSF). MATERIALS AND METHODS: Fifty SSF fishers (n = 50) were surveyed by using a pre-tested questionnaire between October 2019 and March 2020 at the lower Meghna River in the northern tip of the Bay of Bengal, Bangladesh. RESULTS: Results revealed that 56% of SSF fishermen belong to a nuclear family, and 42% completed primary education. Forty per cent had an annual income of between 1,000 and 1,500 USD. Seventy-six per cent of fishermen were found to suffer from fever, and 72%, and 60% from diarrhoea and skin diseases over the last 5 years (2015-2020), respectively. During fishing, 78% of fishermen also suffered from red-eye problems, dizziness, and headache, and 68% struggled with musculoskeletal complaints during the last 5 years. Extreme cyclonic occurrences and sudden storms were experienced by 66% and 32% of fishermen, respectively, during the last 5 years. Local pharmacies were visited by 46% of fishermen for treatment due to ease of access. Sixty-four per cent of participants applied their local indigenous knowledge to treat health-related problems. Twenty-eight per cent and 32% of fishermen used a first aid box and stored medicine on board, respectively. CONCLUSIONS: Most of the fishers are in great risk of medium- to high-range danger while fishing in the SSF sector in Bangladesh. Many countries have developed protocols for safe and responsible fishing. In Bangladesh, adequate attention is needed for the sustainable development of the SSF sector.

Nutritional, microbial and various quality aspects of common dried fish from commercial fish drying centers in Bangladesh.

The purpose of this study was to evaluate the nutritional, microbial and sensory quality of five dried fish species from five fish drying centers in Bangladesh, and consumers' perception on quality and perceived health problems of consuming dried fish. Proximate composition and bacterial load were determined following AOAC and total plate count method, respectively. Data on peoples' perception regarding the quality aspects of dried fish were collected using a structured questionnaire. Sensory analysis showed samples from Cox's Bazar had higher acceptability than other regions. Moisture content ranged from 12.00 ± 1.12% to 22.99 ± 1.09%, the highest found in Bombay duck from Patuakhali. The highest values of protein were found in shrimp from Chittagong (64.33 ± 0.99%) and the lowest in Bombay duck from Bhola (51.80 ± 0.95%). The lipid content varied from 5.38 ± 0.37% (Bombay duck from Cox's Bazar) to 8.67 ± 0.96% (Bombay duck from Khulna). The ash content was ranged from 13.89 ± 0.94% to 20.07 ± 1.64% in Bombay duck from Patuakhali and Cox's Bazar. The mean total plate count of dried Bombay duck were 7.1 ± 0.2×107, 9.8 ± 0.1×107 and 7.8 ± 0.52 × 107 cfu/g, whereas, total Vibrio spp. count were estimated 1.1 ± 0.1×103, 3.7 ± 0.2×105 and 1.8 ± 0.1 × 105 cfu/g for Chittagong, Cox's Bazar and Bhola, respectively. The pathogenic bacterial species E. coli and Salmonella sp. were absent in dried Bombay duck from all locations. Of 500 respondents, the majority (94.8%) reported no complications after consuming dried fish. Significant quality variation among the dried fish samples suggested further improvement in dried fish quality through maintaining hygiene and sanitation to produce quality and safe dried fish for the consumers in home and abroad.

Probabilistic public health risks associated with pesticides and heavy metal exposure through consumption of common dried fish in coastal regions of Bangladesh.

The chemical contaminants in dried fish are of great food safety concern and an emerging public health issue in Bangladesh. The aim of this study was to assess the public health risk associated with exposure to pesticides (organochlorine and organophosphorus) and heavy metals (lead, mercury, cadmium, chromium, arsenic) through the consumption of dried fish (Bombay duck, ribbon fish, silver jewfish, shrimp, Chinese promfret) in coastal districts (Cox's Bazar, Chittagong, Bhola, Patuakhali, Khulna) of Bangladesh. Dried fish consumption data were collected from 500 adult respondents (100 from each district) using a food frequency questionnaire (FFQ). Pesticide residues were determined using QuEChERS extraction coupled to gas chromatography and gas chromatography mass spectrometry, and heavy metals were estimated using an atomic absorption spectrophotometric method. The results revealed that the frequency and amount of dried fish consumption was highest for Bombay duck in Cox's Bazar (11.57 g/capita/day) and ribbon fish (12.10 g/capita/day) in Chittagong. The estimated daily intake (EDI, 7.40 × 10-5 to 1.10 × 10-4 mg/kg/day) and health risk index (HRI, 0.013 to 0.16) values expressed no health risk from pesticide residues in all the positive samples. For heavy metals, target hazard quotients (THQ) for non-carcinogenic health risk were below 1 (0.001-0.154), indicating no health risk for all samples. However, carcinogenic risk R value indicated a potential health risk for chromium (2.64 × 10-6 to 4.06 × 10-6), and carcinogenic RT value (2.82 × 10-6 to 5.71 × 10-6) indicated a potential health risk for all the metals. It is concluded that the risk of exposure to pesticides through the consumption of dried fish is low, while heavy metals pose moderate-to-high health risks to dried fish consumers in the study area. Thus, the study suggests an appropriate risk management policy that controls pesticides and heavy metals in dried fish to ensure safe food for local and global consumers, underpinned by a producers' capacity building and consumers' awareness raising strategy.

Biochemical properties and shelf life of value-added fish cube and powder developed from hilsa shad (Tenualosa ilisha).

Hilsa shad (Tenualosa ilisha) is one of the most popular and tastiest fishes in Southeast Asia because of its unique soft texture, mouth-watering flavor, high nutritive value with high protein, and very high lipid, attractive body shape, and highly appealing shiny appearance. Recently increased productions of hilsa, in association with high demand but very high price, make it to be a good candidate for value-addition. The major challenge of hilsa value addition lies in its high lipid content, while lipids are the reasons for its unique tastiness. Under a national initiative, the present study therefore developed novel methods to prepare hilsa cube and hilsa powder by stabilizing lipids and proteins, which contained the original taste and flavor of hilsa, and had good storability in freezing and room temperature conditions for 6 months. The pre-spawning, moderate size (600-700g) female hilsa was used for product formulation. Proximate composition, biochemical qualities (TVB-N, TMA-N, pH, PV, TBA value, histamine content) of the hilsa cube and powder were analyzed following standard methods and sensory properties were analyzed using a 5-point hedonic scale. The biochemical parameters and sensory properties of the products varied based on ingredients used and the final product states. Both the products were in good quality for 6 months of storage, since cryoprotectants protected the protein quality both during freezing and high-temperature processing. Carotenoid extract from fresh carrots was found to be effective in reducing lipid oxidation in hilsa. Biochemical attributes in all products gradually decreased with storage time (p < 0.05). Almost similar sensory attributes were observed in both hilsa powder and hilsa cube, which were gradually decreased (p > 0.05) with the progress of the storage period. Both the products were within the acceptable quality limit during all storage periods and conditions. The results suggest that hilsa cube and powder have excellent quality standards, storage stability, and the possibility for fortifying ready-to-eat value-added hilsa products for the consumers at home and aboard.

Effects of Pangasius (Pangasius hypophthalmus) and Skipjack Tuna (Sarda orientalis) mince blend on the quality of fish products: Ways to utilize resources and nutrition in Bangladesh.

This study was conducted with the aims of utilizing the resources and analyzing the quality of value-added fish products, fish ball made from a fish mince blend of pangasius (Pangasius hypophthalmus) and skipjack tuna (Sarda orientalis) (ratios P100:T0, P75:T25, P50:T50, P25:T75, P0:T100), under two cooking processes (two-step heating and autoclaving). The textural quality (softness/firmness [S/F]; chewiness/rubberiness [C/R]; and folding test [FT]) and the nutritional quality of the fish products were determined by the sensory method and AOAC method, respectively. The results showed that tuna had higher utilization than pangasius. The products from the washed fish mince blend showed better textural properties with two-step heating (50℃ for 60 min and 100℃ for 30 min) than with autoclaving (120℃, 15Ib/Inc2 for 15 min) and unwashed process. Of the five fish mince blend ratios, P50:T50 showed significant higher textural properties (FT, S/F, and C/R values) than the other ratios (p < .05). Further improvements in textural qualities were observed when the mince blend was washed with different salt solutions (0.1% NaCl, KCl, CaCl2, and MgCl2). Whitish or bright color attributes were obtained from pangasius mince, which became darker proportionately with increasing proportions of tuna mince (p < .05). The texture, color, and nutritional quality of the fish products were affected by washing, heating, and the compositional differences in the fish species. Thus, value-added fish products based on a fish mince blend could contribute to an increase in total resource utilization and nutritional security in Bangladesh.

Comparative quality assessment of traditional vs. improved dried Bombay duck (Harpodon nehereus) under different storage conditions: Solar chimney dryer a low-cost improved approach for nutritional dried fish.

Dried fish and fishery products provide important and affordable dietary sources of animal protein. The present study compared the sensory, microbial, and biochemical quality of bombay duck (Harpadon nehereous) dried fish produced using improved versus traditional open-air drying method. The sensory, microbial, and biochemical quality was determined following quality index, total plate count (TPC), and Association of Official Analytical Chemists (AOAC) method, respectively. The sensory quality results indicate highly acceptable dried fish with the improved method compared to traditional method at both initial and storage condition. The microbial load (APC, TEC, TViC, and TSC) of improved dried fish was within internationally permissible microbiological standards for food whereas traditionally dried fish exceeded the permissible limit. The biochemical composition (protein, lipid, ash, and moisture) of improved dried fish had better nutritional value than the traditional dried fish. After 60 days storage time, samples packaged under HDPE conditions exhibited excellent organoleptic characteristics, lower microbial load, and lower biochemical decomposition than samples packaged in LDPE. The above results confirmed that the solar chimney dryer produced superior quality of dried fish compared to the traditional open-air drying method both initially and after storage, while packaging dried fish under HDPE conditions extended shelf life further.

Monitoring of pesticide residues from fish feed, fish and vegetables in Bangladesh by GC-MS using the QuEChERS method.

The use of pesticides in agricultural sectors is rising due to the growing demand for food in the world, but the presence of pesticide residues in agricultural commodities has become a major health concern for consumers and is associated with problems of food safety. Thus, the present study determined pesticide residues (17 organochlorine, 5 pyrethroid and 3 organophosphate) in 77 fish feed, 112 fish and 135 vegetables samples (total of 324) from the different locations in Bangladesh, using quick easy cheap effective rugged and safe (QuEChERS) extraction followed by gas chromatography-mass spectrometry (GC-MS) analysis. The 77 fish feed samples analyzed with this method appeared to be free of pyrethroid pesticide residues. Organochlorine pesticide residues aldrin (0.03 ± 0.01 mg/kg) and pyrethroid pesticide residues permethrin (0.08 ± 0.01 mg/kg) were detected in fish samples of catla (Catla catla) from Rajshahi Durgapur and bata (Labeo bata) from Satkhira Kaligonj, respectively. Among 135 vegetable samples (country bean, green chili, tomato, eggplants and red amaranth), 27.4% were found positive for organophosphate pesticide residues of which 89.2% exceeded maximum residue limits (MRLs) set by the Codex Alimentarius Commission. The study revealed that few fish (catla and bata) but the majority of vegetable samples tested positive for pesticide residues exceeding MRLs. Finally, the study suggests that an effective management strategy is needed for strict regulation and regular monitoring of pesticides in fish feed, fish and vegetables to make aware the farmers and consumers about the harmful effect of pesticides on human health.

Prospects and challenges of yellow flesh pangasius in international markets: secondary and primary evidence from Bangladesh.

The projected increase in aquaculture production by 2030 will mostly occur in countries of Asia and Africa, including Bangladesh. The pangasius (Pangasianodon hypophthalmus) produced in Bangladesh, the second-largest producer globally, is mainly consumed by low-income domestic consumers and is poorly demanded in international markets. One reason for this is the yellow flesh of fish; consumers generally in mainstream international markets prefer to fish with white flesh. Reviewing secondary evidence and analyzing primary data, this article assesses the underlying reasons for the discolored pangasius flesh in Bangladesh and synthesizes strategies for avoiding discoloration to induce exports. The findings indicate that farming practices with high stocking density, infrequent water exchange, high organic matter in pond water, and the growth of carotenoid-containing cyanobacteria contribute to the discoloration of pangasius flesh. Artificial and natural pigments in feed and poor post-harvest handling of fish are also contributing factors. Furthermore, a positive correlation between water exchange, price, and yield at the farm is found, which indicates that farm-gate price and yield per hectare can increase with more frequent water exchange. The findings of this study provide strong evidence that improved aquaculture practices can solve the problem of discolored pangasius flesh and establish an export-oriented pangasius industry in Bangladesh.

Evaluation of artificially contaminated fish with formaldehyde under laboratory conditions and exposure assessment in freshwater fish in Southern Bangladesh.

Formalin can be added as preservative to fresh foods to prevent spoilage and extend shelf life. Formalin contains 37-40% formaldehyde, which is classified as carcinogenic to humans. To assess the public health risk associated with formaldehyde exposure in freshwater fish in Southern Bangladesh, formaldehyde concentrations (mg/kg) were determined in tilapia, Indian major carp rui, Chinese carp and a minor carp from local market and in laboratory simulations (0.5, 1.0, 2.0 and 4.0% formaldehyde solution for 5, 15, 30 and 60 min) with spectrophotometric and high performance liquid chromatography (HPLC) methods. A food frequency questionnaire was used to collect fish consumption (kg/kg BW. d) data from 400 respondents. A probabilistic exposure assessment was conducted using @Risk®7.0 software. Fish treated with formalin at increasing concentrations and exposure time showed increased trends of formaldehyde acquisition irrespective of fish species and analytical methods used (p < .05). Compared to spectrophotometry, the HPLC method was shown to be more sensitive and is therefore the preferred method for formalin quantification. Maximum exposure to formaldehyde (0.28 mg/kg BW. d) was calculated for tilapia using HPLC analysis. Margin of exposure (MoE) provides high priority (<10,000) for tilapia and Indian major carp rui at P99 under spectrophotometric analysis whereas as determined using HPLC, tilapia had MoE values much lower than 10,000 at P99, P95 and P90 (both total population and consumers). Exposure to formaldehyde associated with freshwater fish consumption is a public health concern in Southern Bangladesh and needs further assessment and risk management strategies.

Properties of blend film based on cuttlefish (Sepia pharaonis) skin gelatin and mungbean protein isolate.

Blend films based on cuttlefish (Sepia pharaonis) ventral skin gelatin (CG) and mungbean protein isolate (MPI) at different blend ratios (CG/MPI=10:0, 8:2, 6:4, 4:6, 2:8 and 0:10, w/w) prepared at pH 11 using 50% glycerol (based on total protein) as plasticizer were characterized. CG films incorporated with MPI at increasing amounts had the decreases in tensile strength (TS) (p<0.05). The increases in elongation at break (EAB) were observed when CG/MPI ratios of 6:4 or 4:6 were used (p<0.05). Decreased water vapor permeability (WVP) was obtained for films having the increasing proportion of MPI (p<0.05). CG/MPI blend films with higher MPI proportion had lower film solubility and L*-values (lightness) but higher b*-values (yellowness) and ΔE*-values (total color difference) (p<0.05). Electrophoretic study revealed that disulfide bond was present in MPI and CG/MPI blend films. However, hydrogen bonds between CG and MPI in the film matrix were dominant, as elucidated from FTIR spectroscopic analysis. Moreover, thermal stability of CG/MPI blend film was improved as compared to that of films from respective single proteins. Differential scanning calorimetry result suggested solid-state morphology of CG/MPI (6:4) blend film that consisted of amorphous phase of partially miscible CG/MPI mixture and the coexisting two different order phases of individual CG and MPI domains. Thus, the incorporation of MPI into gelatin film could improve the properties of resulting blend film, which were governed by CG/MPI ratio.