RESUMEN
BACKGROUND: Image-based screens can produce hundreds of measured features for each of hundreds of millions of individual cells in a single experiment. RESULTS: Here, we describe CellProfiler Analyst, open-source software for the interactive exploration and analysis of multidimensional data, particularly data from high-throughput, image-based experiments. CONCLUSION: The system enables interactive data exploration for image-based screens and automated scoring of complex phenotypes that require combinations of multiple measured features per cell.
Asunto(s)
Células/ultraestructura , Biología Computacional/métodos , Procesamiento de Imagen Asistido por Computador/métodos , Fenotipo , Programas Informáticos , Inteligencia ArtificialRESUMEN
Biologists can now prepare and image thousands of samples per day using automation, enabling chemical screens and functional genomics (for example, using RNA interference). Here we describe the first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler. CellProfiler can address a variety of biological questions quantitatively, including standard assays (for example, cell count, size, per-cell protein levels) and complex morphological assays (for example, cell/organelle shape or subcellular patterns of DNA or protein staining).