[Unloading with insoles, orthotics and braces : Pre-operative leg axis correction or independent treatment?] / Unloading mit Einlagen, Orthesen und Federn : Vorstufe zur Achskorrektur oder eigenständige Therapie?

BACKGROUND: Knee osteoarthritis (OA) is a leading joint disease. In most of the early stages it does not involve the whole knee joint. Often, symptoms only or mainly concern the medial compartment combined with a slight varus malalignment. OBJECTIVES: Do valgus braces or laterally wedged insoles influence biomechanics and thus improve pain and function in patients with medial OA? Does the OA grade, severity of malalignment or patient's body weight predict the efficacy of the above-mentioned conservative treatment options? MATERIALS AND METHODS: The current literature was reviewed in regard to biomechanical changes to joint loading and their correlation to clinical results. RESULTS: Valgus braces and laterally wedged insoles reduce knee adduction moment, varus malalignment and analgesic consumption. Some authors suggest that mainly an alteration in muscle activity (diminished muscle co-contractions) is responsible for pain relief. Body weight and severity of varus malalignment did not influence treatment results; a significant correlation with OA severity was shown only for laterally wedged insoles. For both devices, compliance problems - especially long-term - should be considered, and conclusive evidence of positive clinical effects cannot be stated. CONCLUSIONS: Despite positive evidence in the current literature, a recommendation for or against valgus (unloader) braces in medial OA is not possible due to inconclusive results. Laterally wedged insoles are not recommended. Especially the long-term results are doubtful, and are possibly related to lacking compliance.

How fast is optically induced electron transfer in organic mixed valence systems?

The rate of thermally induced electron transfer in organic mixed valence compounds has thoroughly been investigated by e.g. temperature dependent ESR spectroscopy. However, almost nothing is known about the dynamics of optically induced electron transfer processes in such systems. Therefore, we investigated these processes in mixed valence compounds based on triphenylamine redox centres bridged by conjugated spacers by NIR transient absorption spectroscopy with fs-time resolution. These experiments revealed an internal conversion (IC) process to be on the order of 50-200 fs which is equivalent to the back electron transfer after optical excitation into the intervalence charge transfer band. This IC is followed by ultrafast cooling to the ground state within 1 ps. Thus, in the systems investigated optically induced electron transfer is about 3-4 orders of magnitude faster than thermally induced ET.

Two-dimensional vibronic spectroscopy of molecular aggregates: Trimers, dimers, and monomers.

The two-dimensional (2D) vibronic spectroscopy of molecular trimers is studied theoretically. The solution of the time-dependent Schrödinger equation is carried out with the multi-configurational time-dependent Hartree (MCTDH) method which allows for an efficient propagation of the multi-component wave functions. 2D-spectra are calculated for H- and J-type aggregates incorporating one or two vibrational modes for each monomer. In performing calculations for monomer, dimer, and trimer systems, it is documented how the vibronic structure of the 2D-spectrum changes upon aggregation. This is of importance for the characterization of aggregation behavior being influenced by experimental conditions such as temperature or concentration.

A new procalcitonin optical immunosensor for POCT applications.

A new immunosensor for the determination of procalcitonin was developed. A sandwich assay format was implemented on a polymethylmetacrylate optical biochip, opportunely shaped in order to obtain several flow channels and potentially suitable for point of care testing applications. The sandwich format makes use of two new rat monoclonal antibodies. The capture antibody was covalently immobilised on the surface of the plastic chip, and the detection antibody was labelled with DY647 dye. Different combinations of capture and detection antibodies were investigated, and particular attention was devoted in order to avoid the non-specific adsorption. A limit of detection of 0.088 mg L(-1) was achieved within the working range of 0.28-50 mg L(-1) in buffer samples. The assay was also implemented in human serum, and 0.2 and 0.7-25 mg L(-1) were the attained limit of detection and working range, respectively.