IVF protocol efficacy in women with expected suboptimal response depending on ovary stimulation mode.

INVESTIGATION OBJECTIVE: IVF protocol efficacy estimation in women with expected suboptimal response depending on ovary stimulation mode. MATERIALS AND TECHNIQUE: A randomized controlled study embracing results of 51 IVF cycle in women with ovary suboptimal response. The suboptimal response prognostic analysis was performed basing on ≤9 oocyte cumulus complexes obtained in previous IVF programs, the presence of no less than 5-9 antral follicles in both oocytes and amount of anti-Mullerian Hormone ≥0,8 ng/mL. In Group I (n = 25), the stimulation was performed by recombinant corifollitropin alfa combined with highly purified urinary gonadotropin, while in Group II (n = 26) it was made by means of recombinant follitropin/lutropin alfa within the protocol of applying gonadotropin-releasing hormone antagonists. RESULTS: The total gonadotropin dose in Group II patients was authentically lower compared to Group I (p˂,01). No statistical difference between the two studied groups was detected concerning the number of obtained oocytes, 2pn zygote, good-quality transferred embryos and clinical pregnancy rate (p>.05). Embryo cryopreservation was performed only for group-II patients. CONCLUSION: Corifollitropin alfa administration combined with highly purified menotropin in IVF cycles for suboptimal responders is quite effective, however, this strategy has no preference over other stimulation modes. The strategy of using recombinant follitropin/lutropin alfa can be promotive to IVF outcomes for suboptimal responders by means of embryo banking. ClinicalTrials.gov Identifier: NCT03177538.

[Gestagenic and contraceptive activity of new synthetic progesterone analogs in experimental animals].

The gestagenic activity of new 17alpha-hydroxyprogesterone analogs was studied in experiments on infantile female rabbits (Clauberg-McPhail assay) and ovariectomized animals (Comer-Allen assay). The new steroidal preparations produced significant secretory transformations in immature rabbit endometrium. The maximum gestagenic activity was observed for butagest, while the minimum effect was produced by duhydrogesterone (dufaston). Butagest, megestrol capronate, and AMOL isopropyl ester showed the ability to maintain pregnancy in ovariectomized female rabbits. The compositions of gestagens (0.8 mg/kg) with ethinylestradiol (0.04 mg/kg) produced high contraceptive effect in rats.

[Effects of verapamil and gynipral on the uterine contractile activity in rats]. / Vliianie verapamila i giniprala na sokratitel'nuiu deiatel'nost' matki krys.

Verapamil and gynipral administered to pregnant rats in doses on a clinical level suppress the uterine contractions, which is manifested by a decrease in the amplitude and frequency of the biopotential. The joint administration of gynipral and verapamil (in half doses) resulted in a pronounced tocolytic effect.

[The effect of a peptide preparation made from ovaries on reproductive function]. / Vliianie peptidnogo preparata iz iaichnikov na reproduktivnuiu funktsiiu.

A study was done on the action of the low molecular peptide preparation isolated from cow ovaries by the acetic acid extraction, on the rat ovary function. It was revealed that this preparation: 1) does not possess the gonadotrophic activity; 2) enhances the sensitivity of immature rat ovaries to the human chorionic gonadotrophin; 3) increases the number of ovulating oocytes in neonatal androgenized rats in gonadotrophic stimulation of the ovary function; 4) does not influence the character and duration of the estrous cycle in intact adult rats.

[The effect of thalicoside on reproductive function in an experiment]. / Vliianie talikozida na reproduktivnuiu funktsiiu v éksperimente.

Talicoside, a triterpene glycoside from Thalictrum minus L., given in an oral dose og 1 mg/kg for 5 days was demonstrated to cause ovulation stimulation induced by intravenously injecting 1.2% solution of copper acetate into the rabbits. The agent enterally administered in a dose of 1 mg/kg to rats for 5 days reduced the levels of luteinizing hormone and elevated serum follicle-stimulating hormone in proestrous and estrous.

[Therapeutic efficacy of novel 17alpha-hydroxyprogesterone derivatives in experimental preterm labor]. / Terapevticheskaia aktivnost' novykh proizvodnykh 17al'fa-oksiprogesterona pri nevynashivanii beremennosti v éksperimente.

The ability of a series of new 17 alpha-Hydroxyprogesteron derivatives (acetomepregnol, AMOL phenyl propyoate, butagest) in preventing the prostaglandin-induced preterm labor was experimentally studied in mice. It was found that all the synthetic gestagens studied, especially butagest, maintained pregnancy in all test animals on the model of enzaprost F induced abortion. The proposed progestogens can be used to prevent abortion in pregnancies in cases of risk from diseases connected with excess prostaglandin secretion.

[Experimental progestogenic activity of novel derivatives of 17alpha-hydroxyprogesterone]. / O gestagennoi aktinosti novykh proizvodnykh 17alpha-gidroksiprogesterona v éksperimente.

Experiments on infantile female rabbits showed that new 17a-hydroxyprogesterone derivatives (AMOL phenyl propionate, AMOL isopropyl ester) possess a pronounced gestagenic activity. In particular, AMOL isopropyl ester is capable of maintaining pregnancy in ovariectomized animals (Corner-Allen assay).

[Effect of instenon on the uterine contractile activity]. / Vliianie instenona na sokratitel'nuiu deiatel'nost' matki v éksperimente.

The effect of instenon upon the uterine contractile activity was studied in pregnant rats. The drug administration at a dose resulted in significant suppression of the myometrium contractility. The effect was not decreased when instenon was injected against the background of oxytocin. On the other hand, the concurrent injection of oxytocin upon a single or long-term administration of instenon produced a pronounced stimulating action on the myometrium contractility.