Delta of Exopalaemon carinicauda: molecular characterization, expression in different tissues and developmental stages, and its SNPs association analysis with development.

BACKGROUND: The Notch signaling pathway plays a significant role in the gene regulatory network of development of vertebrate and invertebrate. However, as a ligand for the Notch signaling pathway, the mechanism of Delta in the development of Exopalaemon carinicauda is still unclear. METHODS AND RESULTS: The Delta's molecular characteristics, tissue distribution and their association with development in E. carinicauda were studied by RACE (rapid amplification of cDNA end), qRT-PCR (quantitative Real-time PCR) and SNP (single nucleotide polymorphism), respectively. The delta in E. carinicauda had a full-length cDNA of 2807 bp and its Delta of 808 amino-acid residue had the highest identity with the Delta of Homarus americanus (identity = 76.63%). Delta had the highest expression in the ovary, and its expression varied with different stages of embryonic, larval, and ovarian development. After delta RNA interference (with a highest interference efficiency of 66% at 24 h), the expression of Notch signaling pathway genes and developmental related genes was significantly reduced, and the ovarian development was significantly delayed. Further study found that there were 4 SNPs (ds1-4) in delta cDNA, of which two (ds2 T1521G caused a mutation Asn422Lys and ds3 G1674A caused a mutation Tyr473Cys in the EGF-like domain) were associated with the development of E. carinicauda. The Gonadosomatic Index (GSI) of the ds2 TT genotypes was 37.28% and 134.60% higher than E. carinicauda of GT and GG genotype respectively (P < 0.05). CONCLUSION: Our research indicated that delta was involved in the development of E. carinicauda and provided new insights for molecular breeding with SNP markers in E. carinicauda.

A novel Bacillus sp. with antagonistic activity against a plant pathogen, Fusarium graminearum, and its potential antagonistic mechanism.

Fusarium head blight (FHB) is a wheat disease caused by the plant pathogen Fusarium graminearum, which leads to crop yield losses and agricultural economic losses, as well as poses a threat to the environment and human health. Effective biocontrol of F. graminearum is urgent. An antagonistic strain HZ-5 with 59.2% antagonistic activity against F. graminearum in vitro had been isolated from sea mud of Haizhou Bay using a dual-culture assay, which was highly homologous with Bacillus halosaccharovorans according to the 16S rRNA sequence. The antagonistic activity of HZ-5 had been further studied. HZ-5 had a broad range of antagonistic activity against another six plant pathogenic fungi and was effective in controlling FHB of wheat in pot experiment. The substances with antagonistic activity were temperature insensitive, and had been purified by HPLC (High Performance Liquid Chromatography) to prove to be secreted lipopeptides. The antagonistic substances induced the biosynthesis of chitin and glycerol, while ergosterol , cholesterol, and phosphatidylcholine reduced their inhibitory effects on F. graminearum. These data would be helpful to provide a better biocontrol strain against FHB, and to provide important basis to elucidate the antagonistic mechanism of biocontrol.

Oxidative Stress and Endoplasmic Reticulum Stress Contributes to Arecoline and Its Secondary Metabolites-Induced Dyskinesia in Zebrafish Embryos.

Areca nut has been listed as one of the most addictive substances, along with tobacco, alcohol and caffeine. Areca nut contains seven psychoactive alkaloids; however, the effects of these alkaloids on embryonic development and motor behavior are rarely addressed in zebrafish embryo-larvae. Herein, we investigated the effects of exposure to three alkaloids (arecoline and secondary metabolites-arecaidine and arecoline N-oxide) on the developmental parameters, locomotive behavior, oxidative stress and transcriptome of zebrafish embryos. Zebrafish embryos exposed to different concentrations (0, 0.1, 1, 10, 100 and 1000 µM) of arecoline, arecaidine and arecoline N-oxide showed no changes in mortality and hatchability rates, but the malformation rate of zebrafish larvae was significantly increased in a dose-dependent manner and accompanied by changes in body length. Moreover, the swimming activity of zebrafish larvae decreased, which may be due to the increase in reactive oxygen species and the imbalance between oxidation and antioxidation. Meanwhile, transcriptome analysis showed that endoplasmic reticulum stress and the apoptosis p53 signaling pathway were significantly enriched after exposure to arecoline and arecoline N-oxide. However, arecaidine exposure focuses on protein synthesis and transport. These findings provide an important reference for risk assessment and early warning of areca nut alkaloid exposure.

Expression and function analysis of crustacyanin gene family involved in resistance to heavy metal stress and body color formation in Exopalaemon carinicauda.

Crustacyanin has the function of binding astaxanthin which is the best antioxidant, and plays an important role in the body color variation of crustaceans. To investigate the causes of body color variation of the ridgetail white prawn, Exopalaemon carinicauda, the present study obtained four subtypes of crustacyanin gene: C1, C2, A1, and A2. Based on fluorescence quantitative polymerase chain reaction, lipocalin-C1 is mainly expressed in the eyestalk, lipocalin-C2 is in the ventral nerve cord, and lipocalin-A1 and lipocalin-A2 are in subcutaneous adipose tissues. Under the inhibiting effect of Cd2+ stress, the expression of four subtypes first increases and then decreases within 24 h, and reaches the maximum at 6 or 12 h. RNA interference experiments showed a decrease in the expression of lipocalin genes in subcutaneous adipose tissue for each subtype, with the body color changing from transparent to red, and the dark red spots on the epidermis changing to bright red. Moreover, the blue protein in the subcutaneous adipose tissue largely disappeared, based on the light micrographs. In view of these findings, the crustacyanin gene appears to fulfill some function in the resistance to heavy metal stress and body color formation of E. carinicauda.

NaOH-Intercalated Iron Chalcogenides (Na1-xOH)Fe1-yX (X = Se, S): Ion-Exchange Synthesis and Physical Properties.

The discovery of the (Li1-xFexOH)FeSe superconductor has aroused significant interest in metal hydroxide-intercalated iron chalcogenides. However, all efforts made to intercalate NaOH between FeSe and FeS layers have failed so far. Here we report two NaOH-intercalated iron chalcogenides (Na1-xOH)Fe1-yX (X = Se, S) that were synthesized by a low-temperature hydrothermal ion-exchange method. Their crystal structures were solved through single-crystal X-ray diffraction and refined against powder X-ray and neutron diffraction data. Different from the (Li1-xFexOH)FeX superconductors that crystallize in a tetragonal space group P4/nmm with Z = 2, (Na1-xOH)Fe1-yX belong to an orthorhombic space group Cmma with Z = 4. The structural solution also reveals that there are vacancies in both Na and Fe sites and there are not iron ions in the (Na1-xOH) layer. This is probably why both Fe(II) and Fe(III) species exist in the title compounds, as detected by X-ray photoelectron spectroscopy. Based on magnetization and electrical resistivity measurements, the two compounds were found to be paramagnetic semiconductors. The absence of superconductivity should be closely related to the iron vacancies in the Fe1-yX layer. Theoretical calculations suggest that inducing superconductivity in (Na1-xOH)Fe1-ySe is promising due to the similarity of the electronic structures between stoichiometric (NaOH)FeSe and the (Li1-xFexOH)FeSe superconductor.

Cloning and functional study of lipocalin: retinol-binding protein-like gene family of the ridgetail white prawn, Exopalaemon carinicauda.

Lipocalin is a large family with complex functions including retinol-binding protein (RBP), crustacyanin (CRCN), apolipoprotein D, etc. In shrimps, it is well known that CRCN is related to body color. Recently, retinoic acid/retinol-binding protein was found in shrimp. However, little is known about the function of RBP and relationships among the gene members of lipocalin in shrimps. Based on the transcriptome sequences responding to starvation stress, three genes of the lipocalin-retinol-binding protein-like gene family (lipocalin-1, lipocalin-2, and lipocalin-3) were cloned by RACE from the ridgetail white prawn, Exopalaemon carinicauda. Homology analysis showed that these three genes had high similarity with the known insect apolipoprotein D gene and vertebrate retinol-binding protein gene, and they are of the same type in terms of evolution. Fluorescence quantitative PCR showed that the above three genes were mainly expressed in the ventral nerve cord of E. carinicauda. The expression characteristics of the three genes at different developmental stages showed that they were more highly expressed at the larval stage, which suggests that they might be related to embryonic and larval development. The RNA interference tests showed that after silencing lipocalin-1 and lipocalin-3, the body color of individual shrimps turned slightly red and the blue pigment in the epidermis largely disappeared, but no significant change took place in the appearance of individuals after silencing lipocalin-2. In addition, on the 6th and 16th days of interference, dead shrimps appeared in the lipocalin-1 and lipocalin-3 interference groups. The dead shrimps had hard crusts and remained in a molting posture. Totally, this study showed that the retinol-binding protein-like gene obtained in this study had certain biological functions in the growth and development and body color formation as CRCN; in addition, it also plays a role in nerve system and molting of E. carinicauda.

Enhanced Superconductivity in Restacked TaS2 Nanosheets.

Since interface superconductivity was discovered at the interface between two insulating layers LaAlO3 and SrTiO3, such interface-induced superconducting systems have been a research hotspot in superconductivity. Here, we report homogeneous interfaces formed by stacking chemically exfoliated monolayer TaS2 nanosheets randomly. Enhanced superconductivity of Tc = 3 K is observed, compared with 0.8 K of parent 2H-TaS2. The measurement of heat capacity shows the increase of electronic specific-heat coefficient γ of restacked TaS2 nanosheets compared to parent 2H-TaS2 crystals. Density functional theory calculations indicate that increase and delocalization of electron states near the Fermi surface due to the homogeneous interfaces effects could account for the enhanced superconductivity.

Effects of Iron Doping on the Physical Properties of Quaternary Ferromagnetic Sulfide: Ba2Fe0.6V1.4S6.

The mixed-metal sulfide compound with the formula Ba2Fe0.6V1.4S6 was successfully synthesized via solid-state reaction. Ba2Fe0.6V1.4S6 has a quasi-one-dimensional structure and crystallizes in the hexagonal space group P63/mmc. The structure is composed of face-sharing anion octahedron [MS6]8- (M = V or Fe) units to construct infinite chains along the c axis, in which the Fe atoms randomly occupy the V sites. The Ba2+ ions reside between adjacent chains. Magnetic susceptibility measurements reveal a transition between paramagnetism and ferromagnetism around 25 K. The small polaron hopping (SPH) conduction behavior has been observed in the higher temperature region (75-300 K), while in the lower temperature region (25-74 K), the resistivity features a variable range hopping mechanism (VRH). The analysis of density of states indicates that Fe-3dz2 and S-3p states mainly dominate the valence band maximum, while Fe-3dz2 states contribute significantly to the magnetic susceptibility.

Modulating the Morphology of Gold Graphitic Nanocapsules for Plasmon Resonance-Enhanced Multimodal Imaging.

With their unique optical properties and distinct Raman signatures, graphitic nanomaterials can serve as substrates for surface-enhanced Raman spectroscopy (SERS) or provide signal amplification for bioanalysis and detection. However, a relatively weak Raman signal has limited further biomedical applications. This has been addressed by encapsulating gold nanorods (AuNRs) in a thin graphitic shell to form gold graphitic nanocapsules. This step improves plasmon resonance, which enhances Raman intensity, and has the potential for integrating two-photon luminescence (TPL) imaging capability. However, changing the morphology of gold graphitic nanocapsules such that high quality and stability are achieved remains a challenge. To address this task, we herein report a confinement chemical vapor deposition (CVD) method to prepare the construction of AuNR-encapsulated graphitic nanocapsules with these properties. Specifically, through morphological modulation, we (1) achieved higher plasmon resonance with near-IR incident light, thus achieving greater Raman intensity, and (2) successfully integrated two-photon luminescence dual-modal (Raman/TPL) bioimaging capabilities. Cancer-cell-specific aptamers were further modified on the AuNR@G graphitic surface through simple, but strong, π-π interactions to achieve imaging selectivity through differential cancer cell recognition.

Elucidating the cellular uptake mechanism of aptamer-functionalized graphene-isolated-Au-nanocrystals with dual-modal imaging.

Elucidating the endocytosis and metabolism of nanoparticles in cells could improve the diagnostic sensitivity and therapeutic efficiency. In this work, we explore the cellular uptake mechanism of a biocompatible nanocrystal nanostructure, graphene-isolated-Au-nanocrystals (GIANs), by monitoring the intrinsic Raman and two-photon luminescence signals of GIANs in live cells. Aptamers functionalized on the GIAN nanostructure through simple, but strong, π-π interactions entered the cells through a clathrin-dependent pathway, while unmodified GIANs mainly entered the cells through a caveolae-mediated endocytosis pathway. Thus, it can be concluded that the mechanism of cellular uptake in these graphene-isolated-Au-nanocrystal nanostructures is determined by the presence or absence of aptamer modification.

Observation of Superconductivity in Tetragonal FeS.

The possibility of superconductivity in tetragonal FeS has attracted considerable interest because of its similarities to the FeSe superconductor. However, all efforts made to pursue superconductivity in tetragonal FeS have failed so far, and it remains controversial whether tetragonal FeS is metallic or semiconducting. Here we report the observation of superconductivity at 5 K in tetragonal FeS that is synthesized by the hydrothermal reaction of iron powder with sulfide solution. The obtained samples are highly crystalline and less air-sensitive, in contrast to those reported in the literature, which are meta-stable and air-sensitive. Magnetic and electrical properties measurements show that the samples behave as a paramagnetic metal in the normal state and exhibit superconductivity below 5 K. The high crystallinity and the stoichiometry of the samples play important roles in the observation of superconductivity. The present results demonstrate that tetragonal FeS is a promising new platform to realize high-temperature superconductors.

Genomic organization and promoter analysis of a transcriptional repressor gene from Fenneropenaeus chinensis.

In this study, we cloned and sequenced genomic sequences from a Fenneropenaeus chinensis transcriptional repressor gene, FcTR. The FcTR gene is 2,671 bp in length and has four exons and three introns. The 873 bp promoter contains several transcription factor binding sites, including a TATA box and a cyclic AMP-responsive element. Promoter deletion analysis using a luciferase reporter gene identified regulatory elements. Challenge with white spot syndrome virus increased expression from the promoter-deletion constructs. These results suggest that FcTR might play an important role in the shrimp immune response.

Structures and physical properties of layered oxyselenides Ba(2)MO(2)Ag(2)Se(2) (M = Co, Mn).

Two new layered oxyselenides, Ba2MO2Ag2Se2 (M = Co, Mn), have been successfully synthesized via solid-state reaction. It is found that these two compounds, consisting of the infinite MO2 square planes and antifluorite-type Ag2Se2 layers separated by barium, possess new structural features while keeping I4/mmm symmetry. A detailed calculation on the discrete coordination of transition metals by oxygen in the two compounds and Ba2ZnO2Ag2Se2 revealed quite different energy landscapes. The calculated results indicate that the manganese compound favors adoption of the I4/mmm space group, while the cobalt compound could be at the boundary of the transition between the I4/mmm and Cmca phases. In Ba2CoO2Ag2Se2, the coexistence of a large barium ion and a Ag2Se2 layer expands the oxide layer significantly and results in the largest Co-O bond length in the square-planar sheet ever reported. Ba2CoO2Ag2Se2 is near-stoichiometric, whereas Ba2MnO2Ag2Se2 contains 7% silver vacancies, which is explained by the mixed valence of the manganese ion between 2+ and 3+. In Ba2CoO2Ag2Se2, the zero-field-cooled and field-cooled susceptibilities bifurcate at 159 K, located between two antiferromagnetic (AFM) transitions. Meanwhile, Ba2MnO2Ag2Se2 shows high-temperature Curie-Weiss behavior, followed by a low-temperature AFM transition with TN = 32 K. They both exhibit semiconducting behavior with resisitivities of about 10(5)Ω cm at room temperature. The optical band gaps are determined to be 1.49 and 1.18 eV for Ba2CoO2Ag2Se2 and Ba2MnO2Ag2Se2, respectively. Band structure calculations reveal that Ba2CoO2Ag2Se2 is a direct-gap semiconductor, with a calculated band gap of 1.147 eV; however, Ba2MnO2Ag2Se2 failed to reproduce the semiconducting behavior within an A-type AFM model.

Superconducting phases in potassium-intercalated iron selenides.

The ubiquitous coexistence of majority insulating 245 phases and minority superconducting (SC) phases in A(x)Fe(2-y)Se(2) (A = K, Cs, Rb, Tl/Rb, Tl/K) formed by high-temperature routes makes pure SC phases highly desirable for studying the intrinsic properties of this SC family. Here we report that there are at least two pure SC phases, K(x)Fe(2)Se(2)(NH(3))(y) (x ≈ 0.3 and 0.6), determined mainly by potassium concentration in the K-intercalated iron selenides formed via the liquid ammonia route. K(0.3)Fe(2)Se(2)(NH(3))(0.47) corresponds to the 44 K phase with lattice constant c = 15.56(1) Å and K(0.6)Fe(2)Se(2)(NH(3))(0.37) to the 30 K phase with c = 14.84(1) Å. With higher potassium doping, the 44 K phase can be converted into the 30 K phase. NH(3) has little, if any, effect on superconductivity. Thus, the conclusions should apply to both K(0.3)Fe(2)Se(2) and K(0.6)Fe(2)Se(2) SC phases. K(0.3)Fe(2)Se(2)(NH(3))(0.47) and K(0.6)Fe(2)Se(2)(NH(3))(0.37) stand out among known superconductors as their structures are stable only at particular potassium doping levels, and hence the variation of T(c) with doping is not dome-like.

New layered iron sulfide NaFe(1.6)S2: synthesis and characterization.

Na was intercalated between [Fe2S2] layers for the first time, giving a novel compound NaFe(1.6)S2. This material adopts a CaAl2Si2-type structure with ~20% iron vacancies and represents the first layered compound in a ternary Na-M-X (M = Fe, Co, Ni; X = S, Se) system. First-principles calculations reveal that phonon dynamics is an important factor for it to prefer the CaAl2Si2-type rather than the ThCr2Si2-type structure. It features a magnetic transition at 205 K and is a narrow-band-gap semiconductor.

Identification and molecular characterization of a C-type lectin-like protein from Chinese shrimp (Fenneropenaeus chinensis).

A C-type lectin-like protein was cloned and characterized from the Chinese shrimp Fenneropenaeus chinensis, and named as FcCTL. The results indicated that the full length cDNA of 859 bp had an open reading frame encoded a polypeptide of 220 amino acids with one carbohydrate-recognition domain, six conserved Cys and one key motif EPGD. The theoretical molecular weight and pI of mature protein was 25.3 kDa and 5.4. Sequence comparison of the deduced amino acid sequence of FcCTL showed varied identity of 26-34, 34, 31 and 30 % with those of F. chinensis, Portunus trituberculatus, Tetraodon nigroviridis, Penaeus monodon, respectively. qRT-PCR analysis indicated that FcCTL was expressed highest in hepatopancreas of normal shrimp, and it's expression was up-regulated in hepatopancreas and gills post white spot syndrome virus challenge. The purified recombinant FcCTL showed higher antimicrobial activity against Gram-positive bacteria than against Gram-negative bacteria and fungi. And the hemagglutinating activity of rFcCTL could be completely inhibited by GlcNAc (5 µg/ml), LPS (2.5 µg/ml), D-galactose (100 mM) and maltose (100 mM). These data suggested that FcCTL might play an important role in shrimp immune and would be helpful to better understand the innate immunity mechanism of shrimp.

Effects of the Probiotic Psychrobacter sp. B6 on the Growth, Digestive Enzymes, Antioxidant Capacity, Immunity, and Resistance of Exopalaemon carinicauda to Aeromonas hydrophila.

Probiotics, known to improve the water quality and the host's intestinal microbial balance, has gained more and more attention in recent years. The effects of Psychrobacter sp. B6 on the growth and immunity of Exopalaemon carinicauda were investigated in this study. Psychrobacter sp. B6 was sprayed to the basal diet with four different levels (0 [basal diet], 5 × 105, 5 × 107, and 5 × 109 CFU/100 g diet) and were fed to E. carinicauda (average weight 1.15 ± 0.04 g) for 30 days. At the end of the feeding trial, shrimps were immersed in seawater contaminated with 106 CFU/mL pathogenic Aeromonas hydrophila for 2 h and then the cumulative mortality was calculated after 14 days observation. The results showed that the weight gain rate, survival rate, and specific growth rate of E. carinicauda were significantly increased with the increasing dietary level of Psychrobacter sp. B6. The activities of digestive enzymes (α-amylase and chymotrypsin) were significantly increased (P < 0.05) in the groups fed with Psychrobacter sp. B6, and the highest activities of digestive enzymes were detected in the 5 × 109 CFU/100 g diet group. The activities of antioxidant enzymes (catalase, peroxidase, and superoxide dismutase) in probiotics treated shrimp were significantly higher than those in the control shrimp, with the highest activity in 5 × 109 and 5 × 107 CFU/100 g diet group separately. At the same time, the activities of immune-related enzymes (alkaline phosphatase and lysozyme) were significantly affected by the dietary B6 content, and the highest activity of immune-related enzymes was found in shrimps fed with 5 × 107 CFU/100 g diet. The relative expression levels of CTL (C-type lectin), MBL (mannose-binding lectin), SPI (serine protease inhibitor), and ProPo (prophenoloxidase) in hepatopancreas of E. carinicauda with 5 × 109 CFU/100 g diet were significantly higher than those in the control. Moreover, cumulative mortality (22.22%) post-challenge with A. hydrophila was the lowest in 5 × 109 CFU/100 g diet. The results suggested that Psychrobacter sp. B6 could effectively promote the growth, immunity, antioxidant capacity, and disease resistance of E. carinicauda. This study provided a reference for the study on the artificial breeding of E. carinicauda.

SnSe Nanosheet Array on Carbon Cloth as a High-Capacity Anode for Sodium-Ion Batteries.

Binder-free electrodes offer a great opportunity for developing high-performance sodium-ion batteries (SIBs) aiming at the application in energy storage devices. Tin selenide (SnSe) is considered to be a promising anode material for SIBs owing to its high theoretical capacity (780 mA h g-1). In this work, a SnSe nanosheet array (SnSe NS) on a carbon cloth is prepared using a vacuum thermal evaporation method. The as-prepared SnSe NS electrode does not have metal current collectors, binders, or any conductive additives. In comparison with the electrode of SnSe blocky particles (SnSe BP), the SnSe NS electrode delivers a higher initial charge capacity of 713 mA h g-1 at a current density of 0.1C and maintains a higher charge capacity of 410 mA h g-1 after 50 cycles. Furthermore, the electrochemical behaviors of the SnSe NS electrode are determined via pseudocapacitance and electrochemical impedance spectroscopy measurements, indicating a faster kinetic process of the SnSe NS electrode compared to that of the SnSe BP. Operando X-ray diffraction measurements prove that the SnSe NS exhibits better phase reversibility than the SnSe BP. After the cycles, the SnSe NS electrode still maintains its particular structure. This work provides a feasible method to prepare SnSe nanostructures with high capacity and improved sodium ion diffusion ability.

Highly efficient removal of aqueous Hg(II) by FeS micro-flakes.

FeS (mackinawite) is known to be effective in the sorption of aqueous Hg(II). However, FeS nanoparticles are apt to aggregate and easy to be oxidized, which limits their wide applications. Here, we have synthesized FeS micro-flakes which can be uniformly dispersed in water without aggregation. Owing to the good stability and dispersibility, FeS micro-flakes exhibit high efficiency in the removal of Hg(II) from water. The sorption of Hg(II) on the FeS micro-flakes is more consistent with the pseudo-second-order kinetic model and Langmuir model, indicating that the sorption of Hg(II) is mainly monolayer sorption dominated by chemical sorption. The maximum sorption capacity is 2680 mg/g at pH 5.6 and 30 °C, significantly higher than those of FeS nanoparticles and other Hg(II) scavengers. The pH studies indicate that FeS (0.31 g/L) can effectively remove >97.6 % of 200 mg/L Hg(II) in the pH range of 2-12 at 30 °C. Powder X-ray diffraction, elemental and sorption analyses suggest that Hg(II) is removed via chemical precipitation and surface adsorption. This study demonstrates the potential and viability of FeS micro-flakes for efficient removal of aqueous Hg(II).

Highly Sensitive Ratiometric Fluorescent Flexible Sensor Based on the RhB@ZIF-8@PVDF Mixed-Matrix Membrane for Broad-Spectrum Antibiotic Detection.

Antibiotics play an essential role in the treatment of various diseases. However, the overuse of antibiotics has led to the pollution of water bodies and food safety, affecting human health. Herein, we report a dual-emission MOF-based flexible sensor for the detection of antibiotics in water, which was prepared by first encapsulating rhodamine B (RhB) by a zeolite imidazolium ester skeleton (ZIF-8) and then blending it with polyvinylidene difluoride (PVDF). The luminescent properties, structural tunability, and flexible porosity of the MOF-based composites were combined with the processability and flexibility of polymers to prepare luminescent membranes. The sensor is capable of dual-emission ratiometric fluorescence sensing of nitrofurantoin (NFT) and oxytetracycline (OTC), exhibiting sensitive detection of fluorescence burst and fluorescence enhancement, respectively, with detection limits of 0.012 µM and 8.9 nM. With the advantages of visual detection, high sensitivity, short detection time, and simplicity, the highly sensitive ratiometric fluorescent flexible sensor has great potential for detecting antibiotics in an aqueous environment. It will further stimulate interest in luminescent MOF-based mixed matrix membranes and their sensing applications.