RESUMEN
Four new diterpenoides, isolophanthins A-D (1-4) together with seven known abietane diterpenoides (5-11), have been isolated from Isodon lophanthoides var. gerardianus. The new diterpenoides were elucidated by spectroscopic analysis. Some of them showed significant activities against HBsAg and HBeAg of hepatitis B virus in Hep G 2.2.15 cells, as well as the human tumor cell lines, HL-60, A-549, MOLT-4, and BEL-7402.
Asunto(s)
Antineoplásicos Fitogénicos/química , Antivirales/química , Diterpenos/química , Virus de la Hepatitis B/efectos de los fármacos , Isodon/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/toxicidad , Antivirales/aislamiento & purificación , Antivirales/toxicidad , Línea Celular Tumoral , Diterpenos/aislamiento & purificación , Diterpenos/toxicidad , Ensayos de Selección de Medicamentos Antitumorales , Antígenos de Superficie de la Hepatitis B/efectos de los fármacos , Antígenos e de la Hepatitis B/efectos de los fármacos , Humanos , Espectroscopía de Resonancia Magnética , Componentes Aéreos de las Plantas/químicaRESUMEN
OBJECTIVE: To reveal the effects of ecological factors on geo-herbalism,by researching the correlation between yields or qualities of Curcuma wenyujin and ecological factors. METHODS: All measured dates were divided into three components, meteorological factors, soil conditions, the yields and qualities of geo-herbals, and analyzed through canonical correlation using SPSS software. RESULTS: The results showed that there were two canonical correlation models to explain correlation between meteorological factors and yields or qualities of medicinal materials, as well as soil conditions and yields or qualities, and both of canonical correlations reached the significant or extremely significant level. The analysis indicated that main ecological factors affecting the yields or qualities of medicinal materials were average temperature from May to June,the days of high temperature (over 35 degrees C) and average rainfall from August to October, annual average rainfall, altitude, available potassium content in soil. CONCLUSIONS: This study has tentatively made clear which ecological factors to affect the yields and qualities of Curcuma wenyujin; and provided some basis for revealing geo-herbalism of Curcuma wenyujin.
Asunto(s)
Clima , Curcuma/crecimiento & desarrollo , Ecosistema , Plantas Medicinales/crecimiento & desarrollo , Suelo , Curcuma/química , Fertilizantes , Modelos Estadísticos , Tallos de la Planta/química , Tallos de la Planta/crecimiento & desarrollo , Plantas Medicinales/química , Control de Calidad , Estaciones del Año , Suelo/análisis , TemperaturaRESUMEN
OBJECTIVE: The DNA fingerprints of Curcuma wenyujin from various habitats were generated by using SRAP markers to find the feasibility in analyzing their relationship. METHODS: The DNA polymorphism of Curcuma wenyujin from various habitats were detected by SRAP molecular markers. RESULTS: We had screened five pairs of primer combinations, and built the DNA fingerprints of Curcuma wenyujin from various habitats. The phylogenetic clustering results revealed that the genetic difference in Curcuma wenyujin from various habitats were little. CONCLUSION: SRAP markers may offer some evidence for protecting and exploiting of Curcuma wenyuji.