RESUMEN
Barley is one of the most important cereal crops in the world, and its value as a food is constantly being revealed, so the research into and the use of barley germplasm are very important for global food security. Although a large number of barley germplasm samples have been collected globally, their specific genetic compositions are not well understood, and in many cases their origins are even disputed. In this study, 183 barley germplasm samples from the Shanghai Agricultural Gene Bank were genotyped using genotyping-by-sequencing (GBS) technology, SNPs were identified and their genetic parameters were estimated, principal component analysis (PCA) was preformed, and the phylogenetic tree and population structure of the samples were also analyzed. In addition, a genome-wide association study (GWAS) was carried out for the hulled/naked grain trait, and a KASP marker was developed using an associated SNP. The results showed that a total of 181,906 SNPs were identified, and these barley germplasm samples could be roughly divided into three categories according to the phylogenetic analysis, which was generally consistent with the classification of the traits of row type and hulled/naked grain. Population structure analysis showed that the whole barley population could be divided into four sub-populations (SPs), the main difference from previous classifications being that the two-rowed and the hulled genotypes were sub-divided into two SPs. The GWAS analysis of the hulled/naked trait showed that many associated loci were unrelated to the Nud/nud locus, indicating that there might be new loci controlling the trait. A KASP marker was developed for one exon-type SNP on chromosome 7. Genotyping based on the KASP assay was consistent with that based on SNPs, indicating that the gene of this locus might be associated with the hulled/naked trait. The above work not only lays a good foundation for the future utilization of this barley germplasm population but it provides new loci and candidate genes for the hulled/naked trait.
Asunto(s)
Estudio de Asociación del Genoma Completo , Hordeum , Filogenia , Polimorfismo de Nucleótido Simple , Hordeum/genética , Estudio de Asociación del Genoma Completo/métodos , China , Sitios de Carácter Cuantitativo , Genotipo , Banco de Semillas , Genoma de Planta , Variación Genética , Análisis de Componente Principal , FenotipoRESUMEN
A full understanding of the material properties of skin tissue is crucial for exploring its tribo-mechanical behaviour. It has been widely accepted that the mechanical behaviour of skin tissue for both small and large deformations can be accurately described using a hyperelastic model, such as the one developed by Ogden. However, obtaining these Ogden parameters for in-vivo skin by in-vivo experiments no matter the indentation or suction tests is a significant challenge. The mathematical model used to describe the material behaviour during the test should consider not only the material nonlinearity but also the geometrical conï¬nement of the tissue, the large deformations induced, and the fact that the specimens are relatively thin. A range of contact models is available to describe the contact behaviour during the indentation test. However, none of them can be used for hyperelastic materials with small thickness under large deformations. Simultaneously explaining material nonlinearity and geometric nonlinearity, either through theoretical equations or numerical calculations, poses a significant challenge. In this research, we propose a pragmatic method to obtain Ogden parameters for in-vivo skin tissue by combining experimental indentation results and numerical simulations. The indentation tests were used to obtain the force-indentation depth curves, while the numerical simulations were used to obtain the strain fields. The method assumes the material behaviour of specimens can be linearized in each small deformation increment, and the contact model developed by Hayes can be applied to accommodate each increment. Then, the linear elastic behaviour in each increment can be described by the elastic modulus E which were obtained using Hayes model, and the principal stresses in each increment were subsequently obtained using Hooke's law. By combining all stress fields, overall stress-strain curves can be constructed, from which the hyperelastic Ogden parameters can be obtained. A second numerical simulation of the hyperelastic indentation was then performed using the obtained Ogden parameters, allowing a comparison of the experimental and simulated relationships between force and indentation.
Asunto(s)
Elasticidad , Ensayo de Materiales , Ensayo de Materiales/instrumentación , Fenómenos Biomecánicos , Piel , Pruebas Mecánicas , Estrés MecánicoRESUMEN
Salt stress is a major abiotic stress that threatens global rice production. It is particularly important to improve salt tolerance in upland rice because of its growth environment. Upland rice landrace 17SM-19 with high salt tolerance was obtained from a previous study. In this study, an integrated analysis of transcriptome and metabolome was performed to determine the responses of the rice seedling to salt stress. When treated with 100 mm NaCl, the rice seedling growth was significantly inhibited at 5 d, with inhibition first observed in shoot dry weight (SDW). Changes in potassium (K+) content were associated with changes in SDW. In omics analyses, 1,900 differentially expressed genes (DEGs) and 659 differentially abundant metabolites (DAMs) were identified at 3 d after salt stress (DAS), and 1,738 DEGs and 657 DAMs were identified at 5 DAS. Correlation analyses between DEGs and DAMs were also conducted. The results collectively indicate that salt tolerance of upland rice landrace 17SM-19 seedlings involves many molecular mechanisms, such as those involved with osmotic regulation, ion balance, and scavenging of reactive oxygen species.
RESUMEN
Prolonged use of tight-fitting PPE, e.g., by COVID-19 healthcare workers leads to skin injuries. An important contributor is the shear exerted on the skin due to static friction at the skin-PPE interface. This study aims to develop an optimised wax-oil lubricant that reduces the friction, or shear, in the skin-PPE contact for up to four hours. Lubricants with different wax-oil combinations were prepared using beeswax, paraffin wax, olive oil, and mineral oil. In-vivo friction measurements involving seven participants were conducted by sliding a polydimethylsiloxane ball against the volar forearms to simulate the skin-PPE interface. The maximum static coefficient of friction was measured immediately and four hours after lubricant application. It was found that the coefficient of friction of wax-oil lubricants is mainly governed by the ratio of wax to oil and the thermal stability and morphology of the wax. To maintain long-term lubricity, it is crucial to consider the absorption of oil into the PPE material. The best performing lubricant is a mixture of 20 wt% beeswax, 40 wt% olive oil, and 40 wt% mineral oil, which compared to unlubricated skin, provides 87% (P = 0.0006) and 59% (P = 0.0015) reduction in instantaneous and 4-h coefficient of friction, respectively.
Asunto(s)
Lubricantes/química , Lubricantes/farmacología , Equipo de Protección Personal/efectos adversos , Piel/efectos de los fármacos , Ceras , Adulto , COVID-19 , Femenino , Antebrazo/fisiología , Humanos , Masculino , Aceite Mineral/química , Aceite de Oliva/química , Factores de Tiempo , Ceras/químicaRESUMEN
The bone quality has a significant effect on the insertion torque of dental implant. In most clinical studies, bone density is used as a gold standard in predicting insertion torque. By contrast, trabecular microstructure is ignored. In this study, a microstructure-based numerical computational method with high accuracy and efficiency for the insertion torque of dental implant was proposed by introducing two microscopic variables, namely, volume fraction and fabric tensor. First, two kinds of 3D microstructural solid models with same volume fraction and fabric tensor were established on the basis of the microstructural topology of six reference specimens. Second, a new numerical simulation method based on homogenous theory was used to explore the material models of these 3D microstructural solid models at the microscopic scale. Then, the anisotropic material models of specimens were developed on the basis of the mixture rule. Thereafter, a numerical simulation based on the anisotropic finite element (FE) model was carried out to acquire the insertion torque. To demonstrate the efficiency and accuracy of the simulation based on the anisotropic FE model, numerical simulations based on isotropic FE model and micro-computer tomography (micro-CT) FE models were also implemented as comparisons. Comparison of the simulated peak insertion torques of the anisotropic, isotropic, and micro-CT FE models with insertion experiments demonstrated the feasibility and potential of the proposed method. The anisotropic FE model reduced the time consumption by 91.85% and enhanced the accuracy by 11.82% compared with the micro-CT and isotropic FE models, respectively.
Asunto(s)
Simulación por Computador , Implantes Dentales , Torque , Anisotropía , Análisis de Elementos Finitos , Microtomografía por Rayos XRESUMEN
OBJECTIVE: To study the protection of puerarin on the cerebral injury in the rats with acute local ischemia. METHOD: Rat was evaluated model of acute local cerebral ischemia was made by ligating middle cerebral artery. The cerebral damage toxylin and eosin((HE). RESULT: The number of died neurons were significantly less in puerarin-treated rats than in the rats with cerebral ischemia (P < 0. 05). Similarly, the cerebral edema were significantly attenuated in the puerarin-treated rats as compared with cerebrally ischemic rats. CONCLUSION: Puerarin can prevent the neuron from damage induced by acute cerebral ischemia.
Asunto(s)
Isquemia Encefálica/patología , Corteza Cerebral/patología , Isoflavonas/farmacología , Fármacos Neuroprotectores/farmacología , Animales , Muerte Celular/efectos de los fármacos , Femenino , Infarto de la Arteria Cerebral Media/patología , Masculino , Neuronas/efectos de los fármacos , Ratas , Ratas Wistar , Vasodilatadores/farmacologíaRESUMEN
OBJECTIVE: To study the effect of puerarin on the expression of Hsp (heat shock protein) 70 in the rats with cerebral injury induced by acute local ischemia. METHOD: Rat model of acute local cerebral ischemia was made by ligating middle cerebral artery. The Hsp70 expression in brain tissue was detected by SP method of immunohistochemistry. RESULT: The expression of Hsp70 was significantly higher in puerarintreated rats than those in the rats with cerebral ischemia. CONCLUSION: Puerarin can enhance the level of Hsp70 expression in the rats with cerebral injury induced by acute local ischemia.
Asunto(s)
Isquemia Encefálica/metabolismo , Proteínas HSP70 de Choque Térmico/metabolismo , Isoflavonas/farmacología , Fármacos Neuroprotectores/farmacología , Pueraria , Animales , Isquemia Encefálica/etiología , Isquemia Encefálica/patología , Femenino , Infarto de la Arteria Cerebral Media/complicaciones , Isoflavonas/aislamiento & purificación , Masculino , Neuronas/metabolismo , Fármacos Neuroprotectores/aislamiento & purificación , Plantas Medicinales/química , Pueraria/química , Ratas , Ratas WistarRESUMEN
OBJECTIVE: To observe the effect of Puerarin Injection on the hemorheology in acute blood-stasis model rats. METHOD: The acute blood-stasis model rats were made by being soaked in ice water afer being injected adrenaline hydrochloride injection in a major dose. The changes of viscosity of whole blood and plasma, blood yield stress, erythrocyte aggregation and the maximum rate of platelet aggregation in the acute blood-stasis model rats were measured with Auto-Viscometer, and then the influence of Puerarin Injection on the hemorheology in the model rats was investigated. RESULT: The viscosity of whole blood and plasma, and blood yield stress in the acute blood-stasis model rats were significantly higher than those in the normal control group (P < 0.01, P < 0.05). Both the high dose and the low dose of Puerarin Injection could reduce the viscosity of whole blood and plasma, blood yield stress and the maximum rate of platelet aggregation in the acute blood-stasis model rats (P < 0.01, P < 0.05). The high dose could also reduce the erythrocyte aggregation and the deformed Index of red blood cell (P < 0.05). CONCLUSION: Puerarin Injection can ameliorate the hemorheology in acute blood-stasis model rats, and it has a dose-response relationship.