KCNN1 promotes proliferation and metastasis of breast cancer via ERLIN2-mediated stabilization and K63-dependent ubiquitination of Cyclin B1.

Potassium Calcium-Activated Channel Subfamily N1 (KCNN1), an integral membrane protein, is thought to regulate neuronal excitability by contributing to the slow component of synaptic after hyperpolarization. However, the role of KCNN1 in tumorigenesis has been rarely reported, and the underlying molecular mechanism remains unclear. Here, we report that KCNN1 functions as an oncogene in promoting breast cancer cell proliferation and metastasis. KCNN1 was overexpressed in breast cancer tissues and cells. The pro-proliferative and pro-metastatic effects of KCNN1 were demonstrated by CCK8, clone formation, Edu assay, wound healing assay and transwell experiments. Transcriptomic analysis using KCNN1 overexpressing cells revealed that KCNN1 could regulate key signaling pathways affecting the survival of breast cancer cells. KCNN1 interacts with ERLIN2 and enhances the effect of ERLIN2 on Cyclin B1 stability. Overexpression of KCNN1 promoted the protein expression of Cyclin B1, enhanced its stability and promoted its K63 dependent ubiquitination, while knockdown of KCNN1 had the opposite effects on Cyclin B1. Knockdown (or overexpression) ERLNI2 partially restored Cyclin B1 stability and K63 dependent ubiquitination induced by overexpression (or knockdown) of KCNN1. Knockdown (or overexpression) ERLIN2 also partially neutralizes the effects of overexpression (or knockdown) KCNN1-induced breast cancer cell proliferation, migration and invasion. In paired breast cancer clinical samples, we found a positive expression correlations between KCNN1 and ERLIN2, KCNN1 and Cyclin B1, as well as ERLIN2 and Cyclin B1. In conclusion, this study reveals, for the first time, the role of KCNN1 in tumorigenesis and emphasizes the importance of KCNN1/ERLIN2/Cyclin B1 axis in the development and metastasis of breast cancer.

Coherence-OAM matrix properties of a twisted Gaussian Schell model beam.

Based on the cross-spectral density (CSD) function, the coherence-orbital angular momentum (COAM) matrix of twisted Gaussian Schell-model (TGSM) beam is proposed, and the COAM matrix is used to describe the correlation between OAM modes in TGSM optical field. The COAM matrix characteristics of TGSM beam are analyzed by numerical simulation. The results show that the COAM matrix characteristics of TGSM beam depend on the initial parameters of the beam. In addition, a method of generating TGSM beam by superposition of COAM matrix element modes is described, and the influence of different initial parameters on the superposition characteristics is studied. The results reveal the internal relationship between the coherent structure of the optical field, the twist phase and the OAM modes. Our work helps to explore new expressions of partially coherent beams and promote the practical application of optimizing partially coherent beams.

Effects of electron beam irradiation treatment on the structural and functional properties of okara insoluble dietary fiber.

BACKGROUND: Insoluble dietary fiber (IDF) has beneficial physiological effects, such as the promoting of intestinal peristalsis, the improving of intestinal flora, and the absorbing of some harmful substances. Okara, a byproduct of soybean processing, is a potential source of IDF. But the larger particle size and poor water solubility of okara IDF have adverse effects on sensory properties and functional characteristics. Therefore, we used an emerging type of physical method is electron beam irradiation (EBI) to modify okara, and investigated that the effects of EBI doses on the structure and functional properties of okara IDF. RESULTS: It was found that the electron beam treatment damaged the crystalline structure of IDF. Observation of the surface of EBI-treated IDF revealed a loose and porous morphology rather than the typical smooth structure. At a dose of 6 kGy, a smallest particle size and largest specific surface area of IDF was obtained, and these factors increased the apparent viscosity of an IDF dispersion. The water holding capacity, swelling capacity and the oil holding capacity upon irradiation at 6 kGy increased 74.13%, 84.76% and 41.62%, respectively. In addition, the capacity for adsorption of cholesterol, sodium cholate, glucose and nitrite ion were improved after electron beam treatment. CONCLUSION: The modified okara IDF showed improved particle sizes and hydration properties, and these changes correlated with an improvement to the rough taste of IDF and improvements to the texture and storage period upon supplementation into food. © 2022 Society of Chemical Industry.

A Disordered Rubik's Cube-Inspired Framework for Sodium-Ion Batteries with Ultralong Cycle Lifespan.

Sodium-ion batteries (SIBs) with fast-charge capability and long lifespan could be applied in various sustainable energy storage systems, from personal devices to grid storage. Inspired by the disordered Rubik's cube, here, we report that the high-entropy (HE) concept can lead to a very substantial improvement in the sodium storage properties of hexacyanoferrate (HCF). An example of HE-HCF has been synthesized as a proof of concept, which has achieved impressive cycling stability over 50 000 cycles and an outstanding fast-charging capability up to 75 C. Remarkable air stability and all-climate performance are observed. Its quasi-zero-strain reaction mechanism and high sodium diffusion coefficient have been measured and analyzed by multiple in situ techniques and density functional theory calculations. This strategy provides new insights into the development of advanced electrodes and provides the opportunity to tune electrochemical performance by tailoring the atomic composition.

Transcriptome analysis in patients with asthma after inhaled combination therapy with long-acting ß2-agonists and corticosteroids.

Introduction: Asthma is one of the major public health problems that imposes a great burden on societal, financial, and healthcare around the world. Asthma poorly affects the health-related quality of life and daily activities of patients. Treatment of asthma, including inhaled corticosteroids (ICS) and long-acting beta-agonists (LABAs), mainly aims to improve the lung function and reduce symptoms and exacerbations. Current treatment regimens are symptom-based strategies, and the status of airway inflammation after treatment is yet unknown. We conducted this study to understand the comprehensive inflammation or airway remodeling status of patients after ICS-LABA treatment through RNA transcriptome analysis. Materials and methods: Eight newly diagnosed asthmatic patients and two healthy subjects were recruited in this study. Asthmatic patients underwent blood tests, lung function test, and RNA transcriptome analysis before and after ICS-LABA treatment. Results: In comparison with healthy subjects, pretreatment asthmatic patients had higher expression of protein tyrosine kinase and related signaling pathways. After ICS-LABA treatment, the expression of nuclear receptor transcription coactivator, N-acetyltransferase, protein tyrosine kinase, nuclear receptor, and RNA polymerase II-activating transcription factor were downregulated. However, the post-treatment asthmatic patients still had higher expression of cysteine-type endopeptidase, endodeoxyribonuclease, apolipoprotein, and unfolded protein was still upregulated than healthy subjects. Conclusions: The combination of ICS/LABAs decreased airway inflammatory and remodeling pathways. However, allergen stimulation-related pathways were still upregulated in patients after ICS/LABA treatment. The combination of medication and allergen removal is a complete strategy for asthma.

Streamline Sulfur Redox Reactions to Achieve Efficient Room-Temperature Sodium-Sulfur Batteries.

It is vital to dynamically regulate S activity to achieve efficient and stable room-temperature sodium-sulfur (RT/Na-S) batteries. Herein, we report using cobalt sulfide as an electron reservoir to enhance the activity of sulfur cathodes, and simultaneously combining with cobalt single atoms as double-end binding sites for a stable S conversion process. The rationally constructed CoS2 electron reservoir enables the straight reduction of S to short-chain sodium polysulfides (Na2 S4 ) via a streamlined redox path through electron transfer. Meanwhile, cobalt single atoms synergistically work with the electron reservoir to reinforce the streamlined redox path, which immobilize in situ formed long-chain products and catalyze their conversion, thus realizing high S utilization and sustainable cycling stability. The as-developed sulfur cathodes exhibit a superior rate performance of 443 mAh g-1 at 5 A g-1 with a high cycling capacity retention of 80 % after 5000 cycles at 5 A g-1 .

Ultrathin 2D Mesoporous TiO2 /rGO Heterostructure for High-Performance Lithium Storage.

Lithium-ion batteries (LIBs) have been widely applied and studied as an effective energy supplement for a variety of electronic devices. Titanium dioxide (TiO2 ), with a high theoretical capacity (335 mAh g-1 ) and low volume expansion ratio upon lithiation, has been considered as one of the most promising anode materials for LIBs. However, the application of TiO2 is hindered by its low electrical conductivity and slow ionic diffusion rate. Herein, a 2D ultrathin mesoporous TiO2 /reduced graphene (rGO) heterostructure is fabricated via a layer-by-layer assembly process. The synergistic effect of ultrathin mesoporous TiO2 and the rGO nanosheets significantly enhances the ionic diffusion and electron conductivity of the composite. The introduced 2D mesoporous heterostructure delivers a significantly improved capacity of 350 mAh g-1 at a current density of 200 mA g-1 and excellent cycling stability, with a capacity of 245 mAh g-1 maintained over 1000 cycles at a high current density of 1 A g-1 . The in situ transmission electron microscopy analysis indicates that the volume of the as-prepared 2D heterostructures changes slightly upon the insertion and extraction of Li+ , thus contributing to the enhanced long-cycle performance.

Facile Synthesis of Hierarchical Hollow CoP@C Composites with Superior Performance for Sodium and Potassium Storage.

Hierarchical hollow CoP and carbon composites were obtained through a facile synthetic method, where carbonization and phosphorization of the precursor were completed within one single step. The composites are composed of hollow CoP@C spheres, which are further made up of CoP nanoparticles with a thin outer carbon layer. Electrochemical performances of the prepared CoP@C composites as anodes for sodium and potassium storage were evaluated and compared. In situ TEM, in situ synchrotron XRD, and DFT calculations were conducted to study the structural evolution and the interaction between Na/K and CoP during cycling processes. Benefiting from the synergistic effect of conductive carbon layer and hierarchical hollow structure, the as-prepared CoP@C composites demonstrate superior sodium and potassium storage capability as anode materials for rechargeable batteries.

A Direct Quantitative Analysis of Erythrocyte Intracellular Ionized Magnesium in Physiological and Pathological Conditions.

Magnesium (Mg2+) is an endogenous cation that is involved in many essential biological reactions. Abnormal Mg2+ metabolisms in the body affect important physiological and pathological processes. However, most endogenous Mg2+ markers fail to represent body Mg2+ status; they are disadvantageous in terms of representational capacity, applied range, operational convenience, etc. In this article, we evaluated some of the most popular Mg2+ marker candidates. A logical model of the blood Mg2+ compartments was established, which consisted of unstable Mg2+ pools, representative Mg2+ pools, and conserved Mg2+ pools. These pools were based on the metabolic efficiency of Mg2+ in an acute Mg2+ intake test. The results of this study showed that only the erythrocyte intracellular ionized Mg2+ (RBC [Mg2+]i), a representative Mg2+ pool, could effectively represent abnormal body Mg2+ metabolisms in various conditions, including dietary Mg2+ adjustments, aging and metabolic syndrome. These results suggest that RBC [Mg2+]i might be a widely applicable marker of body Mg2+ levels. On unified technology platform and evaluation system, this research compared the representative capacities of RBC [Mg2+]i, plasma Mg2+ concentration (plasma [Mg2+]), erythrocyte intracellular total Mg (RBC [Mg]total) and Mg retention in rats and mice under various Mg2+-metabolism-related physiological and pathological conditions. Our technique for the direct quantitative analysis of RBC [Mg2+]i may prove valuable for basic physiological research, dietary Mg2+ regulation, as well as clinical monitoring/intervention of Mg2+-metabolism-related pathology.

Nanocomposite Materials for the Sodium-Ion Battery: A Review.

Clean energy has become an important topic in recent decades because of the serious global issues related to the development of energy, such as environmental contamination, and the intermittence of the traditional energy sources. Creating new battery-related energy storage facilities is an urgent subject for human beings to address and for solutions for the future. Compared with lithium-based batteries, sodium-ion batteries have become the new focal point in the competition for clean energy solutions and have more potential for commercialization due to the huge natural abundance of sodium. Nevertheless, sodium-ion batteries still exhibit some challenges, like inferior electrochemical performance caused by the bigger ionic size of Na+ ions, the detrimental volume expansion, and the low conductivity of the active materials. To solve these issues, nanocomposites have recently been applied as a new class of electrodes to enhance the electrochemical performance in sodium batteries based on advantages that include the size effect, high stability, and excellent conductivity. In this Review, the recent development of nanocomposite materials applied in sodium-ion batteries is summarized, and the existing challenges and the potential solutions are presented.

Which is the Most Reasonable Anti-aging Strategy: Meta-analysis.

An organism's lifespan is inevitably accompanied by the aging process, which involves functional decline, a steady increase of a plethora of chronic diseases, and ultimately death. Thus, it has been an ongoing dream of mankind to improve health span and extend the lifespan. In the last century, there is a great increase in the search for eternal youth and an insatiable appetite for methods which could turn back the clock. Survival curves are key components of lifespan experiments. Many interventions have been reported to extend the lifespan, including the administration of pharmaceuticals, calorie restriction, and genetic alteration. However, few studies have attempted to provide a comprehensive analysis of the mechanism by which these various methods function to extend lifespan. We recently collected survival curves from published papers and recovered data by fitting models. The analysis results highlight the overall advantage of calorie restriction and its mimetics in aging and demonstrate that hypoglycemic agents and antioxidants have a superior effect on lifespan extension via a pattern of global integrity compared to other medications. This review provides a scientific foundation for the discovery of effective anti-aging agents and the formulation of scientific anti-aging strategies.

A novel exosome-based multifunctional nanocomposite platform driven by photothermal-controlled release system for repair of skin injury.

Currently, exosomes showed appropriate potential in the repair of skin injury. However, the functions of the exosomes could be compromised rapidly due to their short half-life and high clearance rate in vivo. In addition, the controlled release of effective concentrations of exosomes could increase the utilization efficiency of exosomes in wound healing. Accordingly, the design of an effective system for the controlled delivery of exosomes during the wound treatment period was necessary. In this contribution, we designed a novel exosome-based multifunctional nanocomposite platform with photothermal-controlled release performance for the repair of skin injury. Based on the agarose hydrogel, two-dimensional Ti3C2 (Ti3C2 MXene) and human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes, the as-prepared platform (i.e., hucMSC-derived exosome/Ti3C2 MXene hydrogel) was synthesized for the first time. Apart from possessing injectability, the hucMSC-derived exosome/Ti3C2 MXene hydrogel utilized the excellent photothermal effect of Ti3C2 MXene and proper phase transition performance of agarose hydrogel to provide a photothermal-controlled release system for the hucMSC-derived exosomes, which was beneficial for the personalized on-demand drug delivery. Importantly, the hucMSC-derived exosomes maintained their inherent structure and activity after being released from the Ti3C2 MXene hydrogel. Additionally, the as-prepared hydrogel with multifunctional performance also presented remarkable biocompatibility and photothermal-antibacterial property, and could efficiently accelerate wound healing by promoting cell proliferation, angiogenesis, collagen deposition, and reducing the level of inflammation at the wound site. The results suggested that the exosome-based multifunctional nanocomposite platform with great potential for wound healing would make significant advances in the revolution of traditional treatment methods in skin injury.

Structural Engineering of Prussian Blue Analogues Enabling All-Climate and Ultralong Cycling Sodium-Ion Batteries.

The development of cost-efficient, long-lifespan, and all-climate sodium-ion batteries is of great importance for advancing large-scale energy storage but is plagued by the lack of suitable cathode materials. Here, we report low-cost Na-rich Mn-based Prussian blue analogues with superior rate capability and ultralong cycling stability over 10,000 cycles via structural optimization with electrochemically inert Ni atoms. Their thermal stability, all-climate properties, and potential in full cells are investigated in detail. Multiple in situ characterizations reveal that the outstanding performances benefit from their highly reversible three-phase transformations and trimetal (Mn-Ni-Fe) synergistic effects. In addition, a high sodium diffusion coefficient and a low volume distortion of 2.3% are observed through in situ transmission electron microscopy and first-principles calculations. Our results provide insights into the structural engineering of Prussian blue analogues for advanced sodium-ion batteries in large-scale energy storage applications.

Lithium-Ion Charged Polymer Channels Flattening Lithium Metal Anode.

The concentration difference in the near-surface region of lithium metal is the main cause of lithium dendrite growth. Resolving this issue will be key to achieving high-performance lithium metal batteries (LMBs). Herein, we construct a lithium nitrate (LiNO3)-implanted electroactive ß phase polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) crystalline polymorph layer (PHL). The electronegatively charged polymer chains attain lithium ions on the surface to form lithium-ion charged channels. These channels act as reservoirs to sustainably release Li ions to recompense the ionic flux of electrolytes, decreasing the growth of lithium dendrites. The stretched molecular channels can also accelerate the transport of Li ions. The combined effects enable a high Coulombic efficiency of 97.0% for 250 cycles in lithium (Li)||copper (Cu) cell and a stable symmetric plating/stripping behavior over 2000 h at 3 mA cm-2 with ultrahigh Li utilization of 50%. Furthermore, the full cell coupled with PHL-Cu@Li anode and LiFePO4 cathode exhibits long-term cycle stability with high-capacity retention of 95.9% after 900 cycles. Impressively, the full cell paired with LiNi0.87Co0.1Mn0.03O2 maintains a discharge capacity of 170.0 mAh g-1 with a capacity retention of 84.3% after 100 cycles even under harsh condition of ultralow N/P ratio of 0.83. This facile strategy will widen the potential application of LiNO3 in ester-based electrolyte for practical high-voltage LMBs.

Analysis of Vibration-Damping Characteristics and Parameter Optimization of Cylindrical Cavity Double-Plate Phononic Crystal.

For the application of low-frequency vibration damping in industry, a cylindrical cavity double-layer plate-type local resonance phononic crystal structure is proposed to solve low-frequency vibration in mechanical equipment. Initially, using COMSOL 5.4 software, the bending wave band gap is calculated in conjunction with elastic dynamics theory and the BOLOCH theorem to be 127-384 Hz. Then the mechanism of bending wave gap is analyzed by combining element mode shape and an equivalent model. Subsequently, the bending vibration transmission characteristics of the crystal plate are explained, and the vibration-damping characteristics are illustrated in combination with the time-frequency domain. An experimental system is constructed to verify the vibration-damping properties of crystal plates; the experimental results and simulation results are verified with each other. Finally, the element structural parameters are optimized using the RSM. Fifty-four sets of experiments are designed based on six structural factors and three levels, and the expressions between the bending wave band gap and six structural factors are obtained. Combining the particle swarm algorithm, the optimization is performed with the band gap width as the target. This method is shown to be more accurate than the commonly used interior point method. The structure of cylindrical-cavity-type phononic crystal and the parameter optimization method proposed in this paper provide a certain reference for the design of local-resonance-type phononic crystal.

Achieving All-Plateau and High-Capacity Sodium Insertion in Topological Graphitized Carbon.

Hard carbon anodes with all-plateau capacities below 0.1 V are prerequisites to achieve high-energy-density sodium-ion storage, which holds promise for future sustainable energy technologies. However, challenges in removing defects and improving the insertion of sodium ions head off the development of hard carbon to achieve this goal. Herein, a highly cross-linked topological graphitized carbon using biomass corn cobs through a two-step rapid thermal-annealing strategy is reported. The topological graphitized carbon constructed with long-range graphene nanoribbons and cavities/tunnels provides a multidirectional insertion of sodium ions whilst eliminating defects to absorb sodium ions at the high voltage region. Evidence from advanced techniques including in situ XRD, in situ Raman, and in situ/ex situ transmission electron microscopy (TEM) indicates that the sodium ions' insertion and Na cluster formation occurred between curved topological graphite layers and in the topological cavity of adjacent graphite band entanglements. The reported topological insertion mechanism enables outstanding battery performance with a single full low-voltage plateau capacity of 290 mAh g-1 , which is almost 97% of the total capacity.

Selective Laser Melted Magnesium Alloys: Fabrication, Microstructure and Property.

As the lightest metal structural material, magnesium and its alloys have the characteristics of low density, high specific strength and good biocompatibility, which gives magnesium alloys broad application prospects in fields of biomedicine, transportation, and aerospace. Laser selective melting technology has the advantages of manufacturing complex structural parts, high precision and high degree of freedom. However, due to some disadvantages of magnesium alloy, such as low boiling point and high vapor pressure, the application of it in laser selective melting was relatively undeveloped compared with other alloys. In this paper, the fabrication, microstructure, mechanical performance and corrosion resistance property of magnesium alloys were summarized, and the potential applications and the development direction of selective laser melting magnesium alloys in the future are prospected.

Effects of high-pressure homogenization on physicochemical and functional properties of enzymatic hydrolyzed soybean protein concentrate.

This paper investigates the effect on the physicochemical and functional properties of soybean protein concentrate (SPC) by using Alcalase protease and high-pressure homogenization (HPH) (0, 20, 40, 60, 80, and 100 MPa) for the combined modification. The results showed that the degree of hydrolysis of SPC was 4.1% and the antigen protein was degraded after Alcalase hydrolysis, when the homogenization pressure (HP) was 6 0Mpa, the particle size of the SPC was the smallest, the zate potential absolute value up to 33.45 mV, the secondary structure has the lowest ß-sheet content, the highest random coil content, and the highest surface hydrophobicity (H0), the size of protein fragments on the microstructure surface is the smallest, the lowest denaturation temperature (T d ) and enthalpy (△H) are 72.59°C and 1.35 J/g, the highest solubility is 80.54%, and the highest water and oil holding capacities are 7.73 g/g and 6.51 g/g, respectively. The best emulsifying activity and emulsifying stability were 43.46 m2/g and 190.35 min, the most even distribution of emulsion droplets. This indicates that the HPH treatment destroys the structure of enzymatic hydrolyzed SPC, changes its physicochemical properties, and improves its functional properties. In this study, SPC was modified by HPH and enzyme combined treatment, in order to improve the functionality and application range of SPC, and provide a theoretical basis for its high-value utilization in the food field.

Factors and Outcomes Associated with Failed Noninvasive Positive Pressure Ventilation in Patients with Acute Respiratory Failure.

Background: The decision guild for non-invasive positive pressure ventilation (NPPV) application in acute respiratory failure (ARF) patients still needs to work out. Methods: Adult patients with acute hypoxemic or hypercapnic respiratory failure were recruited and treated with NPPV or primary invasive mechanical ventilation (IMV). Patients' characteristic and clinical outcomes were recorded. Logistic regression models were used to estimate the adjusted odds ratio (aOR) and 95% confidence intervals for baseline characteristics and clinical outcomes. Subgroup analyses by reason behind successful NPPV were conducted to ascertain if any difference could influence the outcome. Results: A total of 4525 ARF patients were recruited in our facility between year 2015 and 2017. After exclusion, 844 IMV patients, 66 patients with failed NPPV, and 74 patients with successful NPPV were enrolled. Statistical analysis showed APACHE II score (aOR = 0.93), time between admission and start NPPV (aOR = 0.92), and P/F ratio (aOR = 1.04) were associated with successful NPPV. When comparing with IMV patients, failed NPPV patients displayed a significantly lower APACHE II score, higher Glasgow Coma Scale, longer length of stay in hospital, longer duration of invasive ventilation, RCW/Home ventilator, and some comorbidities. Conclusion: APACHE II score, time between admission and start NPPV, and PaO2 can be predictors for successful NPPV. The decision of NPPV application is critical as ARF patients with failed NPPV have various worse outcomes than patients receiving primary IMV.

Ample dietary fat reduced the risk of primary vesical calculi by inducing macrophages to engulf budding crystals in mice.

Although primary vesical calculi is an ancient disease, the mechanism of calculi formation remains unclear. In this study, we established a novel primary vesical calculi model with d,l-choline tartrate in mice. Compared with commonly used melamine and ethylene glycol models, our model was the only approach that induced vesical calculi without causing kidney injury. Previous studies suggest that proteins in the daily diet are the main contributors to the prevention of vesical calculi, yet the effect of fat is overlooked. To assay the relationship of dietary fat with the formation of primary vesical calculi, d,l-choline tartrate-treated mice were fed a high-fat, low-fat, or normal-fat diet. Genetic changes in the mouse bladder were detected with transcriptome analysis. A high-fat diet remarkably reduced the morbidity of primary vesical calculi. Higher fatty acid levels in serum and urine were observed in the high-fat diet group, and more intact epithelia in bladder were observed in the same group compared with the normal- and low-fat diet groups, suggesting the protective effect of fatty acids on bladder epithelia to maintain its normal histological structure. Transcriptome analysis revealed that the macrophage differentiation-related gene C-X-C motif chemokine ligand 14 (Cxcl14) was upregulated in the bladders of high-fat diet-fed mice compared with those of normal- or low-fat diet-fed mice, which was consistent with histological observations. The expression of CXCL14 significantly increased in the bladder in the high-fat diet group. CXCL14 enhanced the recruitment of macrophages to the crystal nucleus and induced the transformation of M2 macrophages, which led to phagocytosis of budding crystals and prevented accumulation of calculi. In human bladder epithelia (HCV-29) cells, high fatty acid supplementation significantly increased the expression of CXCL14. Dietary fat is essential for the maintenance of physiological functions of the bladder and for the prevention of primary vesical calculi, which provides new ideas for the reduction of morbidity of primary vesical calculi.