RESUMEN
The transverse acoustic impedance of superfluid ^{3}He was measured in the A1 and A2 phases up to 13 T to investigate the surface states in nonunitary superfluids. The temperature dependence of the impedance was much larger in the A1 phase than in the A2 phase. This nonsymmetric behavior indicates that momentum exchange with walls for spin-down surface states is quite different from that for spin-up surface states. The spin-dependent response might be a reflection of an essential feature of the nonunitary states where gap amplitudes depend on spin states. Weak-coupling theories ignore any spin-dependent processes and do not account for the nonsymmetric behavior.
RESUMEN
The magnetic properties of (3)He in its various phases originate from the interactions among the nuclear spins. The spin-polarized 'ferromagnetic' superfluid (3)He A(1) phase (which forms below 3 mK between two transition temperatures, T(c1) and T(c2), in an external magnetic field) serves as a material in which theories of fundamental magnetic processes and macroscopic quantum spin phenomena may be tested. Conventionally, the superfluid component of the A(1) phase is understood to contain only the majority spin condensate, having energetically favoured paired spins directed along the external field and no minority spin condensate having paired spins in the opposite direction. Because of difficulties in satisfying both the ultralow temperature and high magnetic field required to produce a substantial phase space, there exist few studies of spin dynamics phenomena that could be used to test the conventional view of the A(1) phase. Here we develop a mechanical spin density detector that operates in the required regime, enabling us to perform measurements of spin relaxation in the A(1) phase as a function of temperature, pressure and magnetic field. Our mechanical spin detector is based in principle on the magnetic fountain effect; spin-polarized superfluid motion can be induced both magnetically and mechanically, and we demonstrate the feasibility of increasing spin polarization by a mechanical spin filtering process. In the high temperature range of the A(1) phase near T(c1), the measured spin relaxation time is long, as expected. Unexpectedly, the spin relaxation rate increases rapidly as the temperature is decreased towards T(c2). Our measurements, together with Leggett-Takagi theory, demonstrate that a minute presence of minority spin pairs is responsible for this unexpected spin relaxation behaviour. Thus, the long-held conventional view that the A(1) phase contains only the majority spin condensate is inadequate.
RESUMEN
The molecular nature of sweet taste receptors has not been fully explored. Employing a differential screening strategy, we identified a taste receptor gene, Tre1, that controls the taste sensitivity to trehalose in Drosophila melanogaster. The Tre1 gene encodes a novel protein with similarity to G protein-coupled seven-transmembrane receptors. Disruption of the Tre1 gene lowered the taste sensitivity to trehalose, whereas sensitivities to other sugars were unaltered. Overexpression of the Tre1 gene restored the taste sensitivity to trehalose in the Tre1 deletion mutant. The Tre1 gene is expressed in taste sensory cells. These results provide direct evidence that Tre1 encodes a putative taste receptor for trehalose in Drosophila.
Asunto(s)
Proteínas de Drosophila , Drosophila melanogaster/genética , Genes de Insecto , Receptores de Superficie Celular/genética , Receptores Acoplados a Proteínas G , Gusto , Trehalosa , Animales , Animales Modificados Genéticamente , Southern Blotting , Clonación Molecular , ADN Complementario , Drosophila melanogaster/química , Femenino , Hibridación Fluorescente in Situ , Masculino , Datos de Secuencia Molecular , Mutación , Estructura Terciaria de Proteína , Receptores de Superficie Celular/química , Receptores de Superficie Celular/metabolismoRESUMEN
BACKGROUND: Impaired production/release of defensins, representative endogenous antimicrobial peptides, is associated with the pathogenesis of inflammatory bowel disease (IBD). MATERIAL AND METHODS: Employing in house radioimmunoassay, we examined concentrations of the major forms alpha-defensins, human neutrophil peptides (HNP) 1-3 and human beta-defensin (HBD)-2 in plasma of 55 IBD patients consisting of 29 patients with ulcerative colitis (UC) and 26 with Crohn's disease (CD) and 57 controls. RESULTS: The circulating HNP 1-3, but not HBD-2, levels in IBD patients were significantly higher than those in controls. Plasma HNP 1-3 concentrations in CD patients significantly correlated with Crohn's disease activity index, peripheral white blood cell counts, serum CRP values and TNF-alpha levels. CONCLUSIONS: Elevation of circulating alpha-defensins levels is suggestive of their physiopathological roles in IBD. Plasma HNP 1-3 concentrations may be an indicator for CD activity and their association with CRP and TNF-alpha supports a possible association with the inflammatory process.
Asunto(s)
Enfermedades Inflamatorias del Intestino/sangre , alfa-Defensinas/sangre , beta-Defensinas/sangre , Adolescente , Adulto , Anciano , Antiinfecciosos/sangre , Femenino , Humanos , Enfermedades Inflamatorias del Intestino/patología , Enfermedades Inflamatorias del Intestino/fisiopatología , Masculino , Persona de Mediana Edad , Índice de Severidad de la Enfermedad , Estadística como Asunto , Adulto JovenRESUMEN
OBJECTIVE: To determine the association of distinct clinical subsets with myositis-specific autoantibodies (MSAs) towards anti-155/140-kDa polypeptides [anti-155/140 antibodies (Abs)], anti-140-kDa polypeptides (anti-140 Abs), and anti-aminoacyl tRNA synthetases (ARS Abs) in Japanese patients with dermatomyositis (DM). METHODS: We compared the clinical features and short-term prognoses of 30 DM patients whose serological status included these MSAs. The MSAs were determined by immunoprecipitation. RESULTS: Anti-155/140 Abs (n = 5), anti-140 Abs (n = 8), and anti-ARS Abs (n = 7) did not overlap each other. All of the anti-155/140 Ab-positive patients (n = 5) were complicated by malignancies, as were all of the anti-140 Ab-positive patients (n = 8), who showed rapidly progressive interstitial lung disease (ILD). The survival rate at 6 months from the diagnosis of DM was significantly lower in the anti-140 Ab-positive patients than in the other patients. CONCLUSION: This is the first study to report, in a single cohort of DM patients, that distinct clinical subsets are distributed in an anti-155/140 Ab-positive group, an anti-140 Ab-positive group, or an anti-ARS Ab-positive group. Our data also confirm previous evidence that anti-155/140 Abs are involved in malignancies and that anti-140 Abs are involved in rapidly progressive ILD.
Asunto(s)
Aminoacil-ARNt Sintetasas/inmunología , Autoanticuerpos/inmunología , Dermatomiositis/diagnóstico , Dermatomiositis/inmunología , ARN de Transferencia/inmunología , Adulto , Anciano , Estudios de Cohortes , Estudios Transversales , Femenino , Humanos , Inmunoprecipitación , Japón , Estimación de Kaplan-Meier , Masculino , Persona de Mediana Edad , Péptidos/inmunología , Probabilidad , Estadísticas no ParamétricasRESUMEN
The effects of mastoparan on phosphoinositide hydrolysis and prostaglandin E2 (PGE2) generation were investigated in astrocytes cultured from rabbit brain. Mastoparan inhibited the accumulations of [3H]inositol phosphates induced by bradykinin (1 microM) in a time- and concentration-dependent manner. Mastoparan (3-30 microM) also released PGE2 in a time- and concentration-dependent manner. Mastoparan-induced release of PGE2 was inhibited by indomethacin, a cyclooxygenase inhibitor, by dexamethasone, a steroidal anti-inflammatory drug, and by pertussis toxin, an inactivator of some G proteins, such as Gi and Go. Mastoparan also caused [3H]arachidonic acid liberation, which was inhibited by dexamethasone or pertussis toxin. In contrast, indomethacin, dexamethasone and pertussis toxin failed to attenuate mastoparan-induced inhibition of [3H]inositol phosphate accumulation induced by bradykinin. Thus, mastoparan-induced inhibition of phosphoinositide hydrolysis does not involve pertussis toxin-sensitive G protein nor arachidonic acid metabolites. In addition to the inhibition of phospholipase C, mastoparan activates phospholipase A2 through pertussis toxin-sensitive G protein.
Asunto(s)
Astrocitos/efectos de los fármacos , Dinoprostona/biosíntesis , Fosfatos de Inositol/metabolismo , Venenos de Avispas/farmacología , Animales , Ácido Araquidónico/análisis , Astrocitos/metabolismo , Células Cultivadas , Dexametasona/farmacología , Indometacina/farmacología , Péptidos y Proteínas de Señalización Intercelular , Péptidos , Toxina del Pertussis , Fosfatidilinositoles/metabolismo , Fosfolipasas A/metabolismo , Fosfolipasas A2 , Conejos , Transducción de Señal , Fosfolipasas de Tipo C/antagonistas & inhibidores , Factores de Virulencia de Bordetella/farmacología , Venenos de Avispas/antagonistas & inhibidoresRESUMEN
1. The effect of mastoparan, a wasp venom toxin, on intracellular free Ca2+ concentration ([Ca2+]i) was examined in human astrocytoma cells. Mastoparan inhibited [Ca2+]i induced by carbachol (100 microM) in a concentration-dependent manner in the absence of extracellular Ca2+, consistent with our previous results showing that mastoparan inhibits phosphoinositide hydrolysis in human astrocytoma cells. 2. In contrast, mastoparan itself increased [Ca2+]i and augmented carbachol-induced increase in the [Ca2+]i in the presence of extracellular Ca2+, suggesting that mastoparan elicited Ca2+ influx from the extracellular medium. The increase appeared to be maximum at extracellular Ca2+ concentrations of 0.1-0.2 mM. The higher concentrations of extracellular Ca2+ depressed the influx. 3. Pertussis toxin did not affect mastoparan-induced inhibition of [Ca2+]i in the absence of extracellular Ca2+, consistent with the previous results that pertussis toxin did not affect mastoparan-induced inhibition of phosphoinositide hydrolysis. 4. Pertussis toxin augmented mastoparan-induced increase in [Ca2+]i in the presence of extracellular Ca2+, suggesting that pertussis toxin substrate(s) seems to be inhibitory for Ca2+ influx induced by mastoparan. 5. Verapamil, nifedipine and diltiazem (each 10 microM), L-type Ca2+ antagonists, did not affect mastoparan-induced Ca2+ influx. However, verapamil (10 microM) slightly inhibited the increase in [Ca2+]i induced by carbachol in the presence of mastoparan. 6. The results obtained in the present study indicate that mastoparan has two opposite effects on [Ca2+]i in human astrocytoma cells and possibly has at least two sites of action.
Asunto(s)
Astrocitoma/metabolismo , Calcio/metabolismo , Neoplasias del Sistema Nervioso/metabolismo , Venenos de Avispas/farmacología , Agonistas de los Canales de Calcio/farmacología , Bloqueadores de los Canales de Calcio/farmacología , Carbacol/antagonistas & inhibidores , Carbacol/farmacología , Fura-2 , Proteínas de Unión al GTP/antagonistas & inhibidores , Proteínas de Unión al GTP/fisiología , Humanos , Péptidos y Proteínas de Señalización Intercelular , Péptidos , Toxina del Pertussis , Fosfatidilinositoles/metabolismo , Transducción de Señal/efectos de los fármacos , Células Tumorales Cultivadas , Factores de Virulencia de Bordetella/farmacologíaRESUMEN
NMR studies of submonolayer 3He adsorbed on a bilayer of HD preplated graphite have been made down to 100 &mgr;K, which is more than 1 order of magnitude smaller than the exchange energy ( J). In the highly frustrated antiferromagnetic solid region, the magnetization obeys a Curie-Weiss law even at temperatures around J and then increases gradually down to 100 &mgr;K. Nevertheless, it does not show any anomalous behavior corresponding to a spin gap. The normalized magnetization versus the reduced temperature ( T/J) is independent of the density just after solidification. This is consistent with the result in the high-temperature region, that the main multiple-spin exchanges have a similar density dependence.
RESUMEN
OBJECTIVE: To determine the relationship between maternal serum hCG levels and small for gestational age (SGA) infants. METHODS: Maternal serum hCG levels were measured in 638 consecutive pregnant women in whom gestation was dated by crown-rump length and who later delivered a singleton infant. Fetal growth was compared between the women with elevated maternal hCG (above 2 multiples of the median) and those with normal levels. RESULTS: Elevated maternal hCG values were found in 6.6%, of whom 19% delivered SGA infants. In the normal maternal hCG group, 3.9% of the infants were SGA. These differences were statistically significant by chi 2 analysis. Mothers with elevated hCG also had a significantly higher risk for fetal death, preterm rupture of the membranes, and abruptio placentae by the Fisher exact probability test. CONCLUSION: Elevated maternal serum hCG levels correlate with SGA infants.
Asunto(s)
Gonadotropina Coriónica/sangre , Desarrollo Embrionario y Fetal/fisiología , Recién Nacido Pequeño para la Edad Gestacional , Complicaciones del Embarazo/epidemiología , Resultado del Embarazo/epidemiología , Adulto , Femenino , Humanos , Recién Nacido , Valor Predictivo de las Pruebas , Embarazo , Complicaciones del Embarazo/sangre , Curva ROC , Factores de RiesgoRESUMEN
We have previously shown that human astrocytoma cells (1321N1) express thromboxane A2 (TXA2) receptors, of which stimulation activates phosphoinositide hydrolysis (Nakahata et al., Eur. J. Pharmacol. 162 (1989) 407). In order to examine whether TXA2 receptors exist in native astrocytes or not, rabbit cultured astrocytes were used. Glial fibrillary acidic protein (GFAP)-positive astrocytes were obtained three weeks after culture of brain. [3H]ONO NT-126, a TXA2 antagonist, bound to the membranes derived from cultured rabbit astrocytes with the dissociation constant (Kd) of 0.23 nM and the maximum binding site (Bmax) of 69.5 fmol/mg protein. STA2, a stable TXA2 receptor agonist, activates phosphoinositide hydrolysis in a concentration-dependent manner, and S-145, a TXA2 antagonist, inhibited STA2-induced phosphoinositide hydrolysis. The results indicate that TXA2 receptors exist in cultured rabbit astrocytes and the activation of TXA2 receptors results in phosphoinositide hydrolysis.
Asunto(s)
Astrocitos/metabolismo , Corteza Cerebral/metabolismo , Receptores de Prostaglandina/metabolismo , Tromboxano A2/metabolismo , Animales , Astrocitos/citología , Compuestos Bicíclicos con Puentes/metabolismo , Compuestos Bicíclicos con Puentes/farmacología , Membrana Celular/metabolismo , Células Cultivadas , Ácidos Grasos Monoinsaturados/metabolismo , Ácidos Grasos Monoinsaturados/farmacología , Femenino , Inmunohistoquímica , Cinética , Masculino , Fosfatidilinositoles/metabolismo , Conejos , Receptores de Prostaglandina/análisis , Receptores de Prostaglandina/antagonistas & inhibidores , Receptores de Tromboxanos , Tromboxano A2/análogos & derivados , Tromboxano A2/antagonistas & inhibidores , Tromboxano A2/farmacologíaRESUMEN
In human astrocytoma cells (1321N1), trifluoperazine potently inhibited histamine-induced phosphoinositide hydrolysis, while it slightly inhibited carbachol-induced phosphoinositide hydrolysis. Trifluoperazine as well as diphenhydramine inhibited [3H]mepyramine binding in astrocytoma cell membranes with Ki values of 0.052 microM and 0.005 microM, respectively. However, trifluoperazine only slightly inhibited [3H]quinuclidinyl benzilate binding with a Ki value of 3 microM. These results indicate that trifluoperazine specifically inhibits histamine-induced phosphoinositide hydrolysis as a histamine H1-receptor antagonist in human astrocytoma cells.
Asunto(s)
Antagonistas de los Receptores Histamínicos H1/farmacología , Fosfatidilinositoles/metabolismo , Trifluoperazina/farmacología , Astrocitoma/metabolismo , Carbacol/farmacología , Activación Enzimática , Histamina/farmacología , Humanos , Hidrólisis/efectos de los fármacos , Cinética , Receptores Muscarínicos/efectos de los fármacos , Receptores Muscarínicos/fisiología , Células Tumorales Cultivadas , Fosfolipasas de Tipo C/efectos de los fármacos , Fosfolipasas de Tipo C/metabolismoRESUMEN
Extracellular ATP secreted from stimulated nerves plays a role in neurotransmission. This study examined the effects of extracellular ATP on phospholipase A2 and C signalling pathways in rabbit astrocytes. ATP caused prostaglandin E2 (PGE2) generation and phosphoinositide hydrolysis in a time- and concentration-dependent manner. A P2y purinoceptor-selective agonist, 2-methylthio-ATP also caused phosphoinositide hydrolysis, but not PGE2 generation. A P2x purinoceptor-selective agonist, alpha, beta-methylene-ATP did not cause either phosphoinositide hydrolysis or PGE2 generation. Although pertussis toxin had no effect on 2-methylthio-ATP-induced phosphoinositide hydrolysis, it markedly decreased ATP-induced PGE2 generation, with significant inhibition of phosphoinositide hydrolysis. Dexamethasone and indomethacin which potently inhibited ATP-induced PGE2 generation, caused partial inhibition of phosphoinositide hydrolysis, suggesting that pertussis toxin-sensitive component of ATP-induced phospholipase C activation is mediated by cyclo-oxygenase metabolites of arachidonic acid. These results suggest that a stimulation of P2y receptor results in phospholipase C activation in a pertussis toxin-insensitive manner, and that a P2 receptor other than the P2y or P2x subtypes is involved in ATP-induced phospholipase A2 activation via a pertussis toxin-sensitive G protein.