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1.
Nucleic Acids Res ; 50(14): 8143-8153, 2022 08 12.
Artículo en Inglés | MEDLINE | ID: mdl-35801908

RESUMEN

Tandem repeats of guanine-rich sequences in RNA often form thermodynamically stable four-stranded RNA structures. Such RNA G-quadruplexes have long been considered to be linked to essential biological processes, yet their physiological significance in cells remains unclear. Here, we report a approach that permits the detection of RNA G-quadruplex structures that modulate protein translation in mammalian cells. The approach combines antibody arrays and RGB-1, a small molecule that selectively stabilizes RNA G-quadruplex structures. Analysis of the protein and mRNA products of 84 cancer-related human genes identified Nectin-4 and CapG as G-quadruplex-controlled genes whose mRNAs harbor non-canonical G-quadruplex structures on their 5'UTR region. Further investigations revealed that the RNA G-quadruplex of CapG exhibits a structural polymorphism, suggesting a possible mechanism that ensures the translation repression in a KCl concentration range of 25-100 mM. The approach described in the present study sets the stage for further discoveries of RNA G-quadruplexes.


Asunto(s)
G-Cuádruplex , Regiones no Traducidas 5' , Animales , Guanina/química , Humanos , Mamíferos/genética , Biosíntesis de Proteínas , ARN Mensajero/metabolismo
2.
Biochem Biophys Res Commun ; 526(1): 261-266, 2020 05 21.
Artículo en Inglés | MEDLINE | ID: mdl-32209257

RESUMEN

The last several years have seen exciting advances in the understanding of the structure and function of higher-order structures of RNA. Expression levels of some specific genes were shown to be directly regulated by environmentally-responsive formation of certain secondary structures such as stem-loops and pseudoknots. Even among these noncanonical structures, RNA G-quadruplexes, which form on the regions of guanine-rich sequences in mRNA, are highly stable structures that are involved in a variety of biological processes. However, many questions regarding the biological significance of RNA G-quadruplexes remain unsettled, mainly because it is difficult to locate the structures in mRNA. This review focuses on emerging methods that locate RNA G-quadruplexes in mRNA by computational and biochemical techniques. In addition, recent reports on the biological functions of RNA G-quadruplexes are also covered to highlight their various roles in cells, such as in regulating mRNA processing and translation.


Asunto(s)
G-Cuádruplex , ARN Mensajero/genética , Regiones no Traducidas 5'/genética , Empalme Alternativo/genética , Precursores del ARN/metabolismo , Transporte de ARN/genética , ARN Mensajero/metabolismo , Transcripción Reversa/genética
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