RESUMEN
PURPOSE: To describe various favorable courses of neuroblastoma (NBL) detected by mass screening and to present our observation program as a temporary treatment option, to be used until a final decision is made regarding the mass screening program for 6-month-old infants. PATIENTS AND METHODS: Between October 1993 and November 1999, 26 of 51 patients with NBL detected by mass screening were enrolled in our observation program. The criteria for observation included urinary vanillylmandelic acid (VMA) and homovanillic acid (HVA) levels less than 50 microg/mg creatinine, smaller tumor size (< 5.0 cm), preoperative status, and granted informed consent. Patients were divided into four groups according to changes in urinary VMA and HVA values and tumor size. Patients who no longer fulfilled criteria underwent surgery. RESULTS: The observation period ranged from 4 to 73 months. Urinary VMA and HVA levels decreased in 19 of 26 patients, often by age 16 months. Eighteen patients had regressing tumors, and in 10 of these cases, the tumor was undetectable or barely detectable by imaging techniques. Four patients younger than 12 months had increased tumor marker levels and tumor volume, histologically reflecting neuroblastic proliferation. The remaining three patients, all older than 18 months, had varied tumor marker levels but increased tumor volume, histologically reflecting an increase in Schwann cells. No upgrading of tumor stage or unfavorable biologic factor was noted in any patient. CONCLUSION: None of our patients showed evidence of transition from favorable to unfavorable prognosis, a finding that points to a reduction in the significance of screening as a public health measure. Until results of ongoing screening trials involving older patients have been evaluated, the observation program can be used as a temporary measure to avoid, with little risk, unnecessary surgical intervention.
Asunto(s)
Neoplasias Abdominales/diagnóstico , Neoplasias Abdominales/orina , Biomarcadores de Tumor , Tamizaje Masivo , Neuroblastoma/diagnóstico , Neuroblastoma/orina , Neoplasias Abdominales/cirugía , Neoplasias de las Glándulas Suprarrenales/diagnóstico , Neoplasias de las Glándulas Suprarrenales/cirugía , Neoplasias de las Glándulas Suprarrenales/orina , Femenino , Ácido Homovanílico/orina , Humanos , Lactante , Japón , Masculino , Neoplasias del Mediastino/diagnóstico , Neoplasias del Mediastino/cirugía , Neoplasias del Mediastino/orina , Regresión Neoplásica Espontánea , Neuroblastoma/cirugía , Neoplasias Pélvicas/diagnóstico , Neoplasias Pélvicas/cirugía , Neoplasias Pélvicas/orina , Evaluación de Programas y Proyectos de Salud , Estudios Prospectivos , Neoplasias Retroperitoneales/diagnóstico , Neoplasias Retroperitoneales/cirugía , Neoplasias Retroperitoneales/orina , Ácido Vanilmandélico/orinaRESUMEN
We report here a retrospective analysis of 36 children with therapy-related myelodysplastic syndrome (t-MDS) diagnosed between 1990 and 1999 in Japan. Their median age was 7.7 years and the median latency period for the development of t-MDS was 38.5 months. The primary tumors were hematologic in 15 of the cases and nonhematologic in 21. Chromosomal abnormalities were detected in 32/34(94%) patients: abnormalities of chromosomes 5and/or 7 in 41% and notably, 11q23 abnormalities in 31%. The prognosis of children with t-MDS was very poor as compared to children with primary MDS (5 year survival: 16% versus 54%, p<0.0001).
Asunto(s)
Terapia Combinada/efectos adversos , Síndromes Mielodisplásicos/inducido químicamente , Adolescente , Niño , Preescolar , Aberraciones Cromosómicas , Cromosomas Humanos Par 11/genética , Cromosomas Humanos Par 5/genética , Cromosomas Humanos Par 7/genética , Análisis Citogenético , Femenino , Humanos , Japón , Masculino , Síndromes Mielodisplásicos/diagnóstico , Síndromes Mielodisplásicos/genética , Pronóstico , Estudios Retrospectivos , Tamaño de la Muestra , Análisis de Supervivencia , Resultado del TratamientoRESUMEN
Spontaneous regression and maturation of neuroblastoma (NB) are well documented and occur frequently in infants, including those detected by mass screening. To seek histologic clues for regression/maturation in mass-screened NB, clinicopathologic features of 12 tumors that were resected after 2 to 18 months of untreated observation were reviewed. Unobserved screened and age-matched unscreened patients were also studied. To evaluate the possible important role of apoptosis, apoptotic cells were detected by in situ deoxyribonucleic acid (DNA) nick end labeling and immunohistochemical stain for activated caspase-3. Nests with a varying degree of reduced cellularity ("less cellular" and "hypocellular" nests) were common in patients younger than 18 months of age, and were rare in older patients. Two characteristic cells, which have not been focused previously, were frequent, especially in the hypocellular nests. One showed amorphic eosinophilic cytoplasm with pyknotic nuclei and the other contained plump cytoplasm with well-maintained nuclei. These cells were also observed in 89% of the unobserved screened NBs and 79% of the age-matched unscreened patients with good outcome, whereas they could not be confirmed in any of the age-matched unscreened NBs with poor outcome. The amorphic and plump cells were negative for activated caspase-3 and in situ DNA nick end labeling. From these results, the authors hypothesize that these cells most likely represent a degenerative process, in either a state before the activation of caspase-3 or a caspase-independent form of cell death. The presence of less cellular and hypocellular nests with amorphic/plump cells may serve as one of the important clues in predicting tumor prognosis.
Asunto(s)
Ganglioneuroma/patología , Neuroblastoma/patología , Factores de Edad , Apoptosis , Niño , Preescolar , Interpretación Estadística de Datos , Ganglioneuroblastoma/diagnóstico , Ganglioneuroblastoma/patología , Ganglioneuroblastoma/cirugía , Ganglioneuroma/diagnóstico , Ganglioneuroma/cirugía , Humanos , Inmunohistoquímica , Lactante , Tamizaje Masivo , Regresión Neoplásica Espontánea/patología , Neuroblastoma/diagnóstico , Neuroblastoma/cirugía , Pronóstico , Factores de RiesgoRESUMEN
We present an adrenal Ewing's sarcoma family of tumor (ESFT) arising in an 11-year-old Japanese boy. Although intensive chemoradiotherapy and radical surgery were performed, the patient died of obstinate disease 1 year and 3 months after the initial presentation. The primary site (adrenal gland) with radiologic findings (with foci of calcification), high titer of serum neuron specific enolase, and sheets of monotonous primitive rounded cells on histology mostly favored neuroblastoma. However, a diagnosis of ESFT was confirmed by immunohistochemical profile, including MIC2-positivity and molecular study disclosing EWS-FLI1 chimera gene verified by direct sequencing. Recognition of adrenal ESFT and use of newly developed diagnostic techniques are required for differential diagnosis of undifferentiated small round cell tumor of the adrenal gland.
Asunto(s)
Neoplasias de las Glándulas Suprarrenales/diagnóstico , Neoplasias Óseas/diagnóstico , Sarcoma de Ewing/diagnóstico , Antígeno 12E7 , Neoplasias de las Glándulas Suprarrenales/química , Neoplasias de las Glándulas Suprarrenales/genética , Neoplasias de las Glándulas Suprarrenales/ultraestructura , Antígenos CD/análisis , Biomarcadores de Tumor/análisis , Neoplasias Óseas/química , Neoplasias Óseas/genética , Neoplasias Óseas/ultraestructura , Calcinosis/patología , Moléculas de Adhesión Celular/análisis , Niño , ADN de Neoplasias/análisis , Diagnóstico Diferencial , Humanos , Técnicas para Inmunoenzimas , Masculino , Proteínas de Neoplasias/análisis , Neuroblastoma/diagnóstico , Proteínas de Fusión Oncogénica/genética , Fosfopiruvato Hidratasa/sangre , Proteína Proto-Oncogénica c-fli-1 , Proteína EWS de Unión a ARN , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sarcoma de Ewing/química , Sarcoma de Ewing/genética , Sarcoma de Ewing/ultraestructura , Factores de Transcripción/genéticaRESUMEN
We report the case of a patient with fatal obstructive lung disease after an HLA-haploidentical sibling cord blood transplant (CBT), with severe acute GVHD. A 2-year-old girl developed expiratory air trapping gradually with acute and chronic GVHD after CBT for the treatment of ALL. Anti-CMV and immunosuppressive therapy were ineffective, and the patient died of progressive respiratory acidosis. Necropsy of the lung revealed severe bronchiolitis obliterans with cytomegalic inclusion cells in the granulation tissues of the bronchiolitis. Thus, immunologic and GVHD problems can occur even in CBT.
Asunto(s)
Sangre Fetal , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Enfermedades Pulmonares Obstructivas/etiología , Bronquiolitis Obliterante/etiología , Bronquiolitis Obliterante/patología , Preescolar , Infecciones por Citomegalovirus/etiología , Infecciones por Citomegalovirus/patología , Resultado Fatal , Femenino , Enfermedad Injerto contra Huésped/etiología , Antígenos HLA , Haplotipos , Humanos , Enfermedades Pulmonares Obstructivas/patología , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapiaRESUMEN
Seventeen cases (age at onset, 1 month to 18 years; M/F, 9/8) of hemophagocytic syndrome which received allogeneic hematopoietic stem cell transplantation (SCT) in Japan during the period 1988-1998 are reported. The patients consisted of six familial inheritance-proven erythrophagocytic lymphohistiocytosis (FEL), five familial inheritance-unknown and infective agents-unknown HLH (of which two were highly likely to have been FEL with characteristic CNS signs), and six aggressive Epstein-Barr virus (EBV)-related HLH (of which two were natural killer cell-type large granular leukemia/lymphoma-associated hemophagocytic syndrome, EBV-NK-LGLL-HPS). All cases were treated intensively with immuno-chemotherapy, or with chemotherapy before SCT. As sources of SCT, 12 cases received bone marrow cells (sibling six, father one, URD five), two cord blood, two purified CD34-positive cells, and one PBSC. SCTs were successful in all 17 cases, apart from one receiving CD34-positive SCT. Following SCT, four patients relapsed and five died with a median follow-up of 23 months. Among the relapsed cases, the two EBV-NK-LGLL-HPS previously published as successfully transplanted were included. Among the fatal cases, three patients died from relapsed active disease and the remaining two from fatal post-SCT EBV-positive T cell lymphoma and extensive chronic GVHD, respectively. As of the end of September 1998, 10 patients are alive without disease for 3.5 months to 147 months, while two post-SCT patients are still having therapy for residual/recurrent disease. The Kaplan-Meier analysis showed a 2-year event-free survival after SCT as 54.0+/-13.0%.
Asunto(s)
Trasplante de Células Madre Hematopoyéticas , Histiocitosis de Células no Langerhans/terapia , Adolescente , Niño , Preescolar , Femenino , Histiocitosis de Células no Langerhans/epidemiología , Humanos , Lactante , Japón/epidemiología , Masculino , Factores de Tiempo , Donantes de TejidosRESUMEN
Cord blood stem cell transplantation (CBSCT) was performed on a patient with acute promyelocytic leukemia. The patient was a boy 3 years and 8 months old, who had shown complete remission following treatment with intensive chemotherapy. However, after the final course of consolidation chemotherapy, chromosome analysis of his bone marrow aspirates revealed 46XY, t(15,17)(q22;q21), and a PML-RAR alpha fusion gene was detected by the reverse transcriptase-polymerase chain reaction test. All-trans retinoic acid diminished the chromosomal abnormality, but the PML-RAR alpha fusion gene remained. The patient was then treated with CBSCT from an HLA-matched sibling donor. The number of nucleated cells in the cord blood was 2.2 x 10(7)/kg of body weight, and that of granulocyte-macrophage colony-forming units 0.6 x 10(4)/kg. Methotrexate was given, on days 3 and 6, as prophylaxis against graft-versus-host disease (GVHD). The neutrophil count rose to above 500/microliters on day 22. The platelet count exceeded 50,000/microliters on day 48. Platelet transfusions were given 12 times after CBSCT, the last one on day 36. Grade I acute GVHD was treated with steroids. The patient was well and discharged on day 103, without symptoms or laboratory data suggestive of relapse. Following this experience we instituted a project of the Kanagawa Cord Blood Bank, which is scheduled for expansion nationwide.
Asunto(s)
Sangre Fetal , Trasplante de Células Madre Hematopoyéticas , Leucemia Promielocítica Aguda/terapia , Adulto , Bancos de Sangre , Recuento de Células Sanguíneas , Preescolar , Femenino , Factor Estimulante de Colonias de Granulocitos/sangre , Prueba de Histocompatibilidad , Humanos , Recién Nacido , Japón , Leucemia Promielocítica Aguda/sangre , Leucemia Promielocítica Aguda/genética , Masculino , Núcleo Familiar , Proyectos Piloto , Embarazo , Trasplante HomólogoRESUMEN
P53 protein expression in malignant cells of five patients with Burkitt lymphoma (BL) and from two patients with B-cell acute lymphoblastic leukemia (B-ALL) was examined with anti p53 protein monoclonal antibodies PAb1801, PAb240 and p53-D07 using an immunocytochemical technique. Four of the seven patients were positive. The distribution of positive staining within the cell was predominantly in the nucleus. The reactivity of PAb240 was weaker than that of the other antibodies. In addition, three of the four positive cases showed the same abnormal karyotype; translocation (8;14) (q24;q32). All of the four positive cases died due to relapse of their primary disease. The three negative cases did not show karyotypic abnormalities and are still alive and well. In conclusion, p53 immunostaining technique may be useful for predicting the clinical outcome of B-cell malignancy.
Asunto(s)
Biomarcadores de Tumor , Linfoma de Burkitt/metabolismo , Proteína p53 Supresora de Tumor/metabolismo , Adolescente , Linfoma de Burkitt/genética , Linfoma de Burkitt/patología , Linfoma de Burkitt/fisiopatología , Niño , Cromosomas Humanos Par 14 , Cromosomas Humanos Par 8 , Femenino , Humanos , Inmunohistoquímica , Cariotipificación , Masculino , Pronóstico , Translocación Genética , Proteína p53 Supresora de Tumor/análisisRESUMEN
We report a rare pediatric case of aggressive natural killer (NK) cell lymphoma, characterized by acute onset hepatosplenomegaly and respiratory distress, and infiltration by large granular lymphocytes with the phenotype of CD3-CD16-CD56+. The patient has remained in complete remission after short-pulse intensive chemotherapy, and myeloablative therapy followed by allogeneic bone marrow transplantation. We compare our case with 7 other children with NK cell leukemia reported from other institutions.
Asunto(s)
Células Asesinas Naturales/inmunología , Células Asesinas Naturales/patología , Linfoma/patología , Linfoma/terapia , Adolescente , Antígenos de Superficie/metabolismo , Trasplante de Médula Ósea , Niño , Femenino , Fiebre , Enfermedad Injerto contra Huésped/tratamiento farmacológico , Herpesvirus Humano 4 , Humanos , Leucemia/patología , Hígado/patología , Linfoma/inmunología , Masculino , Inducción de Remisión , Bazo/patologíaRESUMEN
Neonatal alloimmune thrombocytopenic purpura (NAITP) is one of the causes of thrombocytopenia in the newborn period. The thrombocytopenia is caused by maternal transplacental antiplatelet alloantibodies. We report a case of NAITP in a newborn infant having subarachnoid hemorrhage. Examination of platelet antibodies revealed anti-Yukb, that is, human platelet antigen (HPA)-4a incompatibility. Cranial ultrasound and brain magnetic resonance imaging revealed subarachnoid hemorrhage in the temporal region inferior to the cephalohematoma. The lesion seemed to have been sustained during delivery. The patient was treated with high-dose gamma-globulin and several transfusions of random donor platelets and showed a good clinical course. This is the second reported case of NAITP associated with the Yuk antigen system having intracranial hemorrhage.
Asunto(s)
Antígenos de Plaqueta Humana/inmunología , Hemorragia Cerebral/etiología , Isoanticuerpos/inmunología , Púrpura Trombocitopénica/complicaciones , Púrpura Trombocitopénica/inmunología , Hemorragia Cerebral/diagnóstico por imagen , Hemorragia Cerebral/inmunología , Ecoencefalografía , Femenino , Pruebas de Inhibición de Hemaglutinación , Humanos , Recién Nacido , Integrina beta3 , Imagen por Resonancia Magnética , Linaje , Recuento de Plaquetas , Púrpura Trombocitopénica/sangreRESUMEN
The authors present unusual clinicopathologic findings of a patient with neuroblastoma stage 4S that recurred 11 years after induction of complete remission with chemotherapy. A 12-year-old girl presented with recurrent tumor in the liver. Urinary catecholamine metabolites were within normal range in contrast to the increased values at initial presentation. She underwent left lateral segmentectomy and biopsy of the right lobe. Histologic analysis of the recurrent tumor showed undifferentiated neuroblastoma intermingled with mature ganglioneuromatous lesions. There also were scattered ganglioneuromatous lesions throughout nontumorous area of the liver. Although multimodal intensified treatments including autologous bone marrow transplantation were performed, the patient died of obstinate recurrent tumor at age 14 years. The clinicopathologic findings suggested dedifferentiation from the ganglioneuromatous lesion rather than ordinary recurrence of the primary tumor. The current case and the literature review may indicate that long-term follow-up would be necessary for neuroblastoma stage 4/4S cases. J Pediatr Surg 36:953-955.
Asunto(s)
Neoplasias Hepáticas/secundario , Neoplasias Primarias Desconocidas/patología , Neuroblastoma/secundario , Niño , Terapia Combinada , Resultado Fatal , Femenino , Humanos , Neoplasias Hepáticas/patología , Neoplasias Hepáticas/terapia , Neoplasias Primarias Desconocidas/terapia , Neuroblastoma/patología , Neuroblastoma/terapia , RecurrenciaRESUMEN
BACKGROUND: Desmoplastic infantile ganglioglioma (DIG) is a rare intracranial tumor of infancy, characterized by solid and cystic components, voluminous size and supratentorial location. Although its histologic features have been reported, there has been no cytologic description of the tumor. Cytologic findings on imprint and aspirated material from two cases of histologically verified DIG are discussed. CASES: A 12-month-old male with cutis marmorata telangiectatica congenita and a 44-month-old female with episodes of spasm were referred to our center. Radiologic examination revealed a large, cystic, supratentorial mass in both patients. The mass was surgically removed, and histology revealed prominent desmoplasia with a mixture of astroglial and neuronal cells. Cytologic findings on imprint material and the needle aspirates taken from the cystic area of the tumor revealed a few isolated or sheetlike arrangements of small cells positive for glial fibrillary acidic protein and a few large cells with abundant cytoplasm and prominent nucleoli, positive for neuronal marker. CONCLUSION: Although distinction between ganglioglioma and DIG may be difficult by cytology alone, with the characteristic clinical presentation and radiologic findings, the possibility of DIG should be kept in mind when the specimens contain both astroglial and neuronal elements.
Asunto(s)
Neoplasias Encefálicas/patología , Encéfalo/patología , Ganglioglioma/patología , Biopsia con Aguja , Encéfalo/cirugía , Neoplasias Encefálicas/química , Neoplasias Encefálicas/cirugía , Preescolar , Femenino , Ganglioglioma/química , Ganglioglioma/cirugía , Proteína Ácida Fibrilar de la Glía/análisis , Humanos , Inmunohistoquímica , Lactante , Imagen por Resonancia Magnética , Masculino , Proteínas de Neurofilamentos/análisis , Sinaptofisina/análisisRESUMEN
In order to detect early-stage left ventricular dysfunction, we examined a load-independent index, that is, the stress-velocity relation, by echocardiography in 13 patients with aplastic anemia including pure red cell anemia. Even when left ventricular contractility and pump function were within the normal range, its afterload had a tendency to increase in transfusion-dependent patients. The patients who had impairment of cardiac pump function died of congestive heart failure within one year after abnormal findings in stress-velocity relation were detected. Therefore, the stress-velocity relation is a sensitive, useful and noninvasive index for detecting asymptomatic myocardial dysfunction in patients with transfusion-dependency and chronic iron overload. It is necessary for these patients to undergo examinations of echocardiography at regular intervals. In case of abnormally in the stress-velocity relation, treatment for afterload mismatch and more effective chelation should be recommended to reduce the burden on the heart. Thereby, the heart is kept in better condition and the prognosis of these patients will be improved.
Asunto(s)
Anemia Aplásica/complicaciones , Ecocardiografía , Sobrecarga de Hierro/etiología , Reacción a la Transfusión , Disfunción Ventricular Izquierda/diagnóstico , Disfunción Ventricular Izquierda/etiología , Adolescente , Niño , Preescolar , Enfermedad Crónica , Humanos , Quelantes del Hierro/uso terapéutico , Sobrecarga de Hierro/prevención & control , Sensibilidad y EspecificidadRESUMEN
Primary graft failure (pGF) is associated with considerable morbidity and mortality. Salvage hematopoietic SCT (HSCT) can rescue pGF patients; however, the optimal preconditioning regimen and stem cell source are yet to be determined, particularly in children. In this study, we retrospectively analyzed 102 pediatric patients who received salvage allogeneic HSCT for pGF. Salvage HSCT from matched or one-Ag-mismatched related donors (rMM01) provided superior OS compared with that from two- or three-Ags-mismatched related donors (rMM23) or cord blood transplantation (CBT). CBT showed a trend toward a slightly lower engraftment rate and late engraftment achievement compared with rMM23; however, the OS rate was similar between the two groups (47.6±7.7% for rMM23 and 45.7±8.6% for CBT, at 1 year after salvage HSCT). Multivariate analysis showed that preconditioning regimens with fludarabine or irradiation were associated with a higher engraftment rate and those with alkylating agents were associated with better OS. In conclusion, our results showed that rMM01 was the most suitable donor for salvage HSCT for pediatric pGF, and that CBT was an equally important option compared with rMM23 for patients without rMM01.
Asunto(s)
Rechazo de Injerto/cirugía , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Trasplante de Células Madre Hematopoyéticas/métodos , Terapia Recuperativa/métodos , Acondicionamiento Pretrasplante/métodos , Adolescente , Niño , Preescolar , Supervivencia de Injerto , Humanos , Lactante , Pronóstico , Factores de Riesgo , Tasa de Supervivencia , Trasplante Homólogo , Resultado del TratamientoRESUMEN
A second SCT is generally accepted as the only potentially curative approach for ALL patients that relapse after SCT, but the role of second SCT for pediatric ALL is not fully understood. We performed a retrospective analysis of 171 pediatric patients who received a second allo-SCT for relapsed ALL after allo-SCT. OS at 2 years was 29.4 ± 3.7%, the cumulative incidence of relapse was 44.1 ± 4.0% and non-relapse mortality was 18.8 ± 3.5%. Relapse occurred faster after the second SCT than after the first SCT (117 days vs 164 days, P=0.04). Younger age (9 years or less), late relapse (180 days or more after first SCT), CR at the second SCT, and myeloablative conditioning were found to be related to longer survival. Neither acute GVHD nor the type of donor influenced the outcome of second SCT. Multivariate analysis showed that younger age and late relapse were associated with better outcomes. Our analysis suggests that second SCT for relapsed pediatric ALL is an appropriate treatment option for patients that have achieved CR, which is associated with late relapse after the first SCT.
Asunto(s)
Trasplante de Células Madre Hematopoyéticas , Leucemia-Linfoma Linfoblástico de Células Precursoras/mortalidad , Leucemia-Linfoma Linfoblástico de Células Precursoras/prevención & control , Acondicionamiento Pretrasplante , Adolescente , Factores de Edad , Niño , Preescolar , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Recurrencia , Estudios Retrospectivos , Trasplante HomólogoRESUMEN
To assess the outcome of unrelated umbilical cord blood transplantation (UCBT), 141 children with AML who underwent UCBT (39 in first CR (CR1), 33 in CR2, 4 in CR3 and 65 at more advanced stages (not in CR)) were analyzed in a retrospective multicenter study in Japan. Short-term MTX was used for prophylaxis of acute GVHD in 80 cases (57%). The cumulative incidences of neutrophil recovery, platelet recovery and acute GVHD (grades 2-4) were 78.7, 62.4 and 40.1%, respectively, and the 100-day transplantation-related mortality (TRM) was 10.8%. Multivariate analysis showed that an infused CD34(+) cell dose of 1.35 x 10(5) cells per kg or more was associated with favorable neutrophil and platelet recovery, and that short-term MTX was associated with a lower 100-day TRM. The 6-year relapse rate was 38.8% and was associated with disease status. Six-year overall survival was 45.8% (70.4+/-8.3% in CR1, 59.3+/-11.3% in CR2, 75.5+/-21% in CR3 and 20.6+/-6.2% for children with non-CR). We conclude that the results of UCBT are particularly promising for children with a karyotype suggesting a poor prognosis, and for those who receive transplants in CR2 and CR3 after an early relapse.