1.
J Comput Chem
; 41(14): 1330-1336, 2020 05 30.
Artículo
en Inglés
| MEDLINE
| ID: mdl-32067246
RESUMEN
Tri11 (now renamed as tri22) encoded cytochrome P450 monooxygenase in Trichoderma brevicompactum catalyzes the C-4 C-H hydroxylation of 12, 13-epoxytrichothec-9-ene (EPT) to produce trichodermol in the biosynthetic pathway of trichodermin/harzianum A. The density functional theory (DFT)-quantum mechanics (QM) approach is applied to elucidate the hydroxylation of EPT by using a model active species of P450 (Cpd I). The QM calculations were performed on the active site complex, to find out transition-state structure, intermediate, and product complexes for the two spin states at different potential energy surfaces. The two state reactivity rebound-free product formation resulted from the interplay of two spin states (doublet and quartet).