RESUMEN
Antibody characterization is essential for understanding the immune system and development of diagnostics and therapeutics. Current technologies are mainly focusing on the detection of antigen-specific immunoglobulin G (IgG) using bulk singleplex measurements, which lack information on other isotypes and specificity of individual antibodies. Digital immunoassays based on nucleic acid amplification have demonstrated superior performance by allowing the detection of single molecules in a multiplex and sensitive manner. In this study, we demonstrate for the first time an immuno-rolling circle amplification (immuno-RCA) assay for the multiplex detection of three antigen-specific antibody isotypes (IgG, IgA, and IgM) and its integration with microengraving. To validate this approach, we used the autoimmune disease immune-mediated thrombotic thrombocytopenic purpura (iTTP) as the model disease with anti-ADAMTS13 autoantibodies as the diagnostic target molecules. To identify the anti-ADAMTS13 autoantibody isotypes, we designed a pool of three unique antibody-oligonucleotide conjugates for identification and subsequent amplification and visualization via RCA. To validate this approach, we first confirmed an assay specificity of >88% and a low limit of detection of 0.3 ng/mL in the spiked buffer. Subsequently, we performed a dilution series of an iTTP plasma sample for the multiplex detection of the three isotypes with higher sensitivity compared to an enzyme-linked immunosorbent assay. Finally, we demonstrated single-cell analysis of human B cells and hybridoma cells for the detection of secreted antibodies using microengraving and achieved a detection of 23.3 pg/mL secreted antibodies per hour. This approach could help to improve the understanding of antibody isotype distributions and their roles in various diseases.
Asunto(s)
Autoanticuerpos , Púrpura Trombocitopénica Trombótica , Antígenos , Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunoglobulina GRESUMEN
Autoantibodies are key biomarkers in clinical diagnosis of autoimmune diseases routinely detected by enzyme-linked immunosorbent assays (ELISAs). However, the complexity of these assays is limiting their use in routine diagnostics. Fiber optic-surface plasmon resonance (FO-SPR) can overcome these limitations, but improved surface chemistries are still needed to guarantee detection of autoantibodies in complex matrices. In this paper, we describe the development of an FO-SPR immunoassay for the detection of autoantibodies in plasma samples from immune-mediated thrombotic thrombocytopenic purpura (iTTP) patients. Hereto, hexahistidine-tagged recombinant ADAMTS13 (rADAMTS13-His6) was immobilized on nitrilotriacetic acid (NTA)-coated FO probes chelated by cobalt (Co(III)) and exposed to anti-ADAMTS13 autoantibodies. Initial studies were performed to optimize rADAMTS13-His6 immobilization and to confirm the specificity of the immunoassay for detection of anti-ADAMTS13 autoantibodies with FO-SPR. The performance of the immunoassay was then evaluated by comparing Co(III)- and nickel (Ni(II))-NTA stabilized surfaces, confirming the stable immobilization of the antigen in Co(III)-NTA-functionalized FO probes. A calibration curve was prepared with a dilution series of a cloned human anti-ADAMTS13 autoantibody in ADAMTS13-depleted plasma resulting in an average interassay coefficient of variation of 7.1% and a limit of detection of 0.24 ng/mL. Finally, the FO-SPR immunoassay was validated using seven iTTP patient plasma samples, resulting in an excellent correlation with an in-house-developed ELISA (r = 0.973). In summary, the specificity and high sensitivity in combination with a short time-to-result (2.5 h compared to 4-5 h for a regular ELISA) make the FO-SPR immunoassay a powerful assay for routine diagnosis of iTTP and with extension for any other autoimmune disease.
Asunto(s)
Autoanticuerpos/sangre , Técnicas Biosensibles/métodos , Cobre/química , Ácido Nitrilotriacético/química , Resonancia por Plasmón de Superficie , Proteína ADAMTS13/química , Proteína ADAMTS13/genética , Proteína ADAMTS13/metabolismo , Tecnología de Fibra Óptica , Histidina/genética , Histidina/metabolismo , Humanos , Proteínas Inmovilizadas/química , Proteínas Inmovilizadas/inmunología , Inmunoensayo , Límite de Detección , Oligopéptidos/genética , Oligopéptidos/metabolismo , Púrpura Trombocitopénica Trombótica/diagnósticoRESUMEN
OBJECTIVE: To investigate the ex vivo pro-inflammatory properties of classical and non-classical monocytes as well as myeloid dendritic cells (mDCs) in systemic sclerosis (SSc) patients. METHODS: Spontaneous production of CXCL10, CCL4, CXCL8 and IL-6 was intracellularly evaluated in classical, non-classical monocytes and Siglec-3-expressing mDCs from peripheral blood of SSc patients and healthy controls (HC) through flow cytometry. In addition, production of these cytokines was determined upon toll-like receptor (TLR) 4 plus Interferon-γ (IFN-γ) stimulation. RESULTS: The frequency of non-classical monocytes spontaneously producing CXCL10 was increased in both limited (lcSSc) and diffuse cutaneous (dcSSC) subsets of SSc patients and CCL4 was augmented in dcSSc patients. The proportion of CCL4-producing mDCs was also elevated in dcSSc patients and the percentage of mDCS producing CXCL10 only in lcSSc patients. Upon stimulation, the frequency of non-classical monocytes expressing CXCL8 was increased in both patient groups and mDCs expressing CXCL8 only in lcSSc. Moreover, these parameters in unsupervised clustering analysis identify a subset of patients which are characterized by lung fibrosis and reduced pulmonary function. CONCLUSIONS: These data point towards a role of activated non-classical monocytes and mDCs producing enhanced levels of proinflammatory cytokines in SSc, potentially contributing to lung fibrosis.
Asunto(s)
Quimiocina CCL4/metabolismo , Quimiocina CXCL10/metabolismo , Dendritas/metabolismo , Interleucina-8/metabolismo , Monocitos/metabolismo , Células Mieloides/metabolismo , Esclerodermia Sistémica/metabolismo , Lectina 1 Similar a Ig de Unión al Ácido Siálico/metabolismo , Adulto , Anciano , Citocinas/biosíntesis , Femenino , Humanos , Interferones/metabolismo , Masculino , Persona de Mediana Edad , Fibrosis Pulmonar/metabolismo , Receptor Toll-Like 4/metabolismoRESUMEN
Oxidized phospholipids are known to be key signaling molecules in the onset of several diseases involving inflammation. The aim of this study was to evaluate the effect of oxidized phosphatidylserines (oxPS) in modulating the immune system, through cytokine production. Flow cytometry analysis was used to evaluate the oxPS capacity to induce the expression of different cytokines by monocytes, myeloid dendritic cells (mDCs) and DCs CD14(-/low)CD16(+). oxPS were formed during oxidation induced by the hydroxyl radical. Among the four families of oxPS studied, only oxPS modified in the polar head with formation of a terminal hydroperoxyacetaldehyde upregulated the production of cytokines IL-8 and TNF-α by monocytes and DCs subsets (mDCs and CD14(-/low)CD16(+) DCs). This family of oxPS showed the capacity to upregulate the production of IL-1ß, IL-6, and MIP-1ß from the same type of cells. A significant raise in the percentage of monocytes and dendritic cells producing the studied cytokines was observed, when compared with basal control. Oxidation products modified in the fatty acyl chain did not upregulate TNF and IL-8. oxPS with terminal hydroperoxyacetaldehyde has pro-inflammatory properties. This outcome may help to understand the biological role of phosphatidylserine oxidation products in inflammatory processes and in dysfunctions of immune system.
Asunto(s)
Citocinas/metabolismo , Células Dendríticas/metabolismo , Regulación de la Expresión Génica , Monocitos/metabolismo , Fosfatidilserinas/metabolismo , Adulto , Femenino , Humanos , Peróxido de Hidrógeno/metabolismo , Hierro/metabolismo , Masculino , Oxidación-ReducciónRESUMEN
Antibodies (Abs) are among the most important class of biologicals, showcasing a high therapeutic and diagnostic value. In the global therapeutic Ab market, fully-human monoclonal Abs (FH-mAbs) are flourishing thanks to their low immunogenicity and high specificity. The rapidly emerging field of single-cell technologies has paved the way to efficiently discover mAbs by facilitating a fast screening of the antigen (Ag)-specificity and functionality of Abs expressed by B cells. This review summarizes the principles and challenges of the four key concepts to discover mAbs using these technologies, being confinement of single cells using either droplet microfluidics or microstructure arrays, identification of the cells of interest, retrieval of those cells and single-cell sequence determination required for mAb production. This review reveals the enormous potential for mix-and-matching of the above-mentioned strategies, which is illustrated by the plethora of established, highly integrated devices. Lastly, an outlook is given on the many opportunities and challenges that still lie ahead to fully exploit miniaturized single-cell technologies for mAb discovery.
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Anticuerpos Monoclonales , Antineoplásicos Inmunológicos , Especificidad de Anticuerpos , HumanosRESUMEN
Retrieving single cells of interest from an array of microwells for further off-chip analysis is crucial in numerous biological applications. To this end, several single cell manipulation strategies have been developed, including optical tweezers (OT). OT represent a unique approach for contactless cell retrieval, but their performance is often suboptimal due to nonspecific cell adhesion to the microwell surface. In this study, we focused on improving the surface chemistry of microwell arrays to ensure efficient single cell manipulation using OT. For this purpose, the surface of an off-stoichiometry thiol-ene-epoxy (OSTE+) microwell array was grafted with polyethylene glycol (PEG) molecules with different molecular weights: PEG 360, PEG 500, PEG 2000, and a PEG Mix (an equimolar ratio of PEG 500 and PEG 2000). Contact angle measurements showed that the PEG grafting process resulted in an increased surface energy, which was stable for at least 16 weeks. Next, cell adhesion of two cell types, baker's yeast (Saccharomyces cerevisiae) and human B cells, to surfaces treated with different PEGs was evaluated by registering the presence of cellular motion inside microwells and the efficiency of optical lifting of cells that display motion. Optimal results were obtained for surfaces grafted with PEG 2000 and PEG Mix, reaching an average fraction of cells with motion of over 93% and an average lifting efficiency of over 96% for both cell types. Upon the integration of this microwell array with a polydimethylsiloxane (PDMS) microfluidic channel, PEG Mix resulted in proper washing of non-seeded cells. We further demonstrated the wide applicability of the platform by manipulating non-responding yeast cells to antifungal treatment and B cells expressing surface IgG antibodies. The combination of the optimized microwell surface with continuous microfluidics results in a powerful and versatile platform, allowing high-throughput single cell studies and retrieval of target cells for off-chip analysis.
Asunto(s)
Micromanipulación/instrumentación , Pinzas Ópticas , Polietilenglicoles/química , Análisis de la Célula Individual/instrumentación , Compuestos de Sulfhidrilo/química , Linfocitos B/citología , Adhesión Celular , Células Cultivadas , Compuestos Epoxi/química , Diseño de Equipo , Humanos , Técnicas Analíticas Microfluídicas/instrumentación , Saccharomyces cerevisiae/citología , Propiedades de SuperficieRESUMEN
Cobalt-nitrilotriacetic acid (Co(III)-NTA) chemistry is a recognized approach for oriented patterning of His6-tagged bioreceptors. We have applied the matching strategy for the first time on a surface plasmon resonance (SPR) platform, namely, the commercialized fiber optic (FO)-SPR. To accomplish this, His6-tagged bioreceptor (scFv-33H1F7) and its target PAI-1 were used as a model system, after scrutinizing the specificity of their interaction. When benchmarked to traditional carboxyl-based self-assembled monolayers (SAM), NTA allowed (1) more efficient FO-SPR surface coverage with bioreceptors compared with the former and (2) realization of thus far difficult-to-attain label-free bioassays on the FO-SPR platform in both buffer and 20-fold diluted human plasma. Moreover, Co(III)-NTA surface proved to be compatible with traditional gold nanoparticle-mediated signal amplification in the buffer as well as in 10-fold diluted human plasma, thus expanding the dynamic detection range to low ng/mL. Both types of bioassays revealed that scFv-33H1F7 immobilized on the FO-SPR surface using different concentrations (20, 10, or 5 µg/mL) had no impact on the bioassay sensitivity, accuracy, or reproducibility despite the lowest concentration effectively resulting in close to 20% fewer bioreceptors. Collectively, these results highlight the importance of Co(III)-NTA promoting the oriented patterning of bioreceptors on the FO-SPR sensor surface for securing robust and sensitive bioassays in complex matrices, both in label-free and labeled formats.
Asunto(s)
Técnicas Biosensibles/métodos , Cobalto/química , Nanopartículas del Metal/química , Resonancia por Plasmón de Superficie/métodos , HumanosRESUMEN
BACKGROUND: The biological diagnosis of immune-mediated thrombotic thrombocytopenic purpura (iTTP) is based on determination of ADAMTS13 activity (<10%) and anti-ADAMTS13 autoantibodies. ADAMTS13 antigen levels are not routinely measured in iTTP patients, but studies have shown that antigen levels are a valuable prognostic factor. OBJECTIVES: To (a) report the validation of our in-house developed ADAMTS13 antigen enzyme-linked immunosorbent assay (ELISA) and determine ADAMTS13 antigen in a large cohort of healthy donor and iTTP patient plasma samples; and (b) to investigate whether ADAMTS13 antigen determination is not disturbed by the presence of anti-ADAMTS13 autoantibodies. METHODS: Our in-house ADAMTS13 antigen ELISA was validated in terms of sensitivity, repeatability, and reproducibility. ADAMTS13 antigen levels were determined in plasma samples from 423 healthy donors and 112 acute iTTP patients. Purified IgGs from iTTP patients were added to normal human plasma to determine whether anti-ADAMTS13 autoantibodies hampered ADAMTS13 antigen determination. RESULTS: Our in-house ADAMTS13 antigen ELISA has a detection limit of 3% and low intra-assay (coefficient of variation, %CV < 10%) and inter-assay (%CV < 18%) variability. ADAMTS13 antigen levels were significantly reduced (P < .0001) in acute iTTP patients (15 ± 18%) compared to healthy donors (101 ± 18%). The anti-ADAMTS13 autoantibodies in plasma of iTTP patients did not impede ADAMTS13 antigen determinations using our in-house ELISA. CONCLUSIONS: Our in-house ADAMT13 antigen ELISA is a powerful tool to correctly determine ADAMTS13 antigen levels in iTTP patients, which supports routine ADAMTS13 antigen measurements in these patients to have better insight into disease prognosis.
Asunto(s)
Púrpura Trombocitopénica Idiopática , Púrpura Trombocitopénica Trombótica , Proteína ADAMTS13 , Autoanticuerpos , Ensayo de Inmunoadsorción Enzimática , Humanos , Púrpura Trombocitopénica Trombótica/diagnóstico , Reproducibilidad de los ResultadosRESUMEN
Akt2 is a pivotal player in a complex web of signaling pathways controlling cell growth, proliferation, and survival. The deregulation or aberrations of Akt2 have been associated with tumor progression, metastatic spread, and, lastly, chemoresistance. The impairment of its activity has gained more attention because Akt2 is intertwined with a range of signaling paths, including the Phosphatidylinositol 3 kinase/Akt/Mammalian target of rapamycin (PI3K/mTOR) signaling axis, which are involved in macromolecules synthesis and metabolism. Here, we focus on Akt2 because of its involvement in the acquisition of stem cell-like properties, responsible for invasiveness and chemoresistance, also promoted by Twist. We also suggest therapeutic strategies targeting Akt2 to overcome the drawbacks of current cancer therapies.
Asunto(s)
Neoplasias de la Mama/patología , Proteínas Nucleares/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Proteína 1 Relacionada con Twist/metabolismo , Animales , Antineoplásicos/farmacología , Neoplasias de la Mama/tratamiento farmacológico , Proliferación Celular/fisiología , Supervivencia Celular/fisiología , Resistencia a Antineoplásicos , Femenino , Humanos , Metástasis de la Neoplasia , Transducción de Señal/fisiologíaRESUMEN
The expression of CD44 tags cells with stemness-associated properties (cancer initiating cells or cancer stem cells - CSC). This membrane glycoprotein with a cytoplasmic domain indirectly associated with the cellular cytoskeleton, has a crucial role in tumorigenesis. The CD44 receptor enables the cell to respond to changes in tumor microenvironment, promoting several signaling events related to tumor initiation, progression and fixation in distant host tissues. Although the contribution of this transmembrane protein in gene regulation remains unclear, its overexpression in adenocarcinomas, mostly supported by microRNA (miR)-mediated upregulation of target mRNA, is widely accepted. Herein, we gather the evidence that CD44 is one of the most predominant markers of malignant cells and may be found in diverse phenotypes associated with tumor progression. Additionally, CD44 tumor receptors were found to have different roles at a transcriptional level. Thus, innovative therapeutic strategies should rely heavily on its metastasis-promoting ability. Furthermore, the concept of selectively targeting cell sub-populations may be used to develop specific therapeutic and/or diagnostic systems. An approach based on targeting CD44⺠cells might provide a strategy to design guided-therapeutic systems against multiple malignant cells including putative CSC.
Asunto(s)
Antineoplásicos/uso terapéutico , Medicina Basada en la Evidencia , Receptores de Hialuranos/metabolismo , Terapia Molecular Dirigida , Neoplasias/tratamiento farmacológico , Células Madre Neoplásicas/efectos de los fármacos , Medicina de Precisión , Animales , Biomarcadores/sangre , Biomarcadores/metabolismo , Transición Epitelial-Mesenquimal/efectos de los fármacos , Humanos , Receptores de Hialuranos/sangre , Receptores de Hialuranos/química , Proteínas de Neoplasias/antagonistas & inhibidores , Proteínas de Neoplasias/sangre , Proteínas de Neoplasias/metabolismo , Neoplasias/sangre , Neoplasias/metabolismo , Neoplasias/patología , Células Madre Neoplásicas/metabolismo , Células Madre Neoplásicas/patologíaRESUMEN
Babesia ovis is a tick-transmitted protozoa parasite that infects small ruminants causing fever, anaemia, hemoglobinuria, anorexia and, in acute cases, death. Common in tropical and sub-tropical areas, the presence of this parasite in sheep herds has an economic impact on industry and therefore sensitive methods for the diagnosis and disease eradication are required. To achieve this goal, a semi-nested PCR for B. ovis specific identification was developed and consequent reaction conditions and enzymes were optimized and tested with field samples. 300 blood samples from small ruminants and 39 ticks from Rhipicephalus genus were collected from different regions of Portugal. Afterwards, DNA extraction was performed and conventional and semi-nested PCR were accomplished for all samples. The results obtained from both methodologies were compared and the sensitivity was evaluated. Employing the semi-nested PCR it was possible to identify a higher number of positive cases among the evaluated samples than using the conventional PCR, namely 38/300 blood samples and 7/39 ticks. However, fragment amplification was only observed in 5 out of 300 blood samples and in none of the 39 ticks when a conventional PCR was employed. The validation of the results was achieved by sequencing the DNA fragments corresponding to the hypervariable v4 region of the 18S ribosomal RNA gene and performing an alignment with sequences already published on GenBank(®). The ticks collected in this study belong to the Rhipicephalus genus, although other species could be involved as a vector in the Babesia spread. The diagnostic assay here described is presently the most effective and sensitive method for detection of B. ovis in field blood samples and ticks, enabling the detection up to 1 parasite into 10(9) erythrocytes.
Asunto(s)
Babesia/aislamiento & purificación , Babesiosis/diagnóstico , Enfermedades de las Cabras/diagnóstico , Reacción en Cadena de la Polimerasa/veterinaria , Rhipicephalus/parasitología , Enfermedades de las Ovejas/diagnóstico , Animales , Vectores Arácnidos/parasitología , Babesia/genética , Babesiosis/parasitología , Secuencia de Bases , ADN Protozoario/genética , ADN Ribosómico/genética , Enfermedades de las Cabras/parasitología , Cabras , Datos de Secuencia Molecular , Portugal , ARN Ribosómico 18S/genética , Sensibilidad y Especificidad , Análisis de Secuencia de ADN/veterinaria , Ovinos , Enfermedades de las Ovejas/parasitologíaRESUMEN
Farnesol, a compound widely found in several agro-food by-products, is an important bioactive agent that can be exploited in cosmetics and pharmaceutics but the direct bioapplication of this compound is limited by its volatility. Here the entrapment of farnesol in silica capsules was investigated to control the release of this bioactive compound in the vapor phase and in ethanol solutions. The preparation of silica capsules with oil cores was obtained by employing a sol-gel method using O/W/O multiple emulsions. Two distinct chemical vehicles for farnesol have been investigated, retinol and oleic acid, that afterwards have been emulsified as internal oil phases. The volatile release of farnesol from the as-prepared SiO2 capsules was investigated by headspace solid phase microextraction (HS-SPME) followed by gas chromatographic analysis (GC), and the release to ethanol was carried out by direct injection of the ethanolic fraction into the GC system. It is demonstrated that these capsules are efficient for the long controlled release of farnesol and that the respective profiles depend on the chemical parameters employed in the synthesis of the capsules.
RESUMEN
Objetivo: Evaluar y analizar la composición del thiner del DF y compararlo con una muestra de thiner extranjero (USA) como parte de una evaluación química y toxicológica, y reportar las manifestaciones oftalmológicas de inhaladores crónicos de thiner Método: Para el análisis del thiner se utilizó un cromatógrafo de gases marca Perkin-Elmer, modelo sigma 300 equipado con detector de ionización de flama, y los potenciales visuales evocados con equipo Nicolet Path finder II. Con. método prospectivo de 1983-1996 se hizo estudio oftalmológico completo de 40 inhaladores crónicos de thiner. Resultados: En las muestras de thiner de México, se identificaron 20 elementos logrando una separación cuantitativa del 93.96 por ciento al 100 por ciento, predominando el tolueno con el 79.48 por ciento, y en el thiner de USA se identificaron 9 elementos con mayor concentración de metilpropilcetona 21.44 por ciento (cuadro 1). Tiempo de inhalar thiner de 3-15 años, edad 18-38 años, promedio 26 años, razón hombre/mujer 10:1, agudeza visual final de 20/200 o menor en el 84 por ciento, discromatopsia total en el 84 por ciento, con alteraciones campimétricas de sólo islotes de visión periférica en el 87 por ciento, midriasis en el 84 por ciento, con arreflexia a la luz y conservación del punto próximo de convergencia. Oftalmoscópicamente en la fase aguda neurorretinitis generalizada, y en la fase crónica atrófia óptica, degeneración retiniana difusa con envainamiento arteriolar y vasos fantasmas. Conclusiones: El thiner es una mezcla variada de solventes tóxicos, su inhalación crónica afecta a largo plazo nervio óptico y retina, con un daño gradual, bilateral e irreversible de predominio arteriolar con envenenamiento y vasos fantasmas. Las alteraciones oftalmoscópicas y la midriasis con la disociación pupilar "Luz-Acomodación-Convergencia" podría ser una orientación para el clínico en la sospecha de esta toxicomanía