Integration between swim speed and mouth size evolves repeatedly in Trinidadian guppies and aligns with suction-feeding fishes.

Well-supported correlations between swim speed and mouth size during prey capture suggest the broad existence of an integrated relationship between locomotion and feeding in suction-feeding fishes. However, the influence of specialization on this relationship is unclear. We used divergent populations of Trinidadian guppies (Poecilia reticulata) to test whether integration during suction is generalizable to a non-suction specialist and whether intraspecific specialization of component systems affects their integration. Guppies from replicate high- and low-predation streams were recorded capturing wild-type zooplankton using suction. Alternative general linear models supported a positive correlation between swim speed and mouth size in derived low-predation populations, suggesting that the relationship can be extended in some cases. High-predation populations lack this integration, which may be the result of direct selection or constraints imposed by selection on locomotion. As guppies invade new habitats they may be evolving a new, integrated performance phenotype from a non-integrated ancestor.

Correlation of MRI Findings With Patterns of Visual Field Loss in Patients With Pituitary Tumors.

BACKGROUND: Compression of the optic chiasm by pituitary tumors typically results in bitemporal hemianopia, implying that nasal retinal fibers are preferentially damaged. The reason for this is not clear. One theory suggests that nasal fibers are selectively vulnerable simply because they cross each other. This study investigated the "crossing theory" by correlating visual field (VF) loss with chiasmal elevation and with the degree of eccentric compression on MRI scans. METHODS: Our hospital database was searched to identify patients with a) chiasmal compression by a pituitary tumor; b) documented preoperative evidence of VF loss; and c) preoperative MRI scan performed within 1 month of VF testing. Temporality and bitemporality indices were derived from pattern deviation VF plots. Elevations of the central and peripheral parts of the chiasm were obtained from MRI scans, from which the eccentricity of compression was calculated. Temporality indices and hemifield loss were compared with central chiasmal elevation, and nasal hemifield loss in each eye was plotted against eccentricity. RESULTS: Eleven patients were suitable for analysis. The degree of bitemporal VF involvement was significantly correlated with elevation of the central chiasm (P = 0.004). However, there was minimal involvement of nasal VFs, and no demonstrable increase in nasal field loss with increasing eccentricity of compression. CONCLUSIONS: This study provides support for the crossing theory. These findings will inform further finite element models of chiasmal compression. A larger, prospective study is planned.

Reducing Physical Restraint Use in the Medical Behavioral Unit.

OBJECTIVES: Children with behavioral health conditions often experience agitation when admitted to children's hospitals. Physical restraint should be used only as a last resort for patient agitation because it endangers the physical and psychological safety of patients and employees. At the medical behavioral unit (MBU) in our children's hospital, we aimed to decrease the weekly rate of physical restraint events per 100 MBU patient-days, independent of patient race, ethnicity, or language, from a baseline mean of 14.0 to <10 within 12 months. METHODS: Using quality improvement methodology, a multidisciplinary team designed, tested, and implemented interventions including a series of daily deescalation huddles led by a charge behavioral health clinician that facilitated individualized planning for MBU patients with the highest behavioral acuity. We tracked the weekly number of physical restraint events per 100 MBU patient-days as a primary outcome measure, weekly physical restraint event duration as a secondary outcome measure, and MBU employee injuries as a balancing measure. RESULTS: Our cohort included 527 consecutive patients hospitalized in the MBU between January 2021 and January 2023. Our 2021 baseline mean of 14.0 weekly physical restraint events per 100 MBU patient-days decreased to 10.0 during our 2022 intervention period from January through July and 4.1 in August, which was sustained through December. Weekly physical restraint event duration also decreased from 112 to 67 minutes without a change in employee injuries. CONCLUSIONS: Multidisciplinary huddles that facilitated daily deescalation planning safely reduced the frequency and duration of physical restraint events in the MBU.

Battle of the Bites: The Effect of Sewage Effluent Exposure on Mosquitofish Biocontrol of Mosquitoes in Residential Louisiana.

Mosquitofish, Gambusia affinis, are eponymous larval mosquito predators. Their ability to colonize and survive in habitats that are uninhabitable by other potential predators allows them to naturally manage larval mosquito populations in most ground pools they are present in. However, effluent from residential onsite wastewater treatment systems (OWTSs) appears to limit the presence of fish predators. This is especially problematic in Louisiana, where regulations allow the discharge of OWTS effluent into open drainage conveyances. To determine the effect of effluent on the capacity of mosquitofish for biocontrol in contaminated areas, we assessed the body condition metrics of populations from two effluent-exposed sites and two sites not exposed to effluent, determined the lethal effect of effluent-contaminated drainage water on fish, and measured the prey consumption rates in the presence of effluent. Female fish collected from effluent-impacted sites had a reduced somatic body condition and most females examined displayed masculinized anal fins resembling the male gonopodium structure. This trait was not seen in fish collected from the control sites and has not yet been documented in association with OWTSs or in the state of Louisiana. Fish from the control sites survived at effluent-contaminated water levels < 70%, and the prey clearance rates increased with dilution. Onsite wastewater treatment system effluent has significant effects on both the short- and long-term persistence of mosquitofish, their body composition, reproductive health, and larval mosquito consumption. These effects likely release mosquito larvae from suppression and may increase the threat of mosquito-transmitted pathogens in effluent-contaminated locations.

An Emergency Department Quality Improvement Project to Decrease Lumbar Puncture Rates in Febrile Infants 22 to 28 Days Old.

Introduction: Most providers have routinely performed universal lumbar puncture (LP) on well-appearing, febrile infants 22 to 28 days old. In 2021, the American Academy of Pediatrics recommended clinicians should perform an LP in this age group if inflammatory markers are abnormal. This quality improvement project aimed to decrease LP rates in febrile infants 22 to 28 days old in the emergency department (ED) within 1 year, regardless of race/ethnicity, from a baseline of 87%. Methods: We used our institution's quality improvement framework to perform multiple Plan-Do-Study-Act cycles. A multidisciplinary team reviewed the febrile infant literature, local epidemiology, and identified key drivers. We provided departmental education, updated our clinical pathway, and used clinical decision support. We analyzed baseline (January 2017-March 2022) and intervention data (April 2022-March 2024) and tracked data using statistical process control charts. Our primary outcome measure was rates of LP in the ED for this cohort. Process measures included rates of infants with procalcitonin results. ED length of stay, rates of first LP attempt after hospitalization, and missed bacterial meningitis were balancing measures. Results: The baseline LP rate of 87% decreased to 44% during the intervention period, resulting in a downward centerline shift. There were no significant differences when LP rates were analyzed by race/ethnicity. There was an upward centerline shift in the process measure of infants with procalcitonin results. There was no observed special cause variation in our balancing measures. Conclusion: Quality improvement efforts, including education, clinical pathway updates, and clinical decision support, safely reduced rates of LPs in febrile infants 22 to 28 days old.

Intraspecific variation in feeding and locomotor kinematics during prey capture in redbreast sunfish (Lepomis auritus).

Biomechanics research often revolves around understanding traits impacting suction feeding performance in fishes, using freshwater ray-finned sunfishes (Family Centrarchidae) as models. However, simultaneous feeding and locomotion kinematics during prey capture are not recorded for many species and there is less information on how these kinematics vary within a species and within individuals. To (1) add to existing data on the prey capture kinematics of centrarchids, (2) assess variation in a species both within and across individuals, and (3) compare morphology and prey capture kinematics of well-sampled centrarchids, we filmed five redbreast sunfish (Lepomis auritus) at 500 fps-1 approaching and striking non-evasive prey. Redbreast approach prey at ~30 cm s-1 and use approximately 70% of their maximum gape size. Traits related to feeding are more repeatable than traits related to locomotion. However, the Accuracy Index (AI) was consistent across individuals (AI = 0.76 ± 0.07). Functionally, redbreast sunfish are more similar to bluegill sunfish but morphologically they fall in the intermediate morphospace alongside green sunfish when compared with other centrarchids. These data show that whole organism outcomes (AI) are similar despite variation present both within and across individuals and demonstrate the importance of considering both interspecific and intraspecific differences in the functional diversity of ecologically and evolutionarily important behaviors such as prey capture.

Characteristics, disposition, and outcomes of children hospitalized for mental health boarding at a children's hospital.

Increasingly, youth experiencing mental health crises present to acute care medical hospitals and "board" on medical units due to inpatient psychiatric bed shortages. We conducted a retrospective cohort study of children experiencing mental health boarding at a US children's hospital from October 2020 to September 2022. We examined associations between patients' characteristics and their disposition and outcomes. Our cohort included 1891 boarding hospitalizations: 53.9% transferred to an inpatient psychiatric hospital and 46.1% discharged home. Characteristics associated with not being transferred to an inpatient psychiatric hospital included age <13 years (adjusted odds ratio [aOR] 0.6; 95% confidence interval [CI]: 0.4-0.7), disruptive or aggressive behavior (aOR 0.6; 95% CI: 0.4-0.8), psychosis (aOR 0.5; 95% CI: 0.3-0.8), COVID-19 infection (aOR 0.3; 95% CI: 0.2-0.6), or a complex chronic medical condition (aOR 0.8; 95% CI: 0.6-1.0). Our findings suggest that certain populations of children experiencing mental health boarding face disparate access to inpatient psychiatric care.

The ontogenetic scaling of bite force and head size in loggerhead sea turtles (Caretta caretta): implications for durophagy in neritic, benthic habitats.

Ontogenetic studies of vertebrate feeding performance can help address questions relevant to foraging ecology. Feeding morphology and performance can either limit access to food resources or open up new trophic niches in both aquatic and terrestrial systems. Loggerhead sea turtles are long-lived vertebrates with complex life histories that are marked by an ontogenetic shift from an oceanic habitat to a coastal neritic habitat, and a transition from soft oceanic prey to hard, benthic prey. Although considered durophagous and strong biters, bite performance has not been measured in loggerheads, nor has the ontogeny of bite performance been characterized. In the present study, we collected measurements of bite force in loggerhead turtles from hatchlings to adults. When subadults reach the body size at which the ontogenetic shift occurs, their crushing capability is great enough for them to consume numerous species of hard benthic prey of small sizes. As loggerheads mature and bite performance increases, larger and harder benthic prey become accessible. Loggerhead bite performance eventually surpasses the crushing capability of other durophagous carnivores, thereby potentially reducing competition for hard benthic prey. The increasing bite performance and accompanying changes in morphology of the head and jaws are likely an effective mechanism for resource partitioning and decreasing trophic competition. Simultaneous measurements of body and head size and the use of non-linear reduced major axis regression show that bite force increases with significant positive allometry relative to body size (straight carapace length, straight carapace width and mass) and head size (head width, height and length). Simple correlation showed that all recorded morphometrics were good predictors of measured bite performance, but an AICc-based weighted regression showed that body size (straight carapace width followed by straight carapace length and mass, respectively) were more likely predictors of bite force than head size morphometrics (head width and head length).

Veterinary needs for animal cruelty recognition and response in the United States center on training and workplace policies.

OBJECTIVE: To better understand the experiences and perceptions of veterinary professionals in the US responding to suspected cases of animal cruelty. SAMPLE: 1,027 US veterinary professionals. PROCEDURES: An anonymous survey was distributed via social media and veterinary professional associations between October 15 and November 15, 2020. RESULTS: Responses indicated that more cases of suspected animal cruelty were seen by those who reported having had cruelty training and in workplaces with emergency intake or a relationship with law enforcement. In addition, suspected cases were more likely to be reported to authorities by respondents who indicated there was a workplace policy for handling cruelty cases and by individuals who indicated that they were aware that they were mandated reporters. CLINICAL RELEVANCE: The important needs for veterinary professionals in relation to animal cruelty are more training to recognize animal cruelty, workplace policies for reporting, and knowledge of reporting laws.

The integration of locomotion and prey capture in divergent cottid fishes: functional disparity despite morphological similarity.

Many mobile animals rely on the integration of locomotion and feeding to capture prey. Fishes commonly swim up to a prey item and utilize a combination of ram and suction feeding for prey capture. Marine cottids represent a diverse and abundant lineage of fishes that exhibit variation in feeding mode that is related to their mouth morphology. However, little is known regarding the integration of the locomotor and feeding systems during prey capture. We quantified the feeding kinematics, feeding performance and integration of locomotion and feeding in two species of divergent cottids: Blepsias cirrhosus (silver-spotted sculpin) and Oligocottus maculosus (tidepool sculpin). Individuals were caught from sympatric habitats near the Bamfield Marine Sciences Centre on Vancouver Island and filmed with a high-speed video camera (500 Hz) while feeding on amphipod prey. Two principal component axes summarize differences in integration and feeding mode despite similarity in attack velocity and feeding morphology (peak gape, peak cranial elevation and peak jaw protrusion). A greater number of correlations between locomotor and feeding variables in B. cirrhosus, compared with O. maculosus, indicate greater integration. We conclude that traditional measures of attack kinematics do not capture functionally and ecologically relevant differences between species. The mechanisms underlying differences in locomotor strategy likely result from unexplored morphological or ecological differences between species. In cottids, integration is apparent in more basal, subtidal species such as B. cirrhosus, and the need for integration may be superceded by demands from the habitat in more derived, shallow-water species such as O. maculosus.

Ocean forcing drives glacier retreat in Greenland.

The retreat and acceleration of Greenland glaciers since the mid-1990s have been attributed to the enhanced intrusion of warm Atlantic Waters (AW) into fjords, but this assertion has not been quantitatively tested on a Greenland-wide basis or included in models. Here, we investigate how AW influenced retreat at 226 marine-terminating glaciers using ocean modeling, remote sensing, and in situ observations. We identify 74 glaciers in deep fjords with AW controlling 49% of the mass loss that retreated when warming increased undercutting by 48%. Conversely, 27 glaciers calving on shallow ridges and 24 in cold, shallow waters retreated little, contributing 15% of the loss, while 10 glaciers retreated substantially following the collapse of several ice shelves. The retreat mechanisms remain undiagnosed at 87 glaciers without ocean and bathymetry data, which controlled 19% of the loss. Ice sheet projections that exclude ocean-induced undercutting may underestimate mass loss by at least a factor of 2.

Risk Factors and Outcomes After a Brief Resolved Unexplained Event: A Multicenter Study.

BACKGROUND: The accuracy of the risk criteria for brief resolved unexplained events (BRUEs) from the American Academy of Pediatrics (AAP) is unknown. We sought to evaluate if AAP risk criteria and event characteristics predict BRUE outcomes. METHODS: This retrospective cohort included infants <1 year of age evaluated in the emergency departments (EDs) of 15 pediatric and community hospitals for a BRUE between October 1, 2015, and September 30, 2018. A multivariable regression model was used to evaluate the association of AAP risk factors and event characteristics with risk for event recurrence, revisits, and serious diagnoses explaining the BRUE. RESULTS: Of 2036 patients presenting with a BRUE, 87% had at least 1 AAP higher-risk factor. Revisits occurred in 6.9% of ED and 10.7% of hospital discharges. A serious diagnosis was made in 4.0% (82) of cases; 45% (37) of these diagnoses were identified after the index visit. The most common serious diagnoses included seizures (1.1% [23]) and airway abnormalities (0.64% [13]). Risk is increased for a serious underlying diagnosis for patients discharged from the ED with a history of a similar event, an event duration >1 minute, an abnormal medical history, and an altered responsiveness (P < .05). AAP risk criteria for all outcomes had a negative predictive value of 90% and a positive predictive value of 23%. CONCLUSIONS: AAP BRUE risk criteria are used to accurately identify patients at low risk for event recurrence, readmission, and a serious underlying diagnosis; however, their use results in the inaccurate identification of many patients as higher risk. This is likely because many AAP risk factors, such as age, are not associated with these outcomes.

Effects of participating in the annual Animal Welfare Assessment Contest on veterinary students' self-perceived knowledge of and attitudes toward animal welfare science and their career choices.

OBJECTIVE: To evaluate the impact of participating in the annual Animal Welfare Assessment Contest (AWJAC) on veterinary students' self-perceived knowledge of and attitudes toward animal welfare science and on participants' career choices. SAMPLE: 46 veterinary students who participated in the AWJAC from 2014 through 2017. PROCEDURES: The study consisted of 2 parts. In part 1, a survey regarding participation in the AWJAC was emailed to all 138 veterinary students who participated in the contest from 2014 through 2017. In part 2, a self-selected subset of 4 survey respondents were interviewed by telephone regarding their AWJAC experience. RESULTS: Forty-six of 138 (33%) AWJAC participants responded to the online survey. When respondents were asked to rate the attitudes they held before and after participating in the AWJAC, significant increases were identified for engaging with animal welfare topics in their professional decision-making, making career choices based on their interest in animal welfare, and having their interest in animal welfare shape their professional career choices. Analysis of telephone interview transcripts revealed 3 major themes regarding AWJAC participation: defining animal welfare, the value of networking, and professional preparedness. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that participation in the AWJAC heightened veterinary students' self-perceived awareness of animal welfare science, provided participants an opportunity to expand their professional networks, and prepared participants for entrance into the veterinary profession by enhancing communication and critical thinking skills.

The International Bathymetric Chart of the Arctic Ocean Version 4.0.

Bathymetry (seafloor depth), is a critical parameter providing the geospatial context for a multitude of marine scientific studies. Since 1997, the International Bathymetric Chart of the Arctic Ocean (IBCAO) has been the authoritative source of bathymetry for the Arctic Ocean. IBCAO has merged its efforts with the Nippon Foundation-GEBCO-Seabed 2030 Project, with the goal of mapping all of the oceans by 2030. Here we present the latest version (IBCAO Ver. 4.0), with more than twice the resolution (200 × 200 m versus 500 × 500 m) and with individual depth soundings constraining three times more area of the Arctic Ocean (∼19.8% versus 6.7%), than the previous IBCAO Ver. 3.0 released in 2012. Modern multibeam bathymetry comprises ∼14.3% in Ver. 4.0 compared to ∼5.4% in Ver. 3.0. Thus, the new IBCAO Ver. 4.0 has substantially more seafloor morphological information that offers new insights into a range of submarine features and processes; for example, the improved portrayal of Greenland fjords better serves predictive modelling of the fate of the Greenland Ice Sheet.

Beyond Suction-Feeding Fishes: Identifying New Approaches to Performance Integration During Prey Capture in Aquatic Vertebrates.

Organisms are composed of hierarchically arranged component parts that must work together to successfully achieve whole organism functions. In addition to integration among individual parts, some ecological demands require functional systems to work together in a type of inter-system performance integration. While performance can be measured by the ability to successfully accomplish ecologically relevant tasks, integration across performance traits can provide a deeper understanding of how these traits allow an organism to survive. The ability to move and the ability to consume food are essential to life, but during prey capture these two functions are typically integrated. Suction-feeding fishes have been used as a model of these interactions, but it is unclear how other ecologically relevant scenarios might reduce or change integration. To stimulate further research into these ideas, we highlight three contexts with the potential to result in changes in integration and underlying performance traits: (1) behavioral flexibility in aquatic feeding modes for capturing alternative prey types, (2) changes in the physical demands imposed by prey capture across environments, and (3) secondary adaptation for suction prey capture behaviors. These examples provide a broad scope of potential drivers of integration that are relevant to selection pressures experienced across vertebrate evolution. To demonstrate how these ideas can be applied and stimulate hypotheses, we provide observations from preliminary analyses of locally adapted populations of Trinidadian guppies (Poecilia reticulata) capturing prey using suction and biting feeding strategies and an Atlantic mudskipper (Periophthalmus barbarus) capturing prey above and below water. We also include a re-analysis of published data from two species of secondarily aquatic cetaceans, beluga whales (Delphinapterus leucas) and Pacific white-sided dolphins (Lagenorhynchus obliquidens), to examine the potential for secondary adaptation to affect integration in suction prey capture behaviors. Each of these examples support the broad importance of integration between locomotor and feeding performance but outline new ways that these relationships can be important when suction demands are reduced or altered. Future work in these areas will yield promising insights into vertebrate evolution and we hope to encourage further discussion on possible avenues of research on functional integration during prey capture.

Veterinarians and Humane Endings: When Is It the Right Time to Euthanize a Companion Animal?

Current advances in technologies and treatments provide pet owners and veterinarians with more options for prolonging the life of beloved pets, but can simultaneously lead to ethical dilemmas relating to what is best for both animal and owner. Key tools for improving end-of-life outcomes include (1) sufficient training to understand the valid ethical approaches to determining when euthanasia is appropriate, (2) regular training in client communication skills, and (3) a standard end-of-life protocol that includes the use of quality of life assessment tools, euthanasia consent forms, and pet owner resources for coping with the loss of a pet. Using these tools will improve outcomes for animals and their owners and reduce the heavy burden of stress and burnout currently being experienced by the veterinary profession.

Noise exposure, music, and animals in the laboratory: a commentary based on Laboratory Animal Refinement and Enrichment Forum (LAREF) discussions.

The effects of noise, in general, and music, in particular, on the behavior and welfare of animals in the laboratory deserve a great deal of empirical study. However, many laboratories must develop their current practices on the basis of sparse and conflicting data. With this commentary we seek to establish some of the factors that should be taken into account in deciding how to deal with sources of uncontrolled or deliberate sound and, specifically, in determining whether to play music in the laboratory. Views differ, however, the balance of evidence supports the use of quiet music during nonhuman animals' active periods, if this practice is introduced with an awareness of the risks to welfare and research.

Complex Systems Are More than the Sum of Their Parts: Using Integration to Understand Performance, Biomechanics, and Diversity.

Organisms are comprised of many interacting parts, and an increased number or specialization of those parts leads to greater complexity and the necessity for increased integration (the ability of those parts to perform together and maintain a functioning organism). Although this idea is widely recognized among biologists, organisms are more tangibly studied when those parts are considered independently. This reductionist approach has successfully advanced our understanding of organisms' performance. However, performance of one system might (or might not) be dependent on performance of another system to achieve a relevant outcome, and the mechanism of this dependence is poorly understood. We synthesize the concepts of complexity and integration and discuss their application in a biomechanical context. Capture of prey by predatory fishes is used as an example to highlight the application of these ideas. We provide a theoretical framework for future hypotheses of integration and predict an "integration space" for fishes that is then populated with data extracted from the literature. Additionally, using the kinematics of prey-capture in two species of sculpin (Scorpaeniformes: Cottidae), we show that species exhibit multivariate integration in distinct ways, and that these differences add additional insight into ecological divergence that would not be apparent by considering systems independently. Finally, we discuss new insights into organismal performance gained through the study of integration as an emergent property of kinematic systems working together during a common task. Integration is rarely the trait of interest, but we show that future work should adopt a more holistic approach to understand why and how animals perform complex behaviors.