RESUMEN
Trichinellosis is a worldwide important food-borne zoonosis caused mainly by ingesting raw or undercooked pork infected with Trichinella spiralis larvae. The development of vaccine is needed for preventing swine from Trichinella infection to ensure pork safety. Previous studies showed that T. spiralis serine protease 1.2 (TsSP1.2) is a vaccine candidate against Trichinella infection. In this study, the complete TsSP1.2 cDNA sequences were cloned into pcDNA3.1, and the rTsSP1.2 DNA was transformed into attenuated Salmonella typhimurium strain ΔcyaSL1344. Oral vaccination of mice with Salmonella-delivered rTsSP1.2 DNA vaccine induced an obvious intestinal mucosal IgA response and a systemic Th1/Th2 immune response; the vaccinated mice showed a 33.45% reduction of intestinal adult worms and 71.84% reduction of muscle larvae after T. spiralis larval challenge. The protection might be due to the rTsSP1.2-induced production of specific anti-TsSP1.2 sIgA, IgG, IgG1/IgG2a, and secretion of IFN-γ, IL-4 and IL-10, which protected intestinal mucosa from the parasite invasion, inhibited worm development and reduced female fecundity. The results indicate that the attenuated Salmonella-delivered rTsSP1.2 DNA vaccine offers a prospective strategy for the prevention and control of animal Trichinella infection.
Asunto(s)
Proteínas del Helminto/inmunología , Inmunidad Mucosa , Serina Proteasas/inmunología , Trichinella spiralis/inmunología , Vacunas de ADN/administración & dosificación , Administración Oral , Animales , Femenino , Ratones , Ratones Endogámicos BALB C , Microorganismos Modificados Genéticamente/genética , Salmonella typhimurium/genética , Vacunación , Vacunas Atenuadas/administración & dosificaciónRESUMEN
The excretory-secretory (ES) antigens from Trichinella spiralis muscle larvae are the most commonly used diagnostic antigens for trichinellosis, but specific IgG antibodies were not detected in early stage of infection. The aim of this study was to identify early diagnostic antigens from ES proteins of intestinal infective larvae (IIL), the first invasive stage of T. spiralis. Six bands (92, 52, 45, 35, 32, and 29 kDa) of IIL ES proteins were recognized by infection sera in Western blotting as early as 10 days post infection. Total of 54 T. spiralis proteins in six bands were identified by shotgun LC-MS/MS, 30 proteins were annotated, and 27 had hydrolase activity. Several proteins (serine protease, putative trypsin, deoxyribonuclease II family protein, etc.) could be considered as the potential early diagnostic antigens for trichinellosis. Our study provides new insights for screening early diagnostic antigens from intestinal worms of T. spiralis.
Asunto(s)
Antígenos Helmínticos/inmunología , Proteínas del Helminto/inmunología , Proteómica , Trichinella spiralis/inmunología , Triquinelosis/diagnóstico , Animales , Anticuerpos Antihelmínticos/sangre , Western Blotting , Electroforesis en Gel de Poliacrilamida , Cromatografía de Gases y Espectrometría de Masas , Proteínas del Helminto/metabolismo , Larva/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Músculos/metabolismo , Músculos/parasitología , Serina Proteasas/inmunología , Organismos Libres de Patógenos Específicos , Porcinos , Triquinelosis/inmunologíaRESUMEN
In this work, we report the complete mitochondrial genome sequence of a German shepherd. The total length of the mitogenome was 16,727 bp. It contained the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a non-coding control region (D-loop region). The arrangement of these genes was the same as that found in other dogs. All the protein initial codons were ATG, except for ND2, ND3 and ND5 starting with ATA. Sequence comparison indicates that D-loop region harbors most sequence variations.
Asunto(s)
Perros/genética , Variación Genética , Genoma Mitocondrial/genética , Análisis de Secuencia de ADN , Animales , Genes de ARNr , Anotación de Secuencia Molecular , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , ARN de Transferencia/genéticaRESUMEN
In order to establish the foundation for modernization of Chinese herbal medicine, discovery of the functional genes related with the bioactive components of Chinese herbs and the rules of their expression was progressed for determining the regulatory mechanism, obtaining the core metabolic pathway and key regulatory factors of the medicinal components synthesis. In this article, the studies about plant's functional genes at home and abroad was introduced in three aspects: the pattern plants (rice, thaliana, etc.), the main technologic strategy (gene expression differentia, sequential tag of gene expression, DNA microarray technique and gene expression sequential analysis) and bio-informative method (comparative genomics). The current status of researches involving biosynthesis genes of flavonoids compounds and taxol, and the plant P450 genes were introduced. And the study of dendrobium functional genes conducted in authors' lab was also reviewed.