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OBJECTIVE: This research was designed to probe into the expression and related mechanism of lncRNA NR2F1-AS1 and H19 in hepatocellular carcinoma (HCC). METHODS: Forty-two HCC patients who came to our hospital from February 2018 to August 2019 were included into a research group (RG). Meanwhile, 46 healthy controls were regarded as a control group (CG). BEL-7402, Huh-7 human hepatoma cells and HL-7702 human normal liver cells were purchased, and the NR2F1-AS1 and H19 levels in serum and tissues of HCC patients were detected. PcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19 and si-H19 were transfected into BEL-7402 and Huh-7 cells. The NR2F1-AS1 and H19 levels in samples were detected via qRT-PCR, and the expression of apoptosis-related proteins in cells was tested through WB. Cell proliferation, invasion, or apoptosis was detected by CCK8, Transwell or flow cytometry, respectively. RESULTS: The NR2F1-AS1 and H19 levels were high in human hepatoma cells, and AUCs of lncRNA NR2F1-AS1 and lncRNA H19 were both >0.8. The lncRNA NR2F1-AS1 and lncRNA H19 were associated with HCC staging. After transfection of pcDNA3.1-NR2F1-AS1, si-NR2F1-AS1, NC, pcDNA3.1-H19, si-H19 BEL-7402 and Huh-7 cells, silencing NR2F1-AS1 and H19 expression can promote apoptosis and inhibit cell growth, while silencing their over-expression can inhibit the EMT process of Huh-7 cells. CONCLUSION: lncRNA NR2F1-AS1 and lncRNA H19 genes are abnormally expressed in HCC. Furthermore, the two can suppress the EMT process of Huh-7 cells and promote apoptosis effectively.
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Current sources with extremely low noise are significant for many branches of scientific research, such as experiments of ultra-cold atoms, superconducting quantum computing, and precision measurements. Here we construct and characterize an analog-controlled bipolar current source with high bandwidth and ultra-low noise. A precise and stable resistor is connected in series with the output for current sensing. After being amplified with an instrumentation amplifier, the current sensing signal is compared with an ultra-low noise reference, and proportional-integral (PI) calculations are performed via a zero-drift low-noise operational amplifier. The result of the PI calculation is sent to the output power operational amplifier for closed-loop control of the output current. In this way, a current of up to 16 A can be sourced to or sunk from a load with a compliance voltage of greater than ±12 V. The broadband current noise of our bipolar current source is about 0.5 µA/Hz and 1/f corner frequency is less than 1 Hz. Applications of this current source in a cold atom interferometer, as well as active compensation of a stray magnetic field, are presented. A method for measuring high-frequency current noise in a 10 A DC current with a sensitivity down to a level of 10 µA is also described.