RESUMEN
Human lymphatic filariasis is a neglected tropical disease, causing permanent and long-term disability with severe immunopathology. Abundant larval transcript (ALT) plays a crucial role in parasite establishment in the host, due to its multi-faceted ability in host immune regulation. Although ALT protein is a key filarial target, its exact function is yet to be explored. Here, we report epitope mapping and a structural model of Brugia malayi ALT-2, leading to development of a multi-epitope vaccine. Structural analysis revealed that ALT represents unique parasitic defence proteins belonging to a toxin family that carries a 'knottin' fold. ALT-2 has been a favourite vaccine antigen and was protective in filarial models. Due to the immunological significance of ALT-2, we mapped B-cell epitopes systematically and identified two epitope clusters, 1-30 and 89-128. To explore the prophylactic potential of epitope clusters, a recombinant multi-epitopic gene comprising the epitopic domains was engineered and the protective efficacy of recombinant ALT epitope protein (AEP) was tested in the permissive model, Mastomys coucha. AEP elicited potent antibody responses with predominant IgG1 isotype and conferred significantly high protection (74.59%) compared to ALT-2 (61.95%). This proved that these epitopic domains are responsible for the protective efficacy of ALT-2 and engineering protective epitopes as a multi-epitope protein may be a novel vaccine strategy for complex parasitic infections.
Asunto(s)
Antígenos Helmínticos/inmunología , Filariasis Linfática/prevención & control , Mapeo Epitopo , Epítopos de Linfocito B/inmunología , Proteínas Recombinantes/inmunología , Vacunas Sintéticas/inmunología , Animales , Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/genética , Modelos Animales de Enfermedad , Filariasis Linfática/inmunología , Epítopos de Linfocito B/genética , Inmunoglobulina G/sangre , Murinae , Proteínas Recombinantes/genética , Resultado del Tratamiento , Vacunas Sintéticas/administración & dosificación , Vacunas Sintéticas/genéticaRESUMEN
Global programmes to eliminate lymphatic filariasis (GPELF) require mapping, monitoring and evaluation using filarial antigen diagnostic kits. To meet this objective, a functional single-chain fragment variable (ScFv) specific for filarial Wuchereria bancrofti SXP-1 (Wb-SXP-1) antigen was constructed for the diagnosis of active filarial infection, an alternative to the production of complete antibodies using hybridomas. The variable heavy chain (VH) and the variable light chain (kappa) (Vκ) genes were amplified from the mouse hybridoma cell line and were linked together with a flexible linker by overlap extension polymerase chain reaction (PCR). The ScFv construct (Vκ-Linker-VH) was expressed as a fusion protein with N-terminal His tag in Escherichia coli and purified using immobilized metal affinity chromatography (IMAC) without the addition of reducing agents. Immunoblotting and sandwich enzyme-linked immunosorbent assay (ELISA) were used to analyse the antigen binding affinity of purified ScFv. The purified ScFv was found to recognize recombinant and native Wb-SXP-1 antigen in microfilariae (Mf)-positive patient sera. The affinity of ScFv was comparable with that of the monoclonal antibody. The development of recombinant ScFv to replace monoclonal antibody for detection of filarial antigen was achieved. The recombinant ScFv was purified, on-column refolded and its detection ability validated using field samples.
Asunto(s)
Antígenos Helmínticos/sangre , Filariasis Linfática/diagnóstico , Anticuerpos de Cadena Única/análisis , Wuchereria bancrofti/inmunología , Animales , Antígenos Helmínticos/genética , Antígenos Helmínticos/inmunología , Filariasis Linfática/sangre , Filariasis Linfática/parasitología , Ensayo de Inmunoadsorción Enzimática , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Humanos , Ratones , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Anticuerpos de Cadena Única/genética , Anticuerpos de Cadena Única/inmunología , Wuchereria bancrofti/genética , Wuchereria bancrofti/aislamiento & purificaciónRESUMEN
Lymphatic filariasis, a mosquito-borne parasitic disease, affects more than 120 million people worldwide. Vaccination for filariasis by targeting different stages of the parasite will be a boon to the existing MDA efforts of WHO which required repeated administration of the drug to reduce the infection level and sustained transmission. Onset of a filaria-specific immune response achieved through antigen vaccines can act synergistically with these drugs to enhance the parasite killing. Multi-epitope vaccine approach has been proved to be successful against several parasitic diseases as it overcomes the limitations associated with the whole antigen vaccines. Earlier results from our group suggested the protective efficacy of multi-epitope vaccine comprising two immunodominant epitopes from Brugia malayi antioxidant thioredoxin (TRX), several epitopes from transglutaminase (TGA) and abundant larval transcript-2 (ALT-2). In this study, the prophylactic efficacy of the filarial epitope protein (FEP), a chimera of selective epitopes identified from our earlier study, was tested in a murine model (jird) of filariasis with L3 larvae. FEP conferred a significantly (P < 0.0001) high protection (69.5%) over the control in jirds. We also observed that the multi-epitope recombinant construct (FEP) induces multiple types of protective immune responses, thus ensuring the successful elimination of the parasite; this poses FEP as a potential vaccine candidate.
Asunto(s)
Filariasis Linfática/prevención & control , Epítopos Inmunodominantes/administración & dosificación , Vacunas Antiprotozoos/inmunología , Proteínas Recombinantes de Fusión/administración & dosificación , Animales , Anticuerpos Antihelmínticos/inmunología , Anticuerpos Antiprotozoarios/sangre , Antígenos Helmínticos/inmunología , Brugia Malayi/inmunología , Brugia Malayi/patogenicidad , Modelos Animales de Enfermedad , Gerbillinae , Proteínas del Helminto/inmunología , Humanos , Epítopos Inmunodominantes/inmunología , Masculino , Ratones , Vacunas Antiprotozoos/administración & dosificación , Proteínas Recombinantes de Fusión/inmunología , Proteínas Recombinantes/inmunología , Tiorredoxinas/inmunología , Transglutaminasas/inmunología , Vacunación , Wuchereria bancrofti/patogenicidadRESUMEN
Lymphatic filariasis is a parasitic disease of tropical countries. This is a disfiguring and painful disease contracted in childhood, but the symptoms become apparent only in later years. Diagnosis of filarial infection is very crucial for the management of the disease. The main objective of this study was to develop a filarial antigen-based immunological assay for the diagnosis and surveillance of the disease. Monoclonal and polyclonal antibodies were raised to the recombinant protein Brugia malayi vespid allergen homologue (VAH). Capture enzyme-linked immunosorbent assay (ELISA) was standardized utilizing various combinations of antibodies and evaluated with serum samples of endemic normal (EN, n= 110), microfilaraemic (MF, n= 65), chronic pathology (CP, n= 45) and non-endemic normal (NEN, n= 10) individuals. Of the 230 samples tested, VAH capture assay detected circulating antigen in 97.91% of bancroftian and 100% of brugian microfilaraemic individuals, and 5% of endemic normal individuals, comparable to the earlier reported SXP-1 antigen detection assay. However, the combination of VAH and SXP-1 (VS) capture ELISA was found to be more robust, detecting 100% of microfilaraemic individuals and with higher binding values. Thus an antigen capture immunoassay has been developed, which can differentiate active infection from chronic infection by detecting circulating filarial antigens in clinical groups of endemic areas.
Asunto(s)
Antígenos Helmínticos/análisis , Brugia Malayi/aislamiento & purificación , Filariasis Linfática/diagnóstico , Ensayo de Inmunoadsorción Enzimática/métodos , Animales , Sensibilidad y EspecificidadRESUMEN
Filariasis caused by infectious parasitic nematodes has been identified as the second leading source of permanent and long-term disability in Sub-Saharan Africa, Asia and Latin America. Several vaccine candidates were identified from infective third-stage larvae (L3) which involves in the critical transition from arthropod to human. Hitherto studies of these antigens in combination with alum adjuvant have shown to elicit its characteristic Th2 responses. Inulin is a safe, non-toxic adjuvant that principally stimulates the innate immune response through the alternative complement pathway. In the present study, the immune response elicited by inulin and alum as adjuvants were compared with filarial antigens from different aetiological agents: secreted larval acidic protein 1 (SLAP1) from Onchocerca volvulus and venom allergen homologue (VAH) from Brugia malayi as single or as cocktail vaccines in mice model. The study revealed that inulin can induce better humoral response against these antigens than alum adjuvant. Antibody isotyping disclosed inulin's ability to elevate the levels of IgG2a and IgG3 antibodies which mediates in complement-dependent cytotoxicity and antibody-dependent cell-mediated cytotoxicity (ADCC), respectively, in mice. Splenocyte analysis showed that T cells prestimulated with inulin have higher stimulation index (P < 0.05) than alum except for BmVAH antigen. In vitro ADCC assay showed that inulin formulation had induced higher cytotoxicity with filarial antigens (as single P < 0.01 and as cocktail P < 0.05, respectively) than alum. The results had confirmed the capability of inulin to deplete the levels of Treg and brought a balance in Th1/Th2 arms against filarial antigens in mice.
Asunto(s)
Adyuvantes Inmunológicos , Compuestos de Alumbre/farmacología , Antígenos Helmínticos/inmunología , Filariasis/prevención & control , Inulina/inmunología , Animales , Anticuerpos Antihelmínticos/inmunología , Brugia Malayi/inmunología , Modelos Animales de Enfermedad , Filariasis/inmunología , Proteínas del Helminto/inmunología , Inmunoglobulina G/sangre , Inmunoglobulina G/inmunología , Inulina/farmacología , Masculino , Ratones , Ratones Endogámicos BALB C , Óxido Nítrico Sintasa de Tipo II/biosíntesis , Óxido Nítrico Sintasa de Tipo II/genética , Onchocerca volvulus/inmunología , Vacunas Antiprotozoos , ARN Mensajero/biosíntesis , Linfocitos T Reguladores/inmunología , Células TH1/inmunología , Células Th2/inmunología , VacunaciónRESUMEN
The chromadorea abundant larval transcript (ALT) family of proteins contains ALT one of the most studied putative vaccine candidate in experimental filariasis. This study reports the characterization of Wuchereria bancrofti 20/22 (Wb20/22) as a member of chromadorea, the ALT family of proteins from the L3 stage of W. bancrofti. The high reactivity with serum from the endemic normal (EN) population suggests that Wb20/22 could be a target of elicit protective immunity. The glutamic acid-rich region of Wb20/22 was predicted to harbour the longest linear B-cell epitope by insilico prediction tools. The significance of this region was revealed by studying the mutant form of Wb20/22, without acidic domain (WOAD) which was cloned, and the immune response was compared with Wb20/22. The signal sequence of Wb20/22 was also an immunodominant region, and mutant construct without signal sequence (WOSS) was cloned and characterized. The peak antibody titre elicited by WOAD was higher than Wb20/22 or WOSS, which pointed to the immunomodulatory role of glutamic acid-rich region. Wb20/22 elicited very high levels of IL-10 and diminished levels of IL-4 and IL-5 which could be the reason for low antibody titre. The prophylactic efficacy of WOAD conferred protection (62·26%) which was higher than Wb20/22 (49·82%) and WOSS (54·78%).
Asunto(s)
Antígenos Helmínticos/aislamiento & purificación , Wuchereria bancrofti/genética , Secuencia de Aminoácidos , Animales , Antígenos Helmínticos/química , Antígenos Helmínticos/genética , Clonación Molecular , Citocinas/inmunología , Filariasis/inmunología , Filariasis/parasitología , Humanos , Larva/inmunología , Datos de Secuencia Molecular , Alineación de Secuencia , Vacunas/inmunología , Wuchereria bancrofti/inmunologíaRESUMEN
Helminth parasites use antioxidant defence strategies for survival during oxidative stress due to free radicals in the host. Accordingly, tissue-dwelling filarial parasites counteract host responses by releasing a number of antioxidants. Targeting these redox regulation proteins together, would facilitate effective parasite clearance. Here, we report the combined effect of protective immune responses trigged by recombinant Wuchereria bancrofti thioredoxin (WbTRX) and thioredoxin peroxidase (WbTPX) in an experimental filarial model. The expression of WbTRX and WbTPX in different stages of the parasite and their cross-reactivity were analysed by enzyme-linked immunosorbent assay (ELISA). The immunogenicity of recombinant proteins and their protective efficacy were studied in animal models when immunized in single or cocktail mode. The antigens showed cross-reactive epitopes and induced high humoral and cellular immune responses in mice. Further, parasite challenge against Brugia malayi L3 larvae in Mastomys coucha conferred significant protection of 57% and 62% against WbTRX and WbTPX respectively. The efficacy of L3 clearance was significantly higher (71%) (P < 0.001) when the antigens were immunized together, showing a synergistic effect in multiple-mode vaccination. Hence, the study suggests WbTRX and WbTPX to be attractive vaccine candidates when immunized together and provides a tandem block for parasite elimination in the control of lymphatic filariasis.
Asunto(s)
Antioxidantes/metabolismo , Filariasis/inmunología , Peroxirredoxinas/metabolismo , Tiorredoxinas/metabolismo , Wuchereria bancrofti/enzimología , Animales , Anticuerpos Antihelmínticos , Antígenos Helmínticos , Femenino , Masculino , Ratones , Ratones Endogámicos BALB C , Murinae , Oxidación-Reducción , Peroxirredoxinas/inmunología , Proteínas Recombinantes , Tiorredoxinas/inmunologíaRESUMEN
As in many other parasitic diseases, efficacious vaccine for lymphatic filariasis has been elusive for want of new approaches leaving billions of people either debilitated or at risk. With multiple B- and T-cell epitopes, the abundant larval transcript-2 (ALT-2) of the filarial worm, Brugia malayi, has been shown to be a promising immunoprophylactic target. To enhance its efficacy, it was lipid modified using our recently developed protein engineering tool, which then offered 30% more immunoprotection (49 vs. 79%) in Mastomys coucha model. Sustained high levels of IFN-γ (about 100 times) and high antibody titres (10-fold) elicited by lipid-modified ALT-2, as compared to the native form, indicated the maintenance of Th1/Th2 balance that is impaired in filariasis. Thus, this study provides the basis for developing efficacious vaccines for filariasis and other parasitic diseases by exploiting bacterial lipid modification.
Asunto(s)
Antígenos Helmínticos/inmunología , Brugia Malayi/inmunología , Filariasis Linfática/prevención & control , Lípidos/inmunología , Animales , Anticuerpos Antihelmínticos/sangre , Anticuerpos Antihelmínticos/inmunología , Citocinas/inmunología , Citocinas/metabolismo , Filariasis Linfática/inmunología , Epítopos de Linfocito T/inmunología , Escherichia coli/genética , Glicéridos/inmunología , Humanos , Inmunización , Larva/inmunología , Activación de Linfocitos , Masculino , Murinae , Ingeniería de Proteínas , Proteínas Recombinantes/inmunología , Linfocitos T/inmunología , Vacunas/inmunologíaRESUMEN
The ability of nematode parasites to survive in a highly complex immune system involves diverse strategies including production of a variety of host immune modulators. Various parasite-associated surface antigens or excretory and secretory products may possibly play a role in the host-parasite interactions and successful survival of parasite in their respective host. One among these molecules is a human cytokine homolog, macrophage migration inhibitory factor-1 (MIF-1) in various parasites. We identified a homolog of this cytokine from human lymphatic filarial parasite, Wuchereria bancrofti, expression cloned and investigated its molecular characteristics and catalytic properties. We also assessed the humoral reactivity of the recombinant MIF-1 of W. bancrofti (rWb-MIF-1) against sera belonging to different categories of individuals viz. microfilaremic, chronic patients, endemic normal, and non-endemic normal. Our results showed that the complete coding sequence of W. bancrofti is 1,078 bp, comprising two introns and three exons: first and second introns being 577 and 153 bp long, while the three exons I, II, and III being 108, 173, and 67 bp long, respectively. The rWb-MIF-1 was overexpressed in a salt-inducible host, Escherichia coli GJ 1158, and its functional activity was determined by dopachrome tautomerase and insulin reduction assays. The results of both the assays showed that the purified protein is functionally active and hence folded appropriately. The rWb-MIF-1 protein did not show elevation of specific IgG4 antibodies in microfilaremic cases, a hallmark in case of lymphatic filariasis, while it showed IgE reactivity in some of these cases (five out of ten).
Asunto(s)
Proteínas del Helminto/genética , Proteínas del Helminto/metabolismo , Factores Inhibidores de la Migración de Macrófagos/genética , Factores Inhibidores de la Migración de Macrófagos/metabolismo , Wuchereria bancrofti/genética , Animales , Anticuerpos Antihelmínticos/sangre , Clonación Molecular , Filariasis Linfática/inmunología , Filariasis Linfática/parasitología , Escherichia coli/genética , Exones , Expresión Génica , Humanos , Inmunoglobulina E/sangre , Intrones , Sistemas de Lectura Abierta , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Wuchereria bancrofti/aislamiento & purificaciónRESUMEN
In this study filarial recombinant protein or DNA vaccine constructs encoding BmALT-2 and BmVAH as single or as cocktail antigens were evaluated. Male jirds were immunized intramuscularly with DNA vaccine constructs or were immunized intraperitoneally with protein vaccine. The single and bicistronic DNA constructs induced substantial interferon-γ responses in spleen cells; antigen-specific responses were higher following immunization with the bicistronic cocktail construct and evoked a significant protective response of 57% in jirds challenged with Brugia malayi that was similar in the antibody-dependent cellular cytotoxicity (ADCC) assay and micropore chamber experiment. The cocktail protein vaccines induced a mixture of IgG2a (Th1) and IgG1 (Th2) responses with 80% protective response when challenged with B. malayi infective larvae. However, the single protein vaccine rALT-2 induced Th2 (IgG1/IgG3) with a 70% protective response and rVAH induced Th1 (IgG2a) with a lower proliferative response with 60% protection following challenge with B. malayi infective larvae. These results suggest that filarial cocktail protein vaccines are able to elicit substantial immune and protective responses when compared with single antigen vaccination in suitably vaccinated jirds.
Asunto(s)
Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/inmunología , Brugia Malayi/inmunología , Filariasis Linfática/prevención & control , Gerbillinae/inmunología , Proteínas Recombinantes/inmunología , Vacunas de ADN/inmunología , Animales , Antígenos Helmínticos/genética , Antígenos Helmínticos/metabolismo , Brugia Malayi/genética , Brugia Malayi/patogenicidad , Células CHO , Cricetinae , Modelos Animales de Enfermedad , Filariasis Linfática/inmunología , Inmunización , Inmunoglobulina G/sangre , Masculino , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Vacunas de ADN/administración & dosificación , Vacunas de ADN/genéticaRESUMEN
The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.
Asunto(s)
Anticuerpos Antihelmínticos , Anticuerpos Monoclonales , Antígenos Helmínticos/sangre , Filariasis Linfática/diagnóstico , Wuchereria bancrofti/inmunología , Animales , Anticuerpos Antihelmínticos/biosíntesis , Anticuerpos Antihelmínticos/inmunología , Anticuerpos Monoclonales/biosíntesis , Anticuerpos Monoclonales/inmunología , Especificidad de Anticuerpos , Antígenos Helmínticos/genética , Antígenos Helmínticos/inmunología , Filariasis Linfática/inmunología , Filariasis Linfática/parasitología , Ensayo de Inmunoadsorción Enzimática , Proteínas del Helminto/genética , Proteínas del Helminto/inmunología , Humanos , Ratones , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Homología de Secuencia de AminoácidoRESUMEN
Filarial nematodes down-regulate the host immune response to establish infection by inducing IL-10-mediated T-cell suppression.Abundant larval transcript (ALT) proteins are of major interest as they are expressed abundantly in the L3 stage, implicated in protective immunity and may play a role in immune evasion. The T-cell epitopes of BmALT-2 and the cytokine responses induced by these peptides were investigated in a mouse model using synthetic peptides, which could be exploited in the design of a potent epitope-based vaccine. Five regions were found to carry T-cell epitopes inducing high levels of cellular proliferation. The regions 5568 and 7391 of ALT-2 induced very high levels of IL-10 secretion and hence could be involved in immunomodulation in the host.
Asunto(s)
Antígenos Helmínticos/inmunología , Brugia Malayi/inmunología , Citocinas/metabolismo , Epítopos de Linfocito T/inmunología , Epítopos Inmunodominantes/inmunología , Proteínas Recombinantes/inmunología , Animales , Femenino , Ratones , Ratones Endogámicos BALB CRESUMEN
Lymphatic filariasis is a tropical disease, with over 40 million people seriously incapacitated due to lymphangitis and elephantiasis. Over 99% of infections are caused by the nematode Wuchereria bancrofti. Expressed sequence tag (EST) analysis of filarial genome identified novel infective larval (L3) stage-specific antigen, abundant larval transcript (ALT-2), which was shown to be highly essential for parasite establishment and survival in the host. The unique structural features and immunological characteristics of ALT-2 indicate the presence of critical motifs that needs to be explored in natural human infection for understanding and management of the disease. In order to dissect the epitopes of ALT protein recognized in humans, eight peptides spanning the entire protein sequence were selected based on in-silico epitope prediction and synthesized. Analysis of the reactivity of W. bancrofti ALT-2 synthetic peptides with clinical sera (n = 40) from endemic areas identified epitopes recognized by putatively immune sera, of which two comprise the highly variable acidic domain (21-60). Interestingly, our study also revealed crucial epitopes recognized by microfilaremic (MF) sera with significantly high IgG4 isotype (p < 0.001), implicated in immunomodulation. The epitope peptides identified were highly specific for MF sera and, thus, have the potential to be exploited as diagnostic markers.
Asunto(s)
Antígenos Helmínticos/inmunología , Filariasis Linfática/inmunología , Epítopos/inmunología , Wuchereria bancrofti/crecimiento & desarrollo , Wuchereria bancrofti/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos Antihelmínticos/sangre , Anticuerpos Antihelmínticos/inmunología , Antígenos Helmínticos/química , Filariasis Linfática/parasitología , Epítopos/química , Proteínas del Helminto/química , Proteínas del Helminto/inmunología , Humanos , Inmunoglobulina G/sangre , Isotipos de Inmunoglobulinas , Larva/inmunología , Ratones , Datos de Secuencia Molecular , Péptidos/síntesis química , Péptidos/química , Wuchereria bancrofti/patogenicidadRESUMEN
Transgenic tobacco plants were developed expressing WbSXP-1, a diagnostic antigen isolated from the cDNA library of L3 stage larvae of Wucheraria bancrofti. This antigen produced by recombinant Escherichia coli has been demonstrated by to be successful as potential diagnostic candidate against lymphatic filariasis. A rapid format simple and qualitative flow through immune-filtration diagnostic kit has been developed for the identification of IgG antibodies to the recombinant WbSXP-1 and is being marketed by M/S Span Diagnostics Ltd in India and Africa. Here, we present the results of experiments on the transformation and expression of the same filarial antigen, WbSXP-1, in tobacco plant, Nicotiana tabacum, to produce plant-based diagnostic antigen. It was possible to successfully transform the tobacco plant with WbSXP-1, the integration of the parasite-specific gene in plants was confirmed by PCR amplification and the expression of the filarial protein by Western blotting. The immunoreactivity of the plant-produced WbSXP-1 was assessed based on its reaction with the monoclonal antibodies developed against the E. coli-produced protein. Immunological screening using clinical sera from patients indicates that the plant-produced protein is comparable to E. coli-produced diagnostic antigen. The result demonstrated that plants can be used as suitable expression systems for the production of diagnostic proteins against lymphatic filariasis, a neglected tropical infectious disease which has a negative impact on socioeconomic development. This is the first report of the integration, expression and efficacy of a diagnostic candidate of lymphatic filariasis in plants.Key MessageTransgenic tobacco plants with WbSXP-1, a filarial diagnostic candidate, were developed. The plant-produced protein showed immunoreactivity on par with the E. coli product.
Asunto(s)
Filariasis Linfática/diagnóstico , Filariasis Linfática/inmunología , Ingeniería Genética/métodos , Proteínas del Helminto/genética , Nicotiana/genética , Wuchereria/genética , Animales , Clonación Molecular , Ensayo de Inmunoadsorción Enzimática , Femenino , Expresión Génica , Humanos , Ratones , Plantas Modificadas Genéticamente , Reacción en Cadena de la Polimerasa , Transformación GenéticaRESUMEN
We have cloned homologues of the mammalian translationally controlled tumor protein (TCTP) from the human filarial parasites Wuchereria bancrofti and Brugia malayi. TCTP genes from B. malayi and W. bancrofti were expressed in a T7 promoter vector as histidine tagged fusion proteins. Both the recombinant B. malayi TCTP (rBm-TCTP) and recombinant W. bancrofti TCTP (rWb-TCTP) have a molecular mass of approximately 28 kDa with the histidine tag. Sequence analyses showed that there is a 98% similarity between the two filarial TCTPs at amino acid levels and are immunologically cross-reactive. Analysis of soluble proteins from various lifecycle stages of B. malayi suggested that the expression of Bm-TCTP might be differentially regulated and occurs in multimeric form. Recombinant TCTP were found to form multimers in solution under non-reducing conditions. The tendency for filarial TCTPs to become multimers was predicted by the presence of the Lupas coiled coil structure in their sequence. Despite the absence of a signal sequence, Bm-TCTP is present abundantly in the excretory/secretions (ES) of microfilariae. Characterization studies showed that both Bm- and Wb-TCTPs are calcium-binding proteins and have histamine-releasing function in vitro. When injected intraperitoneally both the filarial TCTPs induced inflammatory infiltration of eosinophils into the peritoneal cavity of mice suggesting that the filarial TCTPs may have a role in the allergic inflammatory responses associated with filarial infections.
Asunto(s)
Biomarcadores de Tumor , Brugia Malayi/metabolismo , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Calcio/metabolismo , Wuchereria bancrofti/metabolismo , Secuencia de Aminoácidos , Animales , Brugia Malayi/genética , Brugia Malayi/crecimiento & desarrollo , Proteínas de Unión al Calcio/química , Proteínas de Unión al Calcio/inmunología , Filariasis/inmunología , Filariasis/parasitología , Liberación de Histamina , Humanos , Hipersensibilidad/etiología , Hipersensibilidad/inmunología , Ratones , Datos de Secuencia Molecular , Peritoneo/citología , Peritoneo/inmunología , Ratas , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Análisis de Secuencia de ADN , Células Tumorales Cultivadas , Proteína Tumoral Controlada Traslacionalmente 1 , Wuchereria bancrofti/genética , Wuchereria bancrofti/crecimiento & desarrolloRESUMEN
The gene encoding the Wuchereria bancrofti orthologue of the Brugia malayi-derived diagnostic antigen SXP1 was identified from a W. bancrofti L3 cDNA library and characterized. The Wb-sxp-1 cDNA encoded a basic protein with a calculated molecular mass of 20.8 kDa. Wb-SXP-1 was 85% identical to the SXP1 protein described from B. malayi (Bm-SXP-1). The Wb-SXP-1 sequence also showed significant identity with proteins described from B. pahangi, Onchocerca volvulus, Acanthochilonema vitea, Ascaris suum, Loa loa, Litomosoides sigmodontis and Caenorhabditis elegans. The presence of a number of invariant and conserved residues in all of these nematode-derived molecules suggests that Wb-SXP-1 is a member of a new protein family. A recombinant form of Wb-SXP-1 was produced and it was determined that the anti-Wb-SXP-1 antibody response in patients with W. bancrofti infections was restricted to the IgG4 subclass. An anti-Wb-SXP-1 IgG4 ELISA was developed and this assay was found to be 100% sensitive for patients with patent W. bancrofti infection. Sera from individuals experiencing chronic pathology, endemic normals or patients with non-filarial nematode infections had no detectable IgG4 against Wb-SXP-1. While patients with patent Onchocerca volvulus infections were uniformly negative in the Wb-SXP-1 assay, 40% of sera from patent Loa loa infections were positive. When Bm-SXP-1 was used as the antigen under identical conditions, the assay was 88% specific for patent W. bancrofti infections and the antigen was recognized by antibodies from both O. volvulus and L. loa infections. The results strongly suggested that, for certain diagnostic filarial antigens, the use of same-species molecules can enhance the specificity of diagnostic tests.
Asunto(s)
Antígenos Helmínticos/aislamiento & purificación , Brugia Malayi/inmunología , Filariasis/diagnóstico , Filariasis/inmunología , Proteínas del Helminto/aislamiento & purificación , Wuchereria bancrofti/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/genética , Brugia Malayi/genética , Proteínas del Helminto/genética , Proteínas del Helminto/inmunología , Humanos , Inmunoglobulina G/biosíntesis , Inmunoglobulina G/sangre , Inmunoglobulina G/clasificación , Datos de Secuencia Molecular , Proteínas Recombinantes/biosíntesis , Homología de Secuencia de Aminoácido , Wuchereria bancrofti/genéticaRESUMEN
The indirect hemagglutination test (IHAT), indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been applied to the serodiagnosis of bancroftian filariasis employing Wuchereria bancrofti mf antigens and the efficiency of these tests in the detection of antibody has been compared. Each test was found to be marginally superior to the other two tests with a particular group of endemic sera for the detection of filarial antibody. In other words, the IHAT, IFAT and ELISA showed a greater number of positive reactions with endemic normal, microfilaremia and clinical filarial sera, respectively. ELISA is simple, sensitive and can be used in seroepidemiological studies for bancroftian filariasis employing soluble antigens.
Asunto(s)
Filariasis/diagnóstico , Antígenos/inmunología , Ensayo de Inmunoadsorción Enzimática , Filariasis/inmunología , Técnica del Anticuerpo Fluorescente , Pruebas de Hemaglutinación , Humanos , Microfilarias/inmunología , Wuchereria bancrofti/inmunologíaRESUMEN
Infections with Wuchereria bancrofti causing lymphatic filariasis still represent one of the major health problems in the tropics, with 120 million people infected and over 750 million exposed to this filarial parasite. We have studied lymphatic filariasis infections as part of a multi-parasite survey in a village community in the savannah of northern Nigeria. We analysed serum samples from 341 individuals aged 5-70 years, detecting a W. bancrofti circulating antigen using the commercially available ICT Filariasis card test. The prevalence of infections was 10% and clearly age-dependent, increasing from below 2% in children to over 20% in subjects older than 40 years. Measuring IgG4 antibodies against the recombinant W. bancrofti antigen SXP1 showed that 36% of all tested individuals had been at least exposed to the parasite. Antibody levels also increased very significantly with age. A further analysis measuring Onchocerca volvulus-specific IgG4 antibodies showed a very significant association between infections with O. volvulus and those with W. bancrofti. Our data show that infections with W. bancrofti in Nigeria are still a frequently occurring health problem, since they are more prevalent than previously reported, and that individuals with an O. volvulus infection are more often infected with W. bancrofti than expected statistically.
Asunto(s)
Filariasis/epidemiología , Onchocerca volvulus , Oncocercosis/complicaciones , Wuchereria bancrofti , Anciano , Animales , Anticuerpos Antihelmínticos/análisis , Preescolar , Estudios Transversales , Ensayo de Inmunoadsorción Enzimática , Femenino , Filariasis/complicaciones , Filariasis/diagnóstico , Humanos , Inmunoglobulina G/análisis , Masculino , Nigeria/epidemiología , Onchocerca volvulus/aislamiento & purificación , Oncocercosis/diagnóstico , Oncocercosis/parasitología , Prevalencia , Especificidad de la Especie , Wuchereria bancrofti/patogenicidadRESUMEN
A simple, sensitive and stable ELISA (enzyme linked immunosorbent assay) was developed using rabbit antibody to fractionated Entamoeba histolytica antigen for the detection of copro antigen in the faeces of individuals with intestinal amoebiasis. In this test none of the healthy individuals, all trophozoite positive, 40% cyst passers and 6% individuals living in endemic area showed the presence of copro antigen. ELISA using polyclonal rabbit antibody could detect 1-5 trophozoites/well and 20-50 ng protein per well of NIH-200 strain of E. histolytica and the sensitivity of the test was comparable with that using monoclonal antibody. Cross reaction was observed only with E. invadens when faeces having other parasites were screened. The reagents of ELISA were stabilized and found to be stable for more than 6 months when stored at 4 degrees C.
Asunto(s)
Antígenos de Protozoos/análisis , Disentería Amebiana/inmunología , Ensayo de Inmunoadsorción Enzimática/métodos , Animales , Anticuerpos Antiprotozoarios/inmunología , Reacciones Cruzadas , Entamoeba/inmunología , Entamoeba histolytica/inmunología , Heces , Humanos , ConejosRESUMEN
Entamoeba histolytica soluble crude antigen was fractionated by gel filtration on Sephacryl S-300 into four fractions, viz. F1(669 kDa); F2(51.2 kDa); F3(25.1 kDa) and F4(10.5 kDa). F1 fraction was observed to be more sensitive and specific for the detection of antibody in amoebiasis than the crude and other fractions of purified antigens employing IHAT and ELISA. ELISA was found to be better than IHAT since it could detect antibody in the sera (3/6) of asymptomatic cyst passers. The cross reaction of crude antigen with toxocariasis (1/4) and toxoplasmosis (2/5) sera were associated with F4 fraction. F3 and F4 were having low molecular weight and were not sensitive in detection of antibody in amoebiasis. Biochemical characterization revealed glycoprotein nature of the specific (F1) antigen fraction.