Nicotine consumption may lead to aseptic loosening in proximal mega-prosthetic femoral replacement.

BACKGROUND: Aseptic loosening after total hip arthroplasty is likely related to nicotine ingestion. However, aseptic loosening as a direct consequence of smoking habits has not been described with regard to proximal mega-prosthetic femoral replacement. The aim of the present study was to evaluate the association between nicotine consumption and aseptic loosening rates after proximal mega-prosthetic femoral replacement. MATERIALS AND METHODS: A consecutive series of patients who received mega-prosthetic replacement of the proximal femur at our hospital between 2005 and 2015 were included. Their files were reviewed and evaluated for the influence of smoking on aseptic loosening rates. All living patients were invited to complete a functional follow-up assessment at our clinic. RESULTS: Twenty-six patients with 27 prostheses were included. Five patients were active smokers, and 21 patients were non-smokers. Aseptic loosening was observed in three patients in the smoking group, whereas none of the non-smokers developed aseptic loosening. Fisher's exact test showed a relationship between nicotine consumption and aseptic loosening of the prostheses (p = 0.003). CONCLUSIONS: Smoking increases the likelihood of aseptic loosening after proximal mega-prosthetic femoral replacement. LEVEL OF EVIDENCE: Level 4 according to Oxford Centre of Evidence-Based Medicine 2011.

Prognostic factors and therapeutic options for treatment of frozen shoulder: a systematic review.

PURPOSE: To evaluate the current status of scientific research on the natural history of frozen shoulder as published in the literature. MATERIALS AND METHODS: This systematic review was carried out on PubMed data and was guided by PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). Articles had to meet inclusion criteria. The quality of the papers was assessed using a newly developed tool, AMQPP (Assessing the Methodological Quality of Published Papers). The AMQPP score was correlated with the level of evidence rating according to the Oxford Centre for Evidence-Based Medicine. Suitable papers were divided into groups according to the shoulder condition on which they reported. This article focuses on the frozen shoulder. RESULTS: Seven articles on frozen shoulder met the inclusion criteria. One article was considered to have level 1 of evidence. Three articles had level 3 and the remaining 3 had level 4. Three papers assessed the natural history and the natural course of different forms of stiff shoulder. The others indirectly assessed the natural history by evaluating therapy trends. None of the articles clearly referred to the role of regression to the mean of frozen shoulder specifically. CONCLUSION: Spontaneous recovery to normal levels of function is possible and standardised non-operative treatment programmes are an effective alternative to surgery in most cases. However, patients with high risk factors such as diabetes mellitus, and those who suffer chronic symptoms or bilaterally affected, would benefit from early surgery. We also concluded that AMQPP score is simple and straight forward. It works as a quick quality-checking tool which helps researchers to identify the key points in each paper and reach a decision regarding the eligibility of the paper more easily. The AMQPP score is still open for further development.

Duration of sick leave after shoulder arthroscopy in Germany: analysis of health care data.

INTRODUCTION: The expected duration of incapacity after arthroscopic shoulder surgery is an important factor for therapy planning. The aim of this study was to analyze the duration of sick leave after arthroscopic shoulder surgery in Germany and to identify factors associated with a longer duration of sick leave. We hypothesized that certain patient-related factors may be associated with a longer duration of sick leave. METHODS: Routine health care data of all patients insured by one German health insurance company who underwent inpatient arthroscopic shoulder surgery between 2010 and 2012 were included in the analysis of the duration of sick leave in this retrospective cohort study (level III evidence). Comparisons were performed for different arthroscopic surgical procedures using the log-rank test. Possible factors that might be associated with a longer duration of incapacity were analyzed. RESULTS: Sick leave was reported in 303 of 660 cases. The median duration of sick leave was 82 days after subacromial decompression and 157 days after rotator cuff repair (p = 0.004). The duration of sick leave was longer in patients older than 50 years (p = 0.044) and in patients with sick leave that started prior to surgery (p < 0.001). Patients not receiving physiotherapy had a longer period of sick leave (p = 0.058). The median period of prescribed physiotherapy (64 days) was shorter than the period of incapacity. The duration of disability was not notably longer in female patients, patients taking opioid or antidepressant medication or diabetics. CONCLUSIONS: The duration of sick leave after arthroscopic shoulder surgery in Germany does not exceed sick leave duration in other countries. More complex procedures, advanced age and sick leave beginning prior to surgery resulted in longer durations of sick leave. Preoperative prognosis of the necessary duration of postoperative sick leave may not always be realistic. An extended period of sick leave can be expected after more complex procedures and in patients older than 50 years of age. Future studies should investigate whether a longer period of physiotherapy may help to shorten the period of postoperative sick leave.

The natural history of rotator cuff tears: a systematic review.

PURPOSE: To analyse the current scientific evidence regarding the natural history of the clinical and anatomical progression of rotator cuff tears. METHODS: A broad systematic review of the literature (PubMed database through January 2014) which was guided, conducted and reported according to PRISMA criteria. This article focuses on the rotator cuff tears. Articles had to meet an inclusion criteria. The methodological quality of each study was individually assessed using a recently developed general assessment tool AMQPP (assessing the methodological quality of published papers). RESULTS: Seven articles dealing with rotator cuff tears were included, one of them was a high-quality study. Three papers assessed the natural history and the natural course of rotator cuff rupture directly. The other studies indirectly assessed the natural history with reports on non-operative and operative therapy trends. All of these articles had been published in four different top medical journals according to 2013 ranking. We found no articles which clearly referred to the role of regression to the mean of rotator cuff tears. CONCLUSION: The development of symptoms and anatomical deterioration are often directly correlated. Spontaneous recovery to normal levels of function has been successfully achieved, and standardised non-operative treatment programmes are an effective alternative to surgery for many patients. Follow-up is necessary to avoid irreparable stage. However, surgery is still favoured by young active people and highly professional persons who need to get fit in a short period of time. Further research is still necessary. The AMQPP score system is simple and reliable. It works as a quick quality-checking tool which helps researchers to identify the key points in each paper and reach a decision regarding the eligibility of the paper more easily.

The impact of a high tibial valgus osteotomy and unicondylar medial arthroplasty on the treatment for knee osteoarthritis: a meta-analysis.

PURPOSE: Both high tibial valgus osteotomy (HTO) and unicompartmental medial knee arthroplasty (UKA) are established methods for the treatment for moderate stages of OA. This is the first global meta-analysis to compare the long-term effects of both methods regarding survival, outcomes and complications of total arthroplasty. METHODS: Literature research was performed using established medical databases: MEDLINE (via PubMed), EMBASE (via OVID) and the Cochrane register. Criteria for inclusion were as follows: English or German papers, a clinical trial with a clear description of survival, an outcome evaluation using a well-described knee score and a follow-up >5 years. Statistical analysis was performed using the special meta-analysis software called "Comprehensive Meta Analysis" (version 2.0; Biostat, Englewood, NJ, USA). RESULTS: Final meta-analysis after the full-text review included 46 studies about valgus HTO and 43 studies about medial UKA. There were no significant differences between valgus HTO and medial UKA in terms of the number of total required replacements. After a 5- to 8-year follow-up, 91.0% of the valgus HTO patients and 91.5% of medial UKA patients did not need a total replacement. This value was 84.4% for valgus HTOs and 86.9% for medial UKAs after a 9- to 12-year follow-up. Mean survival time to TKA was 9.7 years after valgus HTO and 9.2 years after medial UKA. Clinical outcome was significantly better after medial UKA in a 5- to 12-year follow-up. After more than 12 years, results were comparable in both groups. No significant differences were seen in the complication rates. CONCLUSIONS: This meta-analysis aimed to find the advantages and disadvantages of two established methods for the treatment for medial compartment knee osteoarthritis. Valgus HTO is more appropriate for younger patients who accept a slight decrease in their physical activity. Medial UKA is appropriate for older patients obtaining sufficient pain relief but with reduced physical activity. LEVEL OF EVIDENCE: II.

Biomechanical and magnetic resonance imaging evaluation of a single- and double-row rotator cuff repair in an in vivo sheep model.

PURPOSE: To investigate the biomechanical and magnetic resonance imaging (MRI)-derived morphologic changes between single- and double-row rotator cuff repair at different time points after fixation. METHODS: Eighteen mature female sheep were randomly assigned to either a single-row treatment group using arthroscopic Mason-Allen stitches or a double-row treatment group using a combination of arthroscopic Mason-Allen and mattress stitches. Each group was analyzed at 1 of 3 survival points (6 weeks, 12 weeks, and 26 weeks). We evaluated the integrity of the cuff repair using MRI and biomechanical properties using a mechanical testing machine. RESULTS: The mean load to failure was significantly higher in the double-row group compared with the single-row group at 6 and 12 weeks (P = .018 and P = .002, respectively). At 26 weeks, the differences were not statistically significant (P = .080). However, the double-row group achieved a mean load to failure similar to that of a healthy infraspinatus tendon, whereas the single-row group reached only 70% of the load of a healthy infraspinatus tendon. No significant morphologic differences were observed based on the MRI results. CONCLUSIONS: This study confirms that in an acute repair model, double-row repair may enhance the speed of mechanical recovery of the tendon-bone complex when compared with single-row repair in the early postoperative period. CLINICAL RELEVANCE: Double-row rotator cuff repair enables higher mechanical strength that is especially sustained during the early recovery period and may therefore improve clinical outcome.

Surgical management of sternoclavicular septic arthritis.

INTRODUCTION: Septic arthritis of the sternoclavicular joint (SCJ) is a rare condition and has many diagnostic and therapeutic standards. The purpose of this study was to evaluate our experience with surgical and diagnostic management to provide a surgical pathway to help surgeons treat this disease. METHOD: We retrospectively reviewed five patients who were managed surgically between 1999 and 2007. All patients underwent structured diagnostic and treatment protocols. The functional outcome was evaluated using the Constant Score. PATIENTS: The patients had the following underlying medical conditions: laryngeal cancer, port-explantation linked to a rectum carcinoma, spondylodiscitis, and brain stem infarct with reduced general condition; one patient had no underlying medical problems. Three patients underwent a simple incision, debridement and drainage, and two patients underwent an extended intervention with partial resection of the sternoclavicular joint. The mean duration of follow-up was 29 months (range 24-36 months). All patients had well-healed wounds without signs of reinfection. The Constant Score for the functional outcome at the time of the last follow-up was 76 points (range 67-93 points). All patients recovered completely from SCJ disease. CONCLUSION: Our recommendations for the management of septic arthritis of the sternoclavicular joint include standard treatment steps and assessments. The early stages of infection can be managed by simple incision, debridement and drainage. In advanced stages of infection, a more radical intervention is preferable.

Reliability in arthroscopic grading of cartilage lesions: results of a prospective blinded study for evaluation of inter-observer reliability.

INTRODUCTION AND AIM: Arthroscopy is a well-established method for grading cartilage lesions. This study was undertaken to evaluate the inter-observer variance of grading cartilage lesions in a real life operation. MATERIALS AND METHODS: Four experienced arthroscopic surgeons used diagnostic arthroscopy, one after the other, to grade cartilage lesions in a total of 16 patients who had undergone knee arthroscopy. RESULTS: In summary, a total of 14 cartilage areas in 16 patients were graded (n = 224). The Cohens (Fleiss) Kappa Index for multiple investigators was κ = 0.052 in the medial, κ = 0.300 in the central, and κ = 0.107 in the lateral surface of the patella. The indices were κ = 0.292 in the medial, κ = 0.0.255 in the central, and κ = 0.234 in the lateral surface of the trochlea. The inter-observer variance was κ = 0.193 in the MFC mean bearing zone, κ = 0.116 in the margin of the MFC, κ = 0.168 in the mean bearing zone of the TM, and κ = 0.164 in the TM margin. In the lateral compartment, the κ-Index was 0.309 in the LFC mean bearing zone, 0.111 in the margin of the LFC, 0.020 in the mean bearing zone of the TL, and 0.085 in the TL margin. CONCLUSIONS: The inter-observer reliability of the arthroscopic grading of cartilage lesions is poor. The major problem is the relatively large variability in differentiating between intact cartilage and lesions that consist of the softening of the cartilage and the differentiation between superficial and deep cartilage lesions. In the future, objective measurements should be developed to solve this problem.

Four-year results from a randomized controlled study of knee chondroplasty with concomitant medial meniscectomy: mechanical debridement versus radiofrequency chondroplasty.

PURPOSE: This randomized study was undertaken to compare the effectiveness of simple mechanical debridement and 50 degrees C controlled bipolar chondroplasty. METHODS: A total of 60 patients who had a grade III articular cartilage defect of the medial femoral condyle were included. After randomization, 30 patients underwent simple debridement of articular cartilage defects, which was performed with a mechanical shaver (mechanical shaver debridement [MSD] group). The remaining patients underwent thermal chondroplasty, which was performed with a temperature-controlled bipolar device with a constant thermo-application of a maximum of 50 degrees C (radiofrequency-based chondroplasty [RFC] group). All patients underwent partial (n = 41) or subtotal (n = 19) meniscectomy. Follow-up was undertaken 4 years postoperatively. RESULTS: No significant differences between the preoperative findings for the 2 groups were observed. One patient from the MSD group had died, and one female patient in the RFC group was lost to follow-up. A total of 18 patients had undergone revision operations for persistent knee problems: in the MSD group, there were 8 endoprostheses, 4 osteotomies, and 2 revision arthroscopies, whereas in the RFC group, there was 1 replacement, 2 osteotomies, and 1 revision arthroscopy with subtotal medial meniscectomy. The proportion of revisions was significantly higher in the MSD group (P = .006). These patients were excluded from the evaluation. The remaining 40 patients from both groups benefited from the operation. The preoperative Knee Injury and Osteoarthritis Outcome Score (KOOS) was 11.3 points in the MSD group and 15.5 points in the RFC group (P = .279). Patients from the MSD group had a KOOS of 53.2 points at the time of follow-up. In the RFC group the KOOS (71.8 points) was significantly higher (P < .001). CONCLUSIONS: Compared with classical mechanical debridement, bipolar radiofrequency currently appears to be the superior method for achieving a good midterm result. LEVEL OF EVIDENCE: Level I, randomized controlled trial.

Tendon-bone contact pressure and biomechanical evaluation of a modified suture-bridge technique for rotator cuff repair.

The aim of the study was to evaluate the time-zero mechanical and footprint properties of a suture-bridge technique for rotator cuff repair in an animal model. Thirty fresh-frozen sheep shoulders were randomly assigned among three investigation groups: (1) cyclic loading, (2) load-to-failure testing, and (3) tendon-bone interface contact pressure measurement. Shoulders were cyclically loaded from 10 to 180 N and displacement to gap formation of 5- and 10-mm at the repair site. Cycles to failure were determined. Additionally, the ultimate tensile strength and stiffness were verified along with the mode of failure. The average contact pressure and pressure pattern were investigated using a pressure-sensitive film system. All of the specimens resisted against 3,000 cycles and none of them reached a gap formation of 10 mm. The number of cycles to 5-mm gap formation was 2,884.5 + or - 96.8 cycles. The ultimate tensile strength was 565.8 + or - 17.8 N and stiffness was 173.7 + or - 9.9 N/mm. The entire specimen presented a unique mode of failure as it is well known in using high strength sutures by pulling them through the tendon. We observed a mean contact pressure of 1.19 + or - 0.03 MPa, applied on the footprint area. The fundamental results of our study support the use of a suture-bridge technique for optimising the conditions of the healing biology of a reconstructed rotator cuff tendon. Nevertheless, an individual estimation has to be done if using the suture-bridge technique clinically. Further investigation is necessary to evaluate the cell biological healing process in order to achieve further sufficient advancements in rotator cuff repair.

Fatigue stress fractures of the pelvis: a rare cause of low back pain in female athletes.

Stress fracture of the pelvis represents one rare differential diagnosis among the manifold causes of low back pain in female athletes. We report a case of fatigue stress fracture of the pelvis in a 24-year-old female athlete as an unusual differential diagnosis of low back pain that should be taken into consideration. According to the literature the incidence of low back pain in athletes ranges from 1% to 30% and is influenced by sport type, gender, training intensity, training frequency and technique. In some cases, no specific pain generator is found, which makes diagnosis and treatment difficult. The frequency of the injury and the treatment options are discussed, based on published studies.

Septic arthritis of the shoulder joint: an analysis of management and outcome.

We retrospectively reviewed the records of 21 patients (23 shoulders) who underwent surgical treatment for septic arthritis of the shoulder joint, between 2000 and 2007. Patients were on average 63.7 (41-85) years old; they were treated either by arthroscopic debridement (12 shoulders) or by combined arthroscopic and open procedures (11 shoulders). The mean duration of symptoms prior to surgery was 16 (5-76) days. The mean Constant score recorded at the last follow-up - on average 353 months (25-43) after surgery - was 73 (46-82) points. Patients with symptoms for two weeks or less prior to surgery had better results and a lower re-operation rate than those with symptoms longer than two weeks. Early infection can be managed arthroscopically, and satisfactory results can be expected. In advanced infection, a more radical approach is more appropriate.

Evaluation of cartilage defects with near-infrared spectroscopy (NIR): an ex vivo study.

Damaged cartilage undergoes complex changes in composition, histological structure, and mechanical properties. Near-infrared-spectroscopy (NIR spectroscopy) is an important method to measure changes in composition of complex composites. The present study was aimed at evaluating NIR spectroscopy as a means to quantitate tissue alterations in low grade cartilage defects. Fresh medial femoral condyles from female sheep were collected. Cartilage defects were graded according to the International Cartilage Repair Society (ICRS). Specimens were examined by a NIR spectroscopy device (spectral range of 1100-1700 nm). Absorption spectra were calculated from the reference and measurement spectra. As indicator for cartilage composition the ratio (absorption ratio, AR) of the two main absorption bands in this region was calculated. Mechanical stiffness was measured as Shore A. Water-, glycosaminoglycan-, and collagen content and histological grade (Mankin score) were determined. The NIR absorption in ICRS grade 1 defects (AR=2.1+/-0.1) was significantly higher than in intact cartilage (AR=1.5+/-0.1). ICRS grade 2 specimens tended to a higher NIR absorption. NIR absorption correlated significantly with Mankin score (R=0.896), water content (R=0.845), and mechanical stiffness (R=0.877). Initial cartilage degeneration correlates with changes in NIR absorption, indicating NIR spectroscopy to reflect complex structural changes in degenerated cartilage. The data suggest that NIR spectroscopy could be useful for in situ detection of early cartilage defects.

Biomechanical evaluation of rotator cuff repairs in a sheep model: suture anchors using arthroscopic Mason-Allen stitches compared with transosseous sutures using traditional modified Mason-Allen stitches.

BACKGROUND: The optimal method for rotator cuff repair of the shoulder is not yet known. The aim of this study was to compare the time-dependent biomechanical properties of the traditional open transosseous suture technique and modified Mason-Allen stitches (group 1) versus the double-loaded suture anchors technique and so-called arthroscopic Mason-Allen stitches (group 2) in rotator cuff repair. METHODS: Eighteen adult female sheep were randomized into two groups: in an open approach in which the released infraspinatus tendon was repaired with group 1, and with group 2. Animals were sacrificed at 6, 12, or 26 weeks; shoulders were harvested and magnetic resonance imaging was performed. Eight untreated contralateral shoulders served as controls. Tendons of 16 additional unpaired cadaver shoulder joints of adult female sheep were identically treated for analysis at time zero. In a biomechanical evaluation all specimens were loaded to failure at a constant displacement rate using a standard universal testing machine. The load-to-failure and stiffness of the healed bone-tendon interface were calculated. RESULTS: Magnetic resonance imaging analysis showed cuff integrity in all cases, and no evidence of foreign body reaction to the anchors. Load-to-failure and stiffness data did not indicate any significant difference between the two treatment groups, neither at 6 weeks nor at 12 or 26 weeks. However, at time zero the group 2 had a higher load-to-failure in comparison to the group 1 (P<0.010), but there was no difference for the stiffness (P<0.121). CONCLUSIONS: This in vivo study showed that, postoperatively, the group 2 technique provides superior stability and after healing would gain strength comparable to the group 1 technique.

The surgical technique of autologous chondrocyte transplantation of the talus with use of a periosteal graft. Surgical technique.

BACKGROUND: Despite its highly specialized nature, articular cartilage has a poor reparative capability. Treatment of symptomatic osteochondral defects of the talus has been especially difficult until now. METHODS: We performed autologous chondrocyte transplantation in twelve patients with a focal deep cartilage lesion of the talus. There were seven female and five male patients with a mean age of 29.7 years. The mean size of the lesion was 2.3 cm(2). All patients were studied prospectively. Evaluation was performed with use of the Hannover ankle rating score, the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score, a visual analog scale for pain, and magnetic resonance imaging. RESULTS: All patients were available for follow-up at a mean of sixty-three months. There was a significant improvement in the Hannover score, from 40.4 points preoperatively to 85.5 points at the follow-up examination, with seven excellent results, four good results, and one satisfactory result. The AOFAS mean score was 88.4 points compared with 43.5 points preoperatively. Magnetic resonance imaging showed a nearly congruent joint surface in seven patients, discrete irregularities in four, and an incongruent surface in one. The patients who had been involved in competitive sports were able to return to their full activity level. CONCLUSIONS: The promising clinical results of this study suggest that autologous chondrocyte transplantation is an effective and safe way to treat symptomatic osteochondral defects of the talus in appropriately selected patients.

Biomechanical comparison of double-loaded suture anchors using arthroscopic Mason-Allen stitches versus traditional transosseous suture technique and modified Mason-Allen stitches for rotator cuff repair.

BACKGROUND: In recent studies objective evaluations have demonstrated that arthroscopic rotator cuff repairs can have higher failure rates than open repairs. Thus, there is a need for a stronger tissue-holding stitch for arthroscopical repair. The purpose of this study was to compare the biomechanical properties of traditional open transosseous suture technique and modified Mason-Allen stitches versus double-loaded suture anchors and arthroscopic Mason-Allen stitches in rotator cuff repair. METHODS: In 20 sheep shoulders the infraspinatus tendons were dissected from their insertion and were randomized to 2 repair groups: (1) repair with transosseous suture and modified Mason-Allen stitches, (2) repair with double loaded bioabsorbable suture anchors and arthroscopic Mason-Allen stitches: Both groups were coupled with braided, nonabsorbable polyester (Ethibond) suture sized USP No. 2. All repairs were cyclically loaded from 10 to 180N with the use of a materials testing machine. The number of cycles to gap formation of 5 and 10mm at the repair site and the mode of failure were recorded. RESULTS: The number of cycles to 5-mm gap was mean 634 (SD 106) for group 1 and mean 750 (SD 107) for group 2 (P<0.026). The corresponding values to 10-mm gap were mean 1573 (SD 161) for group 1, and mean 1789 (SD 183 cycles) for group 2 (P<0.012). In group 2 the mode of failure occurred by tissue pull-out, whereas in group 1 the failure occurred by a mixture of suture breakage and pull-out. CONCLUSIONS: This time-zero study demonstrates that the combination of bioabsorbable suture anchors and arthroscopic Mason-Allen stitches provides strength superior to that of the modified Mason-Allen transosseous suture technique under isometric cyclic loading conditions. However, additional evaluation is needed to examine the effects on the sustained strength of the repair throughout the healing process.

Mechanical behavior of intact and low-grade degenerated cartilage.

Young's modulus, elastic and plastic deformation, mechanical hardness and load at failure were determined for low-grade degenerated hyaline cartilage in a porcine model. Osteochondral plugs from the medial condyle of 30 female pigs were used. Cartilage defects were classified using the International Cartilage Repair Society (ICRS) protocol. Mechanical hardness was measured using a Shore A testing device. Total stiffness and plastic deformation was evaluated in the range 50-200 N using a 5-mm indenter. The load at failure was then determined. ICRS grade I specimens showed significantly lower stiffness than grade 0 specimens. ICRS grade 0 specimen showed no significant plastic deformation within the load range 25-100 N. In degenerated cartilage, plastic deformation started at a significantly lower load (50 N). The Young's modulus at 25 N in ICRS grade 0 specimens (18.8 MPa) was significantly higher than in grade I (11.1 MPa) or grade II (10.5 MPa) specimens. Intact cartilage showed significantly higher tension at failure and mechanical Shore A hardness. Young's modulus and tension at failure showed strong correlation. Cartilage degeneration is associated with a significant loss of elasticity and mechanical stress resistance. Shore hardness measurement is an adequate method for rapid biomechanical evaluation of cartilage specimens.

Karl Fischer titration and coulometry for measurement of water content in small cartilage specimens.

This study evaluated the efficiency of Karl Fischer titration and coulometry for measurement of water content in small intact and defective cartilage specimens. Cartilage from the main weight-bearing zone of the medial condyle of 38 fresh sheep knees was used. Of these, 20 condyles had an intact cartilage, while defects (14 grade I and 4 grade II) were found in the rest. The mechanical hardness was determined as Shore A. Cartilage specimens of approximately 5 mg were analyzed in special devices for moisture measurement and then continuously heated up to 105 degrees C. The actual measurement was performed in an electric cell (coulometry). An electrode was laminated with hygroscopic phosphorus pentoxide. In the electrochemical reaction, H and O are liberated from the electrode. The requirement for electric energy correlates with the amount of water in the specimen. The water content in intact cartilage was 66.9%. Grade I (72.6%) and grade II (77.8%) defects had significantly higher water content. Significantly higher and faster spontaneous evaporation was observed in cartilage defects at room temperature. The water content and spontaneous water evaporation correlated with significantly lower mechanical hardness. The experimental design (combined method of thermogravimetry, Karl Fischer titration, and coulometry) was sufficient for evaluating the water content in small cartilage specimens. It is also possible to measure the temperature-dependent water liberation from cartilage specimens.