RESUMEN
Fly ash (FA) management is a key concern of ecologists around the world, so its potential as a nutritional supplement for agro-ecosystems needs to be explored. Therefore, alternate techniques that are eco-friendly to manage this emerging dual-edged waste are preferable in this field. The current study sought to determine the soil-modifying, crop yield improvement, and nematicidal properties of FA. In this study, beetroot seeds were sown in pots comprising field soil amended with differing proportions of FA (w/w) revealed the bio-fold properties of FA. Biomineralization and mapping of elements revealed that increased nutritional elements in soil supplemented with 15% FA induced growth-performance and yield of beetroot. Molecularly and morphologically characterized Meloidogyne incognita was used as nematode in this study for optimization of nematicidal properties FA. Plant growth performance, photosynthetic pigments, and yield of beetroot were significantly reduced owing to M. incognita as compared to control (un-treated and un-inoculated), and 15% FA reversed the negative effect of M. incognita significantly (P < 0.05) as compared to control plants. Confocal laser microscopy confirmed that 15% FA augmented in soil reduced nematode-juvenile invasion in beetroot as compared with control. The PCA (principal component analysis) accounted for 98.63% and 98.8% for the total-data variability in plants without nematodes and total data variability in treated plants (M. incognita + FA) respectively, which showed fit for a significant correlation between the various studied parameters in present study.
Asunto(s)
Beta vulgaris , Tylenchoidea , Animales , Ceniza del Carbón , Ecosistema , SueloRESUMEN
Endophytic fungi colonize interior plant tissue and mostly form mutualistic associations with their host plant. Plant-endophyte interaction is a complex mechanism and is currently a focus of research to understand the underlying mechanism of endophyte asymptomatic colonization, the process of evading plant immune response, modulation of gene expression, and establishment of a balanced mutualistic relationship. Fungal endophytes rely on plant hosts for nutrients, shelter, and transmission and improve the host plant's tolerance against biotic stresses, including -herbivores, nematodes, bacterial, fungal, viral, nematode, and other phytopathogens. Endophytic fungi have been reported to improve plant health by reducing and eradicating the harmful effect of phytopathogens through competition for space or nutrients, mycoparasitism, and through direct or indirect defense systems by producing secondary metabolites as well as by induced systemic resistance (ISR). Additionally, for efficient crop improvement, practicing them would be a fruitful step for a sustainable approach. This review article summarizes the current research progress in plant-endophyte interaction and the fungal endophyte mechanism to overcome host defense responses, their subsequent colonization, and the establishment of a balanced mutualistic interaction with host plants. This review also highlighted the potential of fungal endophytes in the amelioration of biotic stress. We have also discussed the relevance of various bioactive compounds possessing antimicrobial potential against a variety of agricultural pathogens. Furthermore, endophyte-mediated ISR is also emphasized.
Asunto(s)
Endófitos , Hongos , Endófitos/fisiología , Hongos/fisiología , Simbiosis , Plantas/microbiología , BacteriasRESUMEN
The sustainable management of large amounts of fly ash (FA) is a concern for researchers, and we aim to determine the FA application in plant development and nematicidal activity in the current study. A pot study is therefore performed to assess the effects of adding different, FA-concentrations to soil (w/w) on the infection of chickpea plants with the root-knot nematode Meloidogyne incognita. Sequence characteristic amplified region (SCAR) and internal transcribed spacer (ITS) region-based-markers were used to molecularly confirm M. incognita. With better plant growth and chickpea yield performance, FA enhanced the nutritious components of the soil. When compared with untreated, uninoculated control (UUC) plants, the inoculation of M. incognita dramatically reduced chickpea plant growth, yield biomass, and metabolism. The findings showed that the potential of FA to lessen the root-knot nematode illness in respect of galls, egg-masses, and reproductive attributes may be used to explain the mitigating effect of FA. Fascinatingly, compared with the untreated, inoculated control (UIC) plants, the FA treatment, primarily at 20%, considerably (p ≤ 0.05) boosted plant growth, yield biomass, and pigment content. Additionally, when the amounts of FA rose, the activity of antioxidants like superoxide dismutase-SOD, catalase-CAT, and peroxidase-POX as well as osmo-protectants like proline gradually increased. Therefore, our findings imply that 20% FA can be successfully applied as a potential strategy to increase biomass yield and plant growth while simultaneously reducing M. incognita infection in chickpea plants.
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Cicer , Ceniza del Carbón , Tylenchoidea , Animales , Tylenchoidea/fisiología , Raíces de Plantas/parasitología , Suelo/química , Suelo/parasitología , Enfermedades de las Plantas/parasitología , Enfermedades de las Plantas/prevención & controlRESUMEN
Root-knot nematodes (Meloidogyne spp.) are obligate sedentary endoparasites, considered severe crop-damaging taxa among all plant-parasitic nematodes globally. Their attacks through parasitic proteins alter the physiology and machinery of the host cells to favour parasitism and reduction in crop yield. Currently, the use of excessive pesticides as a fast remedy to manage this pest is hazardous for both the environment and humans. Keeping this view in mind, there is an urgent need for developing efficient eco-friendly strategies. Bio-control as an eco-friendly is considered the best approach to manage nematodes without disturbing non-target microbes. In bio-control, living agents such as fungi and bacteria are the natural enemies of nematodes and the best substitute for pesticides. Fungi, including nematode-trapping fungi, can sense host signals and produce special trapping devices viz., constricting rings and adhesive knobs/loops, to capture nematodes and kill them. Whereas, endo-parasitic fungi kill nematodes by enzymatic secretions and spore adhesion through their hyphae. Bacteria can also control nematodes by producing antibiotic compounds, competing for nutrients and rhizosphere, production of hydrolytic enzymes viz., chitinases, proteases, lipases, and induction of systemic resistance (ISR) in host plants. Scientists throughout the world are trying to evolve environmentally benign methods that sustain agricultural production and keep nematodes below a threshold level. Whatever methods evolve, in the future the focus should be on important aspects like green approaches for managing nematodes without disturbing human health and the environment.
RESUMEN
The potential of nanotechnology for the development of sustainable agriculture has been promising. The initiatives to meet the rising food needs of the rapidly growing world population are mainly powered by sustainable agriculture. Nanoparticles are used in agriculture due to their distinct physicochemical characteristics. The interaction of nanomaterials with soil components is strongly determined in terms of soil quality and plant growth. Numerous research has been carried out to investigate how nanoparticles affect the growth and development of plants. Nanotechnology has been applied to improve the quality and reduce post-harvest loss of agricultural products by extending their shelf life, particularly for fruits and vegetables. This review assesses the latest literature on nanotechnology, which is used as a nano-biofertilizer as seen in the agricultural field for high productivity and better growth of plants, an important source of balanced nutrition for the crop, seed germination, and quality enrichment. Additionally, post-harvest food processing and packaging can benefit greatly from the use of nanotechnology to cut down on food waste and contamination. It also critically discusses the mechanisms involved in nanoparticle absorption and translocation within the plants and the synthesis of green nanoparticles.
Asunto(s)
Alimentos , Eliminación de Residuos , Nanotecnología , Agricultura , Plantas , SueloRESUMEN
Alternative methods are needed to replace chemical nematicides because they have the potential to damage beneficial soil microbial diversity. Therefore, the present work was done to elucidate the soil ameliorative, plant-growth-promoting, and nematicidal properties of fly ash. A random block-designed pot experiment was conducted during the period, December 2018-February 2019. Seeds of carrot (Daucus carota L.) were sown under natural conditions in clay pots containing a growth medium comprising of field soil amended with different levels of fly ash. Plants were inoculated with Meloidogyne incognita that were molecularly characterized using 18S and D2/D3 fragments of 28S rDNA and morphologically through perineal pattern arrangement. The results revealed that fly ash application improved the soil's important physicochemical characteristics. The inoculation of M. incognita significantly reduced the plant growth, yield, and pigment content of carrot compared to the untreated uninoculated plants. Carrot grown in 15% fly ash (85:15 w/w field soil:fly ash) growth substrate had significantly (P ≤ 0.05) improved plant growth, yield, and pigment content as compared to the untreated inoculated plants. Moreover, the proline content and the activity of superoxide dismutase (SOD) and catalase (CAT) were enhanced by applying 15% fly ash. Fly ash amendment to the soil not only improved plant growth and yield but also reduced the gall index and egg mass index per root system of the carrot as well. Our results, therefore, suggest that 15% fly ash can be used in a sustainable way to improve the growth, yield, and resistance of carrot against the infection of M. incognita.