Analysis of Risk Factors for Severe Acute Pancreatitis in the Early Period (<24 h) After Admission.

BACKGROUND: Severe acute pancreatitis (SAP) has high mortality. Early identification of high-risk factors that may progress to SAP and active intervention measures may improve the prognosis of SAP patients. OBJECTIVE: Clinical data within 24 h after admission were retrospectively analyzed to provide an evidence for early screening of high-risk factors in patients with SAP. METHODS: A review of clinical data of acute pancreatitis patients from January 1, 2018, to December 31, 2022, was conducted. We compared the clinical data of SAP and non-SAP patients, and a multivariable logistic regression model was used to identify the independent predictors of SAP. The receiver operating characteristic (ROC) curve of SAP was drawn for continuous numerical variables to calculate the optimal clinical cutoff value of each variable, and the predictive value of each variable was compared by the area under the ROC curve. RESULTS: Based on the multivariate logistic regression analysis of Age (odds ratio (OR), 1.032;95% confident interval (CI),1.018-1.046, p < 0.001), body mass index (BMI) (OR, 1.181; 95% CI,1.083-1.288, p < 0.001), Non-HTGAP (nonhypertriglyceridemic acute pancreatitis) (OR, 2.098; 95% CI,1.276-3.45, p = 0.003), white blood cell count (WBC) (OR,1.072; 95% CI,1.034-1.111, p < 0.001), procalcitonin (PCT) (OR, 1.060; 95% CI, 1.027-1.095, p < 0.001), serum calcium (Ca) (OR,0.121; 95% CI, 0.050-0.292, p < 0.001), computed tomography severity index (CTSI) ≥4 (OR,12.942;95% CI,7.267-23.049, p < 0.001) were identified as independent risk factors for SAP. The area under the ROC curve (AUC) and optimal CUT-OFF values of continuous numerical variables for predicting SAP were Age (0.6079,51.5), BMI (0.6,23.25), WBC (0.6701,14.565), PCT (0.7086, 0.5175), Ca (0.7787,1.965), respectively. CONCLUSION: Age, BMI, non-HTGAP, WBC, PCT, serum Ca and CTSI≥4 have good predictive value for SAP.

Study on the Regeneration of Waste FCC Catalyst by Boron Modification.

Regeneration has been considered as an ideal way for the post-treatment of waste FCC catalyst (ECat). In this work, the degeneration mechanism of ECat was firstly researched and attributed to the increasing of strong acid sites accessibility of ECat in contrast with fresh FCC catalyst by adsorption FTIR. Based on the proposed degeneration mechanism, ECat was successfully regenerated through suitable weakening for strong acid sites by boron modification. Characterization and evaluation results suggested that, the strong acid sites of regenerated ECat (R-ECat) were apparently decreased by boron modification which had significantly improve the heavy oil catalytic cracking performance of R-ECat. Because of the excellent performance, R-ECat in this work could successfully substitute for partial fresh FCC catalyst in FCC unit, which would provide a practicable way for the reutilization of ECat.

Low threshold optical bistability based on topological edge state in photonic crystal heterostructure with Dirac semimetal.

The special band structure of three-dimensional Dirac semimetal (3D DSM) makes it show strong nonlinear optical characteristics in the terahertz region, which provides a new way to develop terahertz nonlinear devices with low threshold. In this paper, we theoretically study the optical bistability (OB) of transmitted light in a multilayer structure with 3D DSM embedded in two one-dimensional photonic crystals (1D PhC). The topological edge state (TES) excited by the 1D PhC heterostructure significantly enhances the local electric field near the nonlinear 3D DSM, which provides a positive condition for the realization of low threshold OB. Through parameter optimization, we obtain a threshold electric field with an incident electric field of 106 V/m levels. Furthermore, the influences of the Fermi energy and thickness of 3D DSM and the angle of the incident light on the hysteretic behavior as well as the threshold of OB are clarified. 3D DSM-based optical devices with intrinsic OB provide a building block for future integrated optical and all-optical networks.

Gauging the impacts of urbanization on CO2 emissions from the construction industry: Evidence from China.

The construction industry has aided rapid urbanization in China, significantly contributing to CO2 emissions. However, few studies have investigated the impacts of urbanization on CO2 emissions from the construction industry and the regional heterogeneity or considered the construction-related factors for urban construction scale to represent urbanization. To compensate for these limitations, this study aimed to explore the impacts of urbanization on CO2 emissions from the construction industry. Herein, the urban construction scale was used to represent urbanization, along with population size, economic growth, and technology level. An augmented Stochastic Impacts by Regression on Population, Affluence, and Technology model was used to estimate the cross-province panel data from three regions in China during 2008-2017. The heterogeneity due to regional differences in urbanization levels was addressed by classifying China into three regions- urbanized, urbanizing, and under-urbanized. The findings suggest that population size, economic growth, construction of residential buildings, and technology level were the primary factors impacting CO2 emissions, and the impact presented a declining trend from the urbanized to the urbanizing and under-urbanized regions. Specifically, an inverted U-shaped relationship existed between CO2 emissions and urban economic growth, and the urbanized region indicated a higher inflection point than other regions. The urbanization ratio was negatively correlated with CO2 emissions, while the energy intensity, per capita floor space of urban residential buildings, and per capita length of drainpipes were positively correlated with the CO2 emissions in all three regions. Further, the technology level was conducive to CO2 emissions reduction, however, it requires further improvement. The per capita area of paved roads exerted significantly negative effects in the urbanized region and insignificant in the urbanizing and under-urbanized regions. Overall, these results can help formulate policies to mitigate the construction industry's carbon emissions.

Platelet-derived growth factor-B signalling might promote epithelial-mesenchymal transition in gastric carcinoma cells through activation of the MAPK/ERK pathway.

INTRODUCTION: Epithelial-mesenchymal transition (EMT) is important in the metastasis of tumours and is triggered by several key growth factors, including platelet-derived growth factor-B (PDGF-B). But, whether PDGF-B signalling promotes EMT in gastric carcinoma cells is still unknown. MATERIAL AND METHODS: We established 2 gastric carcinoma cell lines (MKN28 and MKN45) to stably overexpress PDGF-B by lentiviral vectors, and expression of E-cadherin, N-cadherin, and ERK-1 were detected by western blot assay. Then, PDGF-B overexpression and normal MKN28 and MKN45 cells were cocultured with PDGFR-b positive fibroblast (hs738) and MAPK inhibitors were added; also, the expressions of ERK-1, E-cadherin, and N-cadherin were detected by western blot assay. RESULTS: After being cocultured with hs738 cells, expressions of ERK-1 and N-cadherin protein in PDGF-B overexpression MKN28 and MKN45 cells were much higher than normal MKN28 and MKN45 cells (p < 0.05), and those could be decreased by MAPK inhibitor. Also, expressions of E-cadherin protein in PDGF-B overexpression MKN28 and MKN45 cells were much lower than normal MKN28 and MKN45 cells (p < 0.05), and they could be increased by MAPK inhibitor. CONCLUSIONS: Our data indicate that PDGF-B signalling can induce EMT in gastric carcinoma cells. Thr tumour microenvironment is imperative in the process of PDGF-B signalling inducing EMT in gastric carcinoma cells. Also, activation of MAPK/ERK pathway, which is a downstream pathway of PDGF-B signalling, might participate in this process.

Unfolding interregional energy flow structure of China's construction sector based on province-level data.

The construction sector is a critical part in achieving energy conservation targets in China, as it accounts for approximately 30% of the annual national energy supply for building construction. Therefore, this study integrates multi-regional input-output analysis and ecological network analysis to track energy fluxes and pathways from the construction sector, aiming to facilitate the configuration of the energy-flow structure and improve understanding of the region's responsibilities. Results of a spatial distribution analysis show that the eastern area of China leads in fossil energy consumption (e.g., coal and crude oil), whereas western China is the largest consumer of natural gas. Spatial relationship analyss indicate that eastern areas are located at the top of the trophic structure, implying that these regions are prioritized in energy consumption over the surrounding regions. By contrast, most regions located in the northern parts of China are characterized by resource-abundant areas and are at the bottom of the trophic structure, thereby indicating their comparatively weak role in an exploitation relationship. An investigation of major metropolitan areas demonstrates that mandatory targets set by national instruments are stratified in accordance with their diverse role and status in energy consumption at the beginning of the 12th Five-Year Plan period. However, these targets remain insignificant in the context of the inner area.

Kerr Nonlinearity in germanium selenide nanoflakes measured by Z-scan and spatial self-phase modulation techniques and its applications in all-optical information conversion.

Germanium selenide (GeSe) has attracted considerable research interest due to its unique photoelectric characteristics: high abundance occurrence, low toxicity, high stability, and environmentally sustainable. To the best of our knowledge, less literature is available on the nonlinear optical (NLO) properties of GeSe and on its significance of the electronic structure. In this work, the GeSe nanoflake ethanol suspensions have been studied by using liquid phase exfoliation method and then characterized by Raman, transmission electron microscopy (TEM), transmittance and atomic force microscopy (AFM). The NLO properties of GeSe suspensions with different concentration are investigated by Z-scan and spatial self-phase modulation (SSPM) methods with continuous wave laser, which are coherent with the parameter nonlinear refractive index n2 and the third order nonlinear polarizabilities χ(3). The nonlinear refractive index n2 of GeSe dispersions basically occur in the order of 10-9 cm2/W for Z-scan methods and 10-6 cm2/W for SSPM technique, whereas the third-order nonlinear polarizabilities χ(3) total are within the range of 10-6 esu for SSPM method. On the basis of these substantial characteristics of the NLO response and high stability of the 2D GeSe, we have experimentally studied the applications of the GeSe suspensions on all-optical information conversion technique.

Sensitivity Enhancement of a Surface Plasmon Resonance Sensor with Platinum Diselenide.

The extraordinary optoelectronic properties of platinum diselenide (PtSe2), whose structure is similar to graphene and phosphorene, has attracted great attention in new rapidly developed two-dimensional (2D) materials beyond the other 2D material family members. We have investigated the surface plasmon resonance (SPR) sensors through PtSe2 with the transfer matrix method. The simulation results show that the anticipated PtSe2 biochemical sensors have the ability to detect analytic. It is evident that only the sensitivities of Ag or Au film biochemical sensors were observed at 118°/RIU (refractive index unit) and 130°/RIU, whereas the sensitivities of the PtSe2-based biochemical sensors reached as high as 162°/RIU (Ag film) and 165°/RIU (Au film). The diverse biosensor sensitivities with PtSe2 suggest that this kind of 2D material can adapt SPR sensor properties.

Downregulation of microRNA-193-3p inhibits tumor proliferation migration and chemoresistance in human gastric cancer by regulating PTEN gene.

In this study, we investigated the functional mechanisms of microRNA-193-3p (miR-193-3p) in human gastric cancer. Quantitative RT-PCR (qRT-PCR) was used to assess whether miR-193-3p was aberrantly expressed in gastric cancer cells and clinical samples from gastric cancer patients. Gastric cancer cell line AGS and MKN-45 cells were stably transduced with lentivirus to downregulate endogenous miR-193-3p. The modulation of miR-193-3p downregulation on gastric cancer proliferation, migration, chemo-drug responses, and tumor explant were assessed by MTT, wound-healing, 5-FU chemoresistance and in vivo tumorigenicity assays, respectively. Downstream target of miR-193-3p, phosphatase and tensin homolog (PTEN) in gastric cancer, was assessed by dual-luciferase reporter assay, qRT-PCR, and western blot. PTEN was knocked down by siRNA in AGS and MKN-45 cells to assess its direct impact on miR-193-3p modulation in gastric cancer. MiR-193-3p was aberrantly upregulated in both gastric cell lines and human gastric tumors. In AGS and MKN-45 cells, miR-193-3p downregulation reduced cancer proliferation, migration and 5-FU chemoresistance in vitro, and tumorigenicity in vivo. PTEN was confirmed to be targeted by miR-193-3p in gastric cancer. PTEN inhibition in AGS and MKN-45 cells directly reversed the anti-tumor modulations of miR-193-3p downregulation on gastric cancer proliferation, migration, and 5-FU chemoresistance. We presented clear evidence showing miR-193-3p played critical role in regulating human gastric cancer through direct targeting on PTEN gene.

Group technology empowering optimization of mixed-flow precast production in off-site construction.

Faced with immense pressure to reduce environmental impact, off-site construction (OSC) is considered a sustainable alternative to conventional practices. However, challenged by component diversity and a significant surge in demand, deficient or empirical-based scheduling management struggles to effectively harness the potential of mixed-flow precast production to improve efficiency, instead resulting in environmental impacts, and falling short of expected benefits in OSC projects. Therefore, this study addresses the conflict between efficiency and environmental impact arising from the application of mixed-flow precast production by integrating multi-objective optimization and group technology. A multi-objective optimization framework is proposed, incorporating grouping technology for mixed-flow precast production scheduling and aiming to minimize carbon emissions and reduce tardiness/earliness penalty. The non-dominated sorting genetic algorithm II (NSGA-II), adjusted by adaptive population initialization strategy and group technology, is introduced to solve this problem, striking a balance between sustainability and penalty costs. Through a real-case analysis, the proposed approach demonstrates an average reduction of 37.5% in carbon emissions compared to rule-based scheduling methods, a 30.1% reduction compared to previous research methods, along with over 10% reduction in tardiness/earliness penalty. This study enhances environmental benefits and efficiency from a production scheduling perspective and establishes an automated, practical method, fostering low-cost, high-efficiency green production for construction component enterprises, particularly for small and medium-sized enterprises, thereby promoting sustainable development in the construction industry.

Attachment of facile synthesized NaCo2O4 nanodots to SiO2 nanoflakes for sodium-rich boosted Pt-dominated ambient HCHO oxidation.

Surface alkali metal ions are typically utilized as available promoters for ambient HCHO oxidation. In this study, NaCo2O4 nanodots with two different preferential crystallographic orientations are synthesized by facile attachment to SiO2 nanoflakes with varying degrees of lattice defects. A unique Na-rich environment is established through interlayer Na+ diffusion based on the small size effect. The optimized catalyst Pt/HNaCo2O4/T2 can deal with HCHO below 5 ppm in the static measurement system with a sustained release background and produces approximately 40 ppm of CO2 in 2 h. Combining the experimental analyses with density functional theory (DFT) calculations, the possible catalytic enhancing mechanism is proposed from the support promotion perspective, and the positive synergistic effect of Na-rich, oxygen vacancies and optimized facets for Pt-dominant ambient HCHO oxidation via both kinetic and thermodynamic processes is confirmed.

A case report of a giant ileocecal cystic prolapse through the anus and literature review.

Intussusception refers to the invagination of a proximal loop of the bowel into an adjacent distal segment. This condition is rare in adults, especially when it involves a complete folding of the ileocecal area out of the body cavity. Meanwhile, enterogenous cysts are congenital malformations that are largely identified in childhood following symptoms of bowel obstruction. While surgical treatment is ultimately required for both diseases, deciding on the type of surgery and the right time to operate can be a challenge for clinicians. It is especially difficult to decide on treatment for an adult with the coincidental occurrence of both conditions and no definitive pathologic diagnosis prior to surgery. Here, we present the case study of a 19-year-old female patient who presented with a prolapsed anus due to intussusception caused by a large ileocecal mass. The patient was admitted to the emergency department with a "massive anal mass." She remained symptomatic after receiving conventional conservative treatment and had to undergo emergency surgery after developing an intestinal obstruction. While the patient's intraoperative condition also confirmed the preoperative CT findings, the situation became more complicated during surgery. The postoperative pathological report indicated the presence of an enterogenous cyst. After recovery from surgery, the patient was successfully discharged. Intussusception or intestinal obstruction caused by an intestinal mass is a surgical indication, and removal is the only way to cure the condition. This case study provides a helpful reference for general surgeons, especially anorectal surgeons, imaging physicians, and pathologists, and informs the diagnosis and treatment of this patient population.

Synthesis of Si-Modified Pseudo-Boehmite@kaolin Composite and Its Application as a Novel Matrix Material for FCC Catalyst.

Fluid catalytic cracking (FCC) has been the primary processing technology for heavy oil. Due to the inferior properties of heavy oil, an excellent performance is demanded of FCC catalysts. In this work, based on the acid extracting method, Si-modified pseudo-boehmite units (Si-PB) are constructed in situ and introduced into the structure of kaolin to synthesize a Si-PB@kaolin composite. The synthesized Si-PB@kaolin is further characterized and used as a matrix material for the FCC catalyst. The results indicate that, compared with a conventional kaolin matrix, a Si-PB@kaolin composite could significantly improve the heavy oil catalytic cracking performance of the prepared FCC catalyst because of its excellent properties, such as a larger surface area, a higher pore volume, and a good surface acidity. For the fresh FCC catalysts, compared with the FCC catalysts using conventional kaolin (Cat-1), the gasoline yield and total liquid yield of the catalyst containing Si-PB@kaolin (Cat-2) could obviously increase by 2.06% and 1.55%, respectively, with the bottom yield decreasing by 2.64%. After vanadium and nickel contamination, compared with Cat-1, the gasoline yield and total liquid yield of Cat-2 could increase by 1.97% and 1.24%, respectively, with the bottom yield decreasing by 1.80 percentage points.

The Research on Anti-Nickel Contamination Mechanism and Performance for Boron-Modified FCC Catalyst.

Fluid catalytic cracking (FCC) is still a key process in the modern refining area, in which nickel-contamination for an FCC catalyst could obviously increase the dry gas and coke yields and thus seriously affect the stability of the FCC unit. From the points of surface acidity modification and Ni-passivation, in this paper, a boron-modified FCC catalyst (BM-Cat) was prepared using the in situ addition method with B2O3 as a boron source and emphatically investigated its mechanism and performance of anti-nickel contamination. The mechanism research results suggested that, in calcination, boron could destroy the structure of the Y zeolite and thus decrease the total acid sites and strong acid sites of the Y zeolite from 291.5 and 44.6 µmol·g-1 to 244.2 and 32.1 µmol·g-1, respectively, which could obviously improve the dry gas and coke selectivity of the catalyst and thus enhance the nickel capacity for BM-Cat; on the other hand, under hydrothermal conditions, boron could react with NiO and form into NiB2O4, which could obviously raise the range of the reduction temperature for NiO from 350-600 °C to 650-800 °C and thus promote the nickel-passivation ability for BM-Cat. Therefore, evaluation results of heavy oil catalytic cracking indicated that, under the same nickel-contamination condition, in contrast to the compared catalyst (C-Cat), the dry gas yield, coke yield, and H2/CH4 of BM-Cat obviously decreased by 0.77 percentage points, 2.09 percentage points, and 13.53%, respectively, with light yield and total liquid yield increasing by 3.25 and 2.08 percentage points, respectively, which fully demonstrates the excellent anti-nickel contamination performance of BM-Cat.

Long Non-coding RNA FGD5-AS1 Regulates Cancer Cell Proliferation and Chemoresistance in Gastric Cancer Through miR-153-3p/CITED2 Axis.

BACKGROUND: In this study, we investigated the molecular mechanisms of human long non-coding RNA (lncRNA) FYVE RhoGEF And PH Domain Containing 5 Antisense RNA 1 (FGD5-AS1) and its downstream epigenetic axis, human microRNA-153-3p (hsa-miR-153-3p)/Cbp/P300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (CITED2) in human gastric cancer. METHODS: Gastric cancer cell lines and clinical tumor samples were used to assess FGD5-AS1 expression levels. Lentivirus containing FGD5-AS1 small interfering RNA (sh-FGD5AS1) was applied to knockdown FGD5-AS1 expression. Cancer cells in vitro and in vivo proliferation, and 5-FU chemoresistance were assessed, respectively. Expressions of hsa-miR-153-3p/CITED2 were also assessed in FGD5-AS1-downregulated gastric cancer cells. Hsa-miR-153-3p was knocked down and CITED2 was upregulated to assess their direct functional correlations with FGD5-AS1 in gastric cancer. RESULTS: Both gastric cancer cell lines and human tumor samples showed aberrant FGD5-AS1 upregulation. Lentiviral-induced FGD5-AS1 knockdown reduced cancer proliferation, 5-FU chemoresistance in vitro, and tumorigenicity in vivo. Hsa-miR-153-3p/CITED2 axis was confirmed to be downstream of FGD5-AS1 in gastric cancer. Hsa-miR-153-3p inhibition or CITED2 upregulation reversed the tumor-suppressing effects of FGD5-AS1 downregulation on gastric cancer proliferation and 5-FU chemoresistance. CONCLUSION: We demonstrated that FGD5-AS1 can regulate human gastric cancer cell functions, possibly through its downstream epigenetic axis of hsa-miR-153-3p/CITED2.

Vanadate-Rich BiOBr/Bi Nanosheets for Effective Adsorption and Visible-Light-Driven Photodegradation of Rhodamine B.

Two-dimensional (2D) BiOBr nanosheets (NSs) have attracted considerable interest as photocatalysts. The surface active sites of BiOBr NSs are crucial in determining the photocatalytic performance of these materials under visible light. The modification of the surface state of BiOBr NSs with multiple charged groups has been scarcely studied as a way to increase the number of surface active sites and the corresponding photocatalytic activity. Herein, vanadate-rich 2D BiOBr/Bi NSs were in-situ fabricated without adding strong reductants and subsequently used for visible-light-driven photocatalysis. Even under reductant-free condition, we were able to simultaneously deposit Bi0 and vanadate groups on the surface of pristine BiOBr NSs. The corresponding formation mechanism was also explored in a subsequent step. Compared to pristine BiOBr NSs and BiOBr/Bi NSs, the vanadate-rich BiOBr/Bi NSs prepared herein exhibited superior adsorption and enhanced photodegradation of Rhodamine B (RhB) under visible light illumination.

A Framework of Industrialized Building Assessment in China Based on the Structural Equation Model.

Compared with the conventional building, the industrialized building (IB) promotes the sustainable development of the construction industry, which will become a growth trend in the future. Nevertheless, the progress of industrialized building is intimately affected through the scientific evaluating mechanism, which still requires more research. Thus, this study establishes a conceptual framework of industrialized building assessment (IBA), which is validated through exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). The impact between efficiency and the other five dimensions are studied by the structural equations model (SEM). The findings indicated that the conceptual framework is valid, and the efficiency has a positive impact on economic factors, livability, safety, environmental factors, and social benefits. Consequently, the improvement of efficiency has turned out to be the primary issue for improving the growth of the industrialized building. This research explores the basic framework of industrialized building assessment and provides a basis to establish a comprehensive and precise industrial building evaluation mechanism in the near future.

Defect state of indium-doped bismuth molybdate nanosheets for enhanced photoreduction of chromium(vi) under visible light illumination.

The construction of defect states is an effective method for regulating the energy band structure of photocatalytic semiconductor materials. Establishing defect states effectively is a particularly significant strategy to improve the photoelectric conversion efficiency. Herein, we report a peculiar method to manufacture defect states in indium-doped Bi2MoO6 by changing the valence state of the doped indium element. By increasing the amount of doped indium, different doping forms and valence states are produced, which causes distortion of crystal structure, generation of oxygen vacancies and variation in the elemental valence state. Specifically, when Bi is substituted with In(3-x)+, the acceptor energy level becomes higher than the valence band due to the p-type-like doping, which widens the width of the valence band and drives the up-shift of the valence band edges, resulting in narrowed bandgap and improved photoresponse ability. In addition, the photoelectrochemical performance tests using the In-doped Bi2MoO6 show that low-valent indium doping effectively improves the carrier separation efficiency, reduces the transmission impedance, and greatly improves the photocatalytic reduction performance of Cr(vi). This study provides a new insight to the design of efficient photocatalysts for the green and sustainable treatment of Cr(vi) pollution.

B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma.

The development of biomarkers that accurately and reliably detect colorectal cancer is a promising approach for colorectal cancer screening. Therefore, the objective of the present study was to evaluate the protein expression of α-methylacyl-CoA racemase (P504S/AMACR), tumor protein p53 (p53), B-cell lymphoma 2 (Bcl-2) and Ki-67/mindbomb E3 ubiquitin protein ligase 1 (MIB-1) in a population of Chinese patients with colorectal carcinoma. Colorectal tumors with matched normal tissue margins were collected from 148 surgical patients, and the demographic and clinical characteristics were collected. Immunohistochemical staining and western blot analysis of P504S/AMACR, p53, Bcl-2 and Ki-67/MIB-1 were conducted. Statistical analyses were used to compare protein expression in the colorectal tumors and matched normal tissue margins and to identify any associations between them and various clinicopathological parameters. Survival analyses were performed using the Kaplan-Meier method. In the present study, immunohistochemistry and western blot analysis revealed significantly higher expression of all four proteins in colorectal tumors compared with matched normal tissue margins (P<0.001). Spearman's rank correlation analysis revealed that Bcl-2 expression was negatively correlated with pathological grade and Tumor-Node-Metastasis (TNM) stage (-0.827 and -0.388, respectively; P<0.05). Bcl-2 expression was revealed to be a significant prognostic indicator of colorectal carcinoma [relative risk (95% CI), 0.703 (0.552-0.895); P<0.05]. The log-rank test revealed a significant association between low Bcl-2 expression and reduced overall survival (P=0.039), as well as a significant association between older age (>55 years) and reduced overall survival (P<0.001) in Chinese patients with colorectal carcinoma. In conclusion, low expression of Bcl-2 is significantly correlated with advanced pathological grade and TNM stage and is a prognostic indicator of reduced overall survival in young Chinese patients with colorectal carcinoma.

Low-grade slightly elevated and polypoid colorectal adenomas display differential ß-catenin-TCF/LEF activity, c-Myc, and cyclin D1 expression.

AIM: To comparatively investigate the cellular and molecular characteristics of low-grade slightly elevated adenomas and polypoid adenomas. METHODS: Colorectal tumors were collected from 24 patients with slightly elevated adenomas and 23 patients with polypoid adenomas. Five commonly mutated genes (APC, BRAF, KRAS, NRAS, and PIK3CA) were selected for mutational analysis. Paraffin-embedded tumor sections were used to calculate the apoptotic index (AI) and Ki-67 labeling index (KLI). Two pure colorectal epithelial cell lines were created by pooling the slightly elevated and polypoid tumors. Western blots, luciferase assays for ß-catenin-T-cell factor protein/ß-catenin-lymphoid enhancer factor (ß-catenin-TCF/LEF)-driven transcriptional activity, and caspase activity assays were conducted on the two cell lines. RESULTS: Slightly elevated lesions showed a significantly lower APC mutational frequency and a significantly higher KRAS mutational frequency (both P < 0.05). Slightly elevated lesions showed a significantly lower AI (P < 0.05). ß-catenin and ß-catenin-TCF/LEF-driven transcriptional activity was significantly upregulated in slightly elevated lesions (both P < 0.05). In slightly elevated lesions, c-Myc was significantly downregulated, while cyclin D1 was significantly upregulated (both P < 0.05). ß-catenin-TCF/LEF-driven transcriptional activity was negatively correlated with c-Myc (ρ = -0.78). Slightly elevated lesions displayed significant Bcl-2 and Bcl-xL upregulation (both P < 0.05) along with significant decreases in caspase-9 and caspase-3 activity (both P < 0.05). c-Myc was negatively correlated with Bcl-2 and Bcl-xL (ρ = -0.74 and -0.78, respectively). CONCLUSION: The lower apoptotic activity of low-grade slightly elevated adenomas can be partly attributed to upregulated ß-catenin pathway activity and downregulated c-Myc expression.