A colloidal gold immunoassay strip assay for cadmium detection in oilfield chemicals.

Cadmium ions (Cd2+) are some of the major pollutants in oilfield chemicals. To reduce the pollution of oilfield chemicals, it is necessary to detect and control the content of Cd2+. In this study, we synthesized a highly sensitive and specific monoclonal antibody against Cd2+ with an IC50 of 1.97 ng mL-1 and no cross-reactivity. Based on this antibody, a colloidal gold immunoassay strip detection assay with an IC50 of 1 mg kg-1 and a detection range of 1.0-20 mg kg-1 in oilfield chemicals was developed. This assay could be completed in 20 min and can be used for Cd2+ on-site testing in oilfield chemicals and improve supervision efficiency in oil exploration and development.

A New Strategy of Chemical Photo Grafting Metal Organic Framework to Construct NH2-UiO-66/BiOBr/PVDF Photocatalytic Membrane for Synergistic Separation and Self-Cleaning Dyes.

Photocatalytic membranes are typical multifunctional membranes that have emerged in recent years. The lack of active functional groups on the surface of membranes made of inert materials such as polyvinylidene fluoride(PVDF) makes it difficult to have a stable binding interaction with photocatalysts directly. Therefore, in this study, we developed a simple method to prepare NH2-UiO-66/BiOBr/PVDF(MUB) membranes for efficient dye treatment by grafting benzophenolic acid-functionalized NH2-UiO-66 onto the surface of membranes with photocatalytic properties under visible light irradiation using benzophenolic acid with photoinitiating ability as an anchor. The structural characteristics, photocatalytic properties, antifouling properties, and reusability of the composite membranes were investigated in subsequent experiments using a series of experiments and characterizations. The results showed that the benzophenone acid grafting method was stable and the nanoparticles were not easily dislodged. The MUB composite membrane achieved a higher dye degradation efficiency (99.2%) than the pristine PVDF membrane at 62.9% within a reaction time of 180 min. In addition, the composite membranes exhibited higher permeate fluxes for both pure and mixed dyes and also demonstrated outstanding water flux recovery (>96%) after the light self-cleaning cycle operation. This combination proved to improve the performance of the membranes instead of reducing them, increasing their durability and reusability, and helping to broaden the application areas of membrane filtration technology.

Quantitative immunochromatographic assay for rapid and cost-effective on-site detection of benzo[a]pyrene in oilfield chemicals.

Contamination of oilfield chemicals (OFCs) by benzo[a]pyrene (B[a]P) is increasingly becoming a severe environmental security issue. There is an urgent need to develop a rapid and accurate method for B[a]P detection in OFCs. In this study, B[a]P hapten was designed using computer aided molecular design. A high-affinity, specific, and matrix-insensitive monoclonal antibody (mAb) with IC50 values of 6.77 ng/mL was obtained. Based on this mAb, we developed a rapid gold nanoparticle-based immunochromatographic strip assay (GICA) with double T-line mode for on-site detection of B[a]P in OFCs samples. The GICA exhibited excellent detection performance in OFCs samples with strong acidity, strong alkalinity, and deep color. Under optimal conditions, the proposed method detected B[a]P in OFCs at 0.42-300 mg/kg, and limit of detection was 0.23-1.07 mg/kg. The recovery rate was 88-106% with a coefficient of variation of 1.46-6.35%. Confirmed by natural positive OFCs samples and high-performance liquid chromatography, this GICA is accurate and reliable, with great potential for rapid and cost-effective on-site detection.

Laboratory Study on the Oil Displacement Process in Low-Permeability Cores with Different Injection Fluids.

Although flooding technology has found wide application in low-permeability reservoir development practices, the oil recovery enhancement mechanisms for different injection fluids still lack specific focus based on comprehensive investigations. Therefore, in this paper, supercritical CO2 (ScCO2), N2, and water injection processes in oil-saturated low-permeability tight cores were comparatively studied. To reveal the effect of physicochemical properties of the injection fluid on the oil recovery efficiency, the Berea sandstones with three permeability levels and kerosene were employed in this study to exclude other parameter influences. The flooding processes employing various injection media were investigated based on quantitative comparisons of the oil recovery factor and the displacement pressure difference at two system pressures. The experimental results show recovery efficiencies of 59-91 and 84-92% with the increasing permeability for the ScCO2 injection process at system pressures of 15 and 25 MPa, respectively, which are much higher than 26-40 and 21-52% in the N2 case and 43-46 and 45-49% in the water cases. Interfacial tension (IFT) measurement results indicate that miscibility conditions have been achieved for the ScCO2/oil system, thus leading to much higher oil recovery. On the other hand, the pressure difference results show a similar magnitude of 10 MPa/m for both ScCO2 and N2 processes, which is much lower than the 100 MPa/m for the water flooding cases. Comprehensive comparison shows that ScCO2 shows great advantages in the application of unconventional reservoirs. It is expected that our research work could enrich the investigations of CO2 flooding and the in-depth understanding of the mechanisms and better guide the utilization of CO2.