The Influence of Trunk Impairment Level on the Kinematic Characteristics of Alpine Sit-Skiing: A Case Study of Paralympic Medalists.

This study aimed to examine the relationship between the trunk impairment level and the trunk kinematic characteristics during alpine sit-skiing from a classification perspective. Three Paralympic medalists in sitting classes (LW10-2, LW11, and LW12-2) participated in the present study. To simulate the racing conditions, giant slalom gates were set. To measure the kinematics of the skier and sit-ski during skiing, a motion capture method with inertial measurement units was used. The muscle activities of the trunk muscles were evaluated using electromyography. Chest lateral flexion, chest flexion, and hip flexion/extension angle during sit-skiing were reduced due to impairment. Additionally, the insufficient lateral flexion (angulation) caused a decrease in edging angle, and that the insufficient chest and hip flexion/extension caused a lower loading in the latter half of the turn through smaller vertical movement. Since edging angle and loading are key factors in ski control, the three joint motions could be measures of sport-specific activity limitation in sit-skiing classification. Between the LW10-2 and LW11 skiers, no distinct differences in trunk kinematics were found. Assuming the scaling factor of race time as a measure of skiing performance, one possible reason is that the difference in skiing performance the LW10-2 and LW11 skiers is considerably smaller relative to differences between the LW11 and LW12-2 skiers. There were no distinct differences among classes in the results of muscle activity, and therefore, this information appears to play a minimal role for classification.

Muscle Activity and Morphology in Slalom Skiing by a Single-Leg Amputee Ski Racer: A Case Study of a Paralympic Athlete.

The aim of this study was to clarify the characteristics of skiing by a single-leg amputee ski racer from the viewpoints of muscle activity, morphology, and the relationship between both elements through comparisons with those of a non-disabled ski racer. One elite athlete, classified as LW2 (left thigh amputation), and one non-disabled athlete, as a control, participated in this study. The cross-sectional area of thigh muscles was measured through magnetic resonance imaging. Additionally, muscle activities and joint and segment kinematics during slalom skiing were measured using electromyography and inertial measurement units, respectively. The muscle activities and joint kinematics of the amputee racer in the turn in which he performed with the inside edge of the ski were similar to those of the outside leg of the non-disabled racer over a turn. In contrast, at the turn in which the amputee racer performed with the outside edge (more difficult side), the amputee racer largely activated the biceps femoris (BF) in the first half of the turn compared to the non-disabled racer. The reason could be to control the angular momentum of the trunk during the forward tilting motion. This is because a greater activity of the BF was observed during the period in which the forward tilt of the trunk was increased, and the mean activity of the BF was the greatest during the first half of the right turn in which the range of the motion of the forward tilt was the greatest. In terms of muscle morphology of the amputee racer, a significant hypertrophy of the BF and vastus lateralis was observed compared to the non-disabled racers. The well-developed BF was considered to be related to the large activity during the turn performed with the outside edge of the ski.

Prediction and classification in equation-free collective motion dynamics.

Modeling the complex collective behavior is a challenging issue in several material and life sciences. The collective motion has been usually modeled by simple interaction rules and explained by global statistics. However, it remains difficult to bridge the gap between the dynamic properties of the complex interaction and the emerging group-level functions. Here we introduce decomposition methods to directly extract and classify the latent global dynamics of nonlinear dynamical systems in an equation-free manner, even including complex interaction in few data dimensions. We first verified that the basic decomposition method can extract and discriminate the dynamics of a well-known rule-based fish-schooling (or bird-flocking) model. The method extracted different temporal frequency modes with spatial interaction coherence among three distinct emergent motions, whereas these wave properties in multiple spatiotemporal scales showed similar dispersion relations. Second, we extended the basic method to map high-dimensional feature space for application to actual small-dimensional systems complexly changing the interaction rules. Using group sports human data, we classified the dynamics and predicted the group objective achievement. Our methods have a potential for classifying collective motions in various domains which obey in non-trivial dominance law known as active matters.

Ultrasound-dependent cytoplasmic internalization of a peptide-sonosensitizer conjugate.

A method to induce cytoplasmic peptide delivery, using ultrasound, was demonstrated using a molecular conjugate of a cell-penetrating peptide (CPP), a functional peptide, and a sonosensitizer. As a model of such molecular conjugates, TatBim-RB, consisting of the Tat CPP, the Bim apoptosis inducing peptide, and the sonosensitizer rose bengal was synthesized. CPPs have been widely used for intracellular delivery of various cargos; however, CPP-fused molecules tend to become entrapped in endosomes, as was observed for TatBim-RB molecules applied to cells. To promote escape of the entrapped TatBim-RB molecules, cells were irradiated with ultrasound, which successfully induced endosomal escape and cytoplasmic dispersion of TatBim-RB, and subsequently apoptosis. Our results suggest that this peptide-sonosensitizer conjugate strategy may facilitate numerous kinds of medicinal chemistry studies, and furthermore, this specific conjugate may exhibit potential as a novel therapeutic agent for the promotion of apoptosis.

Stage IV gastric adenocarcinoma with enteroblastic differentiation with 5-year relapse-free survival after D2 gastrectomy and chemotherapy: A case report.

BACKGROUND: Gastric adenocarcinoma with enteroblastic differentiation (GACED), a rare subtype of gastric cancer, is associated with a more aggressive behavior than conventional gastric adenocarcinomas. We report a rare case of stage IV GACED treated with D2 gastrectomy and postoperative chemotherapy. CASE PRESENTATION: A 39-year-old woman with acute upper abdominal pain immediately underwent surgery for gastric perforation. Afterward she was diagnosed with adenocarcinoma of the pylorus. D2 gastrectomy was performed and the final pathological diagnosis was stage IV GACED with positive peritoneal cytology. Postoperative chemotherapy was initiated with S1 plus oxaliplatin for 1 year, which was ceased thereafter to enhance her quality of life. The patient survived more than 5 years without relapse after gastrectomy. CONCLUSIONS: Stage IV GACED, determined by positive spalt-like transcription factor 4, can be successfully treated with surgery and chemotherapy.

Short-term landing training attenuates landing impact and improves jump height in landing-to-jump movement.

Landing technique is an important factor influencing jump performance in landing-to-jump (L-J) movement. This study examined the effects of short-term landing training on jump performance in L-J. We hypothesized that landing training without jumping decreases landing impact and increases jump height in L-J. Twenty healthy adult men were randomly assigned to the control (CG: n = 10) or the training (TG: n = 10) group. The TG performed a 2-week landing training (3 times per week, 6 sessions) that aims to decrease impact force. Before and after the training period, both groups performed landing and L-J from a 35-cm height and also squat jumps (SJs). Ground reaction forces and kinematic data were obtained during the landing, L-J, and SJ. The CG showed no significant changes in all measured variables. In the TG, the peak vertical ground reaction force up to 100 mseconds after ground contact in L-J, expressed relative to body mass, significantly decreased (pre: 3.04 [0.77] vs. post: 2.35 [0.37], p < 0.01), and the L-J height significantly increased (pre: 47.2 [5.6] cm vs. post: 48.2 [5.5] cm, p < 0.05) without gain in SJ height. Furthermore, the TG showed significant gains (p < 0.01) in hip joint power during the propulsive phase. The current results support our hypothesis and indicate that short-term landing training improves the technique for absorbing landing impact and increasing L-J height. The increased L-J height may be a result of an increase in power generation around the hip joint.

A biomechanical study of side steps at different distances.

Lateral quickness is a crucial component of many sports. However, biomechanical factors that contribute to quickness in lateral movements have not been understood well. Thus, the purpose of this study was to quantify 3-dimensional kinetics of hip, knee, and ankle joints in side steps to understand the function of lower extremity muscle groups. Side steps at nine different distances were performed by nine male subjects. Kinematic and ground reaction force data were recorded, and net joint torque and work were calculated by a standard inverse- dynamics method. Extension torques and work done at hip, knee, and ankle joints contributed substantially to the changes in side step distances. On the other hand, hip abduction work was not as sensitive to the changes in the side step distances. The main roles of hip abduction torque and work were to accelerate the center of mass laterally in the earlier phase of the movement and to keep the trunk upright, but not to generate large power for propulsion.

Biomechanical role can vary depending on the conditions of the motor task.

Expert players in throwing sports may reduce the variability of projectile arrival position by systematically relating release parameters (e.g., release position, velocity, and angular velocity of the projectile). Reducing the variability of the projectile arrival position is often believed to increase the success rate of throwing task, but it may not be always true. Here, we experimentally illustrate that the systematic relationship between release parameters that reduce the variability of the ball arrival position may not increase the number of hitting trials during a throwing task. Furthermore, we demonstrate that the role of the release parameters in increasing successful trials can vary depending on the target size. Each participant threw balls at two different-sized targets (small and large target conditions). Additionally, they alternately threw balls with overhead and sidearm throwing for both the small and large targets. Our results showed that the release position and velocity in the left-right direction reduced the variability of the ball arrival position and increased the successful trials in the small target condition. In the large target condition, the two release parameters reduced the variability of the ball arrival position, but they did not increase the successful trials. Consequently, reducing the variability of the ball arrival position did not always equate to an increase in successful trials, as it depended on the target size. These findings indicate that the role of the release parameters in increasing hitting trials is not constant but varies depending on the condition of the motor task.

Role of the coordinated activities of trunk and lower limb muscles during the landing-to-jump movement.

This study aimed to clarify how the activities of trunk and lower limb muscles during a landing-to-jump (L-J) movement are coordinated to perform the task effectively. Electromyography (EMG) activities of trunk and lower limb muscles as well as kinematic and ground reaction force data were recorded while 17 subjects performed 5 L-Js from a height of 35 cm. The L-J was divided into four phases: PRE phase, 100 ms preceding ground contact; ABSORPTION phase, from ground contact through 100 ms; BRAKING phase, from the end of the ABSORPTION phase to the time of the lowest center of mass position; and PROPULSION phase, from the end of the BRAKING phase to takeoff. The trunk extensor and flexors showed reciprocal activation patterns through the L-J. In the PROPULSION phase, the timings when the EMG activities of the extensor muscles peaked were characterized as a sequential proximal-to-distal pattern. Furthermore, the peak vertical ground reaction force in the ABSORPTION phase relative to body mass negatively correlated to the jump height of the L-J movement and positively correlated with the magnitude of the EMG activities of the soleus in the PRE phase and those of the soleus and rectus abdominis in the ABSORPTION phase. These findings indicate that the intensities and peak timings of muscle activities in the trunk and lower limb are coordinated during the L-J movement and, the coordinated activities would play functional roles such as impact absorption, braking against the descent of body and force generation and direction control for jumping.

Kinetic Analysis of the Fingers Under Different Ball Velocities During Overarm Throwing.

The purpose of this study is to examine changes in the kinetic parameters of the fingers caused by differences in ball velocity during overarm throwing. Six baseball players participated in the study, and the kinetics of the wrist and metacarpophalangeal (MP) joint were calculated using an inverse dynamics method. The results of Tukey's multiple comparison tests showed that the torque and work of the wrist increased with increasing ball velocity (p < .05), indicating that wrist torque and work contributed to the adjustment of ball velocity. Peak MP joint torque also increased with ball velocity (p < .05), although the work of the MP joint remained relatively constant. We conclude that MP joint torque and work contribute to the achievement of stable ball release rather than adjusting ball velocity.

Utilizing hierarchical redundancy for accurate throwing movement.

Understanding how athletes reduce motor variability in redundant tasks contributes to improving sports performance and elucidating human motor control mechanisms. This study aimed to clarify how experienced basketball players use two hierarchical redundancies, ball-level and body-level, for accurate free-throw shooting as no study has simultaneously examined how these hierarchical redundancies are used. Experienced basketball players (intermediate-level and top-level) participated in a free-throw shooting experiment using a motion capture system under two conditions: with feedback (FB) and no-feedback (NF) conditions. To quantify the coordination, the solution manifold and tolerance, noise, and covariation analysis were used for the ball-level redundancy, while uncontrolled manifold analysis and the covariation by randomization method were used for the body-level redundancy. The ball-level analysis revealed that the covariation and noise components were related to the performance, and that the noise component showed a larger contribution to performance than the covariation component, indicating that the reproducibility of the release parameters has a larger room for improvement than coordination. The coordination of release parameters was not significantly different between the FB and NF conditions, indicating that the effect of performance feedback on coordination is unclear. The body-level analysis revealed that interjoint coordination reduced the variability of the longitudinal positions of the hand and fingertip, showing that interjoint coordination improves the reproducibility of the ball-release position, especially in the direction that strongly affects the motor performance. In conclusion, interjoint coordination improved the reproducibility of ball-release parameters, which enhanced motor performance in basketball free-throw shooting.

Kinetic analysis of the wrist and fingers during fastball and curveball pitches.

This study had two objectives: (a) revealing kinetic parameter differences at the fingers during a fastball and curveball, and (b) examining timing control between the wrist and finger torques. The participants were eight baseball pitchers. The kinetics of the wrist and fingers were calculated using an inverse dynamics method. The peak torque and work of finger adduction during the curveball was significantly larger than that during the fastball. During the fastball pitch, the maximal correlation coefficient between the wrist flexion torque and finger flexion torque was very high (r = 0.94 ± 0.05). The reasons for this result are twofold: (a) the extrinsic finger muscles cross the wrist (biarticular muscle) and (b) the wrist flexion torque during the fastball pitch acts in the direction of acceleration of the ball. During the curveball pitch, we found two typical types of wrist and finger torque control. Furthermore, the two pitchers exerted large wrist extension and radial torque, and finger adduction torque. Although the other six pitchers hardly exerted these torques, they exerted wrist flexion torque predominantly. It was considered that the six pitchers selected wrist flexion torque as the control for the fastball and curveball pitch to confuse the batter.

A topspin rate exceeding 110 rps reduces the ball time of arrival to the opponent: a table tennis rally study.

This study experimentally investigated the factors affecting the time a table tennis ball with topspin takes to reach the opponent. Six skilled young players and one coach performed topspin forehand strokes under the observation of three high-speed cameras. As the distribution of the participants' measurements was uneven, additional data were collected using a launching machine that could control the ball speed and spin. To verify the effect of the spin rate on speed decay by drag, the translational speed was measured at 0.15 s after passing the baseline (23 m/s); the balls with topspin ≥110 rps, close to participants' average (117 ± 29 rps) were 1.4 m/s faster than those with topspin ≤80 rps. The horizontal ball speed changed in the range of -3.1 to 2.6 m/s owing to table bounce. At topspins ≥110 rps the ball reached a point 1 m past the end line (estimated receiving position) 27 ± 5 ms faster than at topspins ≤80 rps, for the same initial speed. The relationship between spin rate and travel time was non-linear with boundaries at 80 and 110 rps. Therefore, maintaining a spin rate of ≥ 110 rps along with a high initial speed is an effective strategy for reducing the opponent's preparation time.

Simultaneous monitoring of disease and microbe dynamics through plasma DNA sequencing in pediatric patients with acute lymphoblastic leukemia.

Treatment of acute lymphoblastic leukemia (ALL) necessitates continuous risk assessment of leukemic disease burden and infections that arise in the setting of immunosuppression. This study was performed to assess the feasibility of a hybrid capture next-generation sequencing panel to longitudinally measure molecular leukemic disease clearance and microbial species abundance in 20 pediatric patients with ALL throughout induction chemotherapy. This proof of concept helps establish a technical and conceptual framework that we anticipate will be expanded and applied to additional patients with leukemia, as well as extended to additional cancer types. Molecular monitoring can help accelerate the attainment of insights into the temporal biology of host-microbe-leukemia interactions, including how those changes correlate with and alter anticancer therapy efficacy. We also anticipate that fewer invasive bone marrow examinations will be required, as these methods improve with standardization and are validated for clinical use.

Directional Control Mechanisms in Multidirectional Step Initiating Tasks.

Typical anticipatory postural adjustments (APAs) in forward gait or step initiation tasks to prepare for possible disturbances caused by prime voluntary movements and to accelerate the body forward have been previously reported. However, it is not clear how wide the variations in step directions are differentiated and controlled in non-forward step initiation tasks during the APA phase. The main goal of this study is to explain the directional control mechanisms by investigating the APA of step initiation tasks in forward, diagonal, lateral, and posterior directions. The center of pressure (COP) trajectories and related muscle (soleus, tibialis anterior, and gluteus medius of both lower limbs) activities during the APA of step initiation tasks in nine different directions were analyzed in six healthy young males. Posterior shifts of COP during APA decreased as the direction became more lateral (0° to 90°). For posterior step initiations, COP moved anteriorly from the initial position to accelerate the center of mass of the whole body (COM) backward. Lateral shifts of COP toward the stepping foot during APA decreased as the stepping direction became more lateral (from 0° to 45° and from 180° to 113°) while it plateaued to about zero in the direction from 45° to 113°. Both anteroposterior and lateral displacements of COP in APA were nonlinearly modulated to each direction, but they were linearly related to the anteroposterior and mediolateral component of the velocities of COM at the take-off of the stance foot. Thus, the scaling of APA, reflected in the anteroposterior and lateral displacements of COP and the temporal sequence of selected muscle activities, was based on the anteroposterior and mediolateral components of the take-off velocity of COM that ultimately controls the direction of steps.

Basketball players minimize the effect of motor noise by using near-minimum release speed in free-throw shooting.

The aim of this study was to clarify the strategy used by basketball players during free-throw shooting to improve performance in the presence of motor noise. Two possible hypotheses were examined: the players minimize the release speed to decrease signal-dependent noise or the players maximize the shot success probability by accounting for their variability. Eight collegiate players and one professional player participated in this study by attempting shots from the free-throw line using a motion capture system. The solution manifold consisting of ball parameters at release was calculated and the optimal strategy was simulated by considering ball parameter variability; this result was compared with the actual data. Our results showed that participants selected the solution of near-minimum release speed. The deviation of the measured release angle from the minimum-speed angle was close to zero (2.8 ± 3.1∘). However, an increase in speed-dependent noise did not have a significant influence on the ball landing position through simulation. Additionally, the effect of release angle error on the ball landing position was minimum when using the minimum speed strategy. Therefore, the players minimize the release speed to minimize the effect of the release error on performance, instead of minimizing the speed-dependent noise itself. In other words, the strategy is "near-minimum-speed strategy" as well as "minimum-error-propagation strategy". These findings will be important for understanding how sports experts deal with intrinsic noise to improve performance.

Physically-interpretable classification of biological network dynamics for complex collective motions.

Understanding biological network dynamics is a fundamental issue in various scientific and engineering fields. Network theory is capable of revealing the relationship between elements and their propagation; however, for complex collective motions, the network properties often transiently and complexly change. A fundamental question addressed here pertains to the classification of collective motion network based on physically-interpretable dynamical properties. Here we apply a data-driven spectral analysis called graph dynamic mode decomposition, which obtains the dynamical properties for collective motion classification. Using a ballgame as an example, we classified the strategic collective motions in different global behaviours and discovered that, in addition to the physical properties, the contextual node information was critical for classification. Furthermore, we discovered the label-specific stronger spectra in the relationship among the nearest agents, providing physical and semantic interpretations. Our approach contributes to the understanding of principles of biological complex network dynamics from the perspective of nonlinear dynamical systems.

Evaluation of Plasma Microbial Cell-Free DNA Sequencing to Predict Bloodstream Infection in Pediatric Patients With Relapsed or Refractory Cancer.

Importance: Bloodstream infection (BSI) is a common, life-threatening complication of treatment for cancer. Predicting BSI before onset of clinical symptoms would enable preemptive therapy, but there is no reliable screening test. Objective: To estimate sensitivity and specificity of plasma microbial cell-free DNA sequencing (mcfDNA-seq) for predicting BSI in patients at high risk of life-threatening infection. Design, Setting, and Participants: A prospective pilot cohort study of mcfDNA-seq for predicting BSI in pediatric patients (<25 years of age) with relapsed or refractory cancers at St Jude Children's Research Hospital, a specialist quaternary pediatric hematology-oncology referral center. Remnant clinical blood samples were collected during chemotherapy and hematopoietic cell transplantation. Samples collected during the 7 days before and at onset of BSI episodes, along with negative control samples from study participants, underwent blinded testing using a mcfDNA-seq test in a Clinical Laboratory Improvement Amendments/College of American Pathologists-approved laboratory. Main Outcomes and Measures: The primary outcomes were sensitivity of mcfDNA-seq for detecting a BSI pathogen during the 3 days before BSI onset and specificity of mcfDNA-seq in the absence of fever or infection in the preceding or subsequent 7 days. Results: Between August 9, 2017, and June 4, 2018, 47 participants (27 [57%] male; median age [IQR], 10 [5-14] years) were enrolled; 19 BSI episodes occurred in 12 participants, and predictive samples were available for 16 episodes, including 15 bacterial BSI episodes. In the 3 days before the onset of infection, predictive sensitivity of mcfDNA-seq was 75% for all BSIs (12 of 16; 95% CI, 51%-90%) and 80% (12 of 15; 95% CI, 55%-93%) for bacterial BSIs. The specificity of mcfDNA-seq, evaluated on 33 negative control samples from enrolled participants, was 82% (27 of 33; 95% CI, 66%-91%) for any bacterial or fungal organism and 91% (30 of 33; 95% CI, 76%-97%) for any common BSI pathogen, and the concentration of pathogen DNA was lower in control than predictive samples. Conclusions and Relevance: A clinically relevant pathogen can be identified by mcfDNA-seq days before the onset of BSI in a majority of episodes, potentially enabling preemptive treatment. Clinical application appears feasible pending further study. Trial Registration: ClinicalTrials.gov identifier: NCT03226158.

Kinetic Analysis of Fingers During Aimed Throwing.

This study had two objectives: (a) revealing the difference in finger segments between the conventional and finger models during aimed throwing and (b) examining the central nervous system's timing control between the wrist torque and finger torque. Participants were seven baseball players. Finger kinetics was calculated by an inverse dynamics method. In the conventional model, wrist flexion torque was smaller than that in the finger model because of the error in ball position approximation. The maximal correlation coefficient between the wrist torque and finger torque was high (r = .85 ± .10), and the time lag at maximal correlation coefficient was small (t = 0.36 ± 3.02 ms). The small timing delay between the wrist torque and finger torque greatly influenced ball trajectory. We conclude that, to stabilize release timing, the central nervous system synchronized the wrist torque and finger torque by feed-forward adjustments.

Automatically recognizing strategic cooperative behaviors in various situations of a team sport.

Understanding multi-agent cooperative behavior is challenging in various scientific and engineering domains. In some cases, such as team sports, many cooperative behaviors can be visually categorized and labeled manually by experts. However, these actions which are manually categorized with the same label based on its function have low spatiotemporal similarity. In other words, it is difficult to find similar and different structures of the motions with the same and different labels, respectively. Here, we propose an automatic recognition system for strategic cooperative plays, which are the minimal, basic, and diverse plays in a ball game. Using player's moving distance, geometric information, and distances among players, the proposed method accurately discriminated not only the cooperative plays in a primary area, i.e., near the ball, but also those distant from a primary area. We also propose a method to classify more detailed types of cooperative plays in various situations. The proposed framework, which sheds light on inconspicuous players to play important roles, could have a potential to detect well-defined and labeled cooperative behaviors.