[Epidemiological survey of the association between non alcoholic fatty liver disease and metabolic syndrome in civil servants of Chongqing city].

OBJECTIVE: To investigate the association between non alcoholic fatty liver disease (NAFLD) and metabolic syndrome (MS). METHODS: A cross-sectional multiple-stage stratified survey was performed. A total of 2190 civil servants of Chongqing city were invited to participate in the survey covering physical examination, serum biochemistry-profile and ultrasonographic examination of liver. RESULTS: Of 2176 valid questionnaires, altogether 455 cases were diagnosed as NAFLD and 231 individuals were diagnosed as MS. The prevalence of obesity, hyperglycemia, blood lipid disturbance, primary hypertension, NAFLD and MS was 38.3%, 5.5%, 31.7%, 29.9%, 20.9% and 10.6% respectively, which was increased along with aging (chi2 = 31.775, P = 0.000; chi2 = 25.985, P = 0.000; chi2 = 44.818, P = 0.000; chi2 =149.802, P = 0.000; chi2 = 61.302, P = 0.000; chi2 = 43.508, P = 0.000 a partly). The prevalence of obesity, hyperglycemia, dyslipidemia, primary hypertension, metabolic syndrome was significantly higher than those in control group (chi2 = 384.554, P = 0.000; chi2 = 25.597, P = 0.000; chi2 = 370.849, P = 0.000; chi2 = 40.252, P = 0.000; chi2 = 215.077, P = 0.000 separately), and the level of body mass index (BMI), fasting plasma glucose (FPG), triglyceride (TG), systolic blood pressure (SBP), diastolic blood pressure (DBP) in NAFLD group was remarkably higher than those in control group (t = 26.308, P = 0.000; t = 6.055, P = 0.000; t = 15.980, P = 0.000; t = 10.550, P = 0.000; t = 13.628, P = 0.000 respectively), while the level of high-density lipoprotein cholesterol (HDL-C) was on the opposite (t = 20.067, P = 0.000). Compared with the control group, odds risk for NAFLD was 22.82 folds (95% CI: 12.64-41.19) in obesity, 20.97 folds (95% CI: 11.21-39.24) in hyperglycemia, 24.40 folds (95% CI: 13.51-44.07) in dyslipidemia, 15.73 folds (95% CI: 8.66-28.60) in primary hypertension, while the risk for NAFLD was the highest in MS (OR = 31.06, 95% CI: 17.12-56.35). There were simple or multiple metabolic disorders in 455 individuals diagnosed as NAFLD, and 21 case (4.6%) with obesity, hyperglycemia, dyslipidemia and primary hypertension. CONCLUSION: NAFLD is closely related with MS, which may be considered as a feature of MS.

[Study on the change of myocardial cell and matrix in the rats with insulin resistance and type 2 diabetes mellitus].

OBJECTIVE: To explore the injury of insulin resistance on cardiac muscle cell and matrix, and the relationship between insulin resistance and diabetic cardiomyopathy. METHODS: Twenty four Wistar rats of 6 months were randomly divided into normal control (N), insulin resistance group (I), diabetic group (D). Euglycemic insulin clamp technique (EICT) was used to determine insulin resistance (IR). Cadiocyte apoptosis was evaluated by TUNEL. Heart weight (HW) and body weight (BW) were measured to calculate HW/BW. Ultra-microstructure of cardiac muscle cell and structure of heart was observed. Masson dyeing, hydroxyproline detection and immunohistochemistry were used to measure the levels of collagen protein. RESULTS: Compared with controls, GIR decreased remarkably in D group and I group (P < 0.01). The number of apoptosis cell in I group was lower than that of D group (P < 0.01), and higher than that of N group (P < 0.01). Injury change of ultramicrostructure of myocardial cell was observed in the rats with type 2 diabetes mellitus or insulin resistance. Interstitial fibrosis of heart occurred in D group and I group. Content of Hydroxyproline, the level of I , III type of collagen, and the total level of collagen in I group were lower than those in D group, and higher than those in N group (P < 0.05). CONCLUSION: Insulin resistance in the rats with type 2 diabetes mellitus or insulin resistance can injury myocardial cell and matrix.