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1.
Can Vet J ; 64(3): 257-261, 2023 03.
Artículo en Inglés | MEDLINE | ID: mdl-36874538

RESUMEN

A 12-year-old castrated male Cane Corso dog was presented with cervical swelling, lethargy, anorexia, and cough. An extensive neck mass with necrotic cysts was observed, severely adherent to surrounding tissues. Based on diagnostic imaging including ultrasound, computed tomography, and fine-needle aspiration cytology, paraesophageal abscess was tentatively diagnosed. However, after the mass was surgically removed, a diagnosis of thyroid carcinosarcoma composed of neoplastic cell populations with epithelial and mesenchymal origins was made via histopathology and immunohistochemistry. The dog died of a recurrent mass with pulmonary metastasis 105 d after surgery. This report describes a rare type of canine thyroid cancer, thyroid carcinosarcoma, preoperatively masquerading as an abscess and postoperatively confirmed by histopathology. Key clinical message: Thyroid carcinosarcoma, despite its rarity in dogs, should be included in the differential diagnoses of cervical mass especially with an aggressive progression.


Carcinosarcome thyroïdien déguisé en abcès paraoesophagien chez un chien Cane Corso. Un chien Cane Corso mâle castré de 12 ans a été présenté avec de l'enflure cervicale, de la léthargie, de l'anorexie et une toux. Une masse étendue du cou avec des kystes nécrotiques a été observée, adhérente fortement aux tissus environnants. Sur la base de l'imagerie diagnostique comprenant l'échographie, la tomodensitométrie et la cytologie par aspiration à l'aiguille fine, un abcès paraoesophagien a été provisoirement diagnostiqué. Cependant, après l'ablation chirurgicale de la masse, un diagnostic de carcinosarcome thyroïdien composé de populations de cellules néoplasiques d'origine épithéliale et mésenchymateuse a été posé par histopathologie et immunohistochimie. Le chien est décédé d'une masse récurrente avec métastase pulmonaire 105 jours après la chirurgie. Ce rapport décrit un type rare de cancer de la thyroïde canine, le carcinosarcome thyroïdien, se faisant passer pour un abcès en préopératoire et confirmé en postopératoire par histopathologie.Message clinique clé:Le carcinosarcome thyroïdien, malgré sa rareté chez le chien, doit être inclus dans les diagnostics différentiels de masse cervicale surtout à évolution agressive.(Traduit par Dr Serge Messier).


Asunto(s)
Carcinosarcoma , Enfermedades de los Perros , Neoplasias de la Tiroides , Masculino , Perros , Animales , Absceso/veterinaria , Bastones , Carcinosarcoma/veterinaria , Neoplasias de la Tiroides/veterinaria
2.
Can Vet J ; 63(6): 593-596, 2022 06.
Artículo en Inglés | MEDLINE | ID: mdl-35656522

RESUMEN

A 2.5-kg castrated male Maltese dog, suspected to be older than 10 y, was presented with a prolapsed mass at the anus. This had occurred on 2 previous occasions within the last 4 mo and had been managed with manual reduction and purse-string sutures. The rectal prolapse had viable tissue and was reducible but resulted in straining and fecal accumulation. Colopexy (with intracorporeal sutures) was performed laparoscopically using 3 ports; the distal colon was retracted cranially and attached to the abdominal wall with 3 simple interrupted sutures in a single row. The dog recovered uneventfully, had good appetite and normal activity, did not strain, and defecated without issues. There were no wound-healing complications and at 12-month post-operative examination, the patient was in good condition without clinical signs. Based on this case report, laparoscopic colopexy is clinically practical for management of rectal prolapse in small-breed dogs.


Colopexie laparoscopique pour prolapsus rectal récurrent chez un chien maltais. Un chien maltais mâle castré de 2,5 kg, suspecté d'avoir plus de 10 ans, a été présenté avec une masse faisant prolapsus à l'anus. Cela s'était produit à deux reprises au cours des quatre derniers mois et avait été géré avec une réduction manuelle et des sutures en bourse. Le prolapsus rectal avait des tissus viables et était réductible mais a entraîné des efforts et une accumulation fécale. La colopexie (avec sutures intracorporelles) a été réalisée par laparoscopie à l'aide de trois ouvertures; le côlon distal a été rétracté crânialement et attaché à la paroi abdominale avec trois sutures interrompues simples en une seule rangée. Le chien s'est rétabli sans incident, avait un bon appétit et une activité normale, ne s'est pas fatigué et a déféqué sans problème. Il n'y avait pas de complications de cicatrisation et lors de l'examen postopératoire de 12 mois, le patient était en bon état sans signes cliniques. Sur la base de ce rapport de cas, la colopexie laparoscopique est cliniquement pratique pour la gestion du prolapsus rectal chez les chiens de petite race.(Traduit par Dr Serge Messier).


Asunto(s)
Procedimientos Quirúrgicos del Sistema Digestivo , Enfermedades de los Perros , Laparoscopía , Prolapso Rectal , Animales , Colon/cirugía , Procedimientos Quirúrgicos del Sistema Digestivo/efectos adversos , Procedimientos Quirúrgicos del Sistema Digestivo/veterinaria , Enfermedades de los Perros/etiología , Enfermedades de los Perros/cirugía , Perros , Laparoscopía/métodos , Laparoscopía/veterinaria , Masculino , Prolapso Rectal/etiología , Prolapso Rectal/cirugía , Prolapso Rectal/veterinaria , Suturas/efectos adversos
3.
Can Vet J ; 60(10): 1104-1110, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31597997

RESUMEN

Two cases of surgical fenestration combined with omentalization for canine renal cysts using laparotomy and laparoscopy are described. After surgery, the cystic lesions gradually diminished in size, and a complete regression was confirmed in Case 2 by ultrasonography. The dogs maintained good condition without clinical signs of renal compromise for 14 months (Case 1) and 24 months (Case 2). Omentalization is a simple and effective procedure for canine renal cysts that conserves the remaining parenchyma and can be performed by a laparoscopic approach.


Fenestration chirurgicale combinée à une omentalisation pour le traitement de kystes rénaux chez deux chiens. Deux cas de fenestration chirurgicale combinée à une omentalisation pour traiter des kystes rénaux canins utilisant la laparotomie et la laparoscopie sont décrits. Après la chirurgie, les lésions kystiques ont graduellement diminué en grosseur, et une régression complète fut confirmée par échographie dans le Cas 2. Les chiens ont maintenu une bonne condition sans signe clinique de complication rénale pendant 14 mois (Cas 1) et 24 mois (Cas 2). L'omentalisation est une procédure simple et efficace pour les kystes rénaux canins qui conserve le parenchyme restant et peut être réalisée par une approche laparoscopique.(Traduit par Dr Serge Messier).


Asunto(s)
Quistes/veterinaria , Enfermedades de los Perros , Enfermedades Renales Quísticas/cirugía , Enfermedades Renales Quísticas/veterinaria , Laparoscopía/veterinaria , Animales , Perros , Laparotomía/veterinaria , Ultrasonografía
4.
Vet Surg ; 46(2): 226-232, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27990651

RESUMEN

OBJECTIVE: To investigate the feasibility of laparoscopic kidney biopsy with cup biopsy forceps in dogs (CupBF), and to compare to the use of a core biopsy needle (CoreBN). STUDY DESIGN: Experimental; randomized, controlled design. ANIMALS: Eight healthy, adult Beagle dogs. METHODS: Dogs were randomized to undergo laparoscopic biopsy of the right kidney using either 5 mm CupBF or a 16 gauge CoreBN. Intraoperative hemorrhage of the biopsy site was monitored. Biopsy quality was evaluated for tissue fragmentation and crushing, presence of renal cortex with or without medulla, and number of glomeruli. Postoperative packed cell volume, urinalysis, and ultrasonographic appearance of the biopsy site were evaluated. RESULTS: Biopsy specimens were obtained by both techniques and reliable hemostasis was achieved with direct compression in all dogs. The histologic score for CupBF biopsies was not significantly different from CoreBN biopsies. One CoreBN biopsy contained both renal cortex and medullar, while all CupBF biopsies contained cortex only. The mean (SD) number of glomeruli was significantly higher in CupBF biopsies [60 (9.1)] than CoreBN biopsies [26 (4.3)]. There was no gross hematuria, perirenal hematoma, or hydronephrosis in any dog postoperative. CONCLUSION: Laparoscopic kidney biopsy in dogs using 5 mm cup biopsy forceps is feasible with minimal risk and more glomeruli obtained compared to laparoscopic kidney biopsy using 16 gauge core biopsy needles.


Asunto(s)
Biopsia con Aguja Gruesa/veterinaria , Enfermedades de los Perros/diagnóstico , Enfermedades Renales/veterinaria , Animales , Biopsia con Aguja Gruesa/instrumentación , Enfermedades de los Perros/patología , Perros , Hemorragia/sangre , Hemorragia/prevención & control , Hemorragia/orina , Enfermedades Renales/diagnóstico , Laparoscopía , Masculino
5.
Vet Surg ; 46(1): 158-164, 2017 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-27898174

RESUMEN

OBJECTIVE: To report the surgical treatment of a pulmonary emphysematous cyst concurrent with primary pulmonary bronchoalveolar papillary carcinoma in a dog. STUDY DESIGN: Clinical case report. ANIMALS: 12-year-old 6.4 kg spayed female Shih Tzu dog. METHODS: The dog presented for surgical treatment of pulmonary emphysema. Radiography revealed that more than half of the left caudal lung lobe was enlarged and hyperlucent and computed tomography (CT) confirmed the presence of an emphysematous space. Thoracoscopic lung lobectomy was attempted but was converted to an intercostal thoracotomy due to poor visualization and pleural adhesions. A left caudal total lung lobectomy was performed using a self-cutting endoscopic stapler. RESULTS: The dog recovered uneventfully and a postoperative histopathologic diagnosis of pulmonary cystic bronchoalveolar papillary carcinoma was made. Re-evaluation using a CT scan with contrast study on postoperative days 27 and 177 revealed no evidence of residual, metastatic, or recurrent lesions. The dog has been doing well since surgery during the 11 month follow-up period. CONCLUSION: This case report suggests a potential relationship between pulmonary emphysematous diseases and primary lung tumors in dogs.


Asunto(s)
Neoplasias de los Bronquios/veterinaria , Carcinoma Papilar/veterinaria , Quistes/veterinaria , Enfermedades de los Perros/diagnóstico , Enfermedades Pulmonares/veterinaria , Animales , Neoplasias de los Bronquios/complicaciones , Neoplasias de los Bronquios/diagnóstico , Carcinoma Papilar/complicaciones , Carcinoma Papilar/diagnóstico , Quistes/complicaciones , Quistes/diagnóstico , Diagnóstico Diferencial , Enfermedades de los Perros/diagnóstico por imagen , Enfermedades de los Perros/cirugía , Perros , Enfisema/etiología , Enfisema/veterinaria , Femenino , Enfermedades Pulmonares/complicaciones , Enfermedades Pulmonares/diagnóstico , Toracotomía/veterinaria , Tomografía Computarizada por Rayos X/veterinaria
6.
J Zoo Wildl Med ; 48(3): 683-687, 2017 09.
Artículo en Inglés | MEDLINE | ID: mdl-28920812

RESUMEN

The purpose of this study was to establish reference ophthalmic findings in the common kestrel (Falco tinnunculus). Twenty healthy adult kestrels were included in this study. Ophthalmic examinations included slit lamp biomicroscopy, fundus exam, Schirmer tear test, conjunctival bacterial culture and isolation, corneal touch threshold, tonometry, and corneal diameter measurement. Mean tear production was 7.4 ± 3.27 mm/min, and mean intraocular pressure measured via applanation tonometry was 10.5 ± 3.15 mm Hg. In addition, the mean corneal touch threshold was 29.8 ± 20.1 mm, and the mean corneal diameter was 9.8 ± 1.1 mm. Of the 25 conjunctival swabs, 23 (92%) yielded bacterial growth. Most of these bacteria were gram positive (69.6%); the most predominant genus was Staphylococcus. This study presents reference values for ophthalmic examinations in common kestrels.


Asunto(s)
Falconiformes , Presión Intraocular/fisiología , Tonometría Ocular/veterinaria , Animales , Conjuntiva/microbiología , Córnea/fisiología , Femenino , Masculino , Examen Físico , Valores de Referencia , República de Corea
7.
Animals (Basel) ; 14(11)2024 Jun 05.
Artículo en Inglés | MEDLINE | ID: mdl-38891734

RESUMEN

A 2-year-old, intact female Pomeranian presented with bilateral forelimb lameness, characterized by the olecranon making contact with the ground. The patient experienced two separate incidents of falling, occurring four and three weeks before admission, respectively. Following each episode, non-weight-bearing lameness was initially observed in the left forelimb, followed by the development of crouch gait. Based on the physical examination, radiographic, and ultrasonographic findings, bilateral triceps brachii tendon disruption was diagnosed. Intraoperatively, excessive granulation tissue at the distal end of the tendon was excised. The footprint region of each triceps brachii tendon was decorticated with a high-speed burr until bleeding was observed. The triceps brachii tendon was reattached to completely cover its footprint on the olecranon using the Krackow suture technique. This method involves anchoring the suture through bone tunnels in the ulna. Trans-articular external skeletal fixation was applied to both forelimbs to immobile and stabilize the elbow joints for nine weeks. Subsequently, the dog gradually increased its walking activities while on a leash over a six-week period. At the three-year follow-up, the patient exhibited improved forelimb function and maintained a normal gait without signs of lameness. Suture-mediated anatomic footprint repair proved useful in this single case and may be an effective surgical alternative for the management of chronic triceps brachii tendon disruption in dogs.

8.
Front Vet Sci ; 11: 1270819, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38650855

RESUMEN

Two intact male dogs were evaluated for symptoms, including hematuria, prostatomegaly, anuria, lethargy, and abdominal mass. Presurgical evaluations, including complete physical examinations, blood examinations, abdominal radiography with contrast (only in Case 2), ultrasonography, and computed tomography and magnetic resonance imaging (only in Case 1), were performed. A paraprostatic cyst was diagnosed initially, and laparoscopic exploration and surgery were performed. Complete resection was performed in case 1, whereas partial resection with omentalization was performed in case 2. Histopathological examination of the tissue samples confirmed the presence of paraprostatic pseudocysts in both cases, with no evidence of an epithelial lining. These two cases represent the first documented instances of laparoscopic treatment for extraparenchymal prostatic cysts. The laparoscopic treatment proved feasible even in the case of a giant cyst causing anuria (Case 2). Paraprostatic cysts should be considered a potential differential diagnosis for abnormal urination accompanied by an abdominal mass, and long-term postoperative follow-up is necessary.

9.
Animals (Basel) ; 14(7)2024 Apr 03.
Artículo en Inglés | MEDLINE | ID: mdl-38612332

RESUMEN

Sinus venosus atrial septal defects (SVASDs), concurrent with partial anomalous pulmonary venous connections (PAPVCs), are a rare congenital heart disease in dogs. Surgical correction is essential when clinical signs or significant hemodynamic changes are present. We aimed to report on the successful surgical correction of an SVASD with PAPVCs, using a computed tomography (CT)-based customized 3D cardiac model. A 10-month-old male poodle was referred for corrective surgery for an ASD. Echocardiography confirmed a hemodynamically significant left-to-right shunting flow through an interatrial septal defect and severe right-sided heart volume overload. For a comprehensive diagnosis, a CT scan was performed, which confirmed an SVASD with PAPVCs. A customized 3D cardiac model was used for preoperative decision-making and surgical rehearsal. The defect was repaired using an autologous pericardial patch under a cardiopulmonary bypass (CPB). Temporary pacing was applied for sinus bradycardia and third-degree atrioventricular block. The patient recovered from the anesthesia without further complications. The pacemaker was removed during hospitalization and the patient was discharged without complications 2 weeks post-surgery. At the three-month follow-up, there was no shunting flow in the interatrial septum and the right-sided volume overload had been resolved. The cardiac medications were discontinued, and there were no complications. This report indicates the validity of surgical correction under CPB for an SVASD with PAPVCs, and the advantages of utilizing a CT-based 3D cardiac model for preoperative planning to increase the surgical success rate.

10.
Vet Ophthalmol ; 16(1): 43-6, 2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22519957

RESUMEN

OBJECTIVE: To determine the effect of 0.5% proparacaine in tonometry by evaluating corneal touch threshold (CTT) and intraocular pressure (IOP). ANIMAL STUDIED: Nine rats (18 eyes, Sprague-Dawley) and 10 dogs (20 eyes, Beagle) PROCEDURES: The IOP and CTT were measured in each eye before and after topical anesthesia with 0.5% proparacaine. The IOP was evaluated using Tonopen for dogs and Tonolab for rats. The corneal sensitivity was evaluated by CTT through a Cochet-Bonnet aesthesiometer. RESULTS: The mean IOP was not significantly changed in rats or dogs before and after topical anesthesia. However, after application of proparacaine, CTT was significantly increased in both animal groups compared with that before application of proparacaine. CONCLUSION: From this study, topical anesthesia was found to significantly lower the corneal sensitivity but have little effect on IOP measurements. In ophthalmologic examination, topical anesthesia can be used to reduce corneal sensation without an effect on IOP.


Asunto(s)
Anestesia/veterinaria , Córnea/efectos de los fármacos , Presión Intraocular/efectos de los fármacos , Tonometría Ocular/veterinaria , Anestesia/métodos , Anestésicos Locales/farmacología , Animales , Perros , Femenino , Umbral del Dolor/efectos de los fármacos , Propoxicaína/farmacología , Ratas , Ratas Sprague-Dawley
11.
Animals (Basel) ; 13(18)2023 Sep 14.
Artículo en Inglés | MEDLINE | ID: mdl-37760321

RESUMEN

Cor triatriatum dexter (CTD) is an uncommon congenital cardiac anomaly in dogs. This case report describes successful membranectomy for CTD via partial venous inflow occlusion under mild hypothermia in a dog. A 7-month-old intact male mixed-breed dog weighing 20.5 kg presented with a history of abdominal distention, lethargy, and anorexia. Clinical examination, radiography, echocardiography, microbubble testing, and computed tomography revealed a remnant right atrium membrane obscuring the venous blood inflow from the vena cava. Considering the potential risk of re-stenosis following interventional treatment, curative resection involving surgical membranectomy via venous inflow occlusion was performed. By performing partial venous inflow occlusion under mild hypothermia (34.5 °C), sufficient time was obtained to explore the defect and resect the remnant membrane. The dog recovered without any complications, and the clinical signs were relieved. This case illustrates that partial venous inflow occlusion under mild hypothermia is feasible for achieving curative resection of cor triatriatum dexter in dogs.

12.
J Vet Med Sci ; 84(6): 799-803, 2022 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-35431283

RESUMEN

A 10-year-old intact male Shih Tzu dog presented with hematuria. Double-contrast cystography revealed a polypoid filling defect at the apex of the urinary bladder. Ultrasonography revealed a heterogeneously hypoechoic intramural mass with minimal vascular flow beneath the submucosal layer. After partial cystectomy, a well-demarcated bladder leiomyosarcoma was diagnosed on histopathology. The patient was alive and well without any clinical signs, recurrence, or metastasis at the 29-month follow-up after the surgical removal of the bladder mass. Leiomyosarcoma should be considered as a differential diagnosis if mass-like lesions are observed in the urinary bladder, although this type of malignancy is rare in canines. Histopathological confirmation is important for predicting prognosis and determining further medical plans.


Asunto(s)
Enfermedades de los Perros , Leiomiosarcoma , Neoplasias de la Vejiga Urinaria , Animales , Cistectomía/veterinaria , Enfermedades de los Perros/diagnóstico por imagen , Enfermedades de los Perros/cirugía , Perros , Hematuria/etiología , Hematuria/veterinaria , Leiomiosarcoma/diagnóstico por imagen , Leiomiosarcoma/cirugía , Leiomiosarcoma/veterinaria , Masculino , Vejiga Urinaria/patología , Neoplasias de la Vejiga Urinaria/diagnóstico por imagen , Neoplasias de la Vejiga Urinaria/cirugía , Neoplasias de la Vejiga Urinaria/veterinaria
13.
Vet Sci ; 9(5)2022 May 12.
Artículo en Inglés | MEDLINE | ID: mdl-35622762

RESUMEN

Tumors of sweat glands usually originate from apocrine glands and can develop throughout the body but are rare in dogs. This report describes the retroperitoneal metastasis of primary cutaneous apocrine adenocarcinoma. An 8-year-old, spayed female beagle dog, weighing 11.7 kg, presented with a history of anorexia, hypodynamia, and weight loss. Clinical examination, radiography, ultrasonography, and computed tomography revealed a skin mass on the dorsum of the right metatarsal region, an enlarged ipsilateral popliteal lymph node, and a retroperitoneal mass. Fine-needle aspiration cytology of the popliteal lymph node suggested metastasis of an apocrine sweat gland tumor. Surgical excision of the skin mass, popliteal lymph node, and retroperitoneal mass was performed. The retroperitoneal mass was diagnosed as a metastasis of primary cutaneous apocrine adenocarcinoma. Immunohistochemistry revealed that the tumor cells were positive for cytokeratin 7 but negative for cytokeratin 20 and S100 proteins. There were no postoperative complications, except for temporary hindlimb edema, including local recurrence or metastasis, in the 6-month postoperative follow-up period. This case illustrates that although malignant apocrine gland tumors are rare in dogs, a wide resection of primary cutaneous apocrine gland adenocarcinomas is recommended because of the risk of local invasion or distant metastasis.

14.
Vet Sci ; 9(12)2022 Dec 14.
Artículo en Inglés | MEDLINE | ID: mdl-36548855

RESUMEN

Surgical ligation of a patent ductus arteriosus is regarded as a standard treatment approach with a low complication rate if performed by experienced surgeons, and it has been performed successfully for decades in dogs. However, there are no reports describing the clinical symptoms related to granulomatous inflammation after the surgical ligation of the patent ductus arteriosus. This report describes the clinical symptoms caused by granulomatous inflammation and subacute pericarditis in a dog that had undergone the surgical closure of a patent ductus arteriosus 2 years previously. Exploratory thoracotomy was performed for subtotal pericardiectomy, and a biopsy was performed to obtain specimens for histopathological examination and culture tests. The clinical symptoms were relieved after surgery. The persistent leukocytosis improved with steroid administration. This case illustrates that the granulomatous inflammatory response associated with silk suture granuloma is a rare postoperative complication of ductal ligation. In such cases, pericardiectomy can help relieve the clinical symptoms.

15.
Am J Vet Res ; 72(1): 42-50, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21194334

RESUMEN

OBJECTIVE: To establish the dose-dependent effects of high-molecular-weight hyaluronic acid (HA) supplementation on chondrogenesis by mesenchymal stem cells (MSCs) cultured on chitosan sponges and to determine the extent to which MSC matrix production (chondrogenesis) can be influenced by incorporation of high-molecular-weight HA into chitosan scaffolds. SAMPLE POPULATION: Murine MSCs derived from a multipotent bone marrow stromal precursor. PROCEDURES: MSCs were seeded on chitosan and chitosan-HA scaffolds in chondrogenic medium with various HA concentrations. Scanning electron microscopy, fluorescence microscopy (viability assay), and DNA quantification were used to assess cell attachment, distribution, and viability 48 hours after seeding. Constructs were cultured for 3 weeks prior to evaluation of cell distribution and chondrogenic differentiation via histologic evaluation and quantification of DNA, glycosaminoglycan, and collagen II. RESULTS: 48 hours after MSC seeding, cell viability and DNA content were similar among groups. Three weeks after seeding, HA supplementation of the culture medium improved matrix production in a dose-dependent manner, as indicated by matrix glycosaminoglycan and collagen II concentrations. The scaffold composition, however, had no significant effect on matrix production. CONCLUSIONS AND CLINICAL RELEVANCE: High-molecular-weight HA supplementation in culture medium had a dose-dependent effect on matrix production and thus chondrogenic differentiation of MSCs cultured on chitosan sponges. The addition of HA in the surrounding fluid during chondrogenesis should improve cartilage production and may be useful for producing engineered cartilage tissues.


Asunto(s)
Células de la Médula Ósea/fisiología , Quitosano/química , Condrogénesis/fisiología , Ácido Hialurónico/metabolismo , Células Madre Mesenquimatosas/fisiología , Ingeniería de Tejidos/instrumentación , Animales , Células de la Médula Ósea/ultraestructura , Supervivencia Celular , Células Madre Mesenquimatosas/ultraestructura , Ratones , Ingeniería de Tejidos/métodos
16.
Vet Sci ; 8(10)2021 Oct 12.
Artículo en Inglés | MEDLINE | ID: mdl-34679055

RESUMEN

Symphyseal distraction osteotomy (SDO) with a polymethyl methacrylate (PMMA) spacer is an effective surgical treatment for cats with pelvic stenosis. This study reports the successful treatment of urethral obstruction due to ischiocavernosus muscle (IM) tension after SDO with a PMMA spacer. A 2-year-old castrated male Korean domestic shorthair feline had megacolon and pelvic canal stenosis. The ratio of the maximal diameter of the colon to the L5 length and the pelvic canal diameter ratio were 1.6 and 0.45, respectively. Pelvic SDO was performed with a PMMA spacer, leading to pelvic canal enlargement (pelvic canal diameter ratio: 0.73). Two days after surgery, dysuria was identified immediately after removing the preoperatively placed urinary catheter. Complete blood counts and serum biochemical profiles were within the reference intervals, and a positive contrast retrograde urethrogram confirmed urethral obstruction at the level of the membranous-pelvic urethra region. Increased tension of the IM leading to a narrowed urethra was suspected as the cause of urethral obstruction. After IM release at the level of origin on the ischium, the patient had an uneventful recovery with spontaneous urination. Muscle release resulted in excellent functional restoration, with no intraoperative or postoperative complications reported during the 12-month long-term follow-up. Therefore, SDO with IM release could be a feasible therapeutic option for severe pelvic stenosis without complications, such as urethral obstruction, in cats.

17.
Vet Sci ; 8(3)2021 02 28.
Artículo en Inglés | MEDLINE | ID: mdl-33671051

RESUMEN

Corrective osteotomy has been applied to realign and stabilize the bones of dogs with lameness. However, corrective osteotomy for angular deformities requires substantial surgical experience for planning and performing accurate osteotomy. Three-dimensional printed patient-specific guides (3D-PSGs) were developed to overcome perioperative difficulties. In addition, novices can easily use these guides for performing accurate corrective osteotomy. We compared the postoperative results of corrective osteotomy accuracy when using 3D-PSGs in dogs between novice and experienced surgeons. We included eight dogs who underwent corrective osteotomy: three angular deformities of the radius and ulna, three distal femoral osteotomies, one center of rotational angle-based leveling osteotomy, and one corrective osteotomy with stifle arthrodesis. All processes, including 3D bone modeling, production of PSGs, and rehearsal surgery were carried out with computer-aided design software and a 3D-printed bone model. Pre- and postoperative positions following 3D reconstruction were evaluated by radiographs using the 2D/3D registration technique. All patients showed clinical improvement with satisfactory alignment and position. Postoperative accuracy evaluation revealed no significant difference between novice and experienced surgeons. PSGs are thought to be useful for novice surgeons to accurately perform corrective osteotomy in dogs without complications.

18.
J Mater Sci Mater Med ; 21(8): 2479-90, 2010 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-20499139

RESUMEN

The biocompatibility and biomimetic properties of chitosan make it attractive for tissue engineering but its use is limited by its cell adhesion properties. Our objectives were to produce and characterize chitosan and reacetylated-chitosan fibrous scaffolds coated with type II collagen and to evaluate the effect of these chemical modifications on mesenchymal stem cell (MSC) adhesion. Chitosan and reacetylated-chitosan scaffolds obtained by a wet spinning method were coated with type II collagen. Scaffolds were characterized prior to seeding with MSCs. The constructs were analyzed for cell binding kinetics, numbers, distribution and viability. Cell attachment and distribution were improved on chitosan coated with type II collagen. MSCs adhered less to reacetylated-chitosan and collagen coating did not improve MSCs attachment on those scaffolds. These findings are promising and encourage the evaluation of the differentiation of MSCs in collagen-coated chitosan scaffolds. However, the decreased cell adhesion on reacetylated chitosan scaffold seems difficult to overcome and will limit its use for tissue engineering.


Asunto(s)
Quitosano/química , Quitosano/farmacología , Colágeno Tipo II/farmacología , Células Madre Mesenquimatosas/efectos de los fármacos , Andamios del Tejido , Acetilación , Animales , Adhesión Celular/efectos de los fármacos , Técnicas de Cultivo de Célula/métodos , Células Cultivadas , Materiales Biocompatibles Revestidos/química , Materiales Biocompatibles Revestidos/farmacología , Colágeno Tipo II/química , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/fisiología , Ratones , Microscopía Electrónica de Rastreo , Microscopía Electrónica de Transmisión , Porosidad , Espectroscopía Infrarroja por Transformada de Fourier , Andamios del Tejido/química , Agua/farmacología
19.
Vet Ophthalmol ; 13(2): 122-5, 2010 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-20447032

RESUMEN

A 10-year-old intact male Yorkshire Terrier was referred for investigation of a large raised and nonpainful corneal lesion oculus dexter. Clinical examination revealed a pale, translucent corneal mass, which occupied half of the corneal surface and measured 11 mm x 11 mm x 13 mm. The mass was removed by superficial keratectomy and equine amniotic membrane (AM) was transplanted into the large corneal defect to cover the wound and provide tectonic support for the remaining cornea. The mass was histologically confirmed as a corneal epithelial inclusion cyst. There was no evidence of recurrence or complication at the surgical site 100 days postoperatively. Corneal epithelial inclusion cysts are uncommon in dogs. Although superficial keratectomy is the recommended treatment for corneal inclusion cyst, the combination of superficial keratectomy and AM transplantation had to be considered as an alternative for repair of large corneal defects. This is the first case report of the combined application of AM and superficial keratectomy to successfully treat a corneal inclusion cyst in a dog.


Asunto(s)
Amnios/trasplante , Enfermedades de la Córnea/cirugía , Quistes/veterinaria , Enfermedades de los Perros/cirugía , Epitelio Corneal/cirugía , Animales , Quistes/terapia , Perros , Epitelio Corneal/patología , Caballos , Masculino
20.
J Vet Med Sci ; 82(11): 1602-1606, 2020 Nov 12.
Artículo en Inglés | MEDLINE | ID: mdl-32801281

RESUMEN

A 13-year-old, 5.6-kg castrated-male Maltese was presented for reverse sneezing. A dome-shaped round mass abutting diaphragm was incidentally found ventral to caudal vena cava, which had the same echogenicity and density as that of the liver during ultrasonography and computed tomography, showing isoattenuation with a contrast study. Vascular distribution was identified throughout the mass. A caval foramen hernia (CFH) was diagnosed tentatively, followed by a herniorrhaphy and splenectomy of the chronically congested spleen. The patient had been doing well for 5-month postoperative but died because of aspiration pneumonia. CFH is an extremely rare condition, requiring surgery due to compression of the vena cava. It should be considered as a differential diagnosis when intrathoracic, mass-like lesions are identified near the diaphragm.


Asunto(s)
Enfermedades de los Perros , Hernia Diafragmática , Animales , Enfermedades de los Perros/diagnóstico por imagen , Enfermedades de los Perros/cirugía , Perros , Hernia Diafragmática/veterinaria , Hígado , Masculino , Vena Cava Inferior , Venas Cavas
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