Hydroxyapatite coating on an aluminum/bioplastic scaffold for bone tissue engineering.

Three-dimensional printing can produce scaffolds with shapes and dimensions tailored for practical clinical applications. Enhanced osteoconductivity of such scaffolds is generally desired. Hydroxyapatite (HA) is an inorganic ceramic that can be used to coat such scaffolds and to accelerate healing during the bone restoration process. In this study, HA-coated aluminum/bioplastic scaffolds were fabricated, and their structural characteristics and osteoconductivity were evaluated. Aluminum/bioplastic scaffolds were fabricated by three-dimensional printing, and HA slurries with solids loadings of 10-20 vol% were used for coating. As solids loadings increased, the thickness of the coating layers slightly increased, whereas pore sizes decreased. The average compressive strength was comparable to that of cancellous bone. Potential osteoconductivity was tested by simulated body fluid immersion for 28 days, and the formation of the HA phase on the surface along with a weight increase indicates the potential bioactivity of the samples.

Structure and properties of in situ reactive blend of polylactide and thermoplastic starch.

In this study, in situ reactive extrusion of polylactide and thermoplastic starch modified with chloropropyl trimethoxysilane coupling agent (PLA/mTPS) is proposed. The success of covalent bond formation between PLA matrix and mTPS phase is clarified by two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy with 1H1H TOCSY mode. This chemically bound PLA with starch gives the remarkable compatibility in the PLA/mTPS film, with not only a decreased glass transition temperature (47 °C) but also an increased crystallinity of PLA (Χc of 50%). It consequently increases oxygen barrier significantly and also enhances the film flexibility as observed from the drastic increase of elongation at break (from 3% to 50%). Moreover, the PLA/mTPS 60/40 (w/w) film exhibits the accelerated degradation as compared with pure PLA film.

Titanium dioxide and fluoropolymer-based coating for smart fabrics with antimicrobial and water-repellent properties.

In the coronavirus disease 2019 pandemic, protective clothing is required for medical staff at risk of infection. This study proposes functional smart fabrics with antimicrobial and water-repellent properties, using titanium dioxide (TiO2) and fluoropolymer-based precursors as coating materials. Experimental results indicated a uniform distribution of TiO2 particles with an average size below 200 nm throughout the fabric. A zone of inhibition test revealed that the fabric inhibited bacterial growth, specifically of Staphylococcus aureus and Klebsiella pneumoniae, before and after 10 wash cycles of the fabric. In wetting angle measurements, the contact angles of water droplets on the fabric ranged from 120° to 139°. A water repellency test confirmed that the coated fabrics retained their water-repellent property after 10 wash cycles.

Plausible molecular and crystal structures of chitosan/HI type II salt.

Chitosan/HI type II salt prepared from crab tendon was investigated by X-ray fiber diffraction. Two polymer chains and 16 iodide ions (I(-)) crystallized in a tetragonal unit cell with lattice parameters of a = b = 10.68(3), c (fiber axis) = 40.77(13) A, and a space group P4(1). Chitosan forms a fourfold helix with a 40.77 A fiber period having a disaccharide as the helical asymmetric unit. One of the O-3... O-5 intramolecular hydrogen bonds at the glycosidic linkage is weakened by interacting with iodide ions, which seems to cause the polymer to take the 4/1-helical symmetry rather than the extended 2/1-helix. The plausible orientations of two O-6 atoms in the helical asymmetric unit were found to be gt and gg. Two chains are running through at the corner and the center of the unit cell along the c-axis. They are linked by hydrogen bonds between N-21 and O-61 atoms. Two out of four independent iodide ions are packed between the corner chains while the other two are packed between the corner and center chains when viewing through the ab-plane. The crystal structure of the salt is stabilized by hydrogen bonds between these iodide ions and N-21, N-22, O-32, O-61, O-62 of the polymer chains.

Molecular and crystal structures of chitosan/HI type I salt determined by X-ray fiber diffraction.

The three-dimensional structure of chitosan/HI type I salt was determined by the X-ray fiber diffraction technique and linked-atom least-squares refinement method. Two polymer chains and four iodide ions (I(-)) crystallized in a monoclinic unit cell with dimensions a = 9.46(2), b = 9.79(2)], c (fiber axis)=10.33(2)A, beta = 105.2(2) degrees and a space group P2(1). Chitosan chains adopted an extended twofold helical conformation that was stabilized by O-3...O-5 hydrogen bonds, and the O-6 atom adopted nearly gt orientation. Polymer chains zigzag along the b-axis and directly connect to each other by N-2...O-6 hydrogen bonds. Two columns of iodide ions were shown to pack at the bending points of the zigzag sheets, and their locations are closely related to those of water columns in the hydrated chitosan. The iodide ions stabilized the salt structure by forming hydrogen bonds with the N-2 and O-6 atoms of the polymer chains together with an electrostatic interaction between N-2 and the iodide ions.

Two different molecular conformations found in chitosan type II salts.

The type II structure of chitosan acidic salts prepared from crab tendon in solid state was studied using an X-ray fiber diffraction technique together with the linked-atom least-squares (LALS) technique. The cylindrical Patterson method was applied to confirm the molecular conformation of the chitosan. It was shown that there are two different helical conformations for type II salts. One is the relaxed twofold helix having a tetrasaccharide as an asymmetric unit as found in chitosan.HCl salt, which was previously reported as a conformation of chitosan.HCOOH salt. The other is the fourfold helix having a disaccharide as an asymmetric unit newly found in chitosan.HI salt.