New potential diagnostic markers for verrucous hyperplasia and verrucous carcinoma based on RNA-sequencing data.

Verrucous carcinoma (VC) is a rare subtype of squamous cell carcinoma (SCC) characterized by its histological presentation as a low-grade tumor with no potential for metastasis, setting it apart from invasive SCC. However, distinguishing VC from its benign counterpart, verrucous hyperplasia (VH), is challenging due to their clinical and morphological similarities. Despite the importance of accurate diagnosis for determining treatment strategies, diagnosis of VH and VC relied only on lesion recurrence after resection. To address this challenge, we generated RNA profiling data from tissue samples of VH and VC patients to identify novel diagnostic markers. We analyzed differentially expressed (DE) mRNA and long non-coding RNA (lncRNA) in tissue samples from VH and VC patients. Additionally, ChIP-X Enrichment Analysis 3 (ChEA3) was conducted to identify the top five transcription factors potentially regulating the expression of DE mRNAs in VH and VC. Our analysis of mRNA and lncRNA expression profiles in VH and VC provides insights into the underlying molecular characteristics of these diseases and offers potential new diagnostic markers. The identification of specific DE genes and lncRNAs may enable clinicians to more accurately differentiate between VH and VC, leading to better treatment choices.

Do Co-ethnic concentrated neighborhoods protect children with undocumented parents? Focusing on child behavioral functioning.

Undocumented immigrants concentrate in ethnic enclaves, but little is known about the implications of living in such neighborhoods for children of undocumented immigrants. Using data on Mexican-origin children from the Los Angeles Family and Neighborhood Survey and the decennial census, this paper examines the influence of co-ethnic concentrated neighborhoods on children's behavioral functioning, and the extent to which parental nativity and documentation status moderate the neighborhood effects. Multilevel linear regression models show that the proportion of co-ethnics in the neighborhood apparently has no influence on Mexican-origin children. However, cross-level interactions reveal that the neighborhood context has differential effects by parental documentation status. The higher the percentage of co-ethnics in the neighborhood, the lower (better) the externalizing scores for children of undocumented immigrants, a pattern not observed for children of US-born or documented parents. The mechanism through which co-ethnic enclaves benefit children of undocumented Mexican immigrants deserves future research.

Children of immigrants' behavioral trajectories and family processes: Using an integrative developmental model.

Using an integrative developmental model, this study revisited the "immigrant paradox" in early behavioral development, differentiating immigrant background from race and examining the role of family processes (family structure, stability, racial/ethnic socialization) in disparities between immigrant and U.S.-origin children. Growth-curve analyses on a sample of children aged 5-7 (N = 12,520) from the Early Childhood Longitudinal Study-Kindergarten class of 2010-2011 data set revealed distinct behavioral trajectories based on parental country of origin. Some groups outperformed the racially matched U.S.-origin peers by improving faster (internalizing behaviors: Salvadoran) or maintaining their advantages (internalizing: Mexican, Dominican; externalizing: Mexican, Salvadoran, Guatemalan, South American, and Dominican), while others faced continued challenges (externalizing behaviors: Japanese, Thai) or exhibited a nuanced pattern such as beginning with an advantage but deteriorating faster (internalizing: Cuban). The time-varying, country-of-origin-specific family processes fully or partly explained some disparities (except for Asian), emphasizing the need for a sociocultural understanding of the distinct strengths and limitations in racial-ethnic minority families. This contextualized knowledge is crucial for promoting healthy child development. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Spanking and executive functioning in US children: A longitudinal analysis on a matched sample.

BACKGROUND: Spanking has been linked to multiple maladaptive child outcomes. However, previous research linking spanking with children's executive function skills (EFs; inhibitory control, cognitive flexibility, and working memory) is limited by research designs that do not adequately address selection bias concerns, wherein the participant characteristics potentially differ between those who are spanked versus not spanked. OBJECTIVE: Using a representative sample of US children aged 5 to 6, this study strengthened the evidence for causal estimates on the link between spanking and subsequent EFs with a matched-group design. Low-frequency spanking and potential moderators (child gender, parent race/ethnicity, parental warmth) were tested to determine if they moderated the link between spanking and EFs. PARTICIPANTS: Data were drawn from the Early Childhood Longitudinal Study, Kindergarten Class of 2010-11 (Ns = 12,750-12,830). To mitigate selection bias, entropy-balanced matching was utilized to match spanked versus not-spanked groups, and lagged dependent variable regression analyses were conducted on the matched sample to predict EFs by spanking group status. RESULTS: After matching, spanking at age 5 was associated with lower inhibitory control and lower cognitive flexibility at age 6, but was not significantly predictive of later working memory. The association with inhibitory control was observed even for low frequency spanking. However, no evidence of moderation by child gender, parent race/ethnicity, and parental warmth was identified. CONCLUSIONS: This study provides evidence that spanking is associated with lower executive functioning in children, although the associations varied by different EF domains.

Sol-gel derived nanoporous compositions for entrapping small molecules and their outlook toward aptamer screening.

This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters "L", "U", "N", and "D". Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues.

Spanking and children's social competence: Evidence from a US kindergarten cohort study.

BACKGROUND: Spanking is a risk factor for children's social competency. However, establishing causality is a challenge, given selection bias in samples and the possibility of confounding the harms of excessive spanking with the effects of infrequent spanking. OBJECTIVE: This study addressed these causality issues to strengthen the causal estimates of the links between spanking and children's social competency. PARTICIPANTS AND SETTING: The study used longitudinal US kindergarten cohort data from children aged 5 to 7. METHODS: The study used matching and lagged dependent variables to mitigate selection bias associated with lifetime (Ns = 17,171-17,537) and recent (Ns = 10,393-10,724) incidence of spanking. Cases in which spanking frequency exceeded two times a week were excluded. Sample sizes are provided in ranges due to the variations across multiple imputed samples. RESULTS: Lifetime experience of spanking by age 5 was associated with higher externalizing behaviors at ages 6 and 7, and with lower self-control and interpersonal skills at age 6. A recent incidence of spanking at age 5 was associated with higher externalizing behaviors, lower self-control, and lower interpersonal skills at ages 6 and 7. These results remain significant after cases of frequent spanking were excluded. CONCLUSION: The results support the argument that spanking harms children's social development.

Extended kin and children's behavioral functioning: Family structure and parental immigrant status.

Using the Los Angeles Family and Neighborhood Survey (L.A. FANS), this paper examines the association between the presence of co-resident extended kin and children's internalizing and externalizing behaviors. The paper demonstrates the differential role of extended kin by family structure, as well as across parental immigrant status - specifically, nativity and documentation status. Children in the sample were found to be disadvantaged in extended family households, especially with regard to internalizing behaviors. This disadvantageous association was found mostly among married-parent extended family households, whereas there was no association between the presence of extended kin and behavior problems in children from single-parent families. This pattern emerged more clearly among children of documented immigrants, compared to those with native-born parents and those whose parents were unauthorized immigrants. These findings suggest a need to modify previous theories on extended family living arrangements; they also provide policy implications for immigrant families.

Sol-gel SELEX circumventing chemical conjugation of low molecular weight metabolites discovers aptamers selective to xanthine.

Sensitive detection of the metabolites indicative of a particular disease contributes to improved therapy outcomes. Developing binding reagents for detection of low molecular weight metabolites is hampered by the difficulty with immobilization of targets through appropriate covalent chemical linkage while ensuring that selected reagents retain specificity to unmodified metabolites. To circumvent chemical modification of targets, we employed sol-gel droplets deposited onto a porous silicon chip to entrap a purine metabolite, xanthine, which was found at lower levels in urine samples from patients with non-Hodgkin lymphoma. By sol-gel SELEX (systematic evolution of ligands by exponential enrichment) against xanthine, specific aptamers (KD ∼ 10 µM) with sensitivity of detection at as low as 1 µM were isolated, which bound to other purine metabolites at more than 100-fold lower affinity. In contrast, we failed to isolate xanthine-specific aptamers when SELEX was performed against xanthine covalently linked to polymer resin. This study demonstrates that the sol-gel platform for entrapping low molecular weight metabolites without chemical modifications can be utilized for SELEX to discover aptamers against clinical metabolite markers for diagnosis application.

A sol-gel-integrated protein array system for affinity analysis of aptamer-target protein interaction.

A sol-gel microarray system was developed for a protein interaction assay with high activity. Comparing to 2-dimensional microarray surfaces, sol-gel can offer a more dynamic and broad range for proteins. In the present study, this sol-gel-integrated protein array was used in binding affinity analysis for aptamers. Six RNA aptamers and their target protein, yeast TBP (TATA-binding protein), were used to evaluate this method. A TBP-containing sol-gel mixture was spotted using a dispensing workstation under high-humidity conditions and each Cy-3-labeled aptamer was incubated. The dissociation constants (K(d)) were calculated by plotting the fluorescent intensity of the bound aptamers as a function of the TBP concentrations. The K(d) value of the control aptamer was found to be 8 nM, which agrees well with the values obtained using the conventional method, electric mobility shift assay. The sol-gel-based binding affinity measurements fit well with conventional binding affinity measurements, suggesting their possible use as an alternative to the conventional method. In addition, aptamer affinity measurements by the sol-gel-integrated protein chip make it possible to develop a simple high-throughput affinity method for screening high-affinity aptamers.

Development of single-stranded DNA aptamers for specific Bisphenol a detection.

The development of reagents with high affinity and specificity to small molecules is crucial for the high-throughput detection of chemical compounds, such as toxicants or pollutants. Aptamers are short and single-stranded (ss) oligonucleotides able to recognize target molecules with high affinity. Here, we report the selection of ssDNA aptamers that bind to Bisphenol A (BPA), an environmental hormone. Using SELEX process, we isolated high affinity aptamers to BPA from a 10(15) random library of 60 mer ssDNAs. The selected aptamers bound specifically to BPA, but not to structurally similar molecules, such as Bisphenol B with one methyl group difference, or 4,4'-Bisphenol with 2 methyl groups difference. Using these aptamers, we developed an aptamer-based sol-gel biochip and detected BPA dissolved in water. This novel BPA aptamer-based detection can be further applied to the universal and high-specificity detection of small molecules.