Tree architecture: A strigolactone-deficient mutant reveals a connection between branching order and auxin gradient along the tree stem.

Due to their long lifespan, trees and bushes develop higher order of branches in a perennial manner. In contrast to a tall tree, with a clearly defined main stem and branching order, a bush is shorter and has a less apparent main stem and branching pattern. To address the developmental basis of these two forms, we studied several naturally occurring architectural variants in silver birch (Betula pendula). Using a candidate gene approach, we identified a bushy kanttarelli variant with a loss-of-function mutation in the BpMAX1 gene required for strigolactone (SL) biosynthesis. While kanttarelli is shorter than the wild type (WT), it has the same number of primary branches, whereas the number of secondary branches is increased, contributing to its bush-like phenotype. To confirm that the identified mutation was responsible for the phenotype, we phenocopied kanttarelli in transgenic BpMAX1::RNAi birch lines. SL profiling confirmed that both kanttarelli and the transgenic lines produced very limited amounts of SL. Interestingly, the auxin (IAA) distribution along the main stem differed between WT and BpMAX1::RNAi. In the WT, the auxin concentration formed a gradient, being higher in the uppermost internodes and decreasing toward the basal part of the stem, whereas in the transgenic line, this gradient was not observed. Through modeling, we showed that the different IAA distribution patterns may result from the difference in the number of higher-order branches and plant height. Future studies will determine whether the IAA gradient itself regulates aspects of plant architecture.

Formal description of plant morphogenesis.

Plant morphogenesis may be characterized by complex feedback mechanisms between signals specifying growth and by the growth of the plant body itself. Comprehension of such feedback mechanisms is an ongoing research task and can be aided with formal descriptions of morphogenesis. In this review, we present a number of established mathematical paradigms that are useful to the formal representation of plant shape, and of biomechanical and biochemical signaling. Specifically, we discuss work from a range of research areas including plant biology, material sciences, fluid dynamics, and computer graphics. Treating plants as organized systems of information processing allows us to compare these different mathematical methods in terms of their expressive power of biological hypotheses. This is an attempt to bring together a large number of computational modeling concepts and make them accessible to the analytical as well as empirical student of plant morphogenesis.