Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 31
Filtrar
Más filtros

Banco de datos
País/Región como asunto
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Int J Mol Sci ; 23(19)2022 Sep 29.
Artículo en Inglés | MEDLINE | ID: mdl-36232820

RESUMEN

Pancreatic cancer is one of the cancers with very poor prognosis; there is an urgent need to identify novel biomarkers to improve its clinical outcomes. Circulating tumor DNA (ctDNA) from liquid biopsy has arisen as a promising biomarker for cancer detection and surveillance. However, it is known that the ctDNA detection rate in resected pancreatic cancer is low compared with other types of cancer. In this study, we collected paired tumor and plasma samples from 145 pancreatic cancer patients. Plasma samples were collected from 71 patients of treatment-naïve status and from 74 patients after neoadjuvant therapy (NAT). Genomic profiling of tumor DNA and plasma samples was conducted using targeted next-generation sequencing (NGS). Somatic mutations were detected in 85% (123/145) of tumors. ctDNA was detected in 39% (28/71) and 31% (23/74) of treatment-naïve and after-NAT groups, respectively, without referring to the information of tumor profiles. With a tumor-informed approach (TIA), ctDNA detection rate improved to 56% (40/71) and 36% (27/74) in treatment-naïve and after-NAT groups, respectively, with the detection rate significantly improved (p = 0.0165) among the treatment-naïve group compared to the after-NAT group. Cases who had detectable plasma ctDNA concordant to the corresponding tumor showed significantly shorter recurrence-free survival (RFS) (p = 0.0010). We demonstrated that TIA improves ctDNA detection rate in pancreatic cancer, and that ctDNA could be a potential prognostic biomarker for recurrence risk prediction.


Asunto(s)
ADN Tumoral Circulante , Neoplasias Pancreáticas , Biomarcadores de Tumor/genética , ADN Tumoral Circulante/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Mutación , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/genética , Neoplasias Pancreáticas/cirugía , Neoplasias Pancreáticas
2.
Hepatol Res ; 46(4): 354-6, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26011571

RESUMEN

We present the case of a chronic hepatitis C (CHC) patient who was originally diagnosed with genotype 2a on serogroup 1 CHC genotype testing, but who was subsequently confirmed to have genotype 1b when using the hepatitis C virus (HCV) monitor genotype assay. The genotype 2a diagnosis was attributed to the fact that the type 2a-specific primer used in Okamoto et al.'s method (HCV genotype primer kit) has relatively high homology, which caused the amplification reaction to proceed, rendering a HCV RNA genotype test result of 2a. Genotype testing is important in determining whether a patient is indicated for concomitant dual oral therapy; however, the potential for different diagnoses such as described in this report highlights the importance of serogroup confirmation.

3.
Biol Pharm Bull ; 35(9): 1487-95, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22975499

RESUMEN

The incidence of hypersensitivity reaction (HR) to carboplatin has been reported to increase after repeated use of the drug. However, a reliable ex vivo test to predict HR to carboplatin is not currently available. We evaluated the clinical usefulness of measuring basophil CD203c to predict carboplatin-related HR in this prospective case-control study conducted at Mie University Hospital between October 2009 and September 2010. Eleven patients had history of carboplatin-related HR within the past 3 years, and 19 had no history of HR after receiving more than 5 courses of carboplatin therapy. Six of these 19 patients developed carboplatin-related HR during the study period. The CD203c+ basophils (%) and the mean fluorescence intensity (MFI) were analyzed on a flow cytometer and compared between patients with and without HR. Changes in the CD203c expression on basophils before and after HR were also assessed in patients who developed HR during the study period. The median CD203c+ basophils (%) and ΔMFI after 30-min exposure to 50 µg/mL carboplatin were significantly higher in patients with HR (3.5% and ΔMFI 9.0) compared with those without (2.2% and ΔMFI 0.4) (p<0.05). In particular, these values were significantly higher in patients with grade 4 anaphylaxis (10.6% and ΔMFI 22.0). All five patients who developed grade 2-4 anaphylaxis during the study period had high CD203c+ basophils (%) and/or increased ΔMFI on the day before HR. The results suggest that basophil CD203c may be a promising biomarker for the prediction of severe carboplatin-related anaphylaxis.


Asunto(s)
Antineoplásicos/efectos adversos , Basófilos/metabolismo , Carboplatino/efectos adversos , Neoplasias de los Genitales Femeninos/tratamiento farmacológico , Hipersensibilidad/etiología , Hidrolasas Diéster Fosfóricas/inmunología , Pirofosfatasas/inmunología , Adulto , Anciano , Anciano de 80 o más Años , Anafilaxia/inducido químicamente , Anafilaxia/inmunología , Anafilaxia/metabolismo , Antineoplásicos/inmunología , Antineoplásicos/uso terapéutico , Biomarcadores/metabolismo , Carboplatino/inmunología , Estudios de Casos y Controles , Femenino , Humanos , Hipersensibilidad/inmunología , Hipersensibilidad/metabolismo , Persona de Mediana Edad , Hidrolasas Diéster Fosfóricas/metabolismo , Estudios Prospectivos , Pirofosfatasas/metabolismo
4.
Sci Rep ; 11(1): 13711, 2021 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-34211007

RESUMEN

With a sound sensing system using stochastic resonance (4SR), it became possible to obtain an acoustic pulse wave (APW)-a waveform created via a mixture of apex beat and heart sound. We examined 50 subjects who were healthy, with no underlying cardiovascular diseases. We could determine boundary frequency (BF) using APW and phonocardiogram signals. APW data was divided into two bands, one from 0.5 Hz to BF, and a second one from BF to 50 Hz. This permitted the extraction of cardiac apex beat (CAB) and cardiac acoustic sound (CAS), respectively. BF could be expressed by a quadratic function of heart rate, and made it possible to collect CAB and CAS in real time. According to heart rate variability analysis, the fluctuation was 1/f, which indicated an efficient cardiac movement when heart rate was 70 to 80/min. In the frequency band between 0.5 Hz and BF, CAB readings collected from the precordial region resembled apex cardiogram data. The waveforms were classified into five types. Therefore, the new 4SR sensing system can be used as a physical diagnostic tool to obtain biological pulse wave data non-invasively and repeatedly over a long period, and it shows promise for broader applications, including AI analysis.


Asunto(s)
Frecuencia Cardíaca , Cinetocardiografía , Adulto , Femenino , Ruidos Cardíacos , Humanos , Masculino , Persona de Mediana Edad , Sonido , Procesos Estocásticos , Adulto Joven
5.
Intern Med ; 60(20): 3239-3243, 2021 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-33896867

RESUMEN

Accurate genotyping is important to improve the treatment of hepatitis C virus (HCV) infection. We herein report a 44-year-old Japanese man with hemophilia A and coinfection of HCV and human immunodeficiency virus (HIV) who was diagnosed with HCV genotype 4 by direct sequencing. Two genotyping tests based on the nested polymerase chain reaction method that we used misdiagnosed his genotype as 2b and 1b. Although several HCV genotyping tests are available in Japan, it is important to recognize that some cannot detect genotype 4. Care should be taken when genotyping HCV patients who have received non-heated coagulation factor preparations or were infected abroad.


Asunto(s)
Coinfección , Infecciones por VIH , Hepatitis C , Adulto , Coinfección/diagnóstico , Genotipo , VIH/genética , Infecciones por VIH/complicaciones , Infecciones por VIH/diagnóstico , Hepacivirus/genética , Hepatitis C/complicaciones , Hepatitis C/diagnóstico , Humanos , Masculino , Reacción en Cadena de la Polimerasa
6.
Mov Disord ; 24(16): 2408-11, 2009 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-19890970

RESUMEN

Few cases of dopamine agonist-induced antecollis in Parkinson's disease (PD) have been reported. Literature review of 16 PD patients including our 3 cases with dopamine agonist-induced antecollis showed predominance of (1) Japanese, (2) women, and (3) Hoehn-Yahr stage of >or=3. We experienced three Japanese PD patients who subacutely exhibited antecollis following increased dopamine agonist dose that improved just after withdrawal of the agonist. One patient developed antecollis during increasing pramipexole dose in combination with cabergoline. Antecollis in another patient appeared during increasing pramipexole dose; it worsened after substituting pergolide for pramipexole, but improved after withdrawal of pergolide. Our cases indicate that there is no specific dopamine agonist causing antecollis, and it is possibly caused by a number of single dopamine agonists or a combination of them. Dopamine agonist-induced antecollis should be considered when encountering antecollis in PD patients being treated with dopamine agonists and withdrawal of the agonist can improve symptoms.


Asunto(s)
Agonistas de Dopamina/efectos adversos , Discinesia Inducida por Medicamentos/etiología , Músculos del Cuello/fisiopatología , Adulto , Anciano , Agonistas de Dopamina/uso terapéutico , Discinesia Inducida por Medicamentos/patología , Femenino , Humanos , Persona de Mediana Edad , Enfermedad de Parkinson/tratamiento farmacológico
7.
Int Immunopharmacol ; 33: 55-62, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26854577

RESUMEN

Basophil activation test (BAT) is an in vitro allergy test that is useful to identify allergens that cause IgE-dependent allergies. The test has been used to detect not only food allergies and allergies caused by environmental factors but also to detect drug hypersensitivity, which has been known to include IgE-independent reactions. In our preliminary studies in which BAT was applied to detect hypersensitivity of loxoprofen, a non-steroidal anti-inflammatory drug (NSAID), conventional BAT with incubation for 30min did not show basophil activation by means of increased CD203c expression. In this study, we extended the incubation time to 24h on the basis of the hypothesis that loxoprofen indirectly activates basophils. Basophils from healthy control donors as well as allergic patients showed up-regulation of CD203c after incubation with loxoprofen for 24h. Activation was induced using loxoprofen-treated serum. Proteomic and pharmacologic analyses revealed that serum incubation with loxoprofen generated an active complement component C5a, which induced CD203c expression via binding to the C5a receptor on basophils. Because C3a production was also detected after incubation for 24h, loxoprofen is likely to stimulate the complement classical pathway. Our findings suggest that the complement activation is involved in drug hypersensitivity and the suppression of this activation may contribute to the elimination of false positive of BAT for drug allergies.


Asunto(s)
Alérgenos/inmunología , Antiinflamatorios no Esteroideos/inmunología , Basófilos/efectos de los fármacos , Activación de Complemento/efectos de los fármacos , Complemento C5/biosíntesis , Hipersensibilidad a las Drogas/diagnóstico , Fenilpropionatos/inmunología , Antiinflamatorios no Esteroideos/uso terapéutico , Prueba de Desgranulación de los Basófilos , Basófilos/fisiología , Células Cultivadas , Complemento C3a/biosíntesis , Reacciones Falso Positivas , Humanos , Inmunoglobulina E/sangre , Fenilpropionatos/uso terapéutico , Hidrolasas Diéster Fosfóricas/metabolismo , Pirofosfatasas/metabolismo
8.
Transplantation ; 79(7): 821-7, 2005 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-15818325

RESUMEN

BACKGROUND: The prognosis of advanced pancreatic cancer is extremely poor and therefore a novel treatment strategy is desired. The authors thus started a prospective study of allogeneic reduced-intensity hematopoietic stem cell transplantation (RIST) for patients with advanced pancreatic cancer to evaluate the feasibility and efficacy of this approach for such patients. METHODS: Only patients with pathologically proven pancreatic cancer that was locally advanced or metastatic and not amenable to curative resection were included. The conditioning regimen consisted of gemcitabine, fludarabine, and busulfan. RESULTS: In the first stage of this study, the authors treated seven patients. Treatment-related mortality before day 100 was observed in one patient. The median survival after RIST was 229 days. An objective response on computed tomographic scan was observed in two patients and another had a tumor marker response. Marked tumor shrinkage was observed in one of the remaining patients after donor lymphocyte infusion. These antitumor effects appeared after the effect of the conditioning regimen had disappeared. In addition, some of these responses were associated with an increase in the serum anticarcinoembryonic antigen antibody level. CONCLUSIONS: Pancreatic cancer appeared to be sensitive to a graft-versus-tumor effect; therefore, a larger clinical study with a refined strategy is warranted.


Asunto(s)
Efecto Injerto vs Tumor/inmunología , Neoplasias Pancreáticas/inmunología , Neoplasias Pancreáticas/terapia , Trasplante de Células Madre , Adulto , Anciano , Anticuerpos/inmunología , Antígeno Carcinoembrionario/inmunología , Antígeno Carcinoembrionario/metabolismo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Neoplasias Pancreáticas/cirugía , Tasa de Supervivencia , Tomógrafos Computarizados por Rayos X , Acondicionamiento Pretrasplante , Trasplante Homólogo , Resultado del Tratamiento
9.
Intern Med ; 44(2): 129-35, 2005 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-15750273

RESUMEN

A 69-year-old man with a recurrent ventricular tachycardia (VT) was admitted. The patient was diagnosed as myotonic dystrophy type 1 (DM1) and DNA analysis revealed 1,800 CTG-repeat expansion in the myotonic dystrophy protein kinase (DMPK) gene. Ultrasonic cardiogram (UCG), left ventriculogram (LVG) and magnetic resonance imaging (MRI) did not show any abnormal sign including fatty infiltration. But, endomyocardial biopsy obtained from ventricular outflow tract revealed severe fatty infiltration and interstitial fibrosis. Radiofrequency catheter ablation at the biopsy site could eliminate VT, so it was strongly suggested that the re-entry circuit was formed by focal fatty-fibrosis. Careful observation should be continued for a long period.


Asunto(s)
Tejido Adiposo/patología , Cardiomiopatías/complicaciones , Frecuencia Cardíaca/fisiología , Distrofia Miotónica/complicaciones , Taquicardia Ventricular/etiología , Anciano , Biopsia , Cardiomiopatías/diagnóstico , Ablación por Catéter , ADN/análisis , Diagnóstico Diferencial , Electrocardiografía , Fibrosis , Ventrículos Cardíacos/diagnóstico por imagen , Ventrículos Cardíacos/patología , Humanos , Imagen por Resonancia Magnética , Masculino , Distrofia Miotónica/diagnóstico , Distrofia Miotónica/enzimología , Proteína Quinasa de Distrofia Miotónica , Proteínas Serina-Treonina Quinasas/genética , Ventriculografía con Radionúclidos , Taquicardia Ventricular/fisiopatología , Taquicardia Ventricular/cirugía , Ultrasonografía
10.
Anticancer Res ; 35(2): 881-9, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25667470

RESUMEN

BACKGROUND/AIM: Accumulating evidence shows that various types of cancers induce a specific immune response resulting in the production of antibodies against self-components (autoantibodies). The aim of the present study was to identify antigens for autoantibodies in sera from patients with ovarian cancer, especially clear cell carcinoma (CCC), as novel diagnostic markers for the disease. MATERIALS AND METHODS: The reactivity of individual sera from patients was examined by two-dimensional (2-D) immunoblotting using lysates of CCC cell lines, ES-2 and RMG-1, as antigens to identify autoantigens. ELISA was established to quantitatively measure autoantibody titer of patients' sera. RESULTS: Autoantibodies against RhoGDI were induced in sera of ovarian cancer patients. Elevated levels of autoantibodies against heterogeneous nuclear ribonucleoprotein L (hnRNPL) and a mitochondrial protein, dihydrolipoamide dehydrogenase (DLD), were detected in patients with CCC. CONCLUSION: Autoantibodies against RhoGDI and hnRNPL and DLD may serve as novel diagnostic markers for ovarian cancer and CCC, respectively.


Asunto(s)
Autoanticuerpos/sangre , Biomarcadores de Tumor/sangre , Neoplasias Ováricas/inmunología , Proteómica , Autoanticuerpos/genética , Clonación Molecular , Electroforesis en Gel Bidimensional , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Persona de Mediana Edad , Neoplasias Ováricas/sangre
11.
J Virol Methods ; 100(1-2): 107-14, 2002 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-11742657

RESUMEN

Norwalk-like viruses (NLV) are a major causative agent of nonbacterial gastroenteritis. There are still many NLV strains that are refractory to gene amplification by ordinary reverse transcription-polymerase chain reaction. This is due mainly to the genetic diversity among NLV, especially mismatches in the primer sequences, which limits this technique in clinical utility. In this study, improved primer sets based on the capsid region, to detect both genogroup I and II NLV by genogroup-specific manner, were developed. When stool specimens from gastroenteritis patients, that were positive for NLV by electron microscopy, were tested by this new primer set, all specimens were positive by RT-PCR. Primers described previously for RdRp and capsid protein were capable of amplifying the specimens by 31 and 77%, respectively. Therefore, new primer sets are extremely useful for the amplification and rapid diagnosis of nonbacterial gastroenteritis due to NLV as well as for epidemiological studies.


Asunto(s)
Infecciones por Caliciviridae/virología , Proteínas de la Cápside , Cápside/genética , Cartilla de ADN , Brotes de Enfermedades , Gastroenteritis/virología , Norovirus/aislamiento & purificación , ARN Viral/análisis , ARN Polimerasa Dependiente del ARN/genética , Secuencia de Bases , Infecciones por Caliciviridae/epidemiología , ADN Viral , Gastroenteritis/epidemiología , Humanos , Japón/epidemiología , Datos de Secuencia Molecular , Norovirus/clasificación , Norovirus/genética , Reacción en Cadena de la Polimerasa/métodos , Sensibilidad y Especificidad
12.
Rinsho Shinkeigaku ; 43(6): 313-21, 2003 Jun.
Artículo en Japonés | MEDLINE | ID: mdl-14503348

RESUMEN

Angiographic, CT and MR findings, precipitating factors, and clinical features of spontaneous intracranial internal carotid artery dissection, in particular the terminal segment of the ICA, are described. Patients who had a possible posttraumatic dissection were excluded from the study. Six patients, 3 men and 3 women, mean age 25.8 years, were studied. Spontaneous ICA dissection accounted for 0.63 percent of 797 patients with ischemic stroke and 14 percent of 36 patients with ischemic stroke who were 45 years of age or younger. Headache was absent in two but clearly preceded the onset of cerebral stroke in four patients, the interval ranging from less than a few minutes to 6 hours. Focal cerebral ischemic symptoms were present in all six patients. Four presented with loss of consciousness. Only one patient had a subarachnoid hemorrhage (SAH). Dissection occurred at the level of the carotid knee portion just beyond the origin of the ophthalmic artery in all the patients. Moreover, there was additional involvement of the ipsilateral MCA in four and ipsilateral ACA in three. Angiographic features of the six ICAs that involved dissection were a smooth tapered luminal narrowing or occlusion in two patients, irregular narrowing in three, a pearl and string sign in one, and retention of the contrast media in three. Follow up angiography or magnetic resonance (MR) angiography was performed on all the patients. The interval from the initial to follow-up angiography was one to six months. Stenosis persisted in one artery but was resolved in the other five arteries. MR angiography showed segmental narrowing of the right supraclinoid internal carotid artery in one patient. MR angiographic source images showed a narrow lumen in the right intracavernous portion of the ICA and a crescent-shaped filling defect. Follow-up MR imaging and MR angiography, 6 months after the initial study, showed amelioration of the luminal narrowing and no filling defect. The filling defect is considered to be due to a thrombosed pseudolumen. Treatment consisted of anticoagulation therapy for two patients, hyperbaric therapy for two, and supportive measures only for two. Follow-up ranged from ten months to three years. Of these six patients with completed stroke, four had full recovery, and two some residual neurological deficits. Trivial precipitating events were reported by five of the patients (playing tennis, running, bicycling, and drinking alcohol). Migraine headaches were cited by one patient, use of oral contraceptives by another, and use of tobacco by a third. All the patients were normotensive. In none of them was family history contributory, nor was any underlying arteriopathy identified. Of the six personal cases and the reviewed 25 cases during the last 15 years, represented on the Glasgow Outcome Scale, good recovery was achieved in 61%. The outcomes for these patients was better than previously reported. Spontaneous intracranial ICA dissection may cause ischemic stroke, with or without subarachnoid hemorrhage, especially in young patients.


Asunto(s)
Disección de la Arteria Carótida Interna/diagnóstico , Adolescente , Adulto , Factores de Edad , Disección de la Arteria Carótida Interna/complicaciones , Disección de la Arteria Carótida Interna/epidemiología , Disección de la Arteria Carótida Interna/terapia , Angiografía Cerebral , Infarto Cerebral/etiología , Femenino , Cefalea/etiología , Humanos , Angiografía por Resonancia Magnética , Masculino , Pronóstico , Factores de Riesgo , Factores Sexuales , Tomografía Computarizada por Rayos X
13.
Rinsho Shinkeigaku ; 42(2): 131-5, 2002 Feb.
Artículo en Japonés | MEDLINE | ID: mdl-12424962

RESUMEN

A case of parkinsonian syndrome caused by normal pressure hydrocephalus (NPH) accompanied by cauda equina neurinoma is reported. A 69-year-old woman presented with typical symptoms of parkinsonism, including akinesia, resting and postual tremor, and cog-wheel rigidity. CT scan of the brain revealed dilatation of ventricles, but she did not present dementia and urinary incontinence that are common symptoms in NPH. Her cerebrospinal fluid (CSF) pressure was normal, and her protein level was high at 2,970 mg/dl. An electroencephalogram (EEG) showed diffuse slow waves. An IMP-SPECT images of the brain showed diffuse reduction of radioisotope uptake. Levodopa was not effective in treating her parkinsonism. Removal of the tumor caused dramatic improvement in her parkinsonism. Her CSF protein level was normalized and EEG and SPECT images were improved after the operation. However, ventricular size on brain CT showed no change. It was considered that the causal mechanism of NPH was due to high protein levels in the CSF. The parkinsonism in this case was caused by dysfunction of the circuits linking the cortex, basal ganglia, and thalamus associated with metabolic disorder due to periventricular ischemia. Typical parkinsonism caused by NPH associated with spinal cord tumor has not been reported. When we examine a patient with parkinsonian syndrome caused by NPH, we should check the CSF protein level. And if that level is high, the possibility of spinal cord tumor should be considered.


Asunto(s)
Cauda Equina , Hidrocéfalo Normotenso/complicaciones , Neurilemoma/complicaciones , Enfermedad de Parkinson Secundaria/etiología , Neoplasias del Sistema Nervioso Periférico/complicaciones , Femenino , Humanos , Persona de Mediana Edad
14.
No To Shinkei ; 55(6): 517-20, 2003 Jun.
Artículo en Japonés | MEDLINE | ID: mdl-12884804

RESUMEN

A-65-year old woman who developed total ophthalmoplegia, areflexia in all her limbs and ataxia after upper respiratory tract infection was admitted to our hospital on the second day of illness. On day 3, she developed severe tetraparesis and respiratory failure which required mechanical ventilation, and Guillain-Barré syndrome (GBS) was diagnosed. On day 4, bilateral ptosis, facial diplegia, and neck muscle weakness appeared, and her all limbs were flaccid and immobile. An electrophysiological study suggested axonal damage. Marked blood pressure fluctuation also appeared on day 4. On day 5, an electrocardiogram showed a ST-segment elevation in leads V1 through V4 and the echocardiography showed anterior hypokinesia of the left ventricle. Her serum creatine kinase was normal. Left ventricular dysfunction and ST-segment elevation were normalized within hours, but a transient ST-segment elevation re-occurred on day 6. An electrocardiogram on day 13, showed inverted T waves in diffuse leads, which inversion continued. ST-segment elevation and hypokinesia in this patient were restricted to the left anterior descending branch, therefore, coronary spasm of that branch was considered the possible mechanism. In contrast, inverted T wave was due to either catecholamine cardiotoxicity or diffuse cardiac ischemia. Abnormalities of electrocardiogram were presumably due to cardiovascular autonomic dysfunction of GBS.


Asunto(s)
Electrocardiografía , Síndrome de Guillain-Barré/fisiopatología , Hipocinesia/fisiopatología , Disfunción Ventricular Izquierda/fisiopatología , Anciano , Presión Sanguínea , Vasoespasmo Coronario/fisiopatología , Femenino , Humanos , Contracción Miocárdica , Función Ventricular Izquierda
15.
Anticancer Res ; 34(9): 5021-7, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25202086

RESUMEN

BACKGROUND/AIM: Accumulating evidence shows that various types of cancers induce a specific immune response, resulting in the production of antibodies against self-components (autoantibodies). The aim of the present study was to identify antigens for autoantibodies in sera from endometrial cancer patients as novel diagnostic markers for the disease. MATERIALS AND METHODS: The reactivity of individual sera from patients was examined by 2-dimensional (2-D) immunoblotting using HeLa cell lysates as antigens to identify autoantigens. ELISA was established to quantitatively measure autoantibody titer of patients' sera. RESULTS: A mitochondrial protein, dihydrolipoamide dehydrogenase (DLD), was identified as an autoantigen specific to endometrial cancer patients. The levels of immunoglobulin (Ig)A but not IgG autoantibody to DLD were significantly increased in the sera of endometrial cancer patients. CONCLUSION: IgA autoantibody against DLD could be a novel diagnostic marker for endometrial cancer.


Asunto(s)
Autoanticuerpos/inmunología , Dihidrolipoamida Deshidrogenasa/inmunología , Neoplasias Endometriales/inmunología , Proteómica , Adulto , Anciano , Biomarcadores de Tumor/inmunología , Estudios de Casos y Controles , Línea Celular , Dihidrolipoamida Deshidrogenasa/metabolismo , Neoplasias Endometriales/diagnóstico , Neoplasias Endometriales/metabolismo , Femenino , Humanos , Inmunoglobulina A/inmunología , Persona de Mediana Edad , Estadificación de Neoplasias , Proteómica/métodos , Neoplasias del Cuello Uterino/inmunología , Neoplasias del Cuello Uterino/metabolismo
16.
Intern Med ; 52(20): 2347-9, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24126398

RESUMEN

Pseudoperipheral palsy can be caused by cerebral cortical infarctions; however, it is rarely caused by lacunar infarctions, including those in the posterior limb of the internal capsule. Meanwhile, the somatotopic localization of the corticospinal tract in the posterior limb of the internal capsule remains unknown. We herein report the case of an 81-year-old Japanese woman who presented with a left hand drop. Brain magnetic resonance imaging revealed an acute infarction as the causative lesion at the inferior level of the anteromedial portion of the posterior limb of the right internal capsule. This case report indicates the topography of hand fibers in the internal capsule.


Asunto(s)
Infarto Cerebral/diagnóstico , Mano/patología , Cápsula Interna/patología , Parálisis/diagnóstico , Anciano de 80 o más Años , Infarto Cerebral/complicaciones , Femenino , Humanos , Parálisis/etiología
17.
IEEE Trans Inf Technol Biomed ; 15(1): 19-25, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21075732

RESUMEN

Systems capable of monitoring the biological condition of a driver and issuing warnings during instances of drowsiness have recently been studied. Moreover, many researchers have reported that biological signals, such as brain waves, pulsation waves, and heart rate, are different between people who have and have not consumed alcohol. Currently, we are developing a noninvasive system to detect individuals driving under the influence of alcohol by measuring biological signals. We used the frequency time series analysis to attempt to distinguish between normal and intoxicated states of a person as the basis of the sensing system.


Asunto(s)
Intoxicación Alcohólica/diagnóstico , Conducción de Automóvil , Conducta Peligrosa , Monitoreo Fisiológico/métodos , Pletismografía/métodos , Procesamiento de Señales Asistido por Computador , Intoxicación Alcohólica/fisiopatología , Algoritmos , Pruebas Respiratorias , Femenino , Humanos , Masculino , Monitoreo Fisiológico/instrumentación , Pletismografía/instrumentación , Factores de Tiempo , Adulto Joven
19.
J Neurol Sci ; 281(1-2): 108-9, 2009 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-19339022

RESUMEN

We report the case of a 43-year-old woman who developed multiple cranial nerve palsy, the symptoms of which included hyposmia, visual loss, facial hypoesthesia, facial weakness, dysphagia, gustatory disturbance, and sensory disturbance of the trunk and ulnar side of the bilateral arms. The clinical features included swelling of the bilateral hilar lymph nodes, uveitis, an elevated serum angiotensin-converting enzyme level, and negative tuberculin reactions, which led to a diagnosis of neurosarcoidosis. Her symptoms improved after administration of steroids. An elevated cerebrospinal fluid cell count and protein level, a low-frequency F-wave and slightly decreased sensory nerve action potentials in bilateral ulnar nerves by nerve conduction studies, and normal findings in the spine by magnetic resonance imaging suggested that truncal hypoesthesia was caused by polyradiculopathy. Although rare, in patients with neurosarcoidosis, truncal polyradiculopathy is noteworthy findings in addition to cranial nerve palsy.


Asunto(s)
Enfermedades de los Nervios Craneales/etiología , Polirradiculopatía/etiología , Sarcoidosis/complicaciones , Adulto , Enfermedades de los Nervios Craneales/fisiopatología , Diagnóstico Diferencial , Femenino , Humanos , Conducción Nerviosa , Polirradiculopatía/fisiopatología , Sarcoidosis/diagnóstico , Sarcoidosis/fisiopatología
20.
J Cardiol ; 54(2): 307-10, 2009 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-19782271

RESUMEN

This is a case of a thrombo-occlusion occurring below an inferior vena cava (IVC) filter inserted in a patient with polycythemia vera and deep vein thrombosis. The patient was a 48-year-old man with polycythemia vera and a chief complaint of swelling, redness, and bursting pain in his right leg. After admission, contrast-enhanced computed tomography scanning demonstrated a pulmonary artery thrombus and deep vein thrombosis. We inserted a Gunther tulip vena cava filter on day 1 for the prevention of pulmonary embolism (PE), and started anticoagulation therapy based on the guideline of the Japanese Circulation Society for DVT. In addition to intravenous anticoagulants, we started therapeutic phlebotomy to improve the hypercoagulability state. On day 4, our patient complained of back pain caused by thrombo-occlusion below the IVC filter, despite the anticoagulation therapy and two therapeutic phlebotomies. From this case, we concluded it is important to lower hemoglobin level and hematocrit as early as possible for IVC-filter-insertion in patients with polycythemia vera.


Asunto(s)
Policitemia Vera/complicaciones , Policitemia Vera/terapia , Embolia Pulmonar/etiología , Filtros de Vena Cava/efectos adversos , Vena Cava Inferior , Trombosis de la Vena/etiología , Anticoagulantes/administración & dosificación , Hematócrito , Hemoglobinas , Humanos , Masculino , Persona de Mediana Edad , Flebotomía , Policitemia Vera/sangre , Embolia Pulmonar/prevención & control , Resultado del Tratamiento , Trombosis de la Vena/prevención & control
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA