Physical Exercise-Induced DNA Methylation in Disease-Related Genes in Healthy Adults-A Systematic Review With Bioinformatic Analysis.

ABSTRACT: Vasileva, F, Hristovski, R, Font-Lladó, R, Georgiev, G, Sacot, A, López-Ros, V, Calleja-González, J, Barretina-Ginesta, J, López-Bermejo, A, and Prats-Puig, A. Physical exercise-induced DNA methylation in disease-related genes in healthy adults-A systematic review with bioinformatic analysis. J Strength Cond Res 38(2): 384-393, 2024-This study aimed to systematically review the existing literature regarding physical exercise (PE) and DNA methylation (DNAm) in healthy adults. Specific goals were to (a) identify differently methylated genes (DMGs) after PE intervention, their imprinting status, chromosome and genomic location, function, and related diseases; and (b) to screen for core genes and identify methylation changes of the core genes that can be modified by PE intervention. Our search identified 2,869 articles from which 8 were finally included. We identified 1851 DMGs ( p < 0.05) after PE intervention, although 45 of them were imprinted. Aerobic exercise (AE) seems to induce more DNA hypermethylation rather than hypomethylation, whereas anaerobic exercise (AN) seems to induce more DNA hypomethylation rather than hypermethylation. Aerobic exercise induced highest % of methylation changes on chromosome 6, whereas AN and mixed type (MT) on chromosome 1. Mixed type induced higher % of methylation changes close to transcription start site in comparison to AE and AN. After PE intervention, DMGs were mainly involved in fat metabolism, cell growth, and neuronal differentiation, whereas diseases regulated by those genes were mainly chronic diseases (metabolic, cardiovascular, neurodegenerative). Finally, 19 core genes were identified among DMGs, all related to protein metabolism. In conclusion, our findings may shed some light on the mechanisms explaining PE-induced health benefits such as the potential role that PE-induced DNAm may have in disease prevention and disease treatment.

From microscopic to macroscopic sports injuries. Applying the complex dynamic systems approach to sports medicine: a narrative review.

A better understanding of how sports injuries occur in order to improve their prevention is needed for medical, economic, scientific and sports success reasons. This narrative review aims to explain the mechanisms that underlie the occurrence of sports injuries, and an innovative approach for their prevention on the basis of complex dynamic systems approach. First, we explain the multilevel organisation of living systems and how function of the musculoskeletal system may be impaired. Second, we use both, a constraints approach and a connectivity hypothesis to explain why and how the susceptibility to sports injuries may suddenly increase. Constraints acting at multiple levels and timescales replace the static and linear concept of risk factors, and the connectivity hypothesis brings an understanding of how the accumulation of microinjuries creates a macroscopic non-linear effect, that is, how a common motor action may trigger a severe injury. Finally, a recap of practical examples and challenges for the future illustrates how the complex dynamic systems standpoint, changing the way of thinking about sports injuries, offers innovative ideas for improving sports injury prevention.

Free Play with Certain Equipment Constrains the Emergence of Exploratory Behavior and Physical Activity in Preschoolers.

The aim of this study was to establish whether various types of play equipment differentially constrain exploratory behavior and spontaneous physical activity patterns in preschoolers when they play freely with them. Fourteen children, 3-4 years old, played with four different types of equipment. The children wore an accelerometer and data were analyzed taking into account overall physical activity, the time spent at different intensity levels of move-ment, and the entropy of the time series. All trials were video-recorded and a systematic observation instrument was used to register actions and interactions with partners and equipment. The data were subsequently analyzed by means of principal component analysis (PCA) and the dynamic overlap order parameter, to identify the rate and breadth of exploratory behavior on different time scales. Our results revealed that the children were physically less active when playing with portable and small equipment. The PCA showed that when playing without equipment, the children usually played standardized games, like playing tag, in groups. Movement patterns were more or less varied depending on the equip-ment, and thus some equipment constrained the emergence of different types of games or motor behavior.

Timescales for exploratory tactical behaviour in football small-sided games.

The aim of this study was to identify the dynamics of tactical behaviour emerging on different timescales in football small-sided games and to quantify short- and long-term exploratory behaviour according to the number of opponents. Two teams of four professional male footballers played small-sided games against two different teams with a variable number of opponents (3, 5 and 7). Data were collected using a combination of systematic observation and a non-differential global positioning system (15 Hz). The temporal diversity and structural flexibility of the players were determined by calculating the dynamic overlap order parameter q, entropy and trapping strength. Analysis of the exploratory dynamics revealed two different timescales, forming a different metastable landscape of action for each constraint. Fast dynamics lasted on average a few seconds and consisted of changes in tactical patterns. The long timescale corresponded to the shared tasks of offence and defence lasting tens of seconds. The players' tactical diversity decreased with an increasing number of opponents, especially in defence. Manipulating numerical imbalance is likely to promote changes in the diversity, unpredictability and flexibility of tactical solutions. The fact that the temporally nested structure of constraints shaped the emergence of tactical behaviour provides a new rationale for practice task design. The manipulation of numerical imbalance on the timescale of a few tens of seconds, on which the exploratory behaviour of players saturates, may help coaches to optimise the exploratory efficiency of the small-sided games.

Intentional thought dynamics during exercise performed until volitional exhaustion.

Using a non-linear approach, intentional dynamics of thoughts were examined during constant cycling performed until volitional exhaustion. Participants (n = 12) completed two sessions at 80% Wmax. Their (1) intrinsic thought dynamics (i.e., no-imposed thoughts condition) and (2) intentional thought dynamics (i.e., imposed task-unrelated thoughts condition; TUT) were recorded and then classified into four categories: internal and external TUT (TUT-I, TUT-E) and external and internal task-related thoughts (TRT-E, TRT-I). The probability estimates for maintaining each thought category stable, the rate of switching from one category to another, and the entropy dynamics along the testing procedure were assessed and compared through time phase. Friedman ANOVA tests revealed a significant effect of effort increase on thought contents only in the imposed TUT test. While TUT-I probabilities decreased significantly (P < .001) as effort increased, TRT-I probabilities increased (P < .05). Moreover, the entropy to the entire thought dynamics increased at the outset of task performance and decreased upon approaching volitional exhaustion (P < .001). As time spent in constant effort increased, and volitional exhaustion approached, task relatedness (TUT, TRT), direction (internal, external), and entropy of thought contents changed unintentionally providing further evidence for a nonlinear dynamics of attention focus.

Integrative Proposals of Sports Monitoring: Subjective Outperforms Objective Monitoring.

Current trends in sports monitoring are characterized by the massive collection of tech-based biomechanical, physiological and performance data, integrated through mathematical algorithms. However, the application of algorithms, predicated on mechanistic assumptions of how athletes operate, cannot capture, assess and adequately promote athletes' health and performance. The objective of this paper is to reorient the current integrative proposals of sports monitoring by re-conceptualizing athletes as complex adaptive systems (CAS). CAS contain higher-order perceptual units that provide continuous and multilevel integrated information about performer-environment interactions. Such integrative properties offer exceptional possibilities of subjective monitoring for outperforming any objective monitoring system. Future research should investigate how to enhance this human potential to contribute further to athletes' health and performance. This line of argument is not intended to advocate for the elimination of objective assessments, but to highlight the integrative possibilities of subjective monitoring.

Network Physiology of Exercise: Beyond Molecular and Omics Perspectives.

Molecular Exercise Physiology and Omics approaches represent an important step toward synthesis and integration, the original essence of Physiology. Despite the significant progress they have introduced in Exercise Physiology (EP), some of their theoretical and methodological assumptions are still limiting the understanding of the complexity of sport-related phenomena. Based on general principles of biological evolution and supported by complex network science, this paper aims to contrast theoretical and methodological aspects of molecular and network-based approaches to EP. After explaining the main EP challenges and why sport-related phenomena cannot be understood if reduced to the molecular level, the paper proposes some methodological research advances related to the type of studied variables and measures, the data acquisition techniques, the type of data analysis and the assumed relations among physiological levels. Inspired by Network Physiology, Network Physiology of Exercise provides a new paradigm and formalism to quantify cross-communication among diverse systems across levels and time scales to improve our understanding of exercise-related phenomena and opens new horizons for exercise testing in health and disease.

Constraints-induced emergence of functional novelty in complex neurobiological systems: a basis for creativity in sport.

In this paper we present a model of creativity captured as exploration and production of novel and functionally efficient behaviors, based on the statistical mechanics of disordered systems. In support of the modelling, we highlight examples of creative behaviors from our research in sports like boxing and rugby union. Our experimental results show how manipulation of practice task constraints changes the exploratory breadth of the hierarchically soft-assembled action landscape. Because of action metastability and differing task constraints, the specificity of each assembled movement configuration is unique. Empirically, a movement pattern's degree of novelty may be assessed by the value of the order parameter describing action. We show that creative and adaptive movement behavior may be induced by at least two types of interven-tions, based on relaxing task constraints which we term direct and indirect. Direct relaxing is typically a function of changing task constraints so that the number of affordances that can satisfy goal constraints increases. Indirect relaxing of constraints occurs when a habitual action is suppressed by, for example, stringent instructional constraints during sports training. That suppression simultaneously relaxes other correlated constraints that enable larger exploratory capacity and new affordances to emerge for the athlete or team.

Constraints-controlled metastable dynamics of exercise-induced psychobiological adaptation.

A fundamental question in the theory of psychobiological adaptation and specifically of sports training is the problem of how adaptation to sports performance demands occurs as a consequence of systematic exercise. In this position paper, we review some results of our previous and current research conducted on several different levels of exercise-induced effects. Based on these results, we contend that the control of psychobiological systems during exercise is constraints based. Constraints direct the flow of behavioral changes on a rugged metastable landscape. Such adaptive behavior is soft-assembled, consisting of context-sensitive cooperative configurations of system components that dwell on different time scales.

Theory of Cooperative-Competitive Intelligence: Principles, Research Directions, and Applications.

We present a theory of cooperative-competitive intelligence (CCI), its measures, research program, and applications that stem from it. Within the framework of this theory, satisficing sub-optimal behavior is any behavior that does not promote a decrease in the prospective control of the functional action diversity/unpredictability (D/U) potential of the agent or team. This potential is defined as the entropy measure in multiple, context-dependent dimensions. We define the satisficing interval of behaviors as CCI. In order to manifest itself at individual or team level, this capacity harnesses properties such as degeneracy, pleiotropy (pluri-potentiality), synergies, and metastability. Intelligence is embodied because intelligent behavior is deeply dependent on body functionalities, defined as entropy measures. We base our theory on three principles: (a) relativity of functional entropy/information in agent (team)-environment systems, (b) tendency toward the satisficing level of D/U potential, and (c) tendency toward the non-decreasing D/U potential. The conjunction of these three principles provides existence of sub-optimal behaviors associated with CCI. First, we deal with the problem of how to reduce multidimensional behavior to a concept that accounts for the vast set of scenarios in which CCI is manifested. Secondly, we define and discuss the three interacting principles that underpin CCI behavior as well as providing an outline for a future CCI research program supported by agent-based modeling and empirical research. Finally, we provide some preliminary practical issues that stem from the theory.

Interlimb Coordination: A New Order Parameter and a Marker of Fatigue During Quasi-Isometric Exercise?

Although exercise-induced fatigue has been mostly studied from a reductionist and component-dominant approach, some authors have started to test the general predictions of theories of self-organized change during exercises performed until exhaustion. However, little is known about the effects of fatigue on interlimb coordination in quasi-isometric actions. The aim of this study was to investigate the effect of exercise-induced fatigue on upper interlimb coordination during a quasi-isometric exercise performed until exhaustion. In order to do this, we hypothesized an order parameter that governs the interlimb coordination as an interlimb correlation measure. In line with general predictions of theory of phase transitions, we expected that the locally averaged values of the order parameter will increase as the fatigue driven system approaches the point of spontaneous task disengagement. Seven participants performed a quasi-isometric task holding an Olympic bar maintaining an initial elbow flexion of 90 degrees until fatigue induced spontaneous task disengagement. The variability of the elbow angle was recorded through electrogoniometry and the obtained time series were divided into three segments for further analysis. Running correlation function (RCF) and adopted bivariate phase rectified signal averaging (BPRSA) were applied to the corresponding initial (30%) and last (30%) segments of the time series. The results of both analyses showed that the interlimb correlation increased between the initial and the final segments of the performed task. Hence, the hypothesis of the research was supported by evidence. The enhancement of the correlation in the last part means a less flexible coordination among limbs. Our results also show that the high magnitude correlation (%RCF > 0.8) and the %Range (END-BEG) may prove to be useful markers to detect the effects of effort accumulation on interlimb coordination. These results may provide information about the loss of adaptability during exercises performed until exhaustion. Finally, we briefly discuss the hypothesis of the inhibitory percolation process being the general explanation of the spontaneous task disengagement phenomenon.

Network Physiology of Exercise: Vision and Perspectives.

The basic theoretical assumptions of Exercise Physiology and its research directions, strongly influenced by reductionism, may hamper the full potential of basic science investigations, and various practical applications to sports performance and exercise as medicine. The aim of this perspective and programmatic article is to: (i) revise the current paradigm of Exercise Physiology and related research on the basis of principles and empirical findings in the new emerging field of Network Physiology and Complex Systems Science; (ii) initiate a new area in Exercise and Sport Science, Network Physiology of Exercise (NPE), with focus on basic laws of interactions and principles of coordination and integration among diverse physiological systems across spatio-temporal scales (from the sub-cellular level to the entire organism), to understand how physiological states and functions emerge, and to improve the efficacy of exercise in health and sport performance; and (iii) to create a forum for developing new research methodologies applicable to the new NPE field, to infer and quantify nonlinear dynamic forms of coupling among diverse systems and establish basic principles of coordination and network organization of physiological systems. Here, we present a programmatic approach for future research directions and potential practical applications. By focusing on research efforts to improve the knowledge about nested dynamics of vertical network interactions, and particularly, the horizontal integration of key organ systems during exercise, NPE may enrich Basic Physiology and diverse fields like Exercise and Sports Physiology, Sports Medicine, Sports Rehabilitation, Sport Science or Training Science and improve the understanding of diverse exercise-related phenomena such as sports performance, fatigue, overtraining, or sport injuries.

Training or Synergizing? Complex Systems Principles Change the Understanding of Sport Processes.

There is a need to update scientific assumptions in sport to promote the critical thinking of scientists, coaches, and practitioners and improve their methodological decisions. On the basis of complex systems science and theories of biological evolution, a systematization and update of theoretical and methodological principles to transform the understanding of sports training is provided. The classical focus on learning/acquiring skills and fitness is replaced by the aim of increasing the diversity/unpredictability potential of teams/athletes through the development of synergies. This development is underpinned by the properties of hierarchical organization and circular causality of constraints, that is, the nestedness of constraints acting at different levels and timescales. These properties, that integrate bottom-up and top-down all dimensions and levels of performance (from social to genetic), apply to all types of sport, ages, or levels of expertise and can be transferred to other fields (e.g., education, health, management). The team as the main training unit of intervention, the dynamic concept of task representativeness, and the co-adaptive and synergic role of the agents are some few practical consequences of moving from training to synergizing.

Dynamics of multi-articular coordination in neurobiological systems.

Although previous work in nonlinear dynamics on neurobiological coordination and control has provided valuable insights from studies of single joint movements in humans, researchers have shown increasing interest in coordination of multi-articular actions. Multi-articular movement models have provided valuable insights on neurobiological systems conceptualised as degenerate, adaptive complex systems satisfying the constraints of dynamic environments. In this paper, we overview empirical evidence illustrating the dynamics of adaptive movement behavior in a range of multi-articular actions including kicking, throwing, hitting and balancing. We model the emergence of creativity and the diversity of neurobiological action in the meta-stable region of self organising criticality. We examine the influence on multi-articular actions of decaying and emerging constraints in the context of skill acquisition. We demonstrate how, in this context, transitions between preferred movement patterns exemplify the search for and adaptation of attractor states within the perceptual motor workspace as a function of practice. We conclude by showing how empirical analyses of neurobiological coordination and control have been used to establish a nonlinear pedagogical framework for enhancing acquisition of multi-articular actions.

On the Relatedness and Nestedness of Constraints.

The purpose of this opinion paper is providing a platform for explaining and discussing the relatedness and nestedness of constraints on the basis of four claims: (a) task constraints are distributed between the person and the environment and hence are relational variables, (b) being relational, task constraints are also emergent properties of the organism/environment system, (c) constraints are nested in timescales, and (d) a vast set of constraints are correlated through circular causality. Theoretical implications for improving the understanding of the constraints-led approach and practical applications for enhancing the manipulation of constraints in learning and training settings are proposed.

Flow as an Embodied State. Informed Awareness of Slackline Walking.

Flow during exercise has been theorized and studied solely through subjective-retrospective methods as a "scull bound" construct. Recent advances of the radical embodied perspectives on conscious mind and cognition pose challenges to such understanding, particularly because flow during exercise is associated with properties of performer's movement behavior. In this paper we use the concept of informed awareness to reconceptualize flow experience as a property of the performer-environment coupling, and study it during a slackline walking task. To empirically check the possible relatedness of the behavior-experience complementary pair, two measures were considered. The experiential realm was quantified by the flow short scale and the behavioral realm by the Hurst (H) exponent obtained through accelerometry time series of the legs and the center of body mass (CoM). In order to obtain a coarse-grained insight about the degree of co-varying within the perception-action flow of performers, we conducted correlational and multiple regression analyses. Measures of behavioral variables (H exponents of the dominant, subdominant leg and the CoM, were treated as explanatory, and the flow scale and its subscale (fluency of movements and absorption) scores asresponse variables containing summarized information about perceptual experiences of performers. In order to check for possible mediating or confounding effects of training parameters on the action-perception variables' covariance, we included two additional variables which measured the degree of engagement of participants with the task. Results revealed that the temporal structure of fluctuations of the dominant leg, as measured by the Hurst exponent, was a strong mediator of effects of training variables and the subdominant leg fluctuations, on the flow scale and the subscale scores. The magnitude of Hurst exponents of both legs was informative about the degree of stability within the performer-environment system. The degree of critical slowing down, as measured by Hurst exponents, consistently co-varied with the flow scale and subscales. The experience of flow during the slackline walking task was dominantly saturated by the perceived fluency of movements and less so by the absorption experience. The stable co-variance of perception-action variables signified the embodied nature of the flow experience.

Effects of Temporary Numerical Imbalances on Collective Exploratory Behavior of Young and Professional Football Players.

The aim of this study was to explore how the use of temporary numerical imbalances during small-sided Game SSGs affects team's exploratory behaviors (i.e., variety and quantity of responses given in an ever-changing game context and its rate of change) in different age groups. Two different age groups (under-15 and under-23) of football players participated in the study. For each age group, three teams of five players played six small-sided games of 5 min duration in different conditions: (i) numerical balance (GK + 4 vs. 4 + GK); (ii) temporary numerical imbalance, which consisted of a numerical change of teammates and opponents every one minute. Latitude and longitude GPS coordinates were used to determine the positioning-derived variables. The dynamic overlap (i.e. the measure of average similarity of the game patterns that take place in increasingly larger time intervals) was used to provide information of the rate and breadth of exploratory behavior. The results revealed that the long-term exploratory breadth increased for the under-23 age group. Non-clear effects were found for the short-term rate of exploration, but with an increasing trend. In the under-15 group, the exploratory behavior was more likely to increase in the long term. The increase for the short-term rate of exploration was unclear, but it follows an increasing trend. These results suggest that the use of temporary numerical imbalances could offer coaches more dynamic training situations and different adaptive training environments similar to matches.

Physiological- and performance-related effects of acute olive oil supplementation at moderate exercise intensity.

BACKGROUND: The consumption of olive oil is associated with a diminished risk of cardiovascular disorders and mortality, but the impact of olive oil supplementation on endurance performance is still unclear. Since the beneficial effects of olive oil are observed at a systemic level, its effectiveness may not be precisely measured through the commonly registered maximal and threshold values of some physiological and performance parameters. In contrast, we suggest evaluating it through variables able to capture the coordinated behaviour of physiological systems. Thus, the aim of the current research was to assess the effect of an acute extra virgin olive oil supplementation on cardiorespiratory coordination (CRC) and performance, compared to palm oil. METHODS: Three separate effort test sessions were carried out separated by 7-day interval. During each session, participants (n = 7) repeated the same progressive and maximal walking test, but under different dietary supplementations in a randomized order: (1) olive oil, (2) palm oil, and (3) placebo. A principal component (PC) analysis of selected cardiovascular and cardiorespiratory variables was carried out to evaluate CRC. Eigenvalues of the first PC (PC1) and the loadings of the cardiorespiratory variables onto PC1 were compared among dietary supplementations. In order to more accurately evaluate CRC, all the tests were divided into 3 equal sections, corresponding to low, moderate, and high exercise intensities, and the aforementioned procedure was repeated for each section in all the tests. RESULTS: Statistically significant differences were observed regarding PC1 eigenvalues among dietary supplementations (χ2 (8,2) = 6.3; p = .04), only at moderate intensity exercise. Specifically, PC1 eigenvalues were higher under olive oil compared to palm oil (2.63 ± 0.51 vs. 2.30 ± 0.28; Z = 2.03; p = .04; d = 0.80) and placebo supplementations (2.63 ± 0.51 vs. 2.38 ± 0.36; Z = 2.20; p = .03; d = 0.57). CONCLUSIONS: Supplementation with extra virgin olive oil increased CRC during a progressive walking test at moderate intensity, although did not change performance and other physiological markers. CRC analysis appears as a sensitive tool to investigate the physiological and performance effects of dietary supplementations.

Principal component analysis as a novel approach for cardiorespiratory exercise testing evaluation.

OBJECTIVE: Our purpose was to apply a principal component analysis (PCA) approach to cardiorespiratory exercise to test evaluation and its sensitivity to workload accumulation. APPROACH: Twenty-five healthy young adults performed a progressive and maximal cycling test, which was divided into two parts: moderate and high workload intensities, using a ventilatory threshold as a cut point. A PCA of the time series of cardiovascular and respiratory variables was performed in each part and the number of principal components (PCs), the eigenvalues of the first PC (PC1), and the information entropy were calculated. MAIN RESULTS: The number of PCs increased, the eigenvalues of PC1 decreased (t = 5.32; p  < 0.001; d = 1.39) and entropy was significantly higher (Z = 3.10; p  = .002; d = 1.16) at high workload intensities, compared to moderate intensities. SIGNIFICANCE: Results showed the sensitivity of the PCA approach to workload accumulation and corroborates its potential for improving the evaluation and interpretation of cardiorespiratory exercise testing. In particular, it points to being a good candidate to objectively detect qualitative changes or thresholds.