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1.
ScientificWorldJournal ; 2015: 764905, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25879070

RESUMEN

BACKGROUND: The etiology of dentine hypersensitivity (DH) is still inconclusive and there are few studies concerning it in children. AIM: To evaluate clinical, dietary, and salivary variables in children with DH complaints. DESIGN: Forty-eight children were asked about DH. Data regarding dietary habits were collected from the children's parents and an examination was performed to determine dental erosion. Dental biofilm was estimated by oral hygiene status, according to Greene and Vermillion's Simplified Oral Hygiene Index (OHI-S). Whole saliva was collected under mechanical stimulation and evaluated salivary flow rate, initial pH, buffer capacity, and calcium and phosphate concentrations. The temperature of soft drinks, drinking method, sense of bitter taste, and other variables were also determined. Possible factors associated with DH were analyzed by univariate and multiple Poisson regression analyses. The prevalence ratio (PR) values and 95% confidence intervals (95% CI) were calculated. RESULTS: DH was associated with the presence of dental erosion (PR; 95% CI = 2.23; 1.05 to 4.71) and salivary flow rate (2.49; 1.05 to 5.91). When the presence of erosion was not included, other variables were retained as follows: bitter taste (2.36; 1.38 to 4.03), OHI-S (0.47; 0.23 to 0.97). CONCLUSION: DH in children is associated with factors related to dental erosion.


Asunto(s)
Sensibilidad de la Dentina/diagnóstico , Sensibilidad de la Dentina/etiología , Conducta Alimentaria/fisiología , Saliva/fisiología , Erosión de los Dientes/diagnóstico , Erosión de los Dientes/etiología , Niño , Preescolar , Estudios Transversales , Femenino , Humanos , Masculino , Higiene Bucal/efectos adversos , Higiene Bucal/métodos
2.
Braz Oral Res ; 34: e115, 2020 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-32901730

RESUMEN

The aim of the present transversal study was to evaluate the clinical and biochemical salivary parameters of children with and without erosive tooth wear (ETW). The study population was children aged 4 to 9 years. A trained and calibrated examiner (kappa value for intraexaminer reliability = 0.89) classified the children into ETW (n = 24) and control groups (n = 24), and applied the O'Brien index. The salivary flow rate was initially evaluated by stimulated sialometry (paraffin chewing). Afterwards, the collected saliva was submitted to biochemical analyses of pH, uric acid, total buffering capacity, ferric-reducing antioxidant power, reduced glutathione, calcium, and phosphorus. Among the ETW children, 20 (83%) had dental lesions restricted to enamel, and 4 (17%) presented lesions affecting both enamel and dentin. A statistically significant difference between the groups was obtained only for the pH values (t-test; p = 0.004), with averages of 7.31 and 7.56 for the control and the ETW groups, respectively. Considering the parameters evaluated in general, it is suggested that the salivary profile of children with ETW does not differ considerably from that of children without ETW. However, the pH mean value seems to be slightly higher in ETW children, but is still within the normal physiological range.


Asunto(s)
Desgaste de los Dientes , Calcio , Niño , Preescolar , Esmalte Dental , Humanos , Saliva , Erosión de los Dientes
3.
Oral Health Prev Dent ; 5(1): 73-8, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17366764

RESUMEN

PURPOSE: This study measured the flow rate, pH and buffering capacity of saliva from well- and poorly metabolically controlled Type 2 diabetic patients in three cities of the southern part of Brazil, compared with healthy individuals from the same cities. MATERIALS AND METHODS: Whole saliva was collected by mechanical stimulation and buffering capacity and glucose level were measured. Blood was collected after 12 hours fasting and glucose and glycosylated haemoglobin concentrations were determined. The data were analysed by one-way ANOVA and Student-Newman-Keuls (alpha= 0.05). RESULTS: The flow rate was lower in the Type 2 diabetic patients, regardless of whether they were well or poorly metabolically controlled, compared with healthy individuals (p < 0.05). Salivary glucose concentration was higher in both diabetic patient groups, i.e. well and poorly metabolically controlled, than in the control (p < 0.05). CONCLUSION: The metabolic control of hyperglycaemia was not sufficient to improve the salivary flow rate or the salivary glucose concentration.


Asunto(s)
Diabetes Mellitus Tipo 2/fisiopatología , Saliva/fisiología , Adulto , Anciano , Anciano de 80 o más Años , Glucemia/análisis , Tampones (Química) , Diabetes Mellitus Tipo 2/sangre , Diabetes Mellitus Tipo 2/prevención & control , Ayuno , Femenino , Glucosa/análisis , Hemoglobina Glucada/análisis , Humanos , Concentración de Iones de Hidrógeno , Hiperglucemia/sangre , Hiperglucemia/prevención & control , Masculino , Persona de Mediana Edad , Saliva/química , Saliva/metabolismo , Tasa de Secreción/fisiología , Xerostomía/fisiopatología
4.
Artículo en Inglés | MEDLINE | ID: mdl-26868468

RESUMEN

OBJECTIVE: To evaluate the salivary parameters of individuals with juvenile idiopathic arthritis (JIA) and determine if there is a correlation with the simplified oral hygiene index (OHI-S). STUDY DESIGN: Stimulated whole saliva was collected from children, aged 6 to 12 years, with JIA (n = 36) and compared with saliva from a control group (CG) composed of healthy children matched by demographic characteristics (n = 36). Salivary parameters evaluated were amylase and peroxidase activities, calcium, phosphate, and total sialic acid concentration. Data were compared by two-tailed Student's t test, and salivary parameters were evaluated for correlations with OHI-S by Spearman's correlation. RESULTS: The JIA group had a statistically significant reduction in all salivary parameters: α-amylase (20%) (CG, 9.84 ± 4.23; JIA 4.80 ± 3.10) and peroxidase (55%) (CG, 35.05 ± 14.97; JIA, 15.78 ± 6.44) activities, total sialic acid (25%) (CG, 0.024 ± 0.01; JIA, 0.010 ± 0.04), calcium (13%) (CG, 6.26 ± 2.26; JIA, 5.44 ± 2.26) and phosphate (50%) (CG, 10.88 ± 1.49; JIA, 5.45 ± 0.88) concentration compared with the CG (P ≤ .05). There were no significant correlations between the salivary parameters and OHI-S in either group. CONCLUSION: JIA patients showed a reduction of different salivary parameters involved in maintaining oral health.


Asunto(s)
Artritis Juvenil/complicaciones , Saliva/química , Brasil , Estudios de Casos y Controles , Niño , Femenino , Humanos , Masculino , Índice de Higiene Oral
5.
Arch Oral Biol ; 60(11): 1650-4, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-26351748

RESUMEN

OBJECTIVE: It is recognized that cystic fibrosis (CF) patients present a risk for oral diseases, since it affects exocrine glands, and the treatment consists of a carbohydrate-rich diet. Recognizing the protective function of saliva on maintaining oral health, the aim of the study was to evaluate salivary parameters in stimulated whole saliva from children with CF. METHODS: A case-control study was conducted comparing stimulated whole saliva of healthy (n=28; control group) and CF children (n=21; experimental group). Salivary flow rate, initial pH, buffer capacity (total and in each range of pH), total protein and sialic acid (total, free, and conjugated) concentration, α-amylase and salivary peroxidase activities were evaluated. Data were compared by two-tailed Student t test (95% CI; p ≤ 0.05). RESULTS: CF patients presented a significant reduction in salivary parameters compared with the control group (p ≤ 0.05): salivary flow rate (36%), buffer capacity (pH range from 6.9 to 6.0), sialic acid concentration (total 75%, free 61%, and conjugated 83%); α-amylase and salivary peroxidase activities (55%). Additionally, a significant increase in total protein concentration (180%) of stimulated whole saliva from CF patients was verified compared with the control group (p ≤ 0.05). CONCLUSION: Children with CF presented significant changes in salivary composition, including salivary flow rate, buffering capacity and protective proteins of the oral cavity, compared with children without CF.


Asunto(s)
Fibrosis Quística/fisiopatología , Saliva/metabolismo , Proteínas y Péptidos Salivales/metabolismo , Tampones (Química) , Estudios de Casos y Controles , Niño , Femenino , Humanos , Concentración de Iones de Hidrógeno , Masculino , Ácido N-Acetilneuramínico/análisis , Ácido N-Acetilneuramínico/metabolismo , Peroxidasa/análisis , Peroxidasa/metabolismo , Saliva/química , Proteínas y Péptidos Salivales/análisis , Tasa de Secreción/fisiología , alfa-Amilasas/análisis , alfa-Amilasas/metabolismo
6.
Braz. oral res. (Online) ; 34: e115, 2020. tab
Artículo en Inglés | LILACS, BBO - odontología (Brasil) | ID: biblio-1132663

RESUMEN

Abstract The aim of the present transversal study was to evaluate the clinical and biochemical salivary parameters of children with and without erosive tooth wear (ETW). The study population was children aged 4 to 9 years. A trained and calibrated examiner (kappa value for intraexaminer reliability = 0.89) classified the children into ETW (n = 24) and control groups (n = 24), and applied the O'Brien index. The salivary flow rate was initially evaluated by stimulated sialometry (paraffin chewing). Afterwards, the collected saliva was submitted to biochemical analyses of pH, uric acid, total buffering capacity, ferric-reducing antioxidant power, reduced glutathione, calcium, and phosphorus. Among the ETW children, 20 (83%) had dental lesions restricted to enamel, and 4 (17%) presented lesions affecting both enamel and dentin. A statistically significant difference between the groups was obtained only for the pH values (t-test; p = 0.004), with averages of 7.31 and 7.56 for the control and the ETW groups, respectively. Considering the parameters evaluated in general, it is suggested that the salivary profile of children with ETW does not differ considerably from that of children without ETW. However, the pH mean value seems to be slightly higher in ETW children, but is still within the normal physiological range.


Asunto(s)
Humanos , Preescolar , Niño , Desgaste de los Dientes , Saliva , Erosión de los Dientes , Calcio , Esmalte Dental
7.
Arq. odontol ; 56: 1-7, jan.-dez. 2020. tab
Artículo en Portugués | LILACS, BBO - odontología (Brasil) | ID: biblio-1120471

RESUMEN

Objetivo: Avaliar a alteração da rugosidade superficial de uma resina composta nanoparticulada (Filtek Z350 XT ® ) após o uso de produtos clareadores de autoaplicação contento baixa concentração de peróxido de hidrogênio. Métodos: Para isto, foram confeccionados 30 corpos de prova deste material, divididos aleatoriamente em 3 grupos com 10 amostras cada, da seguinte forma: G1 (grupo controle) no qual as amostras não foram submetidas à ação de nenhum produto clareador; G2, realização de procedimento clareador com peróxido de hidrogênio a 10% (Crest 3D White Professional Effects Whitestrips ® ) em 2 aplicações de 30 minutos, por 10 dias consecutivos; e G3, tratamento com peróxido de hidrogênio a 7,7% (pincel Pretty Smile ® ) com o mesmo número de aplicações, tempo e dias do G2. Após este período cada corpo de prova foi analisado no rugosímetro Surftest SJ-301, para determinar a sua rugosidade superficial média. Estes dados foram então submetidos à análise estatística por meio da análise de variância (ANOVA) e teste de Tukey, nível de significância de 5% para comparações múltiplas. Resultados: Ao final do experimento, houve diferenças estatísticas significantes entre o grupo controle (G1) e os demais grupos (G2 e G3), com p < 0,05. Porém, quando analisados apenas os grupos submetidos ao clareamento com o peróxido de hidrogênio em diferentes concentrações (G2 e G3), não foi encontrada diferença significativa (p > 0,05). Conclusão: Após 10 dias, os produtos clareadores testados determinaram aumento significativo nos valores de rugosidade superficial média da resina composta nanoparticulada.


Aim: Tod evaluate the change in surface roughness of a nanoparticulate composite resin (Filtek Z350 XT ® ) after using over-the-counter bleaching products, containing a low concentration of hydrogen peroxide. Methods: For this, 30 specimens of this material were made, randomly divided into 3 groups with 10 samples each, as follows: G1 (control group) in which the samples were not subjected to the action of any bleaching product; G2, performing a bleaching procedure with 10% hydrogen peroxide (Crest 3D White Professional Effects Whitestrips ® ), in 2 applications of 30 minutes, for 10 consecutive days; and G3, treatment with 7.7% hydrogen peroxide (Pretty Smile ® ), with the same number of applications, time, and days as G2. After this period, each specimen was analyzed, using the Surftest SJ-301, to determine its average surface roughness. These data were then subjected to statistical analysis through analysis of variance (ANOVA) and Tukey's Test, with a significance level of 5% for multiple comparisons. Results: Statistically significant differences were found between the control group (G1) and the other groups (G2 and G3), with p < 0.05. However, when analyzing only the groups submitted to bleaching agents with hydrogen peroxide in different concentrations (G2 and G3), no significant difference was found (p > 0.05). Conclusions: After 10 days, the bleaching products tested in this study determined a significant increase in the average surface roughness values of the nanoparticulate composite resin.


Asunto(s)
Blanqueamiento de Dientes , Resinas Compuestas/análisis , Blanqueadores , Blanqueadores Dentales/uso terapéutico , Peróxido de Hidrógeno/uso terapéutico , Resinas Sintéticas , Técnicas In Vitro , Materiales Dentales
8.
Case Rep Dent ; 2014: 217172, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25371829

RESUMEN

Conservation of deciduous teeth with pulp alterations caused by caries and trauma is a major therapeutic challenge in pediatric dentistry as a result of the internal anatomy and life cycle characteristic. It is essential that the root canal procedures sanitizers have a performance in eliminating bacterial. In this context, antimicrobial photodynamic therapy (PAT) is promising and emerging as adjuvant therapy in an attempt to eliminate the microorganisms persistent to chemi-mechanical preparation. Since there is presence of oxygen in cells, photosensitizer activated by light can react with molecules in its vicinity by electrons' or hydrogen's transfer, leading to microorganism death. This paper reports the case of 4-year-old patient, female, with early childhood caries. The proposed endodontic treatment incuded chemomechanical treatment allied to PAT in the decontamination of root canals using methylene blue dye 50 µg/mL during 3-5 minutes and 40 J/cm(2) as energy density, taking into account the need for tissue penetration and effectiveness of PAT inside the dentinal tubules.

9.
Artículo en Inglés | MEDLINE | ID: mdl-24332330

RESUMEN

OBJECTIVE: To evaluate clinical oral and salivary parameters of individuals with juvenile idiopathic arthritis (JIA). STUDY DESIGN: Clinical parameters and whole saliva were collected from children aged 6 to 12 years with JIA (n = 36) and from a healthy, matched control group (n = 36). The clinical and salivary parameters evaluated were the dental caries (decayed, missing, or filled teeth), gingival and simplified oral hygiene indices, salivary flow rate, pH, buffer capacity, total protein, and secretory immunoglobulin A concentrations. RESULTS: JIA individuals presented poorer oral hygiene (P ≤ .05) but no difference in the dental caries and gingival indices. JIA patients presented an increase in total protein concentration (86%) and buffer capacity in the range of pH 6.9 to 6.0 (10%) and a reduction in initial pH (6%), buffer capacity in the range of pH ≥ 7.0 (50%), and immunoglobulin A concentration (27%) (P ≤ .05). CONCLUSIONS: JIA is associated with poor oral hygiene and salivary changes, including reductions in immune factors and an altered profile of salivary buffer capacity.


Asunto(s)
Artritis Juvenil/complicaciones , Caries Dental/etiología , Inmunoglobulina A Secretora/análisis , Saliva/metabolismo , Artritis Juvenil/inmunología , Artritis Juvenil/metabolismo , Estudios de Casos y Controles , Niño , Índice CPO , Caries Dental/diagnóstico , Femenino , Humanos , Masculino , Índice de Higiene Oral , Índice Periodontal , Saliva/química , Saliva/inmunología
10.
Rev. Odontol. Araçatuba (Impr.) ; 40(3): 9-13, set.-dez. 2019. ilus
Artículo en Portugués | LILACS, BBO - odontología (Brasil) | ID: biblio-1102184

RESUMEN

O traumatismo dentário acontece de maneira frequente, tendo maior prevalência em incisivos centrais, podendo ocorrer de várias formas, acometendo o tecido duro e o tecido de sustentação do dente. A severidade do trauma pode ocasionar um processo degenerativo ou uma lesão irreversível até a mortificação da polpa, por isso deve-se observar o estágio de desenvolvimento do dente para correta terapêutica diante de uma rizogênese completa ou incompleta. A necrose pulpar em um dente com ápice incompleto paralisa o seu processo de desenvolvimento o que torna necessário a realização da apicificação. O objetivo desse presente trabalho é relatar um caso clínico de traumatismo em um dente com rizogênese incompleta e a conduta terapêutica instituída para promover a apicificação do mesmo. Entretanto, Durante o tratamento foi um grande desafio promover o fechamento do ápice que fora paralisado após o trauma. Portanto, foi preciso realizar várias sessões de medicação intracanal com hidróxido de cálcio que é o material mais indicado para induzir a formação de barreira calcificada para fechamento completo do ápice(AU)


The dental trauma occurs frequently, having a higher prevalence in central incisors, and can occur in several ways, affecting the hard tissue and the tooth support tissue. The severity of the trauma can cause a degenerative process or an irreversible lesion until the mortification of the pulp, so the stage of development of the tooth must be observed for correct therapy in the presence of complete or incomplete rhizogenesis. Pulp necrosis in a tooth with an incomplete apex paralyzes its development process, which makes it necessary to perform the inoculation. The objective of this present work is to report a clinical case of trauma in a tooth with incomplete rhizogenesis and the therapeutic conduct instituted to promote the apicification of the same. However, during the treatment it was a great challenge to promote the closure of the apex that had been paralyzed after the trauma. Therefore, it was necessary to perform several intracanal medication sessions with calcium hydroxide, which is the most suitable material to induce the formation of a calcified barrier for complete closure of the apex(AU)


Asunto(s)
Humanos , Masculino , Adolescente , Traumatismos de los Dientes , Necrosis de la Pulpa Dental , Ápice del Diente/cirugía
11.
Arch Oral Biol ; 57(6): 630-5, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22196077

RESUMEN

OBJECTIVE: The aim of the study was to evaluate the effect of topical fluoride gel NaF 2% application on antioxidant parameters of whole saliva from children. DESIGN: The saliva mechanically stimulated with parafilm was collected from 25 children (6-12 years) attending the Clinic of Paediatric Dentistry of Universidade Cruzeiro do Sul, São Paulo, Brazil, before (control group) and immediately after application of neutral fluoride gel NaF 2% (fluoride-gel group), according to the Standards for Research Using Human Subjects, Resolution 196/96 of the USA National Health Council of 10/10/1996. Afterwards, pre-post ferric-reducing antioxidant power (FRAP), trolox-equivalent antioxidant capacity (TEAC), uric acid, reduced/oxidised glutathione content (GSH/GSSG) and total peroxidase activity (TPO) were evaluated in whole saliva of both groups. RESULTS: All non-enzymatic antioxidant parameters were augmented by fluoride-gel NaF 2% application, whereas a notable reduction (31%) of peroxidase activity was concomitantly observed in the children's saliva (p ≤ 0.05). Nevertheless, the reducing power of saliva was kept unaltered under these circumstances (p ≤ 0.05). CONCLUSIONS: Despite the reduced activity of peroxidase (an important antimicrobial and antioxidant enzyme), the topical fluoride gel NaF 2% favourably stimulated the release of non-enzymatic antioxidant components of saliva, sustaining the reducing power of saliva and the natural defences of the oral cavity.


Asunto(s)
Antioxidantes/farmacología , Fluoruros Tópicos/farmacología , Saliva/química , Fluoruro de Sodio/farmacología , Niño , Femenino , Glutatión/análisis , Humanos , Masculino , Oxidación-Reducción , Peroxidasa/análisis , Saliva/enzimología , Fluoruro de Sodio/administración & dosificación , Ácido Úrico/análisis
12.
Arch Oral Biol ; 55(7): 479-85, 2010 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-20510163

RESUMEN

OBJECTIVE: To evaluate the effect of astaxanthin on antioxidant parameters of salivary gland from diabetic rats. The hypothesis of the study was whether the supplementation of diabetic rats with astaxanthin might antagonize, or at least prevent, the defect in their antioxidative status. DESIGN: Wistar rats (n=32) were divided in 4 groups: untreated control, treated control, untreated diabetic and treated diabetic rats. Astaxanthin (20mg/kg body weight) was administered daily by gavage for 30 days. On day 23, diabetes was induced by injection of alloxan (60 mg/kg body weight). After 7 days of diabetes induction, the rats were killed and submandibular and parotid removed. Superoxide dismutase (SOD), catalase, glutathione peroxidase and reductase activities and the content of thiol groups were determined. Data were compared by ANOVA and the Tukey test (p<0.05). RESULTS: Diabetes caused a reduction of SOD, and thiol content and increase of catalase and glutathione peroxidase activities of submandibular gland whilst in the parotid gland diabetes caused an increase of thiol content and no effect in the antioxidant system. The astaxanthin restores the enzymatic activities in the salivary gland, however does not prevent its oxidative damage. CONCLUSION: The submandibular gland presented more susceptibility to oxidative alterations induced by diabetes. Astaxanthin presented a positive effect on the oxidative protection of the salivary gland from diabetic rats.


Asunto(s)
Antioxidantes/uso terapéutico , Diabetes Mellitus Experimental/prevención & control , Glándula Parótida/efectos de los fármacos , Glándula Submandibular/efectos de los fármacos , Aloxano , Animales , Antioxidantes/análisis , Catalasa/análisis , Catalasa/efectos de los fármacos , Diabetes Mellitus Experimental/enzimología , Depuradores de Radicales Libres/análisis , Glutatión Peroxidasa/análisis , Glutatión Peroxidasa/efectos de los fármacos , Glutatión Reductasa/análisis , Glutatión Reductasa/efectos de los fármacos , Masculino , Glándula Parótida/enzimología , Ratas , Ratas Wistar , Proteínas y Péptidos Salivales/análisis , Proteínas y Péptidos Salivales/efectos de los fármacos , Glándula Submandibular/enzimología , Compuestos de Sulfhidrilo/análisis , Superóxido Dismutasa/análisis , Superóxido Dismutasa/efectos de los fármacos , Xantófilas/uso terapéutico
13.
RFO UPF ; 20(2): 155-158, maio-agosto 2015. ilus
Artículo en Portugués | LILACS-Express | LILACS | ID: lil-771312

RESUMEN

Objetivo: o presente estudo quantificou o biofilme dental por meio do índice de higiene oral simplificado (IHO-S) de Greene e Vermillion, em crianças com erosão dentária, comparando-as com crianças sem esse problema de saúde bucal. Sujeitos e método: participaram do estudo quarenta crianças, de quatro a nove anos de idade, examinadas por um único examinador treinado de acordo com o índice de O´Brien para o diagnóstico de erosão dentária, sendo que vinte crianças apresentavam erosão nos dentes e vinte crianças não a apresentavam. Posteriormente, elas receberam orientaçõese padronização para a avaliação do IHO-S. Os dados foram analisados pela regressão logística univariada de Poisson. Valores de razão de prevalência (RP) e intervalos de confiança de 95% (IC 95%) foram calculados. Resultados: a média (desvio padrão) do IHO-S de crianças com erosão foi de 1,20 (0,40), menor que as crianças do grupo controle 1,75 (0,45). A quantidade de biofilme foi associada à presença de erosão dentária (RP IC 95% = 0.390; 0.180-0.833) e valor de p = 0.027. Conclusões: crianças com erosão nos dentes apresentaram uma quantidade significativamente menor de biofilme em relação às crianças sem esse problema. O biofilme pode ser um possível fator protetor contra os ataques ácidos e o desenvolvimento da erosão dentária.

14.
Pesqui. bras. odontopediatria clín. integr ; 15(1): 95-101, 2015. tab, graf
Artículo en Inglés | LILACS | ID: lil-796354

RESUMEN

To quantify dental biofilmthrough the simplified oral hygiene index (OHI-S) proposed by Greene and Vermillion in children with dental erosion comparing them to children without this oral health problem.Material and Methods:The study included 48 children aged 4-9 years treated at the Clinic of Pediatric Dentistry of the Cruzeiro do Sul University, examined by a single trained and calibrated examiner according to O'Brien index for the diagnosis of dental erosion, in which 24 children had erosion lesions in teeth and 24 children did not have this oral health problem. Later, children received guidance and standardization for the OHI-S evaluation. Data were analyzed by the Poisson univariate logistic regression. Prevalence ratio (PR) values and 95% confidence intervals (CI 95%) were calculated. Results:The mean (standard deviation) of the OHI-S of children who had dental erosion was 1.19 (0.38), lower than children in the group without dental erosion 1.73 (0.44). The amount of biofilm on teeth was associated with the presence of dental erosion (OR 95% CI = 0.393; 0188-0822) and p-value = 0.013. Regarding the reduction in the WSL dimension, no significant difference between groups was observed (p = 0.931). Conclusion:Children with dental erosion showed a significantly lower amount of biofilm compared to children without this oral health problem, and dental biofilm can be a possible protective factor against acid attacks and development of dental erosion...


Asunto(s)
Humanos , Masculino , Femenino , Preescolar , Niño , Erosión de los Dientes/diagnóstico , Placa Dental , Salud Bucal/educación , Índice de Higiene Oral , Distribución de Poisson , Estudios Transversales/métodos
15.
Biol Trace Elem Res ; 127(2): 154-63, 2009 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-18810331

RESUMEN

Several studies have shown the antidiabetic properties of sodium tungstate. In this study, we evaluated some biochemical parameters of the parotid salivary gland of streptozotocin-induced diabetic rats treated with sodium tungstate solution (2 mg/ml). The studied groups were: untreated control (UC), treated control (TC), untreated diabetic (UD), and treated diabetic (TD). After 2 and 6 weeks of treatment, parotid gland was removed and total protein and sialic acid (free and total) concentration and amylase and peroxidase activities were determined. Data were compared by variance analysis and Tukey test (p < 0.05). The sodium tungstate treatment modestly decreased the glycemia of streptozotocin-induced diabetic rats. At week 2 of the study, parotid gland of diabetic rats presented a reduction of total protein concentration (55%) and an increase of amylase (120%) and peroxidase (160%) activities, free (150%) and total (170%) sialic acid concentration. No alteration in the evaluated parameters at week 6 of the study was observed. Sodium tungstate presented no significant effect in parotid gland. Our results suggest that diabetes causes initial modification in biochemical composition of parotid. However, this gland showed a recovery capacity after 6 week of the experimental time. Sodium tungstate has no effect in peripheral tissues, such as salivary glands.


Asunto(s)
Diabetes Mellitus Experimental/metabolismo , Glándula Parótida/química , Glándula Parótida/efectos de los fármacos , Compuestos de Tungsteno/farmacología , Amilasas/metabolismo , Análisis de Varianza , Animales , Glucemia/análisis , Femenino , Ácido N-Acetilneuramínico/análisis , Glándula Parótida/enzimología , Peroxidasa/metabolismo , Distribución Aleatoria , Ratas , Ratas Wistar
16.
Braz. j. oral sci ; 13(1): 58-63, Jan-Mar/2014. tab
Artículo en Inglés | LILACS | ID: lil-709503

RESUMEN

Salivary glands contribute to oral health. It is therefore of interest to study therapies that may favor their function and protection. AIM: To evaluate the effect of astaxanthin, fish oil and association of them on enzymatic antioxidant system and functional parameters of salivary glands. METHODS: Healthy rats (n=32) were divided into 4 groups: untreated-control, astaxanthin-treated (1 mg/kg body weight- BW), fish oil-treated (10 mg EPA/kg BW and 7 mg DHA/kg BW), and fish oil plus astaxanthin-treated. A prophylactic dose was administered in each group daily by gavage, for 45 days. Superoxide dismutase (SOD), catalase, glutathione peroxidase, reductase, and α-amylase activities were determined in salivary glands and compared by ANOVA and Tukey post-test (p<0.05). RESULTS: Parotid gland presented increased catalase and glutathione system and unaffected SOD activity after astaxanthin and astaxanthin plus fish oil treatment (p<0.05). Fish oil stimulated only glutathione peroxidase activity of parotid gland (p<0.05). Submandibular gland presented stimulated SOD and catalase, and reduced glutathione reductase activities after fish oil and fish oil plus astaxanthin treatment (p<0.05). SOD and glutathione reductase activities were reduced by astaxanthin treatment in submandibular gland (p<0.05). Parotid gland presented increased α-amylase activity in all groups supplemented and submandibular glands presented no changes (p<0.05). CONCLUSIONS: Astaxanthin, fish oil and combination of them stimulated the antioxidant system and functional parameter of salivary glands, which could be beneficial to oral health.


Asunto(s)
Animales , Ratas , alfa-Amilasas , Antioxidantes/uso terapéutico , Carotenoides/uso terapéutico , Aceites de Pescado , Glándulas Salivales
17.
J Endod ; 34(10): 1211-4, 2008 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-18793922

RESUMEN

Diabetes can interfere in tissue nutrition and can impair dental pulp metabolism. This disease causes oxidative stress in cells and tissues. However, little is known about the antioxidant system in the dental pulp of diabetics. Thus, it would be of importance to study this system in this tissue in order to verify possible alterations indicative of oxidative stress. The aim of this study was to evaluate some parameters of antioxidant system of the dental pulp of healthy (n = 8) and diabetic rats (n = 8). Diabetes was induced by streptozotocin in rats. Six weeks after diabetes induction, a pool of the dental pulp of the 4 incisors of each rat (healthy and diabetic) was used for the determination of total protein and sialic acid concentrations and catalase and peroxidase activities. Data were compared by a Student t test (p

Asunto(s)
Antioxidantes/metabolismo , Pulpa Dental/metabolismo , Diabetes Mellitus Experimental/metabolismo , Animales , Antioxidantes/análisis , Glucemia/análisis , Peso Corporal , Catalasa/análisis , Pulpa Dental/enzimología , Diabetes Mellitus Experimental/enzimología , Femenino , Ácido N-Acetilneuramínico/análisis , Estrés Oxidativo/fisiología , Peroxidasas/análisis , Proteínas/análisis , Ratas , Ratas Wistar , Espectrofotometría , Estreptozocina
18.
Artículo en Portugués | LILACS | ID: lil-724202

RESUMEN

Objetivo: avaliar o índice de cárie dentária e parâmetros salivares comparando meninos e meninas.Métodos: a saliva total estimulada mecanicamente com parafilm foi coletada de 46 crianças saudáveis, com quatro a seis anos de idade, sendo 24 meninos e 22 meninas. As crianças foram divididas em subgrupos de acordo com o gênero (meninos e meninas) e presença de cárie dentária (cavidades de cárie, CC; livres de cárie, CF). A cárie dentária foi avaliada usando os critérios da OMS e Kappa=0,87. O grupo CC foi definido pela presença de no mínimo três superfícies com necessidade de restauração e o grupo CF pela ausência de dentes com lesões de cárie clinicamente detectáveis (ceo-s=o). Os parâmetros salivares avaliados foram o fluxo salivar, a concentração de proteína total e a atividade enzimática da peroxidase. Os resultados foram comparados por teste t de Student e Análise de Variância e teste de Tukey para contraste de média (p≤0,05). Resultados: crianças com cárie dentária apresentaram redução de 33% do fluxo salivar comparadas com crianças sem cárie dentária (p≤0,05). Entre as crianças com cárie dentária, as meninas apresentaram maior índice de cárie dentária comparadas com os meninos (98%, p≤0.05). As meninas apresentaram diferenças salivares mais pronunciadas comparados aos meninos, com maior concentração de proteína total e menor fluxo salivar e atividade da peroxidase (p≤0,05). Conclusão: o estudo sugere que o gênero pode influenciar o desenvolvimento de cárie dentária e parâmetros salivares de crianças...


Objective: To evaluate the dental caries index and salivary parametersin boys and girls. Method: Whole stimulated saliva by chewing Parafilm® was collected from 46 healthy children (24 boys and 22 girls) aged 4 to 6 years. The children were assigned to subgroups according to gender (boys andgirls) and dental caries (CC û caries cavities; CF û caries free). Dentalcaries was evaluated using the WHO criteria and kappa=0.87. The CC group was defined by the presence of at least three surfaces requiring restoration while the CF group was characterized by the absence of clinically detectable caries (ceo-s=0). The following salivary parameters were evaluated: salivary flow, total protein concentration andperoxidase enzymatic activity. The results were compared by theStudentÆs t-test, ANOVA and TukeyÆs multiple-comparison test (p≤0.05).Results: Children with dental caries presented a 33% reduction of salivary flow compared to the caries-free children (p≤0.05). Among the children with dental caries, the girls had a greater caries index than the boys (98%, p≤0.05). The girls presented more accentuated salivary parameters than boys, with greater total protein concentration, lower salivary flow and lower peroxidase activity (p≤0.05).Conclusion: This study suggests that gender might influence the development of dental caries and the salivary parameters in children...


Asunto(s)
Humanos , Masculino , Femenino , Niño , Caries Dental/prevención & control , Peroxidasa , Saliva/metabolismo , Análisis de Varianza , Estadísticas no Paramétricas , Glándulas Salivales Menores , Proteínas y Péptidos Salivales
19.
Rev. Assoc. Paul. Cir. Dent ; 70(2): 187-190, abr.-jun. 2016. ilus
Artículo en Portugués | LILACS, BBO - odontología (Brasil) | ID: lil-797071

RESUMEN

Objetivo: Apresentar caso clínico de tratamento estético das lesões de mancha branca após tratamento ortodôntico nos dentes superiores anteriores através de duas técnicas minimamente invasivas usando o sistema de infiltração de resina e microabrasão. Relato de caso: Paciente com 18 anos de idade apresentava lesões de manchas brancas inativas nos dentes 11, 12, 13, 21, 22, 23. O sistema de infiltrante de resina Icon (DMG, Hamburgo, Alemanha) foi utilizado nos dentes 11, 12, 13, enquanto os dentes 21, 22, 23 foram submetidos à microabrasão com Whiteness RM (FGM, Joinville, Santa Catarina, Brasil).Ambos os protocolos foram utilizados de acordo com as recomendações do fabricante.Nos dentes 21 e 22, o produto para microabrasão foi aplicado com espátula que acompanha o kit, enquanto que no dente 23 utilizou-se o mesmo produto aplicado com taça de borracha em baixa rotação, todos os procedimentos executados por um único operador. A microabrasão com taça de borracha proporcionou uma superfície mais lisa e homogênea.Ambos os produtos tiveram resultados satisfatórios na resolução estética das lesões de mancha branca após um ano de acompanhamento. Conclusão: Os dois produtos apresentam bom desempenho e resolutividade para os problemas estéticos de lesões de manchas brancas inativas, entretanto deve-se levar em conta o tempo clínico, toxicidade, a atividade da lesão e a possibilidade de desgaste da estrutura dentária.


Objective: To present a case report of aesthetic treatment of post orthodontic white--spot lesions in the anterior superior teeth through two minimally invasive technique susing resin infiltration system or microabrasion. Case report: Patient 17 years of age had inactive white-spots lesions on teeth 11, 12, 13, 21, 22, 23. The teeth 11, 12, 13 received the resin infiltrating system Icon (DMG, Hamburg, Germany) while the teeth 21, 22, 23were submitted to microabrasion with Whiteness RM (FGM, Joinville, Santa Catarina, Brazil). Both protocols were used according to manufacturer’s recommendations. In the teeth 21 and 22, the product of the microabrasion was applied with a spatula that accompanied the kit, while the tooth 23 received the same product applied with a rubbercup at low speed turbine, all by a single operator. The microabrasion with rubber cupoccasioned a more smooth and homogeneous surface. Both products had satisfactory results in aesthetic resolution of the white-spots lesions, for a 1-year follow-up. Conclusion:Although both products are able to resolve aesthetic problems of inactive white-spots lesions, it should be taken into account the clinical time, toxicity, the activity ofthe lesion and the possibility of wear of the tooth structure.


Asunto(s)
Humanos , Masculino , Femenino , Adulto Joven , Caries Dental/clasificación , Caries Dental/complicaciones , Caries Dental/diagnóstico , Esmalte Dental/crecimiento & desarrollo , Esmalte Dental/lesiones , Esmalte Dental/química , Estética Dental/estadística & datos numéricos , Microabrasión del Esmalte/efectos adversos , Microabrasión del Esmalte , Ortodoncia/organización & administración
20.
Full dent. sci ; 7(25): 92-96, jan.2016. tab
Artículo en Portugués | LILACS | ID: lil-790083

RESUMEN

Alguns critérios são utilizados para qualificar restaurações com cimento de ionômero de vidro (CIV), como a rugosidade superficial. O aumento desta resulta na retenção de substratos, pigmentos e micro-organismos, aumentando o risco de recidivas de cárie e redução da longevidade da restauração. Assim, este estudo teve como objetivo avaliar, in vitro, a rugosidade superficial de diferentes CIVs, analisando a influência do polimento e da proteção superficial. Três CIVs foram analisados: dois convencionais (Vitro Fil R - DFL; Ketac Molar Easymix - 3M) e um modificado por resina (Vitro Fil LC - DFL). Foram confeccionados 60 corpos de prova, divididos em seis grupos (n=10), 3 controles (G1, G3 e G5) que não receberam qualquer tratamento de superfície, e 3 experimentais (G2, G4 e G6), cujos espécimes foram desgastados com ponta diamantada e polidos com discos abrasivos, com posterior aplicação de protetor superficial. Para análise da rugosidade superficial, utilizou-se o rugosímetro Surftest 301. Os valores obtidos foram submetidos à análise de variância (ANOVA) e teste de Tukey, nível de significância de 5%. O CIV modificado por resina apresentou menor valor de rugosidade superficial, embora todos os CIVs testados tenham apresentado aumento significativo da rugosidade após o tratamento de superfície. Em relação à proteção superficial, o grupo tratado com glaze apresentou rugosidade inferior aos grupos tratados com vaselina sólida e esmalte cosmético. O CIV modificado por resina apresentou rugosidade superficial inferior aos CIVs convencionais, e dentre os métodos de proteção, o glaze determinou o menor valor de rugosidade superficial média...


Some criteria such as surface roughness are used to qualify the restorations with glass ionomer cement (GIC). The increased roughness results in the retention of substrate, pigments and microorganisms, increasing the risk of developing caries and reducing the longevity of the restoration. The aim of this study was to evaluate, in vitro, the surface roughness of GICs used as a restorative material, analyzing the influence of polishing and surface protection. Three GICs were analyzed: two conventional (Vitro Fil R û DFL; Ketac Molar Easymix - 3M) and one resin-modified (Vitro Fil LC - DFL). Sixty specimens were prepared, divided into six groups (n=10), 3 controls (G1, G3 and G5) that received no surface treatment, and 3 experimental groups (G2, G4 and G6), in which the specimens were subjected to wear with diamond bur and polishing with abrasive discs, with subsequent application of protective surface. For analysis of surface roughness, we used the rugosimeter Surftest 301. The values obtained were statistically analyzed by ANOVA and TukeyÆs test with a significance level of 5%. The resin modified GIC showed the lowest surface roughness. Although all GICs tested showed significant increase in roughness after surface treatment. The group treated with the glaze had less roughness than groups treated with solid vaseline and cosmetic enamel. The resin modified GIC had less surface roughness than conventional GICs, and among studied methods the glaze has presented lower values for average surface roughness...


Asunto(s)
Humanos , Cementos de Ionómero Vítreo/química , Restauración Dental Permanente , Propiedades de Superficie , Análisis de Varianza , Técnicas In Vitro/métodos
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