High-power 1018 nm Yb3+ doped fiber lasers with different YDF core and coiling diameters: theoretical and experimental study.

In this paper, the effect of the active fiber's core/cladding area ratio on the output parameters of 1018 nm fiber lasers has been investigated. In this regard, we conducted a comprehensive study of two fiber lasers that utilized 25/400 and 30/250 µm ytterbium-doped fibers (YDFs), both theoretically and experimentally. The optimum length of YDFs required for 40 dB of amplified spontaneous emission suppression was calculated. Theoretical studies also identified the YDF breaking zone for lengths greater than the optimum. The experimental results showed that selecting the proper dimensions and coiling diameter for the active fiber significantly increased the power and efficiency of the YDF laser. We obtained an output power of 943 W with a 75.5% slope efficiency for the co-pumped 30/250 µm YDFL which, to the best of our knowledge, is the highest reported value for the 1018 nm co-pumped fiber laser. An analysis of the experimental and theoretical results revealed that YDFs with a core/cladding area ratio greater than 1% are more suitable for realizing a high-power 1018 nm fiber laser. The findings of this study are crucial for the development of high-power 1018 nm fiber lasers with improved performance.

Chemical Compositions and Experimental and Computational Modeling of the Anticancer Effects of Cnidocyte Venoms of Jellyfish Cassiopea andromeda and Catostylus mosaicus on Human Adenocarcinoma A549 Cells.

Nowadays, major attention is being paid to curing different types of cancers and is focused on natural resources, including oceans and marine environments. Jellyfish are marine animals with the ability to utilize their venom in order to both feed and defend. Prior studies have displayed the anticancer capabilities of various jellyfish. Hence, we examined the anticancer features of the venom of Cassiopea andromeda and Catostylus mosaicus in an in vitro situation against the human pulmonary adenocarcinoma (A549) cancer cell line. The MTT assay demonstrated that both mentioned venoms have anti-tumoral ability in a dose-dependent manner. Western blot analysis proved that both venoms can increase some pro-apoptotic factors and reduce some anti-apoptotic molecules that lead to the inducing of apoptosis in A549 cells. GC/MS analysis demonstrated some compounds with biological effects, including anti-inflammatory, antioxidant and anti-cancer activities. Molecular docking and molecular dynamic showed the best position of each biologically active component on the different death receptors, which are involved in the process of apoptosis in A549 cells. Ultimately, this study has proven that both venoms of C. andromeda and C. mosaicus have the capability to suppress A549 cells in an in vitro condition and they might be utilized in order to design and develop brand new anticancer agents in the near future.

Closed-loop MOEMS accelerometer.

In this paper, a closed-loop micro-opto-electro-mechanical system (MOEMS) accelerometer based on the Fabry-Pérot (FP) interferometer is presented. The FP cavity is formed between the end of a cleaved single-mode optical fiber and the cross-section of a proof mass (PM) which is suspended by four U-shaped springs. The applied acceleration tends to move the PM in the opposite direction. The arrays of fixed and movable comb fingers produce an electrostatic force which keeps the PM in its resting position. The voltage that can provide this electrostatic force is considered as the output of the sensor. Using a closed-loop detection method it is possible to increase the measurement range without losing the resolution. The proposed sensor is fabricated on a silicon-on-insulator wafer using the bulk micromachining method. The results of the sensor characterization show that the accelerometer has a linear response in the range of ±5 g. In the closed-loop mode, the sensitivity and bias instability of the sensor are 1.16 V/g and 40 µg, respectively.

An especial configuration for an Nd:YAG end-pumped zigzag slab gain module based on crystalline polytetrafluoroethylene and a specific optical system for pump-beam delivery.

This work experimentally introduces a new single-crystal Nd:YAG end-pumped zigzag slab (EPZS) gain module based on a thin polytetrafluoroethylene (PTFE) sheet as a total internal reflection (TIR) protection layer. In this gain module, a specific optical pump-beam delivery (OPBD) system is used to improve the output beam quality (BQ) of the slab amplifier from 9.4 in a typical OPBD system to 4.7 with the total pump power of 2400 W. The average logarithmic small-signal gain (g0l) in the 0.06% Nd:YAG EPZS gain module at the laser beam incident angle of 7.1° was obtained around 0.64 for the two OPBD systems mentioned above.

Profilometry of an optical microfiber based on modal evolution.

The waist diameter of a tapered optical fiber (TOF) has been determined using the modal evolution during the tapering process of a single-mode optical fiber (SMF28) through the short-time Fourier transform (STFT) analysis. The STFT was utilized to calculate the cutoff moment of the different modes. By the knowledge of the cutoff diameter, the final diameter of the waist with accuracy better than 5 nm was measured. The TOF shape depends on the flame parameters, the material properties, and the stretching conditions. By calculating the TOF deformation rate of the TOF, the diameter of TOFs near the waist has been measured with an accuracy of 6.1%; moreover, the TOFs were fabricated with a non-uniform flame.

Optical Imaging Approaches to Monitor Static and Dynamic Cell-on-Chip Platforms: A Tutorial Review.

Miniaturized laboratories on chip platforms play an important role in handling life sciences studies. The platforms may contain static or dynamic biological cells. Examples are a fixed medium of an organ-on-a-chip and individual cells moving in a microfluidic channel, respectively. Due to feasibility of control or investigation and ethical implications of live targets, both static and dynamic cell-on-chip platforms promise various applications in biology. To extract necessary information from the experiments, the demand for direct monitoring is rapidly increasing. Among different microscopy methods, optical imaging is a straightforward choice. Considering light interaction with biological agents, imaging signals may be generated as a result of scattering or emission effects from a sample. Thus, optical imaging techniques could be categorized into scattering-based and emission-based techniques. In this review, various optical imaging approaches used in monitoring static and dynamic platforms are introduced along with their optical systems, advantages, challenges, and applications. This review may help biologists to find a suitable imaging technique for different cell-on-chip studies and might also be useful for the people who are going to develop optical imaging systems in life sciences studies.

Three-dimensional imaging through scattering media using a single pixel detector.

Imaging objects hidden by turbid media, such as smoke, fog, or biological tissues, is a challenge for scientists. Compressive ghost imaging and a photometric stereo approach are employed to estimate the 3D shape of an object behind a scattering medium. A sequence of speckle patterns is projected onto the object. Four images with different shadings are accurately reconstructed from the object behind a diffuser. The 3D shape is obtained by applying the photometric stereo to the reconstructed 2D images. This technique is robust against scattering, even for 3D imaging in opaque media with multiple scattering, and it is not sensitive to changes in the scattering media or displacement of the medium. Also, it has the benefits of compressive ghost imaging strategy, such as hyperspectral or polarimetric imaging with sub-Nyquist sampling. As a proof for this concept, the 3D shape of a target behind a diffuser was experimentally retrieved, and the results were compared with the original 3D shape and the 3D shape reconstructed in the absence of the diffuser. The accuracy of the reconstructed 3D shapes was maintained in the presence of the diffuser plate in various thicknesses and orientations.

High-Resolution FBG-Based Fiber-Optic Sensor with Temperature Compensation for PD Monitoring.

This paper presented a new sensor to detect and localize partial discharge (PD) in power transformers based on a fiber Bragg grating (FBG). The fundamental characteristics of the proposed sensor, as a PD detector, were temperature compensation and direction independence. The proposed high-resolution PD detector operated based on the FBG wavelength shift. It is necessary to evaluate the physical parameters of the sensor to achieve the best results. Therefore, in this paper, the detected signal strength was investigated for different angles and temperatures. A Teflon hollow mandrel and two FBGs attached to the inner and outer surfaces of the hollow mandrel were chosen as the inner transformer PD detector. The changes in the sensor output were less than 0.4 mV and 0.5 mV for direction variations and a temperature variation of 14 °C (degrees Celsius), respectively. Consequently, the proposed sensor could be successfully employed for the detection of a transformer PD signal.

Multimode Fabry⁻Perot Interferometer Probe Based on Vernier Effect for Enhanced Temperature Sensing.

New miniaturized sensors for biological and medical applications must be adapted to the measuring environments and they should provide a high measurement resolution to sense small changes. The Vernier effect is an effective way of magnifying the sensitivity of a device, allowing for higher resolution sensing. We applied this concept to the development of a small-size optical fiber Fabry⁻Perot interferometer probe that presents more than 60-fold higher sensitivity to temperature than the normal Fabry⁻Perot interferometer without the Vernier effect. This enables the sensor to reach higher temperature resolutions. The silica Fabry⁻Perot interferometer is created by focused ion beam milling of the end of a tapered multimode fiber. Multiple Fabry⁻Perot interferometers with shifted frequencies are generated in the cavity due to the presence of multiple modes. The reflection spectrum shows two main components in the Fast Fourier transform that give rise to the Vernier effect. The superposition of these components presents an enhancement of sensitivity to temperature. The same effect is also obtained by monitoring the reflection spectrum node without any filtering. A temperature sensitivity of -654 pm/°C was obtained between 30 °C and 120 °C, with an experimental resolution of 0.14 °C. Stability measurements are also reported.

Measurement of milli-Newton axial force and temperature using a hybrid microsilica sphere Fabry-Perot sensor.

An optical fiber sensor based on the Hybrid Fabry-Perot for simultaneous measurement of milli-Newton axial force and temperature is proposed. This structure is composed of a single-mode optical fiber (SMF) integrated to a silica capillary tube (SCT) with polydimethylsiloxane (PDMS). A microsilica sphere cavity (MSSC) is fabricated at the tip of a capillary tube. Consequently, an air gap followed by a microsilica sphere forms two cascade cavities. To explain the transferring load from the SMF to the SCT through PDMS, a shearing mechanism is employed. The experimental results show that axial force and temperature sensitivities of the air gap cavity in the range of 0-3.43 mN and 30-65°C are 170 pm/mN and 24 pm/°C, respectively, while the MSSC did not show any force sensitivity due to its rigidity. However, its temperature sensitivity is 34 pm/°C. The different sensitivities enable us to implement simultaneous sensing of force and temperature.

Investigation the cytotoxicity and photo-induced toxicity of carbon dot on yeast cell.

Carbon dots (CDs) as a new fluorescent material with excellent water solubility, chemical inertness, and easy surface modification are a good candidate for bioimaging and biosensing due to their low toxicity and good biocompatibility. Although carbon is not an intrinsically toxic substance, carbon nanomaterials such as CDs may cause risks to human health and the potentially hazardous effects of CDs on various living systems must be completely determined. So far, cytotoxicity studies of CDs have focused on human cells and are mainly conducted on limited cell lines. In the present study, toxicity assessment of CDs was evaluated on yeast cells Pichia pastoris as a unicellular eukaryotic model. Results revealed dose-dependent toxicity of CDs on yeast cells and less relative cell growth in 25 mg/ml of CDs as compared to the control group. CDs binding curve confirmed the interaction between CDs and surface of yeast cells. SEM images showed that the CDs caused cell shrinkage and hole formation on the surface of yeast cells and also induced slightly cell deformation. It was demonstrated that CDs could generate the ROS dose-dependently. Finally, results showed the growth inhibition and ROS generation effects of CDs were enhanced at light exposure, as an important environmental factor. These findings could have important implications for applications of CDs.

Integrating Optical Fiber Bridges in Microfluidic Devices to Create Multiple Excitation/Detection Points for Single Cell Analysis.

A microfluidic device is reported that employs an out-of-plane optical fiber bridge to generate two excitation and two detection spots in a microfluidic channel using only one excitation source and one detector. This fiber optic bridge was integrated into a single cell analysis device to detect an intact cell just prior to lysis and the injected lysate 2, 5, 10, or 15 mm downstream of the injection point. Using this setup the absolute migration times for analytes from cells stochastically entering the lysis intersection could be determined for the first time in an automated fashion. This allowed the evaluation of several separation parameters, including analyte band velocity, migration time drift, diffusion coefficient, injection plug length, separation efficiency (N), and plate height (H), which previously could only be estimated. To demonstrate the utility of this system, a peptide substrate for protein kinase B (PKB) was designed, synthesized, and loaded into T-lymphocytes in order to measure PKB activity in individual cells. The optical fiber bridge is easy to implement, inexpensive, and flexible in terms of changing the distances between the two detection points.

Microparticles manipulation and enhancement of their separation in pinched flow fractionation by insulator-based dielectrophoresis.

The separation and manipulation of microparticles in lab on a chip devices have importance in point of care diagnostic tools and analytical applications. The separation and sorting of particles from biological and clinical samples can be performed using active and passive techniques. In passive techniques, no external force is applied while in active techniques by applying external force (e.g. electrical), higher separation efficiency is obtained. In this article, passive (pinched flow fractionation) and active (insulator-based dielectrophoresis) methods were combined to increase the separation efficiency at lower voltages. First by simulation, appropriate values of geometry and applied voltages for better focusing, separation, and lower Joule heating were obtained. Separation of 1.5 and 6 µm polystyrene microparticles was experimentally obtained at optimized geometry and low total applied voltage (25 V). Also, the trajectory of 1.5 µm microparticles was controlled by adjusting the total applied voltage.

Focusing and continuous separation of microparticles by insulator-based dielectrophoresis (iDEP) in stair-shaped microchannel.

Focusing and separation of microparticles in a complex mixture have had wide applications in chemistry, biology, medicine, etc. This work presents a numerical and experimental investigation on focusing and continuous separation of microparticles in a geometrically optimized arrangement of steps in the form of a staircase using insulator-based dielectrophoresis (iDEP) mechanism. First, a detailed finite element analysis was performed on important parameters in the focusing and separation of microparticles, such as geometry of stair-shaped microchannel, total voltage, and voltage difference applied to reservoirs. The optimum parameters obtained from numerical analysis were used for experimental work. Theoretically, predicted microparticle trajectories are in good agreement with experimentally observed ones. Experimental and numerical results show that the performance of focusing of microparticles enhances with growth of the total voltage (in a constant voltage difference) and decreases with voltage difference. The fabricated iDEP microchip enhances the performance of focusing and separation of microparticles due to its stair-shaped microchannel and therefore operates at low DC total applied voltages of 90-110 V.

Real-time measurement of flow rate in microfluidic devices using a cantilever-based optofluidic sensor.

Real-time and accurate measurement of flow rate is an important reqirement in lab on a chip (LOC) and micro total analysis system (µTAS) applications. In this paper, we present an experimental and numerical investigation of a cantilever-based optofluidic flow sensor for this purpose. Two sensors with thin and thick cantilevers were fabricated by engraving a 2D pattern of cantilever/base on two polymethylmethacrylate (PMMA) slabs using a CO2 laser system and then casting a 2D pattern with polydimethylsiloxane (PDMS). The basic working principle of the sensor is the fringe shift of the Fabry-Pérot (FP) spectrum due to a changing flow rate. A Finite Element Method (FEM) is used to solve the three dimensional (3D) Navier-Stokes and structural deformation equations to simulate the pressure distribution, velocity and cantilever deflection results of the flow in the channel. The experimental results show that the thin and thick cantilevers have a minimum detectable flow change of 1.3 and 4 (µL min(-1)) respectively. In addition, a comparison of the numerical and experimental deflection of the cantilever has been done to obtain the effective Young's modulus of the thin and thick PDMS cantilevers.

Fabrication, characterization, and simulation of a cantilever-based airflow sensor integrated with optical fiber.

In this paper, we present the fabrication and packaging of a cantilever-based airflow sensor integrated with optical fiber. The sensor consists of a micro Fabry-Perot (FP) cavity including a fiber and a micro cantilever that is fabricated using the photolithography method. Airflow causes a small deflection of the micro cantilever and changes the cavity length of the FP, which makes the fringe shift. The pressure distribution and velocity streamlines across the cantilever resulted from the airflow in the channel have been simulated by the finite element method. The experimental results demonstrate that the sensor has a linear sensitivity of 190 [fringe shift (pm)] per (l/min) and a minimum detectable airflow change of 0.05 (l/min).

Localized modes in a defectless photonic crystal waveguide at terahertz frequencies.

We propose an idea to excite localized modes in a photonic crystal (PC) waveguide without ruining the discrete translational symmetry of the lattice. This can be done by arranging dispersive elements having negative permittivity over a desired frequency range into a periodic structure. We demonstrate numerically the realization of a cavity mode inside the air region of a geometrical defectless two-dimensional square-lattice PC consisting of polaritonic cylinders placed in air matrix. The corresponding waveguide structure in the form of a PC fiber supports the cavity mode as a guided mode to propagate along the guiding direction at very small propagation constant with near zero group velocity. These localized modes can be recognized as localized defectless modes inside the structure with four-fold symmetry.

Accuracy of peak-power compensation in fiber-guided and free-space acoustic-resolution photoacoustic microscopy.

Acoustic resolution photoacoustic microscopy (AR-PAM) has gained much attention in the past two decades due to its high contrast, scalable resolution, and relatively higher imaging depth. Multimode optical fibers (MMF) are extensively used to transfer light to AR-PAM imaging scan-head from the laser source. Typically, peak-power-compensation (PPC) is used to reduce the effect of pulse-to-pulse peak-power variation in generated photoacoustic (PA) signals. In MMF, the output intensity profile fluctuates due to the coherent nature of light and mode exchange caused by variations in the bending of the fibers during scanning. Therefore, using a photodiode (PD) to capture a portion of the total power of pulses as a measure of illuminated light on the sample may not be appropriate for accurate PPC. In this study, we have investigated the accuracy of PPC in fiber-guided and free-space AR-PAM systems. Experiments were conducted in the transparent and highly scattering medium. Based on obtained results for the MMF-based system, to apply PPC to the generated PA signals, tightly focused light confocal with the acoustic focus in a transparent medium must be used. In the clear medium and highly focused illumination, enhancement of about 45% was obtained in the homogeneity of an optically homogeneous sample image. In addition, it is shown that, as an alternative, free-space propagation of the laser pulses results in more accurate PPC in both transparent and highly scattering mediums. In free-space light transmission, enhancement of 25-75% was obtained in the homogeneity of the optically homogeneous sample image.

Acute morphine administration, morphine dependence, and naloxone-induced withdrawal syndrome affect the resting-state functional connectivity and Local Field Potentials of the rat prefrontal cortex.

Opiates are among the widely abused substances worldwide. Also, the clinical use of opioids can cause unwanted and potentially severe consequences such as developing tolerance and dependence. This study simultaneously measured the changes induced after morphine dependence and naloxone-induced withdrawal syndrome on the resting-state functional connectivity (rsFC) and Local Field Potential (LFP) power in the prefrontal cortex of the rat. The obtained results revealed that acute morphine administration significantly increased the LFP power in all frequency bands, as well as the rsFC strength of the prefrontal cortex, and naloxone injection reversed this effect. In contrast, chronic morphine administration reduced neural activity and general correlation values in intrinsic signals, as well as the LFP power in all frequency bands. In morphine-dependent rats, after each morphine administration, the LFP power in all frequency bands and the rsFC strength of the prefrontal cortex were increased, and these effects were further enhanced after naloxone precipitated withdrawal syndrome. The present study concludes that general correlation merely reflects the field activity of the local cortices imaged.

Method for tissue clearing: temporal tissue optical clearing.

Light absorption and scattering in biological tissue are significant variables in optical imaging technologies and regulating them enhances optical imaging quality. Optical clearing methods can decrease light scattering and improve optical imaging quality to some extent but owing to their limited efficacy and the potential influence of optical clearing agents on tissue functioning, complementing approaches must be investigated. In this paper, a new strategy of optical clearing proposed as time-dependent or temporal tissue optical clearing (TTOC) is described. The absorption and scattering in light interaction with tissue are regulated in the TTOC technique by altering the pulse width. Here, the dependence of optical properties of matter on the pulse width in a gelatin-based phantom was investigated experimentally. Then, a semi-classical model was introduced to computationally study of Ultra-short laser/matter interaction. After studying phantom, the absorption and scattering probabilities in the interaction of the pulse with modeled human skin tissue were investigated using the proposed model for pulse widths ranging from 1µs to 10fs. The propagation of the pulse through the skin tissue was simulated using the Monte Carlo technique by computing the pulse width-dependent optical properties (absorption coefficient µa, scattering coefficient µs, and anisotropy factor g). Finally, the penetration depth of light into the tissue and reflectance for different pulse widths was found.