RESUMEN
We studied toxicity of recombinant Streptococcus pneumoniae pneumolysin protein in experiments on mice and its cytopathogenic effect on cultures of Vero green monkey kidney cells and human lung carcinoma A549 cells in vitro. In vivo and in vitro experiments proved the absence of compromised toxicity and direct cytopathogenic action of the recombinant protein.
Asunto(s)
Proteínas Bacterianas , Proteínas Recombinantes , Streptococcus pneumoniae , Estreptolisinas , Estreptolisinas/toxicidad , Estreptolisinas/genética , Animales , Proteínas Bacterianas/toxicidad , Proteínas Bacterianas/genética , Chlorocebus aethiops , Ratones , Células Vero , Streptococcus pneumoniae/efectos de los fármacos , Humanos , Proteínas Recombinantes/toxicidad , Proteínas Recombinantes/genética , Células A549RESUMEN
The species identity of the studied lactobacillus strains was confirmed by matrix-activated laser desorption/ionization with time-of-flight ion separation (MALDI-TOF mass spectrometry). Lactobacillus strains differed in the dynamics of lactic acid accumulation and changes in the pH of the culture medium. The culture medium affected adhesion ability of lactobacilli. The ability to adhere does not affect the formation of biofilms by lactobacillus strains except for the L. acidophilus La5 strain, which has low adhesion ability and fewer microbial cells detected after mechanical destruction of the biofilm. The metabiotics of the lactobacillus culture medium have an antagonistic effect on conditionally pathogenic microorganisms. Adhesion, biofilm formation, and antagonistic activity of probiotic lactobacillus strains are strain-specific properties.
Asunto(s)
Adhesión Bacteriana , Biopelículas , Lactobacillus , Probióticos , Biopelículas/crecimiento & desarrollo , Biopelículas/efectos de los fármacos , Probióticos/farmacología , Adhesión Bacteriana/efectos de los fármacos , Lactobacillus/fisiología , Concentración de Iones de Hidrógeno , Medios de Cultivo/química , Ácido Láctico/metabolismo , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Antibiosis/fisiología , Lactobacillus acidophilus/fisiologíaRESUMEN
Pneumolysin (Ply) is a target for the development of serotype-independent pneumococcal vaccines, an important condition for the efficacy of which is their ability to activate innate immunity with the subsequent formation of adaptive immunity. In this study, the ability of recombinant full-length Ply (rPly) of pneumococci to induce TLR expression and maturation of dendritic cells generated from mouse bone marrow was evaluated. It was shown that rPly in vitro increased the number of dendritic cells expressing Toll-like receptor 4 (TLR4) on the membrane. rPly caused maturation of dendritic cells generated from mouse bone marrow, which manifested in a decrease in the number of progenitor cells (CD34), an increase in the number of cells expressing the adhesion molecule CD38, costimulatory molecules CD80 and CD86, molecules of terminal differentiation of dendritic cells CD83, as well as molecules of antigenic presentation of the major histocompatibility complex class II.